Temporal gene expression in the hippocampus and peripheral organs to endotoxin-induced systemic inflammatory response in caspase-1-deficient mice

Objectives: Caspase-1 (casp1), a key protease involved in the systemic inflammatory response syndrome (SIRS), controls the brain expression of a set of eight genes: Nos2 and Ptgs2 (nitric oxide synthase 2 and prostaglandin-endoperoxide synthase 2, two inducible enzymes), Cxcl1 and Cxcl10 (C-X-C motif chemokine ligand 1 and ligand 10), Tgtp and Gbp2 (T cell-specific GTPase 1 and guanylate-binding protein 2, two GTPases), Adamts1 (a disintegrin-like and metallopeptidase with thrombospondin type 1 motif, 1, a metalloprotease) and Il1rn (interleukin-1 receptor antagonist). Our objective was to ascertain whether casp1 also controlled the peripheral expression of these genes and, if so, to compare their central versus peripheral patterns of gene expression in immune and endocrine tissues during SIRS. Methods: Wild-type (wt) and casp1 knockout (casp1-/-) mice were injected with either saline or a high dose of endotoxin/lipopolysaccharide (LPS; 800 ?g/mice i.p.). Saline-injected mice were immediately euthanized after injection, whereas LPS-injected mice were sacrificed 6 and 12 h after LPS administration. Hippocampal, splenic and adrenal gene expressions were determined by real-time PCR. Results: Overall, casp1-/- mice showed a lower inflammatory response than wt mice. The expression levels of powerful proinflammatory factors such as Nos2 and Ptgs2 was reduced in casp1-/- mice. Moreover, a hierarchical clustering analysis aimed at studying patterns of gene coexpression revealed large alterations in the hippocampal pattern of casp1-/- mice. Surprisingly, the expression of Adamts1 was increased in the hippocampus and adrenals of casp1-/- mice. Conclusions: The resilience of casp1-/- mice to SIRS lethality is associated with a lower inflammatory response, loss of hippocampal gene coexpression patterns, and increased hippocampal Adamts1 gene expression. The latter might be beneficial for casp1-/- mice, since ADAMTS1 is likely to play a role in neuronal plasticity. The mechanisms described here may help the development of either novel biomarkers or therapeutic targets against SIRS/sepsis. © 2015 S. Karger AG, Basel.

• Fecha de publicación: 2015
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/22651
• Acceso en línea: https://doi.org/10.1159/000368310; https://repository.urosario.edu.co/handle/10336/22651
• Palabra clave: Endotoxin; Escherichia coli lipopolysaccharide; Sodium chloride; Interleukin 1beta converting enzyme; Lipopolysaccharide; Adamts1 gene; Adrenal gland; Animal experiment; Animal model; Animal tissue; Article; Caspase 1 gene; Controlled study; Correlation coefficient; Cxc motif ligand 1 gene; Cxc motif ligand 10 gene; Disintegrin like and metallopeptidase with thrombospondin type 1 motif 1 gene; Gene expression profiling; Gene inactivation; Gene interaction; Genetic association; Guanylate binding protein 2 gene; Hippocampus; Immune response gene; Immunogenetics; Inducible nitric oxide synthase gene; Inflammation; Injection; Interleukin 1 receptor antagonist gene; Lethality; Male; Mouse; Nerve cell plasticity; Nonhuman; Priority journal; Prostaglandin endoperoxide synthase 2 gene; Real time polymerase chain reaction; Spleen; Systemic inflammatory response syndrome; T cell specific gtpase 1 gene; Wild type; Adrenal gland; Animal; C57bl mouse; Chemically induced; Deficiency; Disease model; Gene expression; Genetics; Hippocampus; Knockout mouse; Metabolism; Spleen; Systemic inflammatory response syndrome; Adrenal glands; Animals; Caspase 1; Gene expression; Hippocampus; Lipopolysaccharides; Male; Mice; Spleen; Systemic inflammatory response syndrome; Adamts1; Chemokines; Coexpression; Cox2; Cxcl1; Cxcl10; Inflammation; Lipopolysaccharide; Nos2; Transcription; animal; knockout; inbred c57bl; Disease models; Mice; Mice
• License: Abierto (Texto Completo)