Efficient method for molecular characterization of the 5' and 3' ends of the dengue virus genome
Dengue is a mosquito-borne disease that is of major importance in public health. Although it has been extensively studied at the molecular level, sequencing of the 5' and 3' ends of the untranslated regions (UTR) commonly requires specific approaches for completion and corroboration. The p...
- Autores:
-
Rosales-Munar A.
Alvarez-Diaz D.A.
Laiton-Donato K.
Peláez-Carvajal D.
Usme Ciro, José Aldemar
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2020
- Institución:
- Universidad Cooperativa de Colombia
- Repositorio:
- Repositorio UCC
- Idioma:
- OAI Identifier:
- oai:repository.ucc.edu.co:20.500.12494/42996
- Acceso en línea:
- https://doi.org/10.7705/biomedica.v37i3.3170
https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954860766&doi=10.1007%2fs00784-009-0306-0&partnerID=40&md5=1a504adf4234e13c481b675095ad8429
https://hdl.handle.net/20.500.12494/42996
- Palabra clave:
- 3' untranslated region
5' untranslated region
Article
Dengue virus
DNA hybridization
DNA sequencing
DNA synthesis
Escherichia coli
gene amplification
gene sequence
genetic variability
high throughput sequencing
human
human cell
molecular biology
molecular cloning
nonhuman
polyadenylation
polymerase chain reaction
reverse transcription
reverse transcription polymerase chain reaction
RNA extraction
Sanger sequencing
sequence alignment
serotyping
virus detection
virus genome
virus isolation
virus replication
virus titration
- Rights
- closedAccess
- License
- http://purl.org/coar/access_right/c_14cb