A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestrati...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2013
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/8765
- Acceso en línea:
- https://doi.org/10.1371/journal.pone.0052679
http://repository.urosario.edu.co/handle/10336/8765
- Palabra clave:
- Malaria
Inmunología
PfEMP1
Plasmodium
Erythrocyte membrane protein 1
protozoan
Protein antibody
Molecular
Preschool
Strain transcending antibody
Unclassified drug
Amino acid sequence
Antigen antibody reaction
Antigen binding
Child
Disease severity
Erythrocyte
Flow cytometry
Immunofluorescence test
In vitro study
Plasmodium falciparum
Preschool child
Protein binding
Protein domain
Protein motif
Amino acid motifs
Erythrocytes
Immunoglobulin g
Molecular sequence data
Plasmodium falciparum
Plasmodium falciparum
- Rights
- License
- Abierto (Texto completo)
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Repositorio EdocUR - U. Rosario |
repository_id_str |
|
dc.title.spa.fl_str_mv |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
spellingShingle |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies Malaria Inmunología PfEMP1 Plasmodium Erythrocyte membrane protein 1 protozoan Protein antibody Molecular Preschool Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Child Disease severity Erythrocyte Flow cytometry Immunofluorescence test In vitro study Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Erythrocytes Immunoglobulin g Molecular sequence data Plasmodium falciparum Plasmodium falciparum |
title_short |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_full |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_fullStr |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_full_unstemmed |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_sort |
A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
dc.subject.decs.spa.fl_str_mv |
Malaria Inmunología PfEMP1 Plasmodium |
topic |
Malaria Inmunología PfEMP1 Plasmodium Erythrocyte membrane protein 1 protozoan Protein antibody Molecular Preschool Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Child Disease severity Erythrocyte Flow cytometry Immunofluorescence test In vitro study Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Erythrocytes Immunoglobulin g Molecular sequence data Plasmodium falciparum Plasmodium falciparum |
dc.subject.keyword.eng.fl_str_mv |
Erythrocyte membrane protein 1 protozoan Protein antibody Molecular Preschool Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Child Disease severity Erythrocyte Flow cytometry Immunofluorescence test In vitro study Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Erythrocytes Immunoglobulin g Molecular sequence data Plasmodium falciparum Plasmodium falciparum |
description |
Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface |
publishDate |
2013 |
dc.date.created.none.fl_str_mv |
2013-01-15 |
dc.date.issued.none.fl_str_mv |
2013 |
dc.date.accessioned.none.fl_str_mv |
2014-08-11T14:48:06Z |
dc.date.available.none.fl_str_mv |
2014-08-11T14:48:06Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1371/journal.pone.0052679 |
dc.identifier.issn.none.fl_str_mv |
1932-6203 |
dc.identifier.uri.none.fl_str_mv |
http://repository.urosario.edu.co/handle/10336/8765 |
url |
https://doi.org/10.1371/journal.pone.0052679 http://repository.urosario.edu.co/handle/10336/8765 |
identifier_str_mv |
1932-6203 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationTitle.none.fl_str_mv |
PLOS ONE |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 8 |
dc.relation.ispartof.spa.fl_str_mv |
PLOS ONE, ISSN 1932-6203, V. 8 N. 1 Jan, 2013 |
dc.relation.uri.none.fl_str_mv |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052679 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto completo) |
rights_invalid_str_mv |
Abierto (Texto completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.medium.spa.fl_str_mv |
Recurso electrónico |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.format.tipo.spa.fl_str_mv |
Documento |
dc.publisher.spa.fl_str_mv |
Universidad del Rosario |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
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Comunidad Rosarista4337b0a3-0238-4c14-bf8b-8b98d2e9908b6000f56ff8d-4ec1-4d07-a27e-c0e6e805eee660078b2f455-da24-4179-9e9b-cf47f469384a6007333b7bb-1950-4186-9858-b8b1a9896af26004d5e3094-2e0c-422e-a290-a7c176a03875600ab65f82c-df13-44e4-9b71-f3d272750d78600c80d29d7-0466-42aa-b3ea-248382006932600f007bd51-2df9-4895-ba8e-2fb0080c5a4e6002014-08-11T14:48:06Z2014-08-11T14:48:06Z2013-01-152013Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surfaceRecurso electrónicoapplication/pdfDocumentohttps://doi.org/10.1371/journal.pone.00526791932-6203http://repository.urosario.edu.co/handle/10336/8765engUniversidad del RosarioNo. 1PLOS ONEVol. 8PLOS ONE, ISSN 1932-6203, V. 8 N. 1 Jan, 2013https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052679Abierto (Texto completo)EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMalariaInmunologíaPfEMP1PlasmodiumErythrocyte membrane protein 1protozoanProtein antibodyMolecularPreschoolStrain transcending antibodyUnclassified drugAmino acid sequenceAntigen antibody reactionAntigen bindingChildDisease severityErythrocyteFlow cytometryImmunofluorescence testIn vitro studyPlasmodium falciparumPreschool childProtein bindingProtein domainProtein motifAmino acid motifsErythrocytesImmunoglobulin gMolecular sequence dataPlasmodium falciparumPlasmodium falciparumA Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending AntibodiesarticleArtículohttp://purl.org/coar/resource_type/c_6501Blomqvist, KarinAlbrecht, LetusaQuintana, Maria del PilarAngeletti, DavideJoannin, NicolasChêne, ArnaudMoll, KirstenWahlgren, MatsBlomqvist, KarinAlbrecht, LetusaQuintana, Maria del PilarAngeletti, DavideJoannin, NicolasChêne, ArnaudMoll, KirstenWahlgren, MatsORIGINALA Sequence in Subdomain 2 of DBL1a.pdfA Sequence in Subdomain 2 of DBL1a.pdfapplication/pdf1134717https://repository.urosario.edu.co/bitstreams/ee94c6bb-ee01-4074-a7ea-223adc102bd8/download4edbfa625c0a482702b50a09187490daMD51LICENSElicense.txtlicense.txttext/plain2156https://repository.urosario.edu.co/bitstreams/99936216-b115-4402-977d-679cb81959a4/downloadb4f8fe66e94b897ab4c355bac005ad16MD52TEXTA Sequence in Subdomain 2 of DBL1a.pdf.txtA Sequence in Subdomain 2 of DBL1a.pdf.txtExtracted texttext/plain61756https://repository.urosario.edu.co/bitstreams/dbb995d1-a0f4-4b37-9790-b1763cdfee27/downloadb62ae89570acac99c1297417715308b1MD59THUMBNAILA Sequence in Subdomain 2 of DBL1a.pdf.jpgA Sequence in Subdomain 2 of DBL1a.pdf.jpgGenerated Thumbnailimage/jpeg4909https://repository.urosario.edu.co/bitstreams/0d2e4d27-4c0e-41a0-bd4b-621c222e4e34/downloadb9ea5aa55f7d8910139cd04ed01512f5MD51010336/8765oai:repository.urosario.edu.co:10336/87652022-08-31 12:44:56.677https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.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