The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death

Strongyloidiasis is widely distributed in the tropical and subtropical areas. Ivermectin is the drug of choice for the treatment. However, the concerns about relying treatment on a single drug make identification of new molecules a priority. Alkylphospholipid analogues, including edelfosine, are a g...

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Autores:: Legarda-Ceballos A.L.
Rojas Caraballo, Jose Vicente
López-Abán J.
Ruano A.L.
Yepes E.
Gajate C.
Mollinedo F.
Muro A.

Tipo de recurso:: Article of journal

Fecha de publicación:: 2016

Institución:: Universidad Cooperativa de Colombia

Repositorio:: Repositorio UCC

Idioma:

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network_acronym_str	COOPER2
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repository_id_str
spelling	Legarda-Ceballos A.L.Rojas Caraballo, Jose VicenteLópez-Abán J.Ruano A.L.Yepes E.Gajate C.Mollinedo F.Muro A.2021-12-16T22:15:30Z2021-12-16T22:15:30Z2016https://doi.org/10.1016/j.dental.2012.09.002https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062607238&doi=10.1371%2fjournal.pone.0213425&partnerID=40&md5=18df756332a9c86d62ca9bbc4087c4d10001706Xhttps://hdl.handle.net/20.500.12494/41416Legarda AL,Rojas J,López J,Ruano AL,Yepes E,Gajate C,Mollinedo F,Muro A. The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death. ACTA TROP. 2016. 162. p. 180-187. .Strongyloidiasis is widely distributed in the tropical and subtropical areas. Ivermectin is the drug of choice for the treatment. However, the concerns about relying treatment on a single drug make identification of new molecules a priority. Alkylphospholipid analogues, including edelfosine, are a group of synthetic compounds that have shown activity against some parasites. The objective was to assess the in vitro and in vivo activity of edelfosine, miltefosine, perifosine against Strongyloides venezuelensis. Moreover, apoptosis-like mechanism in larvae after treatment was studied. Edelfosine displayed the highest activity and the best selectivity index (LD50 = 49.6 ± 5.4 µM, SI = 1.1) compared to miltefosine or perifosine. Third stage larvae after culture with edelfosine were not able to develop an infection in mice. Treatment of mice with edelfosine showed reduction of 47% in parasitic females allocated in the gut. Moreover, DNA fragmentation was observed by TUNEL staining in larvae treated with edelfosine. These results suggest that edelfosine could be an effective drug against strongyloidiasis, probably through induction of apoptosis-like cell death. © 2016 Elsevier B.V.josev.rojas@campusucc.edu.co187-180Elsevieredelfosineivermectinmiltefosineperifosineantineoplastic agentedelfosineether phospholipidphosphorylcholineapoptosisDNAdrugfragmentationlarvanematodeparasitephospholipidanimal cellanimal experimentanimal modelantiprotozoal activityapoptosisArticlecell countDNA fragmentationdrug cytotoxicitydrug efficacydrug responsedrug structuredrug tolerabilityendothelium cellfibroblastIC50in vitro studyin vivo studylarvaLD50liver cellmousenonhumanStrongyloides venezuelensisstrongyloidiasistreatment durationanalogs and derivativesanimaldose responsedrug effectsfemaleparasitologyStrongyloidesstrongyloidiasisMusStrongyloides venezuelensisAnimalsAntineoplastic AgentsApoptosisDose-Response RelationshipDrugFemaleLarvaMicePhospholipid EthersPhosphorylcholineStrongyloidesStrongyloidiasisThe alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell deathArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionACTA TROPinfo:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbPublication20.500.12494/41416oai:repository.ucc.edu.co:20.500.12494/414162024-08-20 16:19:45.293metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv	The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death
title	The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death
spellingShingle	The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death edelfosine ivermectin miltefosine perifosine antineoplastic agent edelfosine ether phospholipid phosphorylcholine apoptosis DNA drug fragmentation larva nematode parasite phospholipid animal cell animal experiment animal model antiprotozoal activity apoptosis Article cell count DNA fragmentation drug cytotoxicity drug efficacy drug response drug structure drug tolerability endothelium cell fibroblast IC50 in vitro study in vivo study larva LD50 liver cell mouse nonhuman Strongyloides venezuelensis strongyloidiasis treatment duration analogs and derivatives animal dose response drug effects female parasitology Strongyloides strongyloidiasis Mus Strongyloides venezuelensis Animals Antineoplastic Agents Apoptosis Dose-Response Relationship Drug Female Larva Mice Phospholipid Ethers Phosphorylcholine Strongyloides Strongyloidiasis
title_short	The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death
title_full	The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death
title_fullStr	The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death
title_full_unstemmed	The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death
title_sort	The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death
dc.creator.fl_str_mv	Legarda-Ceballos A.L. Rojas Caraballo, Jose Vicente López-Abán J. Ruano A.L. Yepes E. Gajate C. Mollinedo F. Muro A.
dc.contributor.author.none.fl_str_mv	Legarda-Ceballos A.L. Rojas Caraballo, Jose Vicente López-Abán J. Ruano A.L. Yepes E. Gajate C. Mollinedo F. Muro A.
dc.subject.spa.fl_str_mv	edelfosine ivermectin miltefosine perifosine antineoplastic agent edelfosine ether phospholipid phosphorylcholine apoptosis DNA drug fragmentation larva nematode parasite phospholipid animal cell animal experiment animal model antiprotozoal activity apoptosis Article cell count DNA fragmentation drug cytotoxicity drug efficacy drug response drug structure drug tolerability endothelium cell fibroblast IC50 in vitro study in vivo study larva LD50 liver cell mouse nonhuman Strongyloides venezuelensis strongyloidiasis treatment duration analogs and derivatives animal dose response drug effects female parasitology Strongyloides strongyloidiasis Mus Strongyloides venezuelensis Animals Antineoplastic Agents Apoptosis Dose-Response Relationship Drug Female Larva Mice Phospholipid Ethers Phosphorylcholine Strongyloides Strongyloidiasis
topic	edelfosine ivermectin miltefosine perifosine antineoplastic agent edelfosine ether phospholipid phosphorylcholine apoptosis DNA drug fragmentation larva nematode parasite phospholipid animal cell animal experiment animal model antiprotozoal activity apoptosis Article cell count DNA fragmentation drug cytotoxicity drug efficacy drug response drug structure drug tolerability endothelium cell fibroblast IC50 in vitro study in vivo study larva LD50 liver cell mouse nonhuman Strongyloides venezuelensis strongyloidiasis treatment duration analogs and derivatives animal dose response drug effects female parasitology Strongyloides strongyloidiasis Mus Strongyloides venezuelensis Animals Antineoplastic Agents Apoptosis Dose-Response Relationship Drug Female Larva Mice Phospholipid Ethers Phosphorylcholine Strongyloides Strongyloidiasis
description	Strongyloidiasis is widely distributed in the tropical and subtropical areas. Ivermectin is the drug of choice for the treatment. However, the concerns about relying treatment on a single drug make identification of new molecules a priority. Alkylphospholipid analogues, including edelfosine, are a group of synthetic compounds that have shown activity against some parasites. The objective was to assess the in vitro and in vivo activity of edelfosine, miltefosine, perifosine against Strongyloides venezuelensis. Moreover, apoptosis-like mechanism in larvae after treatment was studied. Edelfosine displayed the highest activity and the best selectivity index (LD50 = 49.6 ± 5.4 µM, SI = 1.1) compared to miltefosine or perifosine. Third stage larvae after culture with edelfosine were not able to develop an infection in mice. Treatment of mice with edelfosine showed reduction of 47% in parasitic females allocated in the gut. Moreover, DNA fragmentation was observed by TUNEL staining in larvae treated with edelfosine. These results suggest that edelfosine could be an effective drug against strongyloidiasis, probably through induction of apoptosis-like cell death. © 2016 Elsevier B.V.
publishDate	2016
dc.date.issued.none.fl_str_mv	2016
dc.date.accessioned.none.fl_str_mv	2021-12-16T22:15:30Z
dc.date.available.none.fl_str_mv	2021-12-16T22:15:30Z
dc.type.none.fl_str_mv	Artículo
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv	http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv	info:eu-repo/semantics/publishedVersion
format	http://purl.org/coar/resource_type/c_6501
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://doi.org/10.1016/j.dental.2012.09.002 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062607238&doi=10.1371%2fjournal.pone.0213425&partnerID=40&md5=18df756332a9c86d62ca9bbc4087c4d1
dc.identifier.issn.spa.fl_str_mv	0001706X
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12494/41416
dc.identifier.bibliographicCitation.spa.fl_str_mv	Legarda AL,Rojas J,López J,Ruano AL,Yepes E,Gajate C,Mollinedo F,Muro A. The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death. ACTA TROP. 2016. 162. p. 180-187. .
url	https://doi.org/10.1016/j.dental.2012.09.002 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062607238&doi=10.1371%2fjournal.pone.0213425&partnerID=40&md5=18df756332a9c86d62ca9bbc4087c4d1 https://hdl.handle.net/20.500.12494/41416
identifier_str_mv	0001706X Legarda AL,Rojas J,López J,Ruano AL,Yepes E,Gajate C,Mollinedo F,Muro A. The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death. ACTA TROP. 2016. 162. p. 180-187. .
dc.relation.ispartofjournal.spa.fl_str_mv	ACTA TROP
dc.rights.accessrights.none.fl_str_mv	info:eu-repo/semantics/closedAccess
dc.rights.coar.none.fl_str_mv	http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv	closedAccess
rights_invalid_str_mv	http://purl.org/coar/access_right/c_14cb
dc.format.extent.spa.fl_str_mv	187-180
dc.publisher.spa.fl_str_mv	Elsevier
institution	Universidad Cooperativa de Colombia
repository.name.fl_str_mv	Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv	bdigital@metabiblioteca.com
_version_	1814246987367710720

The alkylphospholipid edelfosine shows activity against Strongyloides venezuelensis and induces apoptosis-like cell death

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