Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1

Defensins, RNases and cytokines are present at mucosal barriers, main ports of HIV entry, and are potential mediators of the resistant phenotype exhibited by HIV-1-exposed seronegative individuals (HESN) during sexual exposure. We aimed to determine the role of soluble factors in natural resistance...

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Autores:: Zapata Builes, Wildeman
Aguilar-Jiménez W.
Feng Z.
Weinberg A.
Russo A.
Potenza N.
Estrada H.
Rugeles M.T.

Tipo de recurso:: Article of journal

Fecha de publicación:: 2016

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

Idioma:

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repository_id_str
spelling	Zapata Builes, WildemanAguilar-Jiménez W.Feng Z.Weinberg A.Russo A.Potenza N.Estrada H.Rugeles M.T.2021-12-16T22:15:34Z2021-12-16T22:15:34Z2016https://doi.org/10.1016/j.vetmic.2016.12.03912864579https://hdl.handle.net/20.500.12494/41505Zapata W,Aguilar W,Feng Z,Weinberg A,Russo A,Potenza N,Estrada H,Rugeles MT. Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1. MICROBES AND INFECTION. 2016. 18. (3):p. 211-219. .Defensins, RNases and cytokines are present at mucosal barriers, main ports of HIV entry, and are potential mediators of the resistant phenotype exhibited by HIV-1-exposed seronegative individuals (HESN) during sexual exposure. We aimed to determine the role of soluble factors in natural resistance to HIV-1 infection. Vaginal/endocervical/oral mucosal samples were taken from 60 HESN, 60 seropositive (SP) and 61 healthy controls (HC). Human neutrophil peptide 1 (hNP-1), human beta defensin (hBD) 2 and 3, RNases, MIP-1ß and RANTES mRNA transcripts were quantified by qPCR and in vitro single-round, recombinant-based viral infectivity assay was used to evaluate the anti-HIV-1 activity of hBDs and RNases. HESN expressed significantly higher levels of hNP-1, hBDs mRNA in oral mucosa compared to HC (P < 0.05). In genital mucosa, significantly higher mRNA levels of MIP-1ß, RANTES and RNases were found in HESN compared to HC (P < 0.05). HBDs and RNases inhibit HIV-1 replication, particularly R5 at entry, reverse transcription and nuclear import of the viral life cycle. hNP-1, hBDs, MIP-1ß, RANTES and RNases, collectively could contribute to HIV-1 resistance during sexual exposure. Moreover, the inhibition of HIV-1 infection in vitro by hBDs and RNases suggests that they may be exploited as potential antiretrovirals. © 2015 Institut Pasteur.0000-0002-7351-8738wildeman.zapatab@campusucc.edu.co219-211Elsevier Masson SASangiogeninbeta defensin 2beta defensin 3macrophage inflammatory protein 1betamaravirocmessenger RNAn [4 [[[6,7 dihydro 2 (4 methylphenyl) 5h benzocyclohepten 8 yl]carbonyl]amino]benzyl] n,n dimethyl 2h tetrahydropyran 4 aminium chlorideneutrophil peptide 1plerixaforproteinRANTESribonucleaseunclassified drugzidovudineimmunologic factoradultantiviral activityArticleCD4+ T lymphocytecontrolled studydose responsehumanHuman immunodeficiency virus 1Human immunodeficiency virus 1 infectionin vitro studyin vivo studyinnate immunitylife cyclemajor clinical studymouth mucosapolymerase chain reactionpriority journalprotein analysisprotein expressionprotein functionquantitative analysisreverse transcriptionsexual intercourseuterine cervix mucosavagina mucosaviral tropismvirus entryvirus infectivityvirus inhibitionvirus loadvirus replicationvirus resistancevirus strainadolescentdisease resistancefemaIdentification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1Artículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionMICROBES AND INFECTIONinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41505oai:repository.ucc.edu.co:20.500.12494/415052024-08-20 16:18:17.366metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv	Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title	Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
spellingShingle	Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1 angiogenin beta defensin 2 beta defensin 3 macrophage inflammatory protein 1beta maraviroc messenger RNA n [4 [[[6,7 dihydro 2 (4 methylphenyl) 5h benzocyclohepten 8 yl]carbonyl]amino]benzyl] n,n dimethyl 2h tetrahydropyran 4 aminium chloride neutrophil peptide 1 plerixafor protein RANTES ribonuclease unclassified drug zidovudine immunologic factor adult antiviral activity Article CD4+ T lymphocyte controlled study dose response human Human immunodeficiency virus 1 Human immunodeficiency virus 1 infection in vitro study in vivo study innate immunity life cycle major clinical study mouth mucosa polymerase chain reaction priority journal protein analysis protein expression protein function quantitative analysis reverse transcription sexual intercourse uterine cervix mucosa vagina mucosa viral tropism virus entry virus infectivity virus inhibition virus load virus replication virus resistance virus strain adolescent disease resistance fema
title_short	Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title_full	Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title_fullStr	Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title_full_unstemmed	Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
title_sort	Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1
dc.creator.fl_str_mv	Zapata Builes, Wildeman Aguilar-Jiménez W. Feng Z. Weinberg A. Russo A. Potenza N. Estrada H. Rugeles M.T.
dc.contributor.author.none.fl_str_mv	Zapata Builes, Wildeman Aguilar-Jiménez W. Feng Z. Weinberg A. Russo A. Potenza N. Estrada H. Rugeles M.T.
dc.subject.spa.fl_str_mv	angiogenin beta defensin 2 beta defensin 3 macrophage inflammatory protein 1beta maraviroc messenger RNA n [4 [[[6,7 dihydro 2 (4 methylphenyl) 5h benzocyclohepten 8 yl]carbonyl]amino]benzyl] n,n dimethyl 2h tetrahydropyran 4 aminium chloride neutrophil peptide 1 plerixafor protein RANTES ribonuclease unclassified drug zidovudine immunologic factor adult antiviral activity Article CD4+ T lymphocyte controlled study dose response human Human immunodeficiency virus 1 Human immunodeficiency virus 1 infection in vitro study in vivo study innate immunity life cycle major clinical study mouth mucosa polymerase chain reaction priority journal protein analysis protein expression protein function quantitative analysis reverse transcription sexual intercourse uterine cervix mucosa vagina mucosa viral tropism virus entry virus infectivity virus inhibition virus load virus replication virus resistance virus strain adolescent disease resistance fema
topic	angiogenin beta defensin 2 beta defensin 3 macrophage inflammatory protein 1beta maraviroc messenger RNA n [4 [[[6,7 dihydro 2 (4 methylphenyl) 5h benzocyclohepten 8 yl]carbonyl]amino]benzyl] n,n dimethyl 2h tetrahydropyran 4 aminium chloride neutrophil peptide 1 plerixafor protein RANTES ribonuclease unclassified drug zidovudine immunologic factor adult antiviral activity Article CD4+ T lymphocyte controlled study dose response human Human immunodeficiency virus 1 Human immunodeficiency virus 1 infection in vitro study in vivo study innate immunity life cycle major clinical study mouth mucosa polymerase chain reaction priority journal protein analysis protein expression protein function quantitative analysis reverse transcription sexual intercourse uterine cervix mucosa vagina mucosa viral tropism virus entry virus infectivity virus inhibition virus load virus replication virus resistance virus strain adolescent disease resistance fema
description	Defensins, RNases and cytokines are present at mucosal barriers, main ports of HIV entry, and are potential mediators of the resistant phenotype exhibited by HIV-1-exposed seronegative individuals (HESN) during sexual exposure. We aimed to determine the role of soluble factors in natural resistance to HIV-1 infection. Vaginal/endocervical/oral mucosal samples were taken from 60 HESN, 60 seropositive (SP) and 61 healthy controls (HC). Human neutrophil peptide 1 (hNP-1), human beta defensin (hBD) 2 and 3, RNases, MIP-1ß and RANTES mRNA transcripts were quantified by qPCR and in vitro single-round, recombinant-based viral infectivity assay was used to evaluate the anti-HIV-1 activity of hBDs and RNases. HESN expressed significantly higher levels of hNP-1, hBDs mRNA in oral mucosa compared to HC (P < 0.05). In genital mucosa, significantly higher mRNA levels of MIP-1ß, RANTES and RNases were found in HESN compared to HC (P < 0.05). HBDs and RNases inhibit HIV-1 replication, particularly R5 at entry, reverse transcription and nuclear import of the viral life cycle. hNP-1, hBDs, MIP-1ß, RANTES and RNases, collectively could contribute to HIV-1 resistance during sexual exposure. Moreover, the inhibition of HIV-1 infection in vitro by hBDs and RNases suggests that they may be exploited as potential antiretrovirals. © 2015 Institut Pasteur.
publishDate	2016
dc.date.issued.none.fl_str_mv	2016
dc.date.accessioned.none.fl_str_mv	2021-12-16T22:15:34Z
dc.date.available.none.fl_str_mv	2021-12-16T22:15:34Z
dc.type.none.fl_str_mv	Artículo
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv	http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv	info:eu-repo/semantics/publishedVersion
format	http://purl.org/coar/resource_type/c_6501
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://doi.org/10.1016/j.vetmic.2016.12.039
dc.identifier.issn.spa.fl_str_mv	12864579
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/41505
dc.identifier.bibliographicCitation.spa.fl_str_mv	Zapata W,Aguilar W,Feng Z,Weinberg A,Russo A,Potenza N,Estrada H,Rugeles MT. Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1. MICROBES AND INFECTION. 2016. 18. (3):p. 211-219. .
url	https://doi.org/10.1016/j.vetmic.2016.12.039 https://hdl.handle.net/20.500.12494/41505
identifier_str_mv	12864579 Zapata W,Aguilar W,Feng Z,Weinberg A,Russo A,Potenza N,Estrada H,Rugeles MT. Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1. MICROBES AND INFECTION. 2016. 18. (3):p. 211-219. .
dc.relation.ispartofjournal.spa.fl_str_mv	MICROBES AND INFECTION
dc.rights.accessrights.none.fl_str_mv	info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv	http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv	closedAccess
rights_invalid_str_mv	http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv	219-211
dc.publisher.spa.fl_str_mv	Elsevier Masson SAS
institution	Universidad Cooperativa de Colombia
repository.name.fl_str_mv	Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv	bdigital@metabiblioteca.com
_version_	1814246850875621376

Identification of innate immune antiretroviral factors during in vivo and in vitro exposure to HIV-1

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