Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.

Alternative splicing as a mean to control gene expression and diversify function is suspected to considerably influence drug response and clearance. We report the quantitative expression profiles of the human UGT genes including alternatively spliced variants not previously annotated established by...

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Autores:
Tourancheau A
Rouleau M
Guauque Olarte, Sandra milena
Villeneuve L
Gilbert I
Droit A
Guillemette C
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/50266
Acceso en línea:
https://doi.org/10.1038/tpj.2017.5
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046073558&doi=10.1038%2ftpj.2017.5&partnerID=40&md5=e81a249eafd5030d4af5d88884bf09c7
https://hdl.handle.net/20.500.12494/50266
Palabra clave:
ALTERNATIVE RNA SPLICING
ARTICLE
CARCINOGENESIS
CONTROLLED STUDY
DRUG
DRUG CLEARANCE
DRUG EFFECT
DRUG METABOLISM
DRUG RESPONSE
FEMALE
GENE
GENE EXPRESSION PROFILING
GENE EXPRESSION REGULATION
GENETIC VARIABILITY
GENETICS
GLUCURONOSYLTRANSFERASE
HUMAN
HUMAN TISSUE
HUMANS
INTERINDIVIDUAL VARIABILITY
MALE
METABOLIC CLEARANCE RATE
METABOLISM
PHARMACEUTICAL PREPARATIONS
PHARMACOGENETIC VARIANT
PHARMACOGENOMIC VARIANTS
PHYSIOLOGY
PRIORITY JOURNAL
PROCEDURES
PROTEOME
RNA SEQUENCE
TISSUE DISTRIBUTION
TRANSCRIPTOME
UGT GENE
UGT1 GENE
UGT1A1 ENZYME
UGT2 GENE
UGT2A1 PROTEIN, HUMAN
Rights
openAccess
License
http://purl.org/coar/access_right/c_abf2
id COOPER2_e06169fa7dc30eda7f944b2436f7ff27
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/50266
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Tourancheau ARouleau MGuauque Olarte, Sandra milenaVilleneuve LGilbert IDroit AGuillemette C2023-05-24T16:26:00Z2023-05-24T16:26:00Z25/04/2018https://doi.org/10.1038/tpj.2017.5https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046073558&doi=10.1038%2ftpj.2017.5&partnerID=40&md5=e81a249eafd5030d4af5d88884bf09c71470269Xhttps://hdl.handle.net/20.500.12494/50266Tourancheau A,Rouleau M,Guauque Olarte Sandra milena,Villeneuve L,Gilbert I,Droit A,Guillemette C.Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues..PHARMACOGENOMICS JOURNAL. 2018. 18. (2):p. 251-261Alternative splicing as a mean to control gene expression and diversify function is suspected to considerably influence drug response and clearance. We report the quantitative expression profiles of the human UGT genes including alternatively spliced variants not previously annotated established by deep RNA-sequencing in tissues of pharmacological importance. We reveal a comprehensive quantification of the alternative UGT transcriptome that differ across tissues and among individuals. Alternative transcripts that comprise novel in-frame sequences associated or not with truncations of the 5'- and/or 3'- termini, significantly contribute to the total expression levels of each UGT1 and UGT2 gene averaging 21% in normal tissues, with expression of UGT2 variants surpassing those of UGT1. Quantitative data expose preferential tissue expression patterns and remodeling in favor of alternative variants upon tumorigenesis. These complex alternative splicing programs have the strong potential to contribute to interindividual variability in drug metabolism in addition to diversify the UGT proteome.0000-0003-0336-9682sandra.guauque@campusucc.edu.co251-261Nature Publishing GroupALTERNATIVE RNA SPLICINGARTICLECARCINOGENESISCONTROLLED STUDYDRUGDRUG CLEARANCEDRUG EFFECTDRUG METABOLISMDRUG RESPONSEFEMALEGENEGENE EXPRESSION PROFILINGGENE EXPRESSION REGULATIONGENETIC VARIABILITYGENETICSGLUCURONOSYLTRANSFERASEHUMANHUMAN TISSUEHUMANSINTERINDIVIDUAL VARIABILITYMALEMETABOLIC CLEARANCE RATEMETABOLISMPHARMACEUTICAL PREPARATIONSPHARMACOGENETIC VARIANTPHARMACOGENOMIC VARIANTSPHYSIOLOGYPRIORITY JOURNALPROCEDURESPROTEOMERNA SEQUENCETISSUE DISTRIBUTIONTRANSCRIPTOMEUGT GENEUGT1 GENEUGT1A1 ENZYMEUGT2 GENEUGT2A1 PROTEIN, HUMANQuantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPHARMACOGENOMICS JOURNALinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Publication20.500.12494/50266oai:repository.ucc.edu.co:20.500.12494/502662024-08-20 16:18:22.498metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
spellingShingle Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
ALTERNATIVE RNA SPLICING
ARTICLE
CARCINOGENESIS
CONTROLLED STUDY
DRUG
DRUG CLEARANCE
DRUG EFFECT
DRUG METABOLISM
DRUG RESPONSE
FEMALE
GENE
GENE EXPRESSION PROFILING
GENE EXPRESSION REGULATION
GENETIC VARIABILITY
GENETICS
GLUCURONOSYLTRANSFERASE
HUMAN
HUMAN TISSUE
HUMANS
INTERINDIVIDUAL VARIABILITY
MALE
METABOLIC CLEARANCE RATE
METABOLISM
PHARMACEUTICAL PREPARATIONS
PHARMACOGENETIC VARIANT
PHARMACOGENOMIC VARIANTS
PHYSIOLOGY
PRIORITY JOURNAL
PROCEDURES
PROTEOME
RNA SEQUENCE
TISSUE DISTRIBUTION
TRANSCRIPTOME
UGT GENE
UGT1 GENE
UGT1A1 ENZYME
UGT2 GENE
UGT2A1 PROTEIN, HUMAN
title_short Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title_full Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title_fullStr Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title_full_unstemmed Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title_sort Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
dc.creator.fl_str_mv Tourancheau A
Rouleau M
Guauque Olarte, Sandra milena
Villeneuve L
Gilbert I
Droit A
Guillemette C
dc.contributor.author.none.fl_str_mv Tourancheau A
Rouleau M
Guauque Olarte, Sandra milena
Villeneuve L
Gilbert I
Droit A
Guillemette C
dc.subject.spa.fl_str_mv ALTERNATIVE RNA SPLICING
ARTICLE
CARCINOGENESIS
CONTROLLED STUDY
DRUG
DRUG CLEARANCE
DRUG EFFECT
DRUG METABOLISM
DRUG RESPONSE
FEMALE
GENE
GENE EXPRESSION PROFILING
GENE EXPRESSION REGULATION
GENETIC VARIABILITY
GENETICS
GLUCURONOSYLTRANSFERASE
HUMAN
HUMAN TISSUE
HUMANS
INTERINDIVIDUAL VARIABILITY
MALE
METABOLIC CLEARANCE RATE
METABOLISM
PHARMACEUTICAL PREPARATIONS
PHARMACOGENETIC VARIANT
PHARMACOGENOMIC VARIANTS
PHYSIOLOGY
PRIORITY JOURNAL
PROCEDURES
PROTEOME
RNA SEQUENCE
TISSUE DISTRIBUTION
TRANSCRIPTOME
UGT GENE
UGT1 GENE
UGT1A1 ENZYME
UGT2 GENE
UGT2A1 PROTEIN, HUMAN
topic ALTERNATIVE RNA SPLICING
ARTICLE
CARCINOGENESIS
CONTROLLED STUDY
DRUG
DRUG CLEARANCE
DRUG EFFECT
DRUG METABOLISM
DRUG RESPONSE
FEMALE
GENE
GENE EXPRESSION PROFILING
GENE EXPRESSION REGULATION
GENETIC VARIABILITY
GENETICS
GLUCURONOSYLTRANSFERASE
HUMAN
HUMAN TISSUE
HUMANS
INTERINDIVIDUAL VARIABILITY
MALE
METABOLIC CLEARANCE RATE
METABOLISM
PHARMACEUTICAL PREPARATIONS
PHARMACOGENETIC VARIANT
PHARMACOGENOMIC VARIANTS
PHYSIOLOGY
PRIORITY JOURNAL
PROCEDURES
PROTEOME
RNA SEQUENCE
TISSUE DISTRIBUTION
TRANSCRIPTOME
UGT GENE
UGT1 GENE
UGT1A1 ENZYME
UGT2 GENE
UGT2A1 PROTEIN, HUMAN
description Alternative splicing as a mean to control gene expression and diversify function is suspected to considerably influence drug response and clearance. We report the quantitative expression profiles of the human UGT genes including alternatively spliced variants not previously annotated established by deep RNA-sequencing in tissues of pharmacological importance. We reveal a comprehensive quantification of the alternative UGT transcriptome that differ across tissues and among individuals. Alternative transcripts that comprise novel in-frame sequences associated or not with truncations of the 5'- and/or 3'- termini, significantly contribute to the total expression levels of each UGT1 and UGT2 gene averaging 21% in normal tissues, with expression of UGT2 variants surpassing those of UGT1. Quantitative data expose preferential tissue expression patterns and remodeling in favor of alternative variants upon tumorigenesis. These complex alternative splicing programs have the strong potential to contribute to interindividual variability in drug metabolism in addition to diversify the UGT proteome.
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-05-24T16:26:00Z
dc.date.available.none.fl_str_mv 2023-05-24T16:26:00Z
dc.date.issued.none.fl_str_mv 25/04/2018
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1038/tpj.2017.5
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046073558&doi=10.1038%2ftpj.2017.5&partnerID=40&md5=e81a249eafd5030d4af5d88884bf09c7
dc.identifier.issn.spa.fl_str_mv 1470269X
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/50266
dc.identifier.bibliographicCitation.spa.fl_str_mv Tourancheau A,Rouleau M,Guauque Olarte Sandra milena,Villeneuve L,Gilbert I,Droit A,Guillemette C.Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues..PHARMACOGENOMICS JOURNAL. 2018. 18. (2):p. 251-261
url https://doi.org/10.1038/tpj.2017.5
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046073558&doi=10.1038%2ftpj.2017.5&partnerID=40&md5=e81a249eafd5030d4af5d88884bf09c7
https://hdl.handle.net/20.500.12494/50266
identifier_str_mv 1470269X
Tourancheau A,Rouleau M,Guauque Olarte Sandra milena,Villeneuve L,Gilbert I,Droit A,Guillemette C.Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues..PHARMACOGENOMICS JOURNAL. 2018. 18. (2):p. 251-261
dc.relation.ispartofjournal.spa.fl_str_mv PHARMACOGENOMICS JOURNAL
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 251-261
dc.publisher.spa.fl_str_mv Nature Publishing Group
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
_version_ 1814246857725968384

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