A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies

Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestrati...

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Autores:
Tipo de recurso:
Fecha de publicación:
2013
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/8765
Acceso en línea:
https://doi.org/10.1371/journal.pone.0052679
http://repository.urosario.edu.co/handle/10336/8765
Palabra clave:
Malaria
Inmunología
PfEMP1
Plasmodium
Erythrocyte membrane protein 1
protozoan
Protein antibody
Molecular
Preschool
Strain transcending antibody
Unclassified drug
Amino acid sequence
Antigen antibody reaction
Antigen binding
Child
Disease severity
Erythrocyte
Flow cytometry
Immunofluorescence test
In vitro study
Plasmodium falciparum
Preschool child
Protein binding
Protein domain
Protein motif
Amino acid motifs
Erythrocytes
Immunoglobulin g
Molecular sequence data
Plasmodium falciparum
Plasmodium falciparum
Rights
License
Abierto (Texto completo)
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dc.title.spa.fl_str_mv A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
spellingShingle A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
Malaria
Inmunología
PfEMP1
Plasmodium
Erythrocyte membrane protein 1
protozoan
Protein antibody
Molecular
Preschool
Strain transcending antibody
Unclassified drug
Amino acid sequence
Antigen antibody reaction
Antigen binding
Child
Disease severity
Erythrocyte
Flow cytometry
Immunofluorescence test
In vitro study
Plasmodium falciparum
Preschool child
Protein binding
Protein domain
Protein motif
Amino acid motifs
Erythrocytes
Immunoglobulin g
Molecular sequence data
Plasmodium falciparum
Plasmodium falciparum
title_short A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title_full A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title_fullStr A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title_full_unstemmed A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
title_sort A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
dc.subject.decs.spa.fl_str_mv Malaria
Inmunología
PfEMP1
Plasmodium
topic Malaria
Inmunología
PfEMP1
Plasmodium
Erythrocyte membrane protein 1
protozoan
Protein antibody
Molecular
Preschool
Strain transcending antibody
Unclassified drug
Amino acid sequence
Antigen antibody reaction
Antigen binding
Child
Disease severity
Erythrocyte
Flow cytometry
Immunofluorescence test
In vitro study
Plasmodium falciparum
Preschool child
Protein binding
Protein domain
Protein motif
Amino acid motifs
Erythrocytes
Immunoglobulin g
Molecular sequence data
Plasmodium falciparum
Plasmodium falciparum
dc.subject.keyword.eng.fl_str_mv Erythrocyte membrane protein 1
protozoan
Protein antibody
Molecular
Preschool
Strain transcending antibody
Unclassified drug
Amino acid sequence
Antigen antibody reaction
Antigen binding
Child
Disease severity
Erythrocyte
Flow cytometry
Immunofluorescence test
In vitro study
Plasmodium falciparum
Preschool child
Protein binding
Protein domain
Protein motif
Amino acid motifs
Erythrocytes
Immunoglobulin g
Molecular sequence data
Plasmodium falciparum
Plasmodium falciparum
description Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface
publishDate 2013
dc.date.created.none.fl_str_mv 2013-01-15
dc.date.issued.none.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2014-08-11T14:48:06Z
dc.date.available.none.fl_str_mv 2014-08-11T14:48:06Z
dc.type.eng.fl_str_mv article
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1371/journal.pone.0052679
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.uri.none.fl_str_mv http://repository.urosario.edu.co/handle/10336/8765
url https://doi.org/10.1371/journal.pone.0052679
http://repository.urosario.edu.co/handle/10336/8765
identifier_str_mv 1932-6203
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationTitle.none.fl_str_mv PLOS ONE
dc.relation.citationVolume.none.fl_str_mv Vol. 8
dc.relation.ispartof.spa.fl_str_mv PLOS ONE, ISSN 1932-6203, V. 8 N. 1 Jan, 2013
dc.relation.uri.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052679
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto completo)
rights_invalid_str_mv Abierto (Texto completo)
http://purl.org/coar/access_right/c_abf2
dc.format.medium.spa.fl_str_mv Recurso electrónico
dc.format.mimetype.none.fl_str_mv application/pdf
dc.format.tipo.spa.fl_str_mv Documento
dc.publisher.spa.fl_str_mv Universidad del Rosario
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
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spelling Comunidad Rosarista4337b0a3-0238-4c14-bf8b-8b98d2e9908b6000f56ff8d-4ec1-4d07-a27e-c0e6e805eee660078b2f455-da24-4179-9e9b-cf47f469384a6007333b7bb-1950-4186-9858-b8b1a9896af26004d5e3094-2e0c-422e-a290-a7c176a03875600ab65f82c-df13-44e4-9b71-f3d272750d78600c80d29d7-0466-42aa-b3ea-248382006932600f007bd51-2df9-4895-ba8e-2fb0080c5a4e6002014-08-11T14:48:06Z2014-08-11T14:48:06Z2013-01-152013Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surfaceRecurso electrónicoapplication/pdfDocumentohttps://doi.org/10.1371/journal.pone.00526791932-6203http://repository.urosario.edu.co/handle/10336/8765engUniversidad del RosarioNo. 1PLOS ONEVol. 8PLOS ONE, ISSN 1932-6203, V. 8 N. 1 Jan, 2013https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052679Abierto (Texto completo)EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMalariaInmunologíaPfEMP1PlasmodiumErythrocyte membrane protein 1protozoanProtein antibodyMolecularPreschoolStrain transcending antibodyUnclassified drugAmino acid sequenceAntigen antibody reactionAntigen bindingChildDisease severityErythrocyteFlow cytometryImmunofluorescence testIn vitro studyPlasmodium falciparumPreschool childProtein bindingProtein domainProtein motifAmino acid motifsErythrocytesImmunoglobulin gMolecular sequence dataPlasmodium falciparumPlasmodium falciparumA Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending AntibodiesarticleArtículohttp://purl.org/coar/resource_type/c_6501Blomqvist, KarinAlbrecht, LetusaQuintana, Maria del PilarAngeletti, DavideJoannin, NicolasChêne, ArnaudMoll, KirstenWahlgren, MatsBlomqvist, KarinAlbrecht, LetusaQuintana, Maria del PilarAngeletti, DavideJoannin, NicolasChêne, ArnaudMoll, KirstenWahlgren, MatsORIGINALA Sequence in Subdomain 2 of DBL1a.pdfA Sequence in Subdomain 2 of DBL1a.pdfapplication/pdf1134717https://repository.urosario.edu.co/bitstreams/ee94c6bb-ee01-4074-a7ea-223adc102bd8/download4edbfa625c0a482702b50a09187490daMD51LICENSElicense.txtlicense.txttext/plain2156https://repository.urosario.edu.co/bitstreams/99936216-b115-4402-977d-679cb81959a4/downloadb4f8fe66e94b897ab4c355bac005ad16MD52TEXTA Sequence in Subdomain 2 of DBL1a.pdf.txtA Sequence in Subdomain 2 of DBL1a.pdf.txtExtracted texttext/plain61756https://repository.urosario.edu.co/bitstreams/dbb995d1-a0f4-4b37-9790-b1763cdfee27/downloadb62ae89570acac99c1297417715308b1MD59THUMBNAILA Sequence in Subdomain 2 of DBL1a.pdf.jpgA Sequence in Subdomain 2 of DBL1a.pdf.jpgGenerated Thumbnailimage/jpeg4909https://repository.urosario.edu.co/bitstreams/0d2e4d27-4c0e-41a0-bd4b-621c222e4e34/downloadb9ea5aa55f7d8910139cd04ed01512f5MD51010336/8765oai:repository.urosario.edu.co:10336/87652022-08-31 12:44:56.677https://repository.urosario.edu.coRepositorio institucional 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