High-throughput sequencing reveals circulating miRNAs as potential biomarkers of kidney damage in patients with systemic lupus erythematosus

Renal involvement is one of the most severe manifestations of systemic lupus erythematosus (SLE). Renal biopsy is the gold standard when it comes to knowing whether a patient has lupus nephritis, and the degree of renal disease present. However, the biopsy has various complications, bleeding being the most common. Therefore, the development of alternative, non-invasive diagnostic tests for kidney disease in patients with SLE is a priority. Micro RNAs (miRNAs) are differentially expressed in various tissues, and changes in their expression have been associated with several pathological processes. The aim of this study was to identify changes in the abundance of miRNAs in plasma samples from patients with lupus nephritis that could potentially allow the diagnosis of renal damage in SLE patients. This is an observational case-control cross-sectional study, in which we characterized the differential abundance profiles of miRNAs among patients with different degrees of lupus compared with SLE patients without renal involvement and healthy control individuals. We found 89 miRNAs with changes in their abundance between lupus nephritis patients and healthy controls, and 17 miRNAs that showed significant variations between SLE patients with or without renal involvement. Validation for qPCR of a group of miRNAs on additional samples from lupus patients with or without nephritis, and from healthy individuals, showed that five miRNAs presented an average detection sensitivity of 97%, a specificity of 70.3%, a positive predictive value of 82.5%, a negative predictive value of 96% and a diagnosis efficiency of 87.9%. These results strongly suggest that miR-221-5p, miR-380-3p, miR-556-5p, miR-758-3p and miR-3074-3p are potential diagnostic biomarkers of lupus nephritis in patients with SLE. The observed differential pattern of miRNA abundance may have functional implications in the pathophysiology of SLE renal damage. © 2016 Navarro-Quiroz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

• Autores: Navarro-Quiroz E.; Pacheco-Lugo L.; Lorenzi H.; Díaz-Olmos Y.; Almendrales L.; Rico E.; Navarro Quiroz, Roberto Carlos; España-Puccini P.; Iglesias A.; Egea E.; Aroca G.
• Tipo de recurso: Article of journal
• Fecha de publicación: 2016
• Institución: Universidad Cooperativa de Colombia
• Repositorio: Repositorio UCC
• OAI Identifier: oai:repository.ucc.edu.co:20.500.12494/41905
• Acceso en línea: https://doi.org/10.17533/udea.rfnsp.v35n1a10; https://hdl.handle.net/20.500.12494/41905
• Palabra clave: microRNA; microRNA 221 5p; microRNA 3074 3p; microRNA 380 3p; microRNA 556 5p; microRNA 758 3p; unclassified drug; biological marker; microRNA; MIRN221 microRNA; human; MIRN3074 microRNA; human; MIRN380 microRNA; human; MIRN556 microRNA; human; MIRN758 microRNA; human; area under the curve; Article; blood sampling; case control study; cross-sectional study; diagnostic accuracy; diagnostic test accuracy study; disease association; disease marker; gene expression; high throughput screening; human; human cell; kidney injury; observational study; polymerase chain reaction; predictive value; receiver operating characteristic; sensitivity and specificity; systemic lupus erythematosus; validity; adult; blood; female; gene expression profiling; genetics; high throughput sequencing; lupus erythematosus nephritis; male; middle aged; principal component analysis; procedures; reverse transcription polymerase chain reaction; systemic lupus erythematosus; young adult; Adult; Biomarkers; Female; Gene
• Rights: closedAccess
• License: http://purl.org/coar/access_right/c_14cb