HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro
Toll-like receptors (TLRs) play a critical role in innate immunity against pathogens. Their stimulation induces the activation of NF-?B, an important inducer of HIV-1 replication. In recent years, an increasing number of studies using several cells types from HIV-infected patients indicate that TLRs...
- Autores:
-
Hernández López, Juan Carlos
Stevenson M.
Latz E.
Urcuqui-Inchima S.
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2012
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/41726
- Acceso en línea:
- https://doi.org/10.15446/anpol.v31n94.78239
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053407586&doi=10.7203%2fCIRIEC-E.93.10327&partnerID=40&md5=bf4e34a3b306679b0594288956a8839c
https://hdl.handle.net/20.500.12494/41726
- Palabra clave:
- abacavir
amprenavir
B7 antigen
beta actin
CD123 antigen
CD14 antigen
CD4 antigen
cytokine
didanosine
efavirenz
fosamprenavir
immunoglobulin enhancer binding protein
interleukin 1beta
interleukin 6
lamivudine
lopinavir
messenger RNA
nelfinavir
nevirapine
saquinavir
stavudine
toll like receptor 2
toll like receptor 4
tumor necrosis factor alpha
zidovudine
acquired immune deficiency syndrome
adolescent
adult
article
CD4+ T lymphocyte
cell subpopulation
clinical article
controlled study
cytokine production
ex vivo study
female
gene expression regulation
highly active antiretroviral therapy
human
human cell
Human immunodeficiency virus 1
Human immunodeficiency virus 1 infection
Human immunodeficiency virus infected patient
immune response
immunomodulation
in vitro study
in vivo study
inflammation
innate immunity
macrophage
male
monocyte
myeloid dendritic cell
nonhuman
peripheral blood mononuclear cell
plasmacytoid dendritic cell
priori
- Rights
- closedAccess
- License
- http://purl.org/coar/access_right/c_14cb
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dc.title.spa.fl_str_mv |
HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro |
title |
HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro |
spellingShingle |
HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro abacavir amprenavir B7 antigen beta actin CD123 antigen CD14 antigen CD4 antigen cytokine didanosine efavirenz fosamprenavir immunoglobulin enhancer binding protein interleukin 1beta interleukin 6 lamivudine lopinavir messenger RNA nelfinavir nevirapine saquinavir stavudine toll like receptor 2 toll like receptor 4 tumor necrosis factor alpha zidovudine acquired immune deficiency syndrome adolescent adult article CD4+ T lymphocyte cell subpopulation clinical article controlled study cytokine production ex vivo study female gene expression regulation highly active antiretroviral therapy human human cell Human immunodeficiency virus 1 Human immunodeficiency virus 1 infection Human immunodeficiency virus infected patient immune response immunomodulation in vitro study in vivo study inflammation innate immunity macrophage male monocyte myeloid dendritic cell nonhuman peripheral blood mononuclear cell plasmacytoid dendritic cell priori |
title_short |
HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro |
title_full |
HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro |
title_fullStr |
HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro |
title_full_unstemmed |
HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro |
title_sort |
HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro |
dc.creator.fl_str_mv |
Hernández López, Juan Carlos Stevenson M. Latz E. Urcuqui-Inchima S. |
dc.contributor.author.none.fl_str_mv |
Hernández López, Juan Carlos Stevenson M. Latz E. Urcuqui-Inchima S. |
dc.subject.spa.fl_str_mv |
abacavir amprenavir B7 antigen beta actin CD123 antigen CD14 antigen CD4 antigen cytokine didanosine efavirenz fosamprenavir immunoglobulin enhancer binding protein interleukin 1beta interleukin 6 lamivudine lopinavir messenger RNA nelfinavir nevirapine saquinavir stavudine toll like receptor 2 toll like receptor 4 tumor necrosis factor alpha zidovudine acquired immune deficiency syndrome adolescent adult article CD4+ T lymphocyte cell subpopulation clinical article controlled study cytokine production ex vivo study female gene expression regulation highly active antiretroviral therapy human human cell Human immunodeficiency virus 1 Human immunodeficiency virus 1 infection Human immunodeficiency virus infected patient immune response immunomodulation in vitro study in vivo study inflammation innate immunity macrophage male monocyte myeloid dendritic cell nonhuman peripheral blood mononuclear cell plasmacytoid dendritic cell priori |
topic |
abacavir amprenavir B7 antigen beta actin CD123 antigen CD14 antigen CD4 antigen cytokine didanosine efavirenz fosamprenavir immunoglobulin enhancer binding protein interleukin 1beta interleukin 6 lamivudine lopinavir messenger RNA nelfinavir nevirapine saquinavir stavudine toll like receptor 2 toll like receptor 4 tumor necrosis factor alpha zidovudine acquired immune deficiency syndrome adolescent adult article CD4+ T lymphocyte cell subpopulation clinical article controlled study cytokine production ex vivo study female gene expression regulation highly active antiretroviral therapy human human cell Human immunodeficiency virus 1 Human immunodeficiency virus 1 infection Human immunodeficiency virus infected patient immune response immunomodulation in vitro study in vivo study inflammation innate immunity macrophage male monocyte myeloid dendritic cell nonhuman peripheral blood mononuclear cell plasmacytoid dendritic cell priori |
description |
Toll-like receptors (TLRs) play a critical role in innate immunity against pathogens. Their stimulation induces the activation of NF-?B, an important inducer of HIV-1 replication. In recent years, an increasing number of studies using several cells types from HIV-infected patients indicate that TLRs play a key role in regulating the expression of proinflammatory cytokines and viral pathogenesis. In the present study, the effect of HIV-1 stimulation of monocyte-derived macrophage (MDM) and peripheral blood mononuclear cell (PBMC) subpopulations from healthy donors on the expression and functions of TLR2 and TLR4 was examined. In addition, and to complete the in vitro study, the expression pattern of TLR2 and TLR4 in 49 HIV-1-infected patients, classified according to viral load and the use of HAART, was determined and compared with 25 healthy subjects. An increase of TLR expression and production of proinflammatory cytokines were observed in MDMs and PBMCs infected with HIV-1 in vitro and in response to TLR stimulation, compared to the mock. In addition, an association between TLR expression and up-regulation of CD80 in plasmacytoid dendritic cells (pDCs) was observed. The ex vivo analysis indicated increased expression of TLR2 and TLR4 in myeloid dendritic cells (mDCs), but only of TLR2 in monocytes obtained from HIV-1-infected patients, compared to healthy subjects. Remarkably, the expression was higher in cells from patients who do not use HAART. In monocytes, there was a positive correlation between both TLRs and viral load, but not CD4+ T cell numbers. Together, our in vitro and ex vivo results suggest that TLR expression and function can be up-regulated in response to HIV-1 infection and could affect the inflammatory response. We propose that modulation of TLRs represents a mechanism to promote HIV-1 replication or AIDS progression in HIV-1-infected patients. © 2012, Mary Ann Liebert, Inc. |
publishDate |
2012 |
dc.date.issued.none.fl_str_mv |
2012 |
dc.date.accessioned.none.fl_str_mv |
2021-12-16T22:15:44Z |
dc.date.available.none.fl_str_mv |
2021-12-16T22:15:44Z |
dc.type.none.fl_str_mv |
Artículo |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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http://purl.org/redcol/resource_type/ART |
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dc.identifier.none.fl_str_mv |
https://doi.org/10.15446/anpol.v31n94.78239 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053407586&doi=10.7203%2fCIRIEC-E.93.10327&partnerID=40&md5=bf4e34a3b306679b0594288956a8839c |
dc.identifier.issn.spa.fl_str_mv |
08892229 |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12494/41726 |
dc.identifier.bibliographicCitation.spa.fl_str_mv |
Hernández JC,Stevenson M,Latz E,Urcuqui S. HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro. AIDS Res Hum Retroviruses. 2012. 28. (10):p. 1313-1328. . |
url |
https://doi.org/10.15446/anpol.v31n94.78239 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053407586&doi=10.7203%2fCIRIEC-E.93.10327&partnerID=40&md5=bf4e34a3b306679b0594288956a8839c https://hdl.handle.net/20.500.12494/41726 |
identifier_str_mv |
08892229 Hernández JC,Stevenson M,Latz E,Urcuqui S. HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro. AIDS Res Hum Retroviruses. 2012. 28. (10):p. 1313-1328. . |
dc.relation.ispartofjournal.spa.fl_str_mv |
AIDS RES HUM RETROV |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/closedAccess |
dc.rights.coar.none.fl_str_mv |
http://purl.org/coar/access_right/c_14cb |
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closedAccess |
rights_invalid_str_mv |
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dc.format.extent.spa.fl_str_mv |
1328-1313 |
dc.publisher.spa.fl_str_mv |
Mary Ann Liebert Inc. |
institution |
Universidad Cooperativa de Colombia |
repository.name.fl_str_mv |
Repositorio Institucional Universidad Cooperativa de Colombia |
repository.mail.fl_str_mv |
bdigital@metabiblioteca.com |
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1814246852319510528 |
spelling |
Hernández López, Juan Carlos Stevenson M.Latz E.Urcuqui-Inchima S.2021-12-16T22:15:44Z2021-12-16T22:15:44Z2012https://doi.org/10.15446/anpol.v31n94.78239https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053407586&doi=10.7203%2fCIRIEC-E.93.10327&partnerID=40&md5=bf4e34a3b306679b0594288956a8839c08892229https://hdl.handle.net/20.500.12494/41726Hernández JC,Stevenson M,Latz E,Urcuqui S. HIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitro. AIDS Res Hum Retroviruses. 2012. 28. (10):p. 1313-1328. .Toll-like receptors (TLRs) play a critical role in innate immunity against pathogens. Their stimulation induces the activation of NF-?B, an important inducer of HIV-1 replication. In recent years, an increasing number of studies using several cells types from HIV-infected patients indicate that TLRs play a key role in regulating the expression of proinflammatory cytokines and viral pathogenesis. In the present study, the effect of HIV-1 stimulation of monocyte-derived macrophage (MDM) and peripheral blood mononuclear cell (PBMC) subpopulations from healthy donors on the expression and functions of TLR2 and TLR4 was examined. In addition, and to complete the in vitro study, the expression pattern of TLR2 and TLR4 in 49 HIV-1-infected patients, classified according to viral load and the use of HAART, was determined and compared with 25 healthy subjects. An increase of TLR expression and production of proinflammatory cytokines were observed in MDMs and PBMCs infected with HIV-1 in vitro and in response to TLR stimulation, compared to the mock. In addition, an association between TLR expression and up-regulation of CD80 in plasmacytoid dendritic cells (pDCs) was observed. The ex vivo analysis indicated increased expression of TLR2 and TLR4 in myeloid dendritic cells (mDCs), but only of TLR2 in monocytes obtained from HIV-1-infected patients, compared to healthy subjects. Remarkably, the expression was higher in cells from patients who do not use HAART. In monocytes, there was a positive correlation between both TLRs and viral load, but not CD4+ T cell numbers. Together, our in vitro and ex vivo results suggest that TLR expression and function can be up-regulated in response to HIV-1 infection and could affect the inflammatory response. We propose that modulation of TLRs represents a mechanism to promote HIV-1 replication or AIDS progression in HIV-1-infected patients. © 2012, Mary Ann Liebert, Inc.0000-0002-9200-5698juanc.hernandezl@campusucc.edu.co1328-1313Mary Ann Liebert Inc.abacaviramprenavirB7 antigenbeta actinCD123 antigenCD14 antigenCD4 antigencytokinedidanosineefavirenzfosamprenavirimmunoglobulin enhancer binding proteininterleukin 1betainterleukin 6lamivudinelopinavirmessenger RNAnelfinavirnevirapinesaquinavirstavudinetoll like receptor 2toll like receptor 4tumor necrosis factor alphazidovudineacquired immune deficiency syndromeadolescentadultarticleCD4+ T lymphocytecell subpopulationclinical articlecontrolled studycytokine productionex vivo studyfemalegene expression regulationhighly active antiretroviral therapyhumanhuman cellHuman immunodeficiency virus 1Human immunodeficiency virus 1 infectionHuman immunodeficiency virus infected patientimmune responseimmunomodulationin vitro studyin vivo studyinflammationinnate immunitymacrophagemalemonocytemyeloid dendritic cellnonhumanperipheral blood mononuclear cellplasmacytoid dendritic cellprioriHIV type 1 infection up-regulates TLR2 and TLR4 expression and function in vivo and in vitroArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionAIDS RES HUM RETROVinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41726oai:repository.ucc.edu.co:20.500.12494/417262024-08-20 16:18:17.692metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com |