Biochemical and biological characterization of two serine proteinases from Colombian Crotalus durissus cumanensis snake venom

Two clotting serine proteinases, named Cdc SI and Cdc SII, were isolated and characterized for the first time from Colombian Crotalus durissus cumanensis snake venom. The enzymes were purified using two chromatographic steps: molecular exclusion on Sephacryl S-200 and RP-HPLC on C8 Column. The molec...

Full description

Autores:

Patiño A.C.
Pereañez, Jaime Andrés
Gutiérrez J.M.
Rucavado A.

Tipo de recurso:

Article of journal

Fecha de publicación:

2013

Institución:

Universidad Cooperativa de Colombia

Repositorio:

Repositorio UCC

Idioma:

OAI Identifier:

oai:repository.ucc.edu.co:20.500.12494/41608

Acceso en línea:

https://doi.org/10.1155/2016/1439090
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049342431&partnerID=40&md5=4f0c68f4526221cc091ec373cc5ecf0f
https://hdl.handle.net/20.500.12494/41608

Palabra clave:

Cdc SI enzyme
Cdc SII enzyme
Crotalus durissus cumanensis snake venom
fibrinogen
metalloproteinase
serine proteinase
snake venom
unclassified drug
amino terminal sequence
article
blood vessel permeability
chromatography
enzyme analysis
enzyme isolation
enzyme purification
hydrolysis
mass spectrometry
Michaelis Menten kinetics
molecular weight
mouse
nonhuman
priority journal
protein degradation
reversed phase high performance liquid chromatography
Amino Acid Sequence
Animals
Blood Coagulation
Capillary Permeability
Cattle
Chromatography
High Pressure Liquid
Coagulants
Crotalid Venoms
Crotalus
Edema
Hemorrhage
Humans
Mice
Molecular Sequence Data
Molecular Weight
Serine Proteases
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Bovinae
Crotalus durissus cumanensis

Rights

closedAccess

License

http://purl.org/coar/access_right/c_14cb