Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando

El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en hum...

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Autores:: Vejarano-Orjuela, Álvaro
Hernández-Vásquez, Aureliano
Rondón-Barragán, Iang S.
Suárez-Mesa, Rafael A.

Tipo de recurso:: Article of journal

Fecha de publicación:: 2020

Institución:: Universidad de los Llanos

Repositorio:: Repositorio Digital Universidad de los LLanos

Idioma:: spa

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dc.title.spa.fl_str_mv	Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
dc.title.translated.eng.fl_str_mv	Luteinizing hormone choriogonadotropin receptor (LHGCR) gene polymorphism in ovarian follicles from cycling Romosinuano and Brahman cows
title	Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
spellingShingle	Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando bovine creole reproduction LHR–LHCGR fertility indicator bovinos criollo reproducción LHR–LHCGR reprodução de bovinos crioulos LHR–LHCGR
title_short	Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
title_full	Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
title_fullStr	Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
title_full_unstemmed	Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
title_sort	Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
dc.creator.fl_str_mv	Vejarano-Orjuela, Álvaro Hernández-Vásquez, Aureliano Rondón-Barragán, Iang S. Suárez-Mesa, Rafael A.
dc.contributor.author.spa.fl_str_mv	Vejarano-Orjuela, Álvaro Hernández-Vásquez, Aureliano Rondón-Barragán, Iang S. Suárez-Mesa, Rafael A.
dc.subject.eng.fl_str_mv	bovine creole reproduction LHR–LHCGR fertility indicator
topic	bovine creole reproduction LHR–LHCGR fertility indicator bovinos criollo reproducción LHR–LHCGR reprodução de bovinos crioulos LHR–LHCGR
dc.subject.spa.fl_str_mv	bovinos criollo reproducción LHR–LHCGR reprodução de bovinos crioulos LHR–LHCGR
description	El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ovario que contenía un folículo preovulatorio, se retiró la pared folicular, se extrajo el RNA total y se sintetizó, amplificó, secuenció y comparó el ADNc contra secuencias ya reportadas, presentando una alta similitud. Se concluye que en estas razas la ausencia de variantes en la expresión del gen, estaría asociada a buen desempeño reproductivo.
publishDate	2020
dc.date.accessioned.none.fl_str_mv	2020-05-11T00:00:00Z 2024-07-25T18:15:00Z
dc.date.available.none.fl_str_mv	2020-05-11T00:00:00Z 2024-07-25T18:15:00Z
dc.date.issued.none.fl_str_mv	2020-05-11
dc.type.spa.fl_str_mv	Artículo de revista
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dc.identifier.doi.none.fl_str_mv	10.22579/20112629.590
dc.identifier.eissn.none.fl_str_mv	2011-2629
dc.identifier.url.none.fl_str_mv	https://doi.org/10.22579/20112629.590
identifier_str_mv	0121-3709 10.22579/20112629.590 2011-2629
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dc.relation.references.spa.fl_str_mv	Cadagan D, Towlson C. Mechanisms of Luteinising Hormone Regulation in Female Steroidogenesis. Am J Med, 2017;5:65-68. Casarini L, Pignatti E, Simoni M. Effects of polymorphisms in gonadotropin and gonadotropin receptor genes on reproductive function. Rev Endocr Metab Dis, 2011;12:303. Clelland E, Peng C. Endocrine/paracrine control of zebrafish ovarian development. Mol Cell Endocrinol, 2009;312:42-52. Delemi A. Identificaion of the expression level to LH-r Gene in dominant and cystic ovarian follicles cells of the cow. Al-Delemi DHJ, Al-Gewary AKA, Ali Jeddoa Z. Identification of the expression level to LH-r gene in dominant and cystic ovarian follicles cells of the cows. J Dairy Vet Anim Res, 2014;1(3):80-86. DOI: 10.15406/jdvar.2014.01.00017 Dickinson RE, Stewart AJ, Myers M, Millar RP Duncan WC. Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells: implications for luteolysis. Endocrinology, 2009;150:2873-2881. Gay V, Midgley Jr A, Niswender G. (1970). Patterns of gonadotrophin secretion associated with ovulation. Federation proceedings, p. 1880. Ginther O. Selection of the dominant follicle in cattle and horses. Anim Reprod Sci, 2000;60:61-79. Hastings N, Donn S, Derecka K, Flint A, Woolliams J. Polymorphisms within the coding region of the bovine luteinizing hormone receptor gene and their association with fertility traits. Anim Genet, 2006;37:583-585. Holdridge LR. 1967. Life zone ecology. Life zone ecology. Johnson A, Bridgham J, Wagner B. Characterization of a chicken luteinizing hormone receptor (cLH-R) complementary deoxyribonucleic acid, and expression of cLH-R messenger ribonucleic acid in the ovary. Biol Reprod, 1996;55: 304-309. Kawate N. Studies on the regulation of expression of luteinizing hormone receptor in the ovary and the mechanism of follicular cyst formation in ruminants. J Reprod Develop, 2004;50:1-8. Larsson B. Determination of Ovulation by Ultrasound Examination and its Relation to the LH‐Peak in Heifers. J Vet Med A, 1987;34:749-754. Liu KC, Lin SW, Ge W. Differential regulation of gonadotropin receptors (fshr and lhcgr) by estradiol in the zebrafish ovary involves nuclear estrogen receptors that are likely located on the plasma membrane. Endocrinology, 2011;152:4418-4430. Ma T, Xiong Q, Yuan B, Jiang H, Gao Y, Xu J, Liu S, et al. Luteinizing hormone receptor splicing variants in bovine Leydig cells. Genet Mol Res, 2012;11:1721-1730. Mamluk R, Wolfenson D, Meidan R. LH receptor mRNA and cytochrome P450 side-chain cleavage expression in bovine theca and granulosa cells luteinized by LH or forskolin. Domest Anim Endocrinol, 1998;15:103-114. McArdle C, Roberson M. Gonadotropes and gonadotropin-releasing hormone signaling. Knobil and Neill’s Physiology of Reproduction, 2015;4:335-397. Nejad SZ, Tehrani FR, Zadeh-Vakili A. The Role of Kisspeptin in Female Reproduction. Int J Endocrinol Metab, 2017;15(3):e44337. doi: 10.5812/ijem.44337. Nogueira M, Buratini J, Price C, Castilho A, Pinto M, Barros C. Expression of LH receptor mRNA splice variants in bovine granulosa cells: changes with follicle size and regulation by FSH in vitro. Mol Reprod Dev, 2007;74: 680-686. Peiró JR, Nogueira GM, Nogueira GP, Perri SH, Cardoso D. Ovariectomy by left flank approach in prepubertal Nelore (Bos indicus) heifers. Can J Vet Res, 2009;73:237-240. Pierson R, Ginther O. Ultrasonic imaging of the ovaries and uterus in cattle. Theriogenology, 1988;29:21-37. Rawan A, Yoshioka S, Abe H, Acosta T. Insulin‐Like Growth Factor‐1 Regulates the Expression of Luteinizing Hormone Receptor and Steroid Production in Bovine Granulosa Cells. Reprod Domest Anim, 2015;50:283-291. Richards J, Hedin L. Molecular aspects of hormone action in ovarian follicular development, ovulation, and luteinization. Annu Rev Physiol, 1988;50: 441-463. Robert C, Gagne D, Lussier J, Bousquet D, Barnes F, Sirard M. Presence of LH receptor mRNA in granulosa cells as a potential marker of oocyte developmental competence and characterization of the bovine splicing isoforms. Reproduction, 2003;125:437-446. Senger (2012). Pathways to pregnancy and parturition. 3th ed. Redmond: Current Conceptions. Inc., p.102.
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dc.relation.ispartofjournal.spa.fl_str_mv	Orinoquia
dc.rights.spa.fl_str_mv	Orinoquia - 2020
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dc.publisher.spa.fl_str_mv	Universidad de los Llanos
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spelling	Vejarano-Orjuela, ÁlvaroHernández-Vásquez, AurelianoRondón-Barragán, Iang S.Suárez-Mesa, Rafael A.2020-05-11T00:00:00Z2024-07-25T18:15:00Z2020-05-11T00:00:00Z2024-07-25T18:15:00Z2020-05-110121-3709https://repositorio.unillanos.edu.co/handle/001/397410.22579/20112629.5902011-2629https://doi.org/10.22579/20112629.590El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ovario que contenía un folículo preovulatorio, se retiró la pared folicular, se extrajo el RNA total y se sintetizó, amplificó, secuenció y comparó el ADNc contra secuencias ya reportadas, presentando una alta similitud. Se concluye que en estas razas la ausencia de variantes en la expresión del gen, estaría asociada a buen desempeño reproductivo.The luteinizing hormone (LH) choriogonadotropin receptor (LHCGR) gene is highly conserved and its mRNA causes variants by alternative splicing. Variants have been reported in cattle that translate into protein; however, this does not cross the cell membrane. Exon 10 deletion in humans does not trigger a response to the action of LH; it is therefore reasonable to assume that differences regarding response to LH are related to LHR expression. This work was aimed at comparing the Colombian creole Romosinuano breed to the Brahman breed regarding possible HCGR gene mRNA polymorphisms as potential fertility indicators. Five cycling cows were taken from each breed and the ovaries containing pre-ovulatory follicles were excised, the follicular wall was removed and total RNA extracted. The cDNA was synthesised, amplified, sequenced and compared to already reported sequences, great similarity being observed. It was concluded that the lack of variants regarding gene expression in these breeds would be associated with good reproductive performance.application/pdfspaUniversidad de los LlanosOrinoquia - 2020https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/590bovine creole reproductionLHR–LHCGRfertility indicatorbovinos criollo reproducciónLHR–LHCGRreprodução de bovinos crioulosLHR–LHCGRPolimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclandoLuteinizing hormone choriogonadotropin receptor (LHGCR) gene polymorphism in ovarian follicles from cycling Romosinuano and Brahman cowsArtículo de revistainfo:eu-repo/semantics/articleJournal articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Texthttp://purl.org/coar/version/c_970fb48d4fbd8a85Cadagan D, Towlson C. Mechanisms of Luteinising Hormone Regulation in Female Steroidogenesis. Am J Med, 2017;5:65-68.Casarini L, Pignatti E, Simoni M. Effects of polymorphisms in gonadotropin and gonadotropin receptor genes on reproductive function. Rev Endocr Metab Dis, 2011;12:303.Clelland E, Peng C. Endocrine/paracrine control of zebrafish ovarian development. Mol Cell Endocrinol, 2009;312:42-52.Delemi A. Identificaion of the expression level to LH-r Gene in dominant and cystic ovarian follicles cells of the cow.Al-Delemi DHJ, Al-Gewary AKA, Ali Jeddoa Z. Identification of the expression level to LH-r gene in dominant and cystic ovarian follicles cells of the cows. J Dairy Vet Anim Res, 2014;1(3):80-86. DOI: 10.15406/jdvar.2014.01.00017Dickinson RE, Stewart AJ, Myers M, Millar RP Duncan WC. Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells: implications for luteolysis. Endocrinology, 2009;150:2873-2881.Gay V, Midgley Jr A, Niswender G. (1970). Patterns of gonadotrophin secretion associated with ovulation. Federation proceedings, p. 1880.Ginther O. Selection of the dominant follicle in cattle and horses. Anim Reprod Sci, 2000;60:61-79.Hastings N, Donn S, Derecka K, Flint A, Woolliams J. Polymorphisms within the coding region of the bovine luteinizing hormone receptor gene and their association with fertility traits. Anim Genet, 2006;37:583-585.Holdridge LR. 1967. Life zone ecology. Life zone ecology.Johnson A, Bridgham J, Wagner B. Characterization of a chicken luteinizing hormone receptor (cLH-R) complementary deoxyribonucleic acid, and expression of cLH-R messenger ribonucleic acid in the ovary. Biol Reprod, 1996;55: 304-309.Kawate N. Studies on the regulation of expression of luteinizing hormone receptor in the ovary and the mechanism of follicular cyst formation in ruminants. J Reprod Develop, 2004;50:1-8.Larsson B. Determination of Ovulation by Ultrasound Examination and its Relation to the LH‐Peak in Heifers. J Vet Med A, 1987;34:749-754.Liu KC, Lin SW, Ge W. Differential regulation of gonadotropin receptors (fshr and lhcgr) by estradiol in the zebrafish ovary involves nuclear estrogen receptors that are likely located on the plasma membrane. Endocrinology, 2011;152:4418-4430.Ma T, Xiong Q, Yuan B, Jiang H, Gao Y, Xu J, Liu S, et al. Luteinizing hormone receptor splicing variants in bovine Leydig cells. Genet Mol Res, 2012;11:1721-1730.Mamluk R, Wolfenson D, Meidan R. LH receptor mRNA and cytochrome P450 side-chain cleavage expression in bovine theca and granulosa cells luteinized by LH or forskolin. Domest Anim Endocrinol, 1998;15:103-114.McArdle C, Roberson M. Gonadotropes and gonadotropin-releasing hormone signaling. Knobil and Neill’s Physiology of Reproduction, 2015;4:335-397.Nejad SZ, Tehrani FR, Zadeh-Vakili A. The Role of Kisspeptin in Female Reproduction. Int J Endocrinol Metab, 2017;15(3):e44337. doi: 10.5812/ijem.44337.Nogueira M, Buratini J, Price C, Castilho A, Pinto M, Barros C. Expression of LH receptor mRNA splice variants in bovine granulosa cells: changes with follicle size and regulation by FSH in vitro. Mol Reprod Dev, 2007;74: 680-686.Peiró JR, Nogueira GM, Nogueira GP, Perri SH, Cardoso D. Ovariectomy by left flank approach in prepubertal Nelore (Bos indicus) heifers. Can J Vet Res, 2009;73:237-240.Pierson R, Ginther O. Ultrasonic imaging of the ovaries and uterus in cattle. Theriogenology, 1988;29:21-37.Rawan A, Yoshioka S, Abe H, Acosta T. Insulin‐Like Growth Factor‐1 Regulates the Expression of Luteinizing Hormone Receptor and Steroid Production in Bovine Granulosa Cells. Reprod Domest Anim, 2015;50:283-291.Richards J, Hedin L. Molecular aspects of hormone action in ovarian follicular development, ovulation, and luteinization. Annu Rev Physiol, 1988;50: 441-463.Robert C, Gagne D, Lussier J, Bousquet D, Barnes F, Sirard M. Presence of LH receptor mRNA in granulosa cells as a potential marker of oocyte developmental competence and characterization of the bovine splicing isoforms. Reproduction, 2003;125:437-446.Senger (2012). Pathways to pregnancy and parturition. 3th ed. Redmond: Current Conceptions. Inc., p.102.https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/590/pdf5114224OrinoquiaPublicationOREORE.xmltext/xml2823https://repositorio.unillanos.edu.co/bitstreams/7377028c-4c76-4b49-ad0f-827648389bd1/download2ca014e42c52f765ff77f5aa46ebef10MD51001/3974oai:repositorio.unillanos.edu.co:001/39742024-07-25 13:15:00.432https://creativecommons.org/licenses/by-nc-sa/4.0/Orinoquia - 2020metadata.onlyhttps://repositorio.unillanos.edu.coRepositorio Universidad de Los Llanosrepositorio@unillanos.edu.co

Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando

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