A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies
Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestrati...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2013
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/22840
- Acceso en línea:
- https://doi.org/10.1371/journal.pone.0052679
https://repository.urosario.edu.co/handle/10336/22840
- Palabra clave:
- Erythrocyte membrane protein 1
Protein antibody
Strain transcending antibody
Unclassified drug
Amino acid sequence
Antigen antibody reaction
Antigen binding
Article
Child
Disease severity
Erythrocyte
Female
Flow cytometry
Human
Immunofluorescence test
In vitro study
Malaria
Male
Nonhuman
Plasmodium falciparum
Preschool child
Protein binding
Protein domain
Protein motif
Amino acid motifs
Amino acid sequence
Animals
Antibody specificity
Cross reactions
Epitopes
Erythrocytes
Female
Humans
Immunoglobulin g
Infant
Male
Molecular sequence data
Peptides
Plasmodium falciparum
Protein binding
Protein conformation
Protein interaction domains and motifs
Protozoan proteins
Rabbits
Rats
Plasmodium falciparum
molecular
protozoan
protozoan
preschool
Antibodies
Antigens
Child
Models
- Rights
- License
- Abierto (Texto Completo)
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dc.title.spa.fl_str_mv |
A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title |
A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
spellingShingle |
A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies Erythrocyte membrane protein 1 Protein antibody Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Article Child Disease severity Erythrocyte Female Flow cytometry Human Immunofluorescence test In vitro study Malaria Male Nonhuman Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Amino acid sequence Animals Antibody specificity Cross reactions Epitopes Erythrocytes Female Humans Immunoglobulin g Infant Male Molecular sequence data Peptides Plasmodium falciparum Protein binding Protein conformation Protein interaction domains and motifs Protozoan proteins Rabbits Rats Plasmodium falciparum molecular protozoan protozoan preschool Antibodies Antigens Child Models |
title_short |
A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_full |
A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_fullStr |
A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_full_unstemmed |
A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
title_sort |
A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies |
dc.subject.keyword.spa.fl_str_mv |
Erythrocyte membrane protein 1 Protein antibody Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Article Child Disease severity Erythrocyte Female Flow cytometry Human Immunofluorescence test In vitro study Malaria Male Nonhuman Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Amino acid sequence Animals Antibody specificity Cross reactions Epitopes Erythrocytes Female Humans Immunoglobulin g Infant Male Molecular sequence data Peptides Plasmodium falciparum Protein binding Protein conformation Protein interaction domains and motifs Protozoan proteins Rabbits Rats Plasmodium falciparum |
topic |
Erythrocyte membrane protein 1 Protein antibody Strain transcending antibody Unclassified drug Amino acid sequence Antigen antibody reaction Antigen binding Article Child Disease severity Erythrocyte Female Flow cytometry Human Immunofluorescence test In vitro study Malaria Male Nonhuman Plasmodium falciparum Preschool child Protein binding Protein domain Protein motif Amino acid motifs Amino acid sequence Animals Antibody specificity Cross reactions Epitopes Erythrocytes Female Humans Immunoglobulin g Infant Male Molecular sequence data Peptides Plasmodium falciparum Protein binding Protein conformation Protein interaction domains and motifs Protozoan proteins Rabbits Rats Plasmodium falciparum molecular protozoan protozoan preschool Antibodies Antigens Child Models |
dc.subject.keyword.eng.fl_str_mv |
molecular protozoan protozoan preschool Antibodies Antigens Child Models |
description |
Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1? previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1?-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1? antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface. © 2013 Blomqvist et al. |
publishDate |
2013 |
dc.date.created.spa.fl_str_mv |
2013 |
dc.date.accessioned.none.fl_str_mv |
2020-05-25T23:58:19Z |
dc.date.available.none.fl_str_mv |
2020-05-25T23:58:19Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1371/journal.pone.0052679 |
dc.identifier.issn.none.fl_str_mv |
19326203 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/22840 |
url |
https://doi.org/10.1371/journal.pone.0052679 https://repository.urosario.edu.co/handle/10336/22840 |
identifier_str_mv |
19326203 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationTitle.none.fl_str_mv |
PLoS ONE |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 8 |
dc.relation.ispartof.spa.fl_str_mv |
PLoS ONE, ISSN:19326203, Vol.8, No.1 (2013) |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872406445&doi=10.1371%2fjournal.pone.0052679&partnerID=40&md5=2d29dbe442d2130574d0921d247d2bd1 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
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Abierto (Texto Completo) |
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Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
institution |
Universidad del Rosario |
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reponame:Repositorio Institucional EdocUR |
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4337b0a3-0238-4c14-bf8b-8b98d2e9908b-10f56ff8d-4ec1-4d07-a27e-c0e6e805eee6-1a57d52c1-7383-4c4b-8924-876e82fd1697-17333b7bb-1950-4186-9858-b8b1a9896af2-14d5e3094-2e0c-422e-a290-a7c176a03875-1ab65f82c-df13-44e4-9b71-f3d272750d78-116847421-b258-4e84-809a-922065d4bf19-1f007bd51-2df9-4895-ba8e-2fb0080c5a4e-12020-05-25T23:58:19Z2020-05-25T23:58:19Z2013Immunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1? previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1?-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1? antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface. © 2013 Blomqvist et al.application/pdfhttps://doi.org/10.1371/journal.pone.005267919326203https://repository.urosario.edu.co/handle/10336/22840engNo. 1PLoS ONEVol. 8PLoS ONE, ISSN:19326203, Vol.8, No.1 (2013)https://www.scopus.com/inward/record.uri?eid=2-s2.0-84872406445&doi=10.1371%2fjournal.pone.0052679&partnerID=40&md5=2d29dbe442d2130574d0921d247d2bd1Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURErythrocyte membrane protein 1Protein antibodyStrain transcending antibodyUnclassified drugAmino acid sequenceAntigen antibody reactionAntigen bindingArticleChildDisease severityErythrocyteFemaleFlow cytometryHumanImmunofluorescence testIn vitro studyMalariaMaleNonhumanPlasmodium falciparumPreschool childProtein bindingProtein domainProtein motifAmino acid motifsAmino acid sequenceAnimalsAntibody specificityCross reactionsEpitopesErythrocytesFemaleHumansImmunoglobulin gInfantMaleMolecular sequence dataPeptidesPlasmodium falciparumProtein bindingProtein conformationProtein interaction domains and motifsProtozoan proteinsRabbitsRatsPlasmodium falciparummolecularprotozoanprotozoanpreschoolAntibodiesAntigensChildModelsA Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending AntibodiesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Blomqvist, KarinAlbrecht, Letusadel Pilar Quintana, MariaAngeletti, DavideJoannin, NicolasChêne, ArnaudMoll, KirstenWahlgren, MatsORIGINALjournal-pone-0052679.pdfapplication/pdf1134717https://repository.urosario.edu.co/bitstreams/b5cf90df-c691-4f3b-b784-ee03cbbe84f1/download4edbfa625c0a482702b50a09187490daMD51TEXTjournal-pone-0052679.pdf.txtjournal-pone-0052679.pdf.txtExtracted texttext/plain61756https://repository.urosario.edu.co/bitstreams/22118270-696b-4dd6-9caf-8cb655eceba7/downloadb62ae89570acac99c1297417715308b1MD52THUMBNAILjournal-pone-0052679.pdf.jpgjournal-pone-0052679.pdf.jpgGenerated Thumbnailimage/jpeg5054https://repository.urosario.edu.co/bitstreams/e281b76a-6275-4cc0-a085-e8c2847c2c61/downloada04d12f5208a6f5617ae372d08032832MD5310336/22840oai:repository.urosario.edu.co:10336/228402022-05-02 07:37:20.672581https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |