Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.

Alternative splicing as a mean to control gene expression and diversify function is suspected to considerably influence drug response and clearance. We report the quantitative expression profiles of the human UGT genes including alternatively spliced variants not previously annotated established by...

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Autores:
Tourancheau, A
Rouleau, M
Guauque Olarte, Sandra Milena
Villeneuve, L
Gilbert, I
Droit, A
Guillemette, C
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
Idioma:
OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/41678
Acceso en línea:
https://doi.org/10.14349/rlp.2019.v51.n3.1
https://hdl.handle.net/20.500.12494/41678
Palabra clave:
glucuronosyltransferase
proteome
transcriptome
drug
glucuronosyltransferase
transcriptome
UGT1A1 enzyme
UGT2A1 protein
human
alternative RNA splicing
Article
carcinogenesis
controlled study
drug clearance
drug metabolism
drug response
female
gene
gene expression profiling
gene expression regulation
genetic variability
human
human tissue
interindividual variability
male
priority journal
RNA sequence
UGT gene
UGT1 gene
UGT2 gene
drug effect
genetics
metabolic clearance rate
metabolism
pharmacogenetic variant
physiology
procedures
tissue distribution
Gene Expression Profiling
Glucuronosyltransferase
Humans
Metabolic Clearance Rate
Pharmaceutical Preparations
Pharmacogenomic Variants
Tissue Distribution
Transcriptome
Rights
closedAccess
License
http://purl.org/coar/access_right/c_14cb
id COOPER2_b68d8a69d1bb59d512eb4ed852be83c4
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/41678
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Tourancheau, ARouleau, MGuauque Olarte, Sandra MilenaVilleneuve, LGilbert, IDroit, AGuillemette, C2021-12-16T22:15:42Z2021-12-16T22:15:42Z2018https://doi.org/10.14349/rlp.2019.v51.n3.11470269Xhttps://hdl.handle.net/20.500.12494/41678Tourancheau A,Rouleau M,Guauque S,Villeneuve L,Gilbert I,Droit A,Guillemette C. Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues. PHARMACOGENOMICS JOURNAL. 2018. 18. (2):p. 251-261. .Alternative splicing as a mean to control gene expression and diversify function is suspected to considerably influence drug response and clearance. We report the quantitative expression profiles of the human UGT genes including alternatively spliced variants not previously annotated established by deep RNA-sequencing in tissues of pharmacological importance. We reveal a comprehensive quantification of the alternative UGT transcriptome that differ across tissues and among individuals. Alternative transcripts that comprise novel in-frame sequences associated or not with truncations of the 5'- and/or 3'- termini, significantly contribute to the total expression levels of each UGT1 and UGT2 gene averaging 21% in normal tissues, with expression of UGT2 variants surpassing those of UGT1. Quantitative data expose preferential tissue expression patterns and remodeling in favor of alternative variants upon tumorigenesis. These complex alternative splicing programs have the strong potential to contribute to interindividual variability in drug metabolism in addition to diversify the UGT proteome.0000-0003-0336-9682sandra.guauque@campusucc.edu.co261-251Nature Publishing GroupglucuronosyltransferaseproteometranscriptomedrugglucuronosyltransferasetranscriptomeUGT1A1 enzymeUGT2A1 proteinhumanalternative RNA splicingArticlecarcinogenesiscontrolled studydrug clearancedrug metabolismdrug responsefemalegenegene expression profilinggene expression regulationgenetic variabilityhumanhuman tissueinterindividual variabilitymalepriority journalRNA sequenceUGT geneUGT1 geneUGT2 genedrug effectgeneticsmetabolic clearance ratemetabolismpharmacogenetic variantphysiologyprocedurestissue distributionGene Expression ProfilingGlucuronosyltransferaseHumansMetabolic Clearance RatePharmaceutical PreparationsPharmacogenomic VariantsTissue DistributionTranscriptomeQuantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPHARMACOGENOMICS JOURNALinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41678oai:repository.ucc.edu.co:20.500.12494/416782024-08-20 16:21:35.695metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
spellingShingle Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
glucuronosyltransferase
proteome
transcriptome
drug
glucuronosyltransferase
transcriptome
UGT1A1 enzyme
UGT2A1 protein
human
alternative RNA splicing
Article
carcinogenesis
controlled study
drug clearance
drug metabolism
drug response
female
gene
gene expression profiling
gene expression regulation
genetic variability
human
human tissue
interindividual variability
male
priority journal
RNA sequence
UGT gene
UGT1 gene
UGT2 gene
drug effect
genetics
metabolic clearance rate
metabolism
pharmacogenetic variant
physiology
procedures
tissue distribution
Gene Expression Profiling
Glucuronosyltransferase
Humans
Metabolic Clearance Rate
Pharmaceutical Preparations
Pharmacogenomic Variants
Tissue Distribution
Transcriptome
title_short Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title_full Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title_fullStr Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title_full_unstemmed Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
title_sort Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues.
dc.creator.fl_str_mv Tourancheau, A
Rouleau, M
Guauque Olarte, Sandra Milena
Villeneuve, L
Gilbert, I
Droit, A
Guillemette, C
dc.contributor.author.none.fl_str_mv Tourancheau, A
Rouleau, M
Guauque Olarte, Sandra Milena
Villeneuve, L
Gilbert, I
Droit, A
Guillemette, C
dc.subject.spa.fl_str_mv glucuronosyltransferase
proteome
transcriptome
drug
glucuronosyltransferase
transcriptome
UGT1A1 enzyme
UGT2A1 protein
human
alternative RNA splicing
Article
carcinogenesis
controlled study
drug clearance
drug metabolism
drug response
female
gene
gene expression profiling
gene expression regulation
genetic variability
human
human tissue
interindividual variability
male
priority journal
RNA sequence
UGT gene
UGT1 gene
UGT2 gene
drug effect
genetics
metabolic clearance rate
metabolism
pharmacogenetic variant
physiology
procedures
tissue distribution
Gene Expression Profiling
Glucuronosyltransferase
Humans
Metabolic Clearance Rate
Pharmaceutical Preparations
Pharmacogenomic Variants
Tissue Distribution
Transcriptome
topic glucuronosyltransferase
proteome
transcriptome
drug
glucuronosyltransferase
transcriptome
UGT1A1 enzyme
UGT2A1 protein
human
alternative RNA splicing
Article
carcinogenesis
controlled study
drug clearance
drug metabolism
drug response
female
gene
gene expression profiling
gene expression regulation
genetic variability
human
human tissue
interindividual variability
male
priority journal
RNA sequence
UGT gene
UGT1 gene
UGT2 gene
drug effect
genetics
metabolic clearance rate
metabolism
pharmacogenetic variant
physiology
procedures
tissue distribution
Gene Expression Profiling
Glucuronosyltransferase
Humans
Metabolic Clearance Rate
Pharmaceutical Preparations
Pharmacogenomic Variants
Tissue Distribution
Transcriptome
description Alternative splicing as a mean to control gene expression and diversify function is suspected to considerably influence drug response and clearance. We report the quantitative expression profiles of the human UGT genes including alternatively spliced variants not previously annotated established by deep RNA-sequencing in tissues of pharmacological importance. We reveal a comprehensive quantification of the alternative UGT transcriptome that differ across tissues and among individuals. Alternative transcripts that comprise novel in-frame sequences associated or not with truncations of the 5'- and/or 3'- termini, significantly contribute to the total expression levels of each UGT1 and UGT2 gene averaging 21% in normal tissues, with expression of UGT2 variants surpassing those of UGT1. Quantitative data expose preferential tissue expression patterns and remodeling in favor of alternative variants upon tumorigenesis. These complex alternative splicing programs have the strong potential to contribute to interindividual variability in drug metabolism in addition to diversify the UGT proteome.
publishDate 2018
dc.date.issued.none.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2021-12-16T22:15:42Z
dc.date.available.none.fl_str_mv 2021-12-16T22:15:42Z
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.14349/rlp.2019.v51.n3.1
dc.identifier.issn.spa.fl_str_mv 1470269X
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/41678
dc.identifier.bibliographicCitation.spa.fl_str_mv Tourancheau A,Rouleau M,Guauque S,Villeneuve L,Gilbert I,Droit A,Guillemette C. Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues. PHARMACOGENOMICS JOURNAL. 2018. 18. (2):p. 251-261. .
url https://doi.org/10.14349/rlp.2019.v51.n3.1
https://hdl.handle.net/20.500.12494/41678
identifier_str_mv 1470269X
Tourancheau A,Rouleau M,Guauque S,Villeneuve L,Gilbert I,Droit A,Guillemette C. Quantitative profiling of the UGT transcriptome in human drug-metabolizing tissues. PHARMACOGENOMICS JOURNAL. 2018. 18. (2):p. 251-261. .
dc.relation.ispartofjournal.spa.fl_str_mv PHARMACOGENOMICS JOURNAL
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv 261-251
dc.publisher.spa.fl_str_mv Nature Publishing Group
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
_version_ 1814247166165647360

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