Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels

Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium i...

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Autores:
Estrada Gomez, Sebastian
Caldas Cardoso, Fernanda
Vargas Muñoz, Leidy Johana
Quintana Castillo, Juan Carlos
Arenas Gómez, Claudia Marcela
Pineda, Sandy Steffany
Saldarriaga Cordoba, Monica Maria
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad Cooperativa de Colombia
Repositorio:
Repositorio UCC
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OAI Identifier:
oai:repository.ucc.edu.co:20.500.12494/50841
Acceso en línea:
https://doi.org/doi.org/10.3390/toxins11090496
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071652937&doi=10.3390%2ftoxins11090496&partnerID=40&md5=3d9045d553ead0e2a35dd6a062ccefa7
https://hdl.handle.net/20.500.12494/50841
Palabra clave:
DISULFIDE-RICH PEPTIDE (DRP)
INHIBITORY CYSTEINE KNOT (ICK)
ION CHANNELS
PAMPHOBETEUS
PEPTIDES
THERAPHOSIDAE
TRANSCRIPTOME
VENOMICS
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openAccess
License
http://purl.org/coar/access_right/c_abf2
id COOPER2_0b43fe06742a3b5546ab657ad030a0fc
oai_identifier_str oai:repository.ucc.edu.co:20.500.12494/50841
network_acronym_str COOPER2
network_name_str Repositorio UCC
repository_id_str
spelling Estrada Gomez, SebastianCaldas Cardoso, FernandaVargas Muñoz, Leidy JohanaQuintana Castillo, Juan CarlosArenas Gómez, Claudia MarcelaPineda, Sandy SteffanySaldarriaga Cordoba, Monica Maria2023-05-24T16:30:09Z2023-05-24T16:30:09Z27/08/2019https://doi.org/doi.org/10.3390/toxins11090496https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071652937&doi=10.3390%2ftoxins11090496&partnerID=40&md5=3d9045d553ead0e2a35dd6a062ccefa720726651https://hdl.handle.net/20.500.12494/50841Estrada Gomez Sebastian,Caldas Cardoso Fernanda,Vargas Muñoz Leidy johana,Quintana Castillo Juan carlos,Arenas Gómez Claudia Marcela,Pineda Sandy Steffany,Saldarriaga Cordoba Monica Maria.Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels.Toxins. 2019. 11. (496): 496Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium influx assays, tandem mass spectrometry analysis (tMS/MS), and venom-gland transcriptome. rp-HPLC fractions of P. verdolaga venom showed activity on Ca V 2.2, Ca V 3.2, and Na V 1.7 ion channels. Active fractions contained several peptides with molecular masses ranging from 3399.4 to 3839.6 Da. The tMS/MS analysis of active fraction displaying the strongest activity to inhibit calcium channels showed sequence fragments similar to one of the translated transcripts detected in the venom-gland transcriptome. The putative peptide of this translated transcript corresponded to a toxin, here named ?-theraphositoxin-Pv3a, a potential ion channel modulator toxin that is, in addition, very similar to other theraphositoxins affecting calcium channels (i.e., ?-theraphotoxin-Asp1a). Additionally, using this holistic approach, we found that P. verdolaga venom is an important source of disulfide-rich proteins expressing at least eight superfamilies.0000-0002-7923-9158leidy.vargasmu@campusucc.edu.cojuan.quintanac@campusucc.edu.co496MDPI AGDISULFIDE-RICH PEPTIDE (DRP)INHIBITORY CYSTEINE KNOT (ICK)ION CHANNELSPAMPHOBETEUSPEPTIDESTHERAPHOSIDAETRANSCRIPTOMEVENOMICSVenomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium ChannelsArtículohttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionToxinsinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Publication20.500.12494/50841oai:repository.ucc.edu.co:20.500.12494/508412024-08-20 16:19:37.516metadata.onlyhttps://repository.ucc.edu.coRepositorio Institucional Universidad Cooperativa de Colombiabdigital@metabiblioteca.com
dc.title.spa.fl_str_mv Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
spellingShingle Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
DISULFIDE-RICH PEPTIDE (DRP)
INHIBITORY CYSTEINE KNOT (ICK)
ION CHANNELS
PAMPHOBETEUS
PEPTIDES
THERAPHOSIDAE
TRANSCRIPTOME
VENOMICS
title_short Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title_full Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title_fullStr Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title_full_unstemmed Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
title_sort Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels
dc.creator.fl_str_mv Estrada Gomez, Sebastian
Caldas Cardoso, Fernanda
Vargas Muñoz, Leidy Johana
Quintana Castillo, Juan Carlos
Arenas Gómez, Claudia Marcela
Pineda, Sandy Steffany
Saldarriaga Cordoba, Monica Maria
dc.contributor.author.none.fl_str_mv Estrada Gomez, Sebastian
Caldas Cardoso, Fernanda
Vargas Muñoz, Leidy Johana
Quintana Castillo, Juan Carlos
Arenas Gómez, Claudia Marcela
Pineda, Sandy Steffany
Saldarriaga Cordoba, Monica Maria
dc.subject.spa.fl_str_mv DISULFIDE-RICH PEPTIDE (DRP)
INHIBITORY CYSTEINE KNOT (ICK)
ION CHANNELS
PAMPHOBETEUS
PEPTIDES
THERAPHOSIDAE
TRANSCRIPTOME
VENOMICS
topic DISULFIDE-RICH PEPTIDE (DRP)
INHIBITORY CYSTEINE KNOT (ICK)
ION CHANNELS
PAMPHOBETEUS
PEPTIDES
THERAPHOSIDAE
TRANSCRIPTOME
VENOMICS
description Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium influx assays, tandem mass spectrometry analysis (tMS/MS), and venom-gland transcriptome. rp-HPLC fractions of P. verdolaga venom showed activity on Ca V 2.2, Ca V 3.2, and Na V 1.7 ion channels. Active fractions contained several peptides with molecular masses ranging from 3399.4 to 3839.6 Da. The tMS/MS analysis of active fraction displaying the strongest activity to inhibit calcium channels showed sequence fragments similar to one of the translated transcripts detected in the venom-gland transcriptome. The putative peptide of this translated transcript corresponded to a toxin, here named ?-theraphositoxin-Pv3a, a potential ion channel modulator toxin that is, in addition, very similar to other theraphositoxins affecting calcium channels (i.e., ?-theraphotoxin-Asp1a). Additionally, using this holistic approach, we found that P. verdolaga venom is an important source of disulfide-rich proteins expressing at least eight superfamilies.
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-05-24T16:30:09Z
dc.date.available.none.fl_str_mv 2023-05-24T16:30:09Z
dc.date.issued.none.fl_str_mv 27/08/2019
dc.type.none.fl_str_mv Artículo
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/ART
dc.type.version.none.fl_str_mv info:eu-repo/semantics/publishedVersion
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/doi.org/10.3390/toxins11090496
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071652937&doi=10.3390%2ftoxins11090496&partnerID=40&md5=3d9045d553ead0e2a35dd6a062ccefa7
dc.identifier.issn.spa.fl_str_mv 20726651
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12494/50841
dc.identifier.bibliographicCitation.spa.fl_str_mv Estrada Gomez Sebastian,Caldas Cardoso Fernanda,Vargas Muñoz Leidy johana,Quintana Castillo Juan carlos,Arenas Gómez Claudia Marcela,Pineda Sandy Steffany,Saldarriaga Cordoba Monica Maria.Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels.Toxins. 2019. 11. (496): 496
url https://doi.org/doi.org/10.3390/toxins11090496
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071652937&doi=10.3390%2ftoxins11090496&partnerID=40&md5=3d9045d553ead0e2a35dd6a062ccefa7
https://hdl.handle.net/20.500.12494/50841
identifier_str_mv 20726651
Estrada Gomez Sebastian,Caldas Cardoso Fernanda,Vargas Muñoz Leidy johana,Quintana Castillo Juan carlos,Arenas Gómez Claudia Marcela,Pineda Sandy Steffany,Saldarriaga Cordoba Monica Maria.Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels.Toxins. 2019. 11. (496): 496
dc.relation.ispartofjournal.spa.fl_str_mv Toxins
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
rights_invalid_str_mv http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 496
dc.publisher.spa.fl_str_mv MDPI AG
institution Universidad Cooperativa de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Cooperativa de Colombia
repository.mail.fl_str_mv bdigital@metabiblioteca.com
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