A TGF-? mediated regulatory mechanism modulates the T cell immune response to rotavirus in adults but not in children

Children with acute RV-gastroenteritis (GE) had low or undetectable levels of circulating IFN-?+, IL-13+, IL-2+, IL-10+ or IL-17+ RV-T cells. IFN-?+ T cells and low frequencies of IL-10+ and IL-2+ CD4+ T cells were found in adults with RV-GE during acute and convalescence phases, respectively. Circulating single IFN-?+ > double IFN-?+/IL-2+ > single IL-2+RV-CD4+T cells were observed in healthy adults. In this group, frequencies of IFN-?+ RV-T cells increased after removing CD25+cells, blocking TGF-? with its natural inhibitor, LAP, or inhibiting TGF-?RI signalling pathway with ALK5i. The frequencies of IFN-?+ RV-T cells were also incremented in PBMC depleted of CD25+cells and treated with ALK5i, suggesting that TGF? inhibition may be independent of Treg cells. The ALK5i effect was observed in adults but not in children with RV-GE, who had normal numbers of TGF-?+ Treg cells. Thus, a TGF-?-mediated regulatory mechanism that modulates RV-T cells in adults is not evident in children.

• Fecha de publicación: 2010
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/27853
• Acceso en línea: https://doi.org/10.1016/j.virol.2009.12.016; https://repository.urosario.edu.co/handle/10336/27853
• Palabra clave: Rotavirus; Humans; Interferon-gamma; Interleukin 13; Interleukin-2; Interleukin-10; Interleukin-17; Transforming growth factor betaT-lymphocytes; T-lymphocytes; regulatory
• License: Abierto (Texto Completo)