High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals

Vitamin D (VitD) is an endogenous immunomodulator that could protect from HIV-1 infection reducing immune activation and inducing the expression of anti-HIV-1 peptides. To establish a correlation between VitD and natural resistance to HIV-1 infection, a case-control study using blood and mucosa samp...

Full description

Autores:
Aguilar-Jiménez W.
Zapata Builes, Wildeman
Caruz A.
Rugeles M.T.
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/41889
Acceso en línea:
https://doi.org/10.17533/udea.iatreia.v29n4a06
https://revistas.ucr.ac.cr/index.php/aie/article/view/28679
https://hdl.handle.net/20.500.12494/41889
Palabra clave:
beta defensin 2
beta defensin 3
interleukin 10
interleukin 1beta
messenger RNA
transforming growth factor beta
tumor necrosis factor alpha
vitamin D receptor
adult
article
case control study
controlled study
cross-sectional study
enzyme linked immunosorbent assay
exposure
female
gene expression
genital mucosa
human
Human immunodeficiency virus 1 infection
human tissue
major clinical study
male
mouth mucosa
mucosa
peripheral blood mononuclear cell
reverse transcription polymerase chain reaction
upregulation
vitamin blood level
Adult
beta-Defensins
Cross-Sectional Studies
Cytokines
Disease Resistance
Female
HIV Infections
HIV Seronegativity
HIV-1
Humans
Interleukin-10
Male
Mouth Mucosa
Mucous Membrane
Receptors
Calcitriol
RNA
Messenger
Transcription
Genetic
Vitamin D
Young Adult
Rights
closedAccess
License
http://purl.org/coar/access_right/c_14cb
id COOPER2_fa761a01990187a08126cc9fec5b5841
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/41889
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Aguilar-Jiménez W.Zapata Builes, WildemanCaruz A.Rugeles M.T.2021-12-16T22:15:52Z2021-12-16T22:15:52Z2013https://doi.org/10.17533/udea.iatreia.v29n4a06https://revistas.ucr.ac.cr/index.php/aie/article/view/2867919326203https://hdl.handle.net/20.500.12494/41889Aguilar W,Zapata W,Caruz A,Rugeles MT. High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals. PLoS One. 2013. 8. (12):e82717. .Vitamin D (VitD) is an endogenous immunomodulator that could protect from HIV-1 infection reducing immune activation and inducing the expression of anti-HIV-1 peptides. To establish a correlation between VitD and natural resistance to HIV-1 infection, a case-control study using blood and mucosa samples of 58 HIV-1-exposed but seronegative (HESN) individuals, 43 HIV-1 seropositives (SPs) and 59 non-exposed healthy controls (HCs) was carried out. The VitD concentration in plasma was determined by ELISA, and mRNA relative units (RU) of VDR, IL-10, TGF-ß, TNF-a and IL-1ß in peripheral blood mononuclear cells (PBMCs), oral and genital mucosa was quantified by qRT-PCR. mRNA levels of human beta-defensin (HBD) -2 and -3 were previously reported and used for correlations. Significantly higher levels of VitD were found in plasma as well as higher mRNA RU of VDR in PBMCs, and in genital mucosa from HESN compared to HCs. In addition, higher mRNA RU of TNF-a, IL-1ß and IL-10, and lower mRNA RU of TGF-ß were found in PBMC from HESNs compared to HCs. We also observed higher IL-10 mRNA RU in genital mucosa of HESNs compared to HCs, and the mRNA levels of TNF-a in oral and genital mucosa of SPs were higher compared to HESNs. Furthermore, positive correlations between VDR and IL-10 mRNA RU in PBMCs and genital mucosa of HESNs were found. Finally, HBD-2 and HBD-3 mRNA RU were positively correlated with VDR mRNA expression in oral mucosa from HESNs. These results suggest that high levels of VitD and its receptor are associated with natural resistance to HIV-1 infection. Up-regulation of the anti-inflammatory IL-10, and the induction of anti-HIV-1 defensins in mucosa might be part of the mechanisms involved in this association. However, further studies are required to define causal associations. Copyright: © 2013 Aguilar-Jiménez et al.0000-0002-7351-8738wildeman.zapatab@campusucc.edu.co-Public Library of Sciencebeta defensin 2beta defensin 3interleukin 10interleukin 1betamessenger RNAtransforming growth factor betatumor necrosis factor alphavitamin D receptoradultarticlecase control studycontrolled studycross-sectional studyenzyme linked immunosorbent assayexposurefemalegene expressiongenital mucosahumanHuman immunodeficiency virus 1 infectionhuman tissuemajor clinical studymalemouth mucosamucosaperipheral blood mononuclear cellreverse transcription polymerase chain reactionupregulationvitamin blood levelAdultbeta-DefensinsCross-Sectional StudiesCytokinesDisease ResistanceFemaleHIV InfectionsHIV SeronegativityHIV-1HumansInterleukin-10MaleMouth MucosaMucous MembraneReceptorsCalcitriolRNAMessengerTranscriptionGeneticVitamin DYoung AdultHigh transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individualsArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPlos Oneinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41889oai:repository.ucc.edu.co:20.500.12494/418892024-08-20 16:23:05.218metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals
title High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals
spellingShingle High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals
beta defensin 2
beta defensin 3
interleukin 10
interleukin 1beta
messenger RNA
transforming growth factor beta
tumor necrosis factor alpha
vitamin D receptor
adult
article
case control study
controlled study
cross-sectional study
enzyme linked immunosorbent assay
exposure
female
gene expression
genital mucosa
human
Human immunodeficiency virus 1 infection
human tissue
major clinical study
male
mouth mucosa
mucosa
peripheral blood mononuclear cell
reverse transcription polymerase chain reaction
upregulation
vitamin blood level
Adult
beta-Defensins
Cross-Sectional Studies
Cytokines
Disease Resistance
Female
HIV Infections
HIV Seronegativity
HIV-1
Humans
Interleukin-10
Male
Mouth Mucosa
Mucous Membrane
Receptors
Calcitriol
RNA
Messenger
Transcription
Genetic
Vitamin D
Young Adult
title_short High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals
title_full High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals
title_fullStr High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals
title_full_unstemmed High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals
title_sort High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals
dc.creator.fl_str_mv Aguilar-Jiménez W.
Zapata Builes, Wildeman
Caruz A.
Rugeles M.T.
dc.contributor.author.none.fl_str_mv Aguilar-Jiménez W.
Zapata Builes, Wildeman
Caruz A.
Rugeles M.T.
dc.subject.spa.fl_str_mv beta defensin 2
beta defensin 3
interleukin 10
interleukin 1beta
messenger RNA
transforming growth factor beta
tumor necrosis factor alpha
vitamin D receptor
adult
article
case control study
controlled study
cross-sectional study
enzyme linked immunosorbent assay
exposure
female
gene expression
genital mucosa
human
Human immunodeficiency virus 1 infection
human tissue
major clinical study
male
mouth mucosa
mucosa
peripheral blood mononuclear cell
reverse transcription polymerase chain reaction
upregulation
vitamin blood level
Adult
beta-Defensins
Cross-Sectional Studies
Cytokines
Disease Resistance
Female
HIV Infections
HIV Seronegativity
HIV-1
Humans
Interleukin-10
Male
Mouth Mucosa
Mucous Membrane
Receptors
Calcitriol
RNA
Messenger
Transcription
Genetic
Vitamin D
Young Adult
topic beta defensin 2
beta defensin 3
interleukin 10
interleukin 1beta
messenger RNA
transforming growth factor beta
tumor necrosis factor alpha
vitamin D receptor
adult
article
case control study
controlled study
cross-sectional study
enzyme linked immunosorbent assay
exposure
female
gene expression
genital mucosa
human
Human immunodeficiency virus 1 infection
human tissue
major clinical study
male
mouth mucosa
mucosa
peripheral blood mononuclear cell
reverse transcription polymerase chain reaction
upregulation
vitamin blood level
Adult
beta-Defensins
Cross-Sectional Studies
Cytokines
Disease Resistance
Female
HIV Infections
HIV Seronegativity
HIV-1
Humans
Interleukin-10
Male
Mouth Mucosa
Mucous Membrane
Receptors
Calcitriol
RNA
Messenger
Transcription
Genetic
Vitamin D
Young Adult
description Vitamin D (VitD) is an endogenous immunomodulator that could protect from HIV-1 infection reducing immune activation and inducing the expression of anti-HIV-1 peptides. To establish a correlation between VitD and natural resistance to HIV-1 infection, a case-control study using blood and mucosa samples of 58 HIV-1-exposed but seronegative (HESN) individuals, 43 HIV-1 seropositives (SPs) and 59 non-exposed healthy controls (HCs) was carried out. The VitD concentration in plasma was determined by ELISA, and mRNA relative units (RU) of VDR, IL-10, TGF-ß, TNF-a and IL-1ß in peripheral blood mononuclear cells (PBMCs), oral and genital mucosa was quantified by qRT-PCR. mRNA levels of human beta-defensin (HBD) -2 and -3 were previously reported and used for correlations. Significantly higher levels of VitD were found in plasma as well as higher mRNA RU of VDR in PBMCs, and in genital mucosa from HESN compared to HCs. In addition, higher mRNA RU of TNF-a, IL-1ß and IL-10, and lower mRNA RU of TGF-ß were found in PBMC from HESNs compared to HCs. We also observed higher IL-10 mRNA RU in genital mucosa of HESNs compared to HCs, and the mRNA levels of TNF-a in oral and genital mucosa of SPs were higher compared to HESNs. Furthermore, positive correlations between VDR and IL-10 mRNA RU in PBMCs and genital mucosa of HESNs were found. Finally, HBD-2 and HBD-3 mRNA RU were positively correlated with VDR mRNA expression in oral mucosa from HESNs. These results suggest that high levels of VitD and its receptor are associated with natural resistance to HIV-1 infection. Up-regulation of the anti-inflammatory IL-10, and the induction of anti-HIV-1 defensins in mucosa might be part of the mechanisms involved in this association. However, further studies are required to define causal associations. Copyright: © 2013 Aguilar-Jiménez et al.
publishDate 2013
dc.date.issued.none.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:15:52Z
dc.date.available.none.fl_str_mv 2021-12-16T22:15:52Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.17533/udea.iatreia.v29n4a06
https://revistas.ucr.ac.cr/index.php/aie/article/view/28679
dc.identifier.issn.spa.fl_str_mv 19326203
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/41889
dc.identifier.bibliographicCitation.spa.fl_str_mv Aguilar W,Zapata W,Caruz A,Rugeles MT. High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals. PLoS One. 2013. 8. (12):e82717. .
url https://doi.org/10.17533/udea.iatreia.v29n4a06
https://revistas.ucr.ac.cr/index.php/aie/article/view/28679
https://hdl.handle.net/20.500.12494/41889
identifier_str_mv 19326203
Aguilar W,Zapata W,Caruz A,Rugeles MT. High transcript levels of vitamin D receptor are correlated with higher mRNA expression of human beta defensins and IL-10 in Mucosa of HIV-1-exposed seronegative individuals. PLoS One. 2013. 8. (12):e82717. .
dc.relation.ispartofjournal.spa.fl_str_mv Plos One
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv -
dc.publisher.spa.fl_str_mv Public Library of Science
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
_version_ 1814247319993843712