Venomic, Transcriptomic, and Bioactivity Analyses of Pamphobeteus verdolaga Venom Reveal Complex Disulfide-Rich Peptides That Modulate Calcium Channels

Pamphobeteus verdolaga is a recently described Theraphosidae spider from the Andean region of Colombia. Previous reports partially characterized its venom profile. In this study, we conducted a detailed analysis that includes reversed-phase high-performance liquid chromatography (rp-HPLC), calcium influx assays, tandem mass spectrometry analysis (tMS/MS), and venom-gland transcriptome. rp-HPLC fractions of P. verdolaga venom showed activity on Ca V 2.2, Ca V 3.2, and Na V 1.7 ion channels. Active fractions contained several peptides with molecular masses ranging from 3399.4 to 3839.6 Da. The tMS/MS analysis of active fraction displaying the strongest activity to inhibit calcium channels showed sequence fragments similar to one of the translated transcripts detected in the venom-gland transcriptome. The putative peptide of this translated transcript corresponded to a toxin, here named ?-theraphositoxin-Pv3a, a potential ion channel modulator toxin that is, in addition, very similar to other theraphositoxins affecting calcium channels (i.e., ?-theraphotoxin-Asp1a). Additionally, using this holistic approach, we found that P. verdolaga venom is an important source of disulfide-rich proteins expressing at least eight superfamilies.

• Autores: Estrada Gomez, Sebastian; Caldas Cardoso, Fernanda; Vargas Muñoz, Leidy Johana; Quintana Castillo, Juan Carlos; Arenas Gómez, Claudia Marcela; Pineda, Sandy Steffany; Saldarriaga Cordoba, Monica Maria
• Tipo de recurso: Article of journal
• Fecha de publicación: 2023
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/50841
• Acceso en línea: https://doi.org/doi.org/10.3390/toxins11090496; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85071652937&doi=10.3390%2ftoxins11090496&partnerID=40&md5=3d9045d553ead0e2a35dd6a062ccefa7; https://hdl.handle.net/20.500.12494/50841
• Palabra clave: DISULFIDE-RICH PEPTIDE (DRP); INHIBITORY CYSTEINE KNOT (ICK); ION CHANNELS; PAMPHOBETEUS; PEPTIDES; THERAPHOSIDAE; TRANSCRIPTOME; VENOMICS
• Rights: openAccess
• License: http://purl.org/coar/access_right/c_abf2