Anti-group A streptococcal vaccine epitope : Structure, stability, and its ability to interact with HLA class II molecules

Streptococcus pyogenes infections remain a health problem in several countries due to poststreptococcal sequelae. We developed a vaccine epitope (StreptInCor) composed of 55 amino acids residues of the C-terminal portion of the M protein that encompasses both T and B cell protective epitopes. The nuclear magnetic resonance (NMR) structure of the StreptInCor peptide showed that the structure was composed of two microdomains linked by an 18-residue α-helix. A chemical stability study of the StreptInCor folding/unfolding process using far-UV circular dichroism showed that the structure was chemically stable with respect to pH and the concentration of urea. The T cell epitope is located in the first microdomain and encompasses 11 out of the 18 α-helix residues, whereas the B cell epitope is in the second microdomain and showed no α-helical structure. The prediction of StreptInCor epitope binding to different HLA class II molecules was evaluated based on an analysis of the 55 residues and the theoretical possibilities for the processed peptides to fit into the P1, P4, P6, and P9 pockets in the groove of several HLA class II molecules. We observed 7 potential sites along the amino acid sequence of StreptInCor that were capable of recognizing HLA class II molecules (DRB1*, DRB3*, DRB4*, and DRB5*). StreptInCor- overlapping peptides induced cellular and humoral immune responses of individuals bearing different HLA class II molecules and could be considered as a universal vaccine epitope. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

• Fecha de publicación: 2011
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/21890
• Acceso en línea: https://doi.org/10.1074/jbc.M110.132118; https://repository.urosario.edu.co/handle/10336/21890
• Palabra clave: Ciencias médicas, Medicina; Química & ciencias afines; controlled study; human; Biomolecular; B-Lymphocyte; Secondary; Tertiary; T-Lymphocyte; Humans; Amino acid sequence; B cells; Class II; Helical structures; Helix residues; Humoral immune response; Micro-domains; Microdomain; Potential sites; Streptococcus pyogenes; T-cell epitopes; Terminal portions; UV circular dichroism; Amino acids; Chemical stability; Dichroism; Molecules; Nuclear magnetic resonance; Peptides; Resonance; Urea; Vaccines; Antigens; Amino acid; Epitope; HLA antigen class 2; HLA DR antigen; M protein; Peptide; Streptincor; Streptococcus vaccine; Unclassified drug; Urea; Alpha helix; Alpha helix; Amino acid sequence; Antigen binding; Antigen recognition; Article; Carboxy terminal sequence; Cellular immunity; Circular dichroism; Human tissue; Humoral immunity; Molecular interaction; Molecular stability; Nuclear magnetic resonance; pH; Prediction; Priority journal; Protein domain; Protein folding; Protein structure; Streptococcus pyogenes; Antigen Presentation; Epitopes; Epitopes; Histocompatibility Antigens Class II; Nuclear Magnetic Resonance; Protein Stability; Protein Structure; Protein Structure; Streptococcal Infections; Streptococcal Vaccines; Streptococcus pyogenes
• License: Abierto (Texto Completo)