Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

Aim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to det...

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Autores:

Tipo de recurso:

Fecha de publicación:

2019

Institución:

Universidad del Rosario

Repositorio:

Repositorio EdocUR - U. Rosario

Idioma:

eng

OAI Identifier:

oai:repository.urosario.edu.co:10336/22126

Acceso en línea:

https://doi.org/10.1016/j.ijid.2018.10.029
https://repository.urosario.edu.co/handle/10336/22126

Palabra clave:

Protozoal DNA
Protozoal DNA
Article
Droplet digital polymerase chain reaction
Human
Leishmania
Leishmania braziliensis
Leishmania donovani
Leishmania guyanensis
Leishmania infantum
Leishmania major
Leishmania mexicana
Leishmania panamensis
Limit of detection
Microorganism detection
Molecular pathology
Skin leishmaniasis
Task performance
Validation study
Evaluation study
Female
Genetics
Isolation and purification
Leishmania
Parasitology
Procedures
Real time polymerase chain reaction
Sensitivity and specificity
Skin leishmaniasis
Female
Humans
Leishmania
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Cutaneous Leishmaniasis
Ddpcr
Leishmania
Molecular diagnosis
Cutaneous
Protozoan
DNA
Leishmaniasis

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Abierto (Texto Completo)