Evaluation of the analytical and diagnostic performance of a digital droplet polymerase chain reaction (ddPCR) assay to detect Trypanosoma cruzi DNA in blood samples

Background: The recent development of novel Polymerase Chain Reaction (PCR) technologies that confer theoretical advantages over quantitative PCR has considerable potential in the diagnosis of low load infections, such as Trypanosoma cruzi in the chronic phase of Chagas disease. We evaluated the uti...

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Autores:

Tipo de recurso:

Fecha de publicación:

2018

Institución:

Universidad del Rosario

Repositorio:

Repositorio EdocUR - U. Rosario

Idioma:

eng

OAI Identifier:

oai:repository.urosario.edu.co:10336/22821

Acceso en línea:

https://doi.org/10.1371/journal.pntd.0007063
https://repository.urosario.edu.co/handle/10336/22821

Palabra clave:

Adult
Blood sampling
Chagas disease
Child
Controlled study
Diagnostic test accuracy study
Dna extraction
Droplet digital polymerase chain reaction
Enzyme linked immunosorbent assay
Epimastigote
Fever
Gene dosage
Hemagglutination inhibition test
Hemagglutination test
Hepatomegaly
Human
Immunofluorescence test
Life cycle stage
Limit of detection
Nonhuman
Parasite load
Parasitological parameters
Predictive value
Real time polymerase chain reaction
Reproducibility
Sensitivity and specificity
Splenomegaly
Tandem repeat
Trypanosoma cruzi
Trypomastigote
Blood
Chagas disease
Classification
Diagnostic test
Evaluation study
Genetics
Isolation and purification
Parasitology
Procedures
Trypanosoma cruzi
Protozoal dna
Chagas disease
Humans
Real-time polymerase chain reaction
Sensitivity and specificity
Trypanosoma cruzi
Routine
Protozoan
Diagnostic tests
Dna

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