Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman
Incluye tablas y figuras.
- Autores:
-
Sierra Martinez, Zully Esmeralda
Baquero Morales, Viviana Alejandra
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Universidad de los Llanos
- Repositorio:
- Repositorio Digital Universidad de los LLanos
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unillanos.edu.co:001/3107
- Acceso en línea:
- https://repositorio.unillanos.edu.co/handle/001/3107
https://repositorio.unillanos.edu.co/
- Palabra clave:
- Biotecnología
bovinos
cebú
marcador endocrino
reproducción
Biotechnology
cattle
zebu
endocrine marker
reproduction
- Rights
- openAccess
- License
- Derechos Reservados - Universidad de los Llanos, año 2022
| id |
Unillanos2_db7fd6e71b6070d00809e051e5a2c60a |
|---|---|
| oai_identifier_str |
oai:repositorio.unillanos.edu.co:001/3107 |
| network_acronym_str |
Unillanos2 |
| network_name_str |
Repositorio Digital Universidad de los LLanos |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman |
| title |
Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman |
| spellingShingle |
Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman Biotecnología bovinos cebú marcador endocrino reproducción Biotechnology cattle zebu endocrine marker reproduction |
| title_short |
Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman |
| title_full |
Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman |
| title_fullStr |
Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman |
| title_full_unstemmed |
Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman |
| title_sort |
Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman |
| dc.creator.fl_str_mv |
Sierra Martinez, Zully Esmeralda Baquero Morales, Viviana Alejandra |
| dc.contributor.advisor.none.fl_str_mv |
Góngora Orjuela, Agustín |
| dc.contributor.author.none.fl_str_mv |
Sierra Martinez, Zully Esmeralda Baquero Morales, Viviana Alejandra |
| dc.subject.proposal.spa.fl_str_mv |
Biotecnología bovinos cebú marcador endocrino reproducción |
| topic |
Biotecnología bovinos cebú marcador endocrino reproducción Biotechnology cattle zebu endocrine marker reproduction |
| dc.subject.proposal.eng.fl_str_mv |
Biotechnology cattle zebu endocrine marker reproduction |
| description |
Incluye tablas y figuras. |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2023-10-23T21:53:39Z |
| dc.date.available.none.fl_str_mv |
2023-10-23T21:53:39Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Pregrado |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/bachelorThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_7a1f |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/TP |
| dc.type.coarversion.spa.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| format |
http://purl.org/coar/resource_type/c_7a1f |
| status_str |
publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Sierra Martínez, Z. E. y Baquero Morales, V. A. (2022). Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman [Trabajo de grado, Universidad de los Llanos]. Repositorio digital Universidad de los Llanos. |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unillanos.edu.co/handle/001/3107 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad de los Llanos |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio digital Universidad de los Llanos |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unillanos.edu.co/ |
| identifier_str_mv |
Sierra Martínez, Z. E. y Baquero Morales, V. A. (2022). Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman [Trabajo de grado, Universidad de los Llanos]. Repositorio digital Universidad de los Llanos. Universidad de los Llanos Repositorio digital Universidad de los Llanos |
| url |
https://repositorio.unillanos.edu.co/handle/001/3107 https://repositorio.unillanos.edu.co/ |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
Abbas, A. K.; Lichtman, A. H.; Pillai, S. (2012). Técnicas de laboratorio usadas con frecuencia en inmunología. https://scholar.google.com/scholar?hl=es&as_sdt=0%2C5&q=Abbas%2C+A.+K.%3B+Lichtman%2C+A.+H.%3B+Pillai%2C+S.+%282012%29.+Técnicas+d e+laboratorio+usadas+con+frecuencia+&btnG= Al -Chalabi, S. S., Al-Wattar, Y. T., & Algalili, I. M. (2012). Anti-Mullerian Hormone Is a Significant Marker for Male Infertility. In Tikrit Journal of Pharmaceutical Sciences (Vol. 8, Issue 1). https://www.iasj.net/iasj/download/6c40eab0268cfb9d Baruselli, P., Batista, E., & Ferreira, R. (2016). Niveles plasmáticos de hormona anti-mülleriana permite la selección de donadoras con alto potencial de producción de embriones. SPERMOVA, 1(6), 1–13. https://doi.org/10.18548/aspe/0003.01 Batista, E. O. S., Macedo, G. G., Sala, R. V., Ortolan, M. D. D. V., Sá Filho, M. F., Del Valle, T. A., Jesus, E. F., Lopes, R. N. V. R., Rennó, F. P., & Baruselli, P. S. (2014). Plasma antimullerian hormone as a predictor of ovarian antral follicular population in bos indicus (nelore) and bos taurus (holstein) heifers. Reproduction in Domestic Animals, 49(3), 448–452. https://doi.org/10.1111/rda.12304 Batista, Vieira, L. M., Sá Filho, M. F., Dias, E. A. R., Bayeux, B. M., Accorsi, M. F., Monteiro, F. M., Souza, A. H., Baruselli, P. S., & D’Occhio, M. J. (2016). Ovarian follicular growth suppression by long-term treatment with a GnRH agonist and impact on small follicle number, oocyte yield, and in vitro embryo production in Zebu beef cows. Theriogenology, 85(9), 1680–1687. https://doi.org/10.1016/j.theriogenology.2016.01.023 Batista, EB; Guerreiro, BG; Freitas, JC; Silva, LM; Vieira, RM; Ferreira, RG; Resende, AC; Basso, RN; Lopes, FP; Rennó, AH; Souza; Baruselli, PS. 2015. Plasma anti-Mullerian hormone as predictive endocrine marker for selection of Bos taurus (Holstein) and Bos indicus (Nelore) calves to in vitro embryo production. Submitted for publication. Bezard, J., Vigier, B., Tran, D., Mauleon, P., & Josso, N. (1987). Immunocytochemical study of anti-Mullerian hormone in sheep ovarian follicles during fetal and post-natal development. Reproduction, 80(2), 509–516. https://doi.org/10.1530/jrf.0.0800509 Block, E. (1952). Quantitative morphological investigations of the follicular system in women. Cells Tissues Organs, 14(1–2), 108–123. https://doi.org/10.1159/000140595 Borges, Á. M., Torres, C. A. A., Ruas, J. R. M., Rocha, V. R., & Carvalho, G. R. (2001). Ovarian follicular dynamics in crossbred Holstein-Zebu heifers. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 53(5), 595–604. https://doi.org/10.1590/s0102-09352001000500015 Bustillo, J., & Melo, J. (2020). Parámetros reproductivos y eficiencia reproductiva en ganado bovino reproductive parameters and reproductive efficiency in cattle. Casas, E., & Tewolde, A. (2001). Evaluación de características relacionadas con la eficiencia reproductiva de genotipos criollos de carne en el trópico húmedo. Climent Peris, S. (2013). Embriología y anatomía veterinaria. In Embriología y anatomía veterinaria. http://www.sidalc.net/cgibin/wxis.exe/?IsisScript=librosnl.xis&method=post&formato=2&cantidad=1&expresion=mfn=002490 Crowe, M. A., Goulding, D., Baguisi, A., Boland, M. P., & Roche, J. F. (1993). Induced ovulation of the first postpartum dominant follicle in beef suckler cows using a GnRH analogue. Journal of Reproduction and Fertility, 99(2), 551–555. https://doi.org/10.1530/jrf.0.0990551 Church, D. 1993. The Ruminant Animal: Digestive Physiology and Nutrition Corvallis, Zaragoza (España). pp. 525-537. Diskin, M. G., Austin, E. J., & Roche, J. F. (2002). Exogenous hormonal manipulation of ovarian activity in cattle. In Domestic Animal Endocrinology (Vol. 23). https://www.sciencedirect.com/science/article/pii/S0739724002001583 Durlinger, A., Visser, J., & Themmen, A. (2002). Regulation of ovarian function: the role of anti-Mullerian hormone. In Reproduction. Erickson, B. H. (1966). DEVELOPMENT AND RADIO-RESPONSE OF THE PRENATAL BOVINE OVARY. In Reproducción. https://rep.bioscientifica.com/view/journals/rep/11/1/jrf_11_1_011.xml Filipiak Y, Viqueira M, B. A. (2016). Desarrollo y dinámica de los folículos ováricos desde la etapa fetal hasta la prepuberal en bovinos. Desarrollo y Dinámica de Los Folículos Ováricos Desde La Etapa Fetal Hasta La Prepuberal En Bovinos, 14–22. FJ, G.-G., AS, S.-R., & J., A.-B. (2004). El factor de crecimiento transformante beta como blanco terapéutico. In Salud Publica Mex (Vol. 46). http://www.insp.mx/salud/index.html Fortune, J. (2003). The early stages of follicular development: activation of primordial follicles and growth of preantral follicles. Animal Reproduction, 78((3-4)), 135–163. https://www.sciencedirect.com/science/article/pii/S0378432003000885 Gan, S. D., & Patel, K. R. (2013). Enzyme Immunoassay and Enzyme-Linked Immunosorbent Assay. Article in Journal of Investigative Dermatology, 12. https://doi.org/10.1038/jid.2013.287 García, G. A., Maldonado-Estrada, J. G., & López, J. G. (2003). Caracterización productiva y reproductiva de las explotaciones ganaderas del bajo cauca y el litoral atlántico antioqueños. II. Comportamiento de cuatro grupos. Revista Colombiana de Ciencias Pecuarias, 16(2), 117–125. https://www.redalyc.org/pdf/2950/295026126002.pdf Gonzalez, K. (2017). La Raza de Ganado Brahman. Guerreiro, B., Batista, E., … L. V.-D. animal, & 2014, U. (2014). Plasma anti-mullerian hormone: an endocrine marker for in vitro embryo production from Bos taurus and Bos indicus donors. Elsevier. https://www.sciencedirect.com/science/article/pii/S0739724014000678 Hasler J.F. 2013. Forty years of embryo transfer in cattle: A review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces. Theriogenology. Vol;81(1,1):152–169. ICA. (2020). Censo pecuario nacional del ICA 2020. https://www.ica.gov.co/areas/pecuaria/servicios/epidemiologia-veterinaria/censos2016/censo-2018 Ireland, J, Scheetz, D., Jimenez-Krassel, F., Themmen, A. P. N., Ward, F., Lonergan, P., Smith, G. W., Perez, G. I., Evans, A. C. O., & Ireland, J. J. (2008). Antral Follicle Count Reliably Predicts Number of Morphologically Healthy Oocytes and Follicles in Ovaries of Young Adult Cattle1. Biology of Reproduction, 79(6), 1219–1225. https://doi.org/10.1095/biolreprod.108.071670 Ireland, J, Smith, G., Scheetz, D., Jimenez-Krassel, F., Folger A, B, J. K., Ireland, J. L. H., Mossa, F., Lonergan, P., & Evans, A. (2011). Does size matter in females? An overview of the impact of the high variation in the ovarian reserve on ovarian function and fertility, utility of anti-Mü llerian hormone as a diagnostic marker for fertility and causes of variation in the ovarian reserve i. Reprod. Fertil. Dev., 23(1), 1–14. https://doi.org/10.1071/RD10226 Ireland, JL, Scheetz, D., Jimenez-Krassel, F., Themmen, A., Ward, F., Lonergan, P., Smith, G., Perez, G., Evans, A., & Ireland, J. (2008). Antral Follicle Count Reliably Predicts Number of Morphologically Healthy Oocytes and Follicles in Ovaries of Young Adult Cattle1. Biology of Reproduction, 79(6), 1219–1225. https://doi.org/10.1095/biolreprod.108.071670 Karkanaki, A., Vosnakis, C., & Panidis, D. (2011). The clinical significance of antimüllerian hormone evaluation in gynecological endocrinology. In Hormones (Vol. 10, Issue 2, pp. 95–103). Hellenic Endocrine Society. https://doi.org/10.14310/horm.2002.1299 La Marca, A., & Volpe, A. (2006). Anti-Mullerian hormone (AMH) in female reproduction: is measurement of circulating AMH a useful tool? Clinical Endocrinology, 64(6), 603–610. https://doi.org/10.1111/j.13652265.2006.02533.x Lucy, M. C., Savio, J. D., Badinga, L., De La Sota, R. L., & Thatcher, W. W. (1992). Factors that affect ovarian follicular dynamics in cattle. In Journal of animal science (Vol. 70, Issue 11, pp. 3615–3626). Oxford Academic. https://doi.org/10.2527/1992.70113615x Maldonado, J., Agudelo, B., & Vasquez, A. (2016). Dinamica folicular en novillas y vacas Bos indicus y Bos taurus. Revision de literatura. Revista Colombiana de Ciencias Pecuarias, 10, 65–75. https://revistas.udea.edu.co/index.php/rccp/article/view/27007 Monniaux, D., Barbey, S., Rico, C., Fabre, S., Gallard, Y., & Larroque, H. (2010). Anti-Müllerian hormone: a predictive marker of embryo production in cattle? Reproduction, Fertility and Development, 22(7), 1083. https://doi.org/10.1071/RD09279 Mossa, F., Walsh, S. W., Butler, S. T., Berry, D. P., Carter, F., Lonergan, P., Smith, G. W., Ireland, J. J., & Evans, A. C. O. (2012). Low numbers of ovarian follicles ≥3mm in diameter are associated with low fertility in dairy cows. Journal of Dairy Science, 95(5), 2355–2361. https://doi.org/10.3168/jds.20114325 Motta, P. A., Ramos, N., González, C., & Rojas, E. (2011). Dinámica folicular en la vida reproductiva de la hembra bovina Follicular dynamics in the reproductive life of female livestock. Vet.Zootec., 5(2), 88–99. https://www.researchgate.net/profile/Pablo_Motta_Delgado/publication/311487 357_Dinamica_folicular_en_la_vida_reproductiva_de_la_hembra_bovina_Folli cular_dynamics_in_the_reproductive_life_of_female_livestock/links/58488da9 08aeda696825e819/Dinamica-folicula Ochoa J.C., Ramírez R.R.A., Piccardi M.B., Bó G.A., Tríbulo R. J. 2009. Influencia de la estación en la producción de embriones en donantes de embriones de raza para carne. VIII Simposio Internacional de Reproducción Bovina, Córdoba, Argentina, 26, 27 y 28 de Septiembre. CD. Pfeiffer, K., Jury, L., Endocrinology, J. L.-D. animal, & 2014, U. (2014). Determination of anti-Müllerian hormone at estrus during a synchronized and a natural bovine estrous cycle. Elsevier. https://www.sciencedirect.com/science/article/pii/S0739724013001331 Rey, R., Bedecarrási, P., Olmedo3, S. B., De Vincentiis, S., Calamera, P., Blanco, A. M., Grinspon, R., Natía Freirei, A., & Ffoné, M. G. B. (2013). Actualizaciones Hormona antimülleriana (AMH) como herramienta diagnóstica en la mujer Antimüllerian hormone as a diagnostic too! in the woman. Revista SAEGRE, XX(11), 12–25. https://ri.conicet.gov.ar/handle/11336/7572 Ribeiro, E. S., Bisinotto, R. S., Lima, F. S., Greco, L. F., Morrison, A., Kumar, A., Thatcher, W. W., & Santos, J. E. P. (2014). Plasma anti-Müllerian hormone in adult dairy cows and associations with fertility. Journal of Dairy Science, 97(11), 6888–6900. https://doi.org/10.3168/jds.2014-7908 Rico, C., Fabre, S., Médigue, C., … N. C.-B. of, & 2009, undefined. (2009). AntiMüllerian hormone is an endocrine marker of ovarian gonadotropin-responsive follicles and can help to predict superovulatory responses in the cow. Academic.Oup.Com. https://academic.oup.com/biolreprod/articleabstract/80/1/50/2557561 Rocha Araújo, V., Bezerra Lima-Verde, I., Patrícia, K., Name, O., Báo, S. N., Cabral Campello, C., Roberto, J., Silva, V., Paula, A., Rodrigues, R., & Ricardo De Figueiredo, J. (2010). Bone Morphogenetic Protein-6 (BMP-6) induces atresia in goat primordial follicles cultured in vitro 1. SciELO Brasil, 30(9), 770–776. https://www.scielo.br/scielo.php?pid=S0100736X2010000900010&script=sci_abstract&tlng=es Rojas, J. J., Jubiz, W., & Isaza, C. (2005). Gen SRY y ausencia de tejido testicular en una mujer 47XYY con disgenesia gonadal. In redalyc.org (Vol. 36). EneroMarzo. https://www.redalyc.org/pdf/283/28336107.pdf Sartori S., Sartor-Bergfelt R., Mertens S.A., Guenther J.N., Parrish J.J., Wiltbank M.C. 2002. Fertilization and early embryonic development in heifers and lactating cows in summer and lactating and dry cows in Winter. J Dairy Sci, 85:2803- 2812. Scaramuzzi, R. J., Baird, D. T., Campbell, B. K., Driancourt, M. E., Dupont, J. A., Fortune F, J. E., Gilchrist G, R. B., Martin H, G. B., McNatty I, K. P., McNeilly J, A. S., Monget, P. A., Monniaux, D. A., Viñ oles, C. H., & Webb, R. L. (2011). Regulation of folliculogenesis and the determination of ovulation rate in ruminants. Reproduction, Fertility and Development, 23, 444–467. https://doi.org/10.1071/RD09161 Sunderland, S. J., Crowe, M. A., Boland, M. P., Roche, J. F., & Ireland, J. J. (1994). Selection, dominance and atresia of follicles during the oestrous cycle of heifers. Rep.Bioscientifica.Com, 101(3), 547–555. https://rep.bioscientifica.com/view/journals/rep/101/3/jrf_101_3_006.xml Uribe, F., Zuluaga, A., Valencia, L., & Murgueitio, E. (2011). Buenas prácticas ganaderas. http://up-rid2.up.ac.pa:8080/xmlui/handle/123456789/2120 Vernnuft, A., Schwerhoff, M., Viergutz, T., Diederich, M., & Kuwer, A. (2015). AntiMuellerian hormone levels in plasma of Holstein-Friesian heifers as a predictive parameter for ovum pick-up and embryo production outcomes. Journal of Reproduction and Development, 61(1), 74–79. https://doi.org/10.1262/jrd.2014-091 Vigier, B., Picard, J.-Y., Tran, D., Legeai, L., & Josso, N. (1984). Production of AntiMüllerian Hormone: Another Homology between Sertoli and Granulosa Cells. Endocrinology, 114(4), 1315–1320. https://doi.org/10.1210/endo-114-4-1315 Webb’, R., Nicholas1, B., Gong, J. G., Campbe113, B. K., Gutierrez’, C. G., Garverick5, H. A., & Armstrong’, D. G. (2003). Mechanisms regulating follicular development andd selection of the dominant follicle. In Reproduction Supplement (Vol. 61). http://www.biosciproceedings.org/bp/0005/pdf/bp0005rdr7.pdf Woods, D. C., White, Y. A. R., & Tilly, J. L. (2013). Purification of oogonial stem cells from adult mouse and human ovaries: An assessment of the literature and a view toward the future. In Reproductive Sciences (Vol. 20, Issue 1, pp. 7–15). https://doi.org/10.1177/1933719112462632 Zylbersztein. (2008). La hormona anti Mülleriana.... - Google Académico. Rev Soc Argentina de Endocrinología y Reproducción, 15, 13–28. https://scholar.google.com/scholar?hl=es&as_sdt=0%2C5&q=Zylbersztein%2 C+C.+%282008%29.+La+hormona+anti+Mülleriana.+Su+influencia+en+la+sa lud+reproductiva+de+la+mujer.+Rev+Soc+Argentina+de+Endocrinología+y+R eproducción%2C+15%2C+13-28.&btnG= |
| dc.rights.spa.fl_str_mv |
Derechos Reservados - Universidad de los Llanos, año 2022 |
| dc.rights.uri.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.license.spa.fl_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
Derechos Reservados - Universidad de los Llanos, año 2022 https://creativecommons.org/licenses/by-nc-nd/4.0/ Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.extent.spa.fl_str_mv |
46 páginas |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad de los Llanos |
| dc.publisher.faculty.spa.fl_str_mv |
Facultad de Ciencias Agropecuarias y Recursos Naturales |
| dc.publisher.place.spa.fl_str_mv |
Villavicencio |
| institution |
Universidad de los Llanos |
| bitstream.url.fl_str_mv |
https://dspace7-unillanos.metacatalogo.org/bitstreams/d534cc83-960a-4060-8938-ed3337206fbe/download https://dspace7-unillanos.metacatalogo.org/bitstreams/fde9af5d-0f5f-4503-89fd-0b3d2a6f9732/download https://dspace7-unillanos.metacatalogo.org/bitstreams/340a4cc4-0a4a-4496-abb5-f6188f065131/download https://dspace7-unillanos.metacatalogo.org/bitstreams/d262647e-6b2f-45c8-afc6-cc4199758437/download https://dspace7-unillanos.metacatalogo.org/bitstreams/1871192e-45e6-4896-a7df-57a92d5e62bd/download https://dspace7-unillanos.metacatalogo.org/bitstreams/efd94944-1027-4ca1-9d68-63f76b0d1fd6/download https://dspace7-unillanos.metacatalogo.org/bitstreams/a7af251a-2a5d-43c4-b9fd-f1f9f09230d6/download https://dspace7-unillanos.metacatalogo.org/bitstreams/589dbb94-abf6-41ba-9a7f-700a5bfb0794/download https://dspace7-unillanos.metacatalogo.org/bitstreams/600cc6c9-90a1-4354-bee3-9b5c5939aafe/download https://dspace7-unillanos.metacatalogo.org/bitstreams/ec4ee6d6-ac22-49a5-8ea6-230d28c00b9e/download |
| bitstream.checksum.fl_str_mv |
efe557465f0ce8330ec2e4f417e34cad 984f7190a929d1ebf401c58bf7d8e1ae 5e878960e9156670a556bc82ba4258ae f661acf14bedbf9f5d13897a0387e751 e081f980142f698e1b7292c763cd2322 63436010ebec0f209da11a3221eca485 58a37b873928b69040fb8539130c0649 63333932ba785e37433946a80b54b9ba 89bd078e951081531bcc30ed05000c5a e0eed43be9a81a78d039c690ec67e67f |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Universidad de Los Llanos |
| repository.mail.fl_str_mv |
repositorio@unillanos.edu.co |
| _version_ |
1808490978718253056 |
| spelling |
Góngora Orjuela, Agustín91971a3e6b0453b653040bf65920b520Sierra Martinez, Zully Esmeralda0c7493e989e98e153c5f65a37eeb2579Baquero Morales, Viviana Alejandra0b9bb9e36bb0c412026718b208a11e8d2023-10-23T21:53:39Z2023-10-23T21:53:39Z2022Sierra Martínez, Z. E. y Baquero Morales, V. A. (2022). Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahman [Trabajo de grado, Universidad de los Llanos]. Repositorio digital Universidad de los Llanos.https://repositorio.unillanos.edu.co/handle/001/3107Universidad de los LlanosRepositorio digital Universidad de los Llanoshttps://repositorio.unillanos.edu.co/Incluye tablas y figuras.El éxito de las biotecnologías reproductivas es dependiente de las características fisiológicas del animal, tal como su calidad oocitaria y su reserva ovárica (RO). En vista de lo anterior, las investigaciones en los últimos años han sido enfocadas a determinar marcadores fisiológicos consistentes que puedan predecir la RO en ganado bovino, por esto el objetivo de este estudio es evaluar los niveles séricos de la hormona anti-Mülleriana (AMH) en terneras y novillas Brahman y su relación con la reserva ovárica. En este proyecto se seleccionaron novillas Brahman (n=20), las cuales siendo terneras se les midió los niveles séricos de AMH y se volvieron a medir estos niveles siendo novillas presentando madurez reproductiva. Para evaluar la relación entre los niveles séricos de AMH y su fertilidad, se les realizo un proceso de aspiración folicular para contar el número de folículos antrales mayores de 2 mm en los dos ovarios y la toma de la muestra de sangre. Las concentraciones séricas de AMH se determinaron con un kit inmunoenzimático comercial (AMH Bovina AL-114; Ansh Labs., USA). Las variables utilizadas para relacionar los datos fueron antes y después, es decir cuando eran terneras y luego novillas, por medio del diseño de cohorte sencilla donde se utilizó una distribución normal trabajando una estadística paramétrica utilizando la prueba de Kolmogórov-Smirnov/ Shapiro-Wilk. Se hizo una prueba de T de medidas repetidas para grupos emparejados (antes - después), cada grupo se le realizo un análisis de varianza en su respectiva edad para las concentraciones de AMH (ng/ml) y determinar si había diferencias significativas, se efectuó la agrupación de datos expresada como media ± error estándar y desviación estándar. El nivel de asociación entre los niveles séricos de AMH, la reserva ovárica y producción de embriones in vitro se analizaron mediante la prueba de correlación. Se definieron diferencias significativas cuando el valor de p<0.05. Los análisis estadísticos se realizaron utilizando el software SPSS 26.The success of reproductive biotechnologies is dependent on the physiological characteristics of the animal, such as its oocyte quality and its ovarian reserve (OR). In view of the above, research in recent years has been focused on determining consistent physiological markers that can predict OR in cattle, for this reason the objective of this study is to evaluate serum levels of anti-Müllerian hormone (AMH) in Brahman calves and heifers and their relationship with the ovarian reserve. In this project, Brahman heifers (n=20) were selected, which, being calves, had serum AMH levels measured and these levels were measured again being heifers presenting reproductive maturity. To evaluate the relationship between serum AMH levels and fertility, a follicular aspiration process was performed to count the number of antral follicles larger than 2 mm in both ovaries and blood samples were taken. Serum concentrations of AMH were determined with a commercial immunoenzymatic kit (AMH Bovine AL-114; Ansh Labs., USA). The variables used to relate the data were before and after, that is, when they were calves and then heifers, by means of the simple cohort design where a normal distribution was used working a parametric statistic using the KolmogorovSmirnov/Shapiro-Wilk test. A repeated measures T test was performed for paired groups (before - after), each group underwent an analysis of variance at their respective age for AMH concentrations (ng/ml) and determined if there were significant differences, the pooling of data expressed as a mean ± standard error and standard deviation was performed. The level of association between serum AMH levels, ovarian reserve and in vitro embryo production were analyzed using the correlation test. Significant differences were defined when the p<0.05 value. Statistical analyses were performed using SPSS 26 software.Resumen del proyecto. -- Abstract. -- Planteamiento problema. -- Justificación. -- 1. Objetivos. -- 1.1. Objetivo General: . -- 1.2. Objetivos Específicos: . -- 2. Marco Teórico. -- 2.1. Dinámica folicular. -- 2.2. Ovulación. -- 2.3. AMH. -- 2.3.1. La AMH en la foliculogénesis: . -- 2.3.2. AMH en los bovinos. -- 2.3.3. Medición de la AMH. -- 3. Metodología. -- 3.1. Localización. -- 3.2. Material biológico. -- 3.3. Determinación de la AMH en novillas Brahman. -- 3.4. Análisis estadístico. -- 4. Resultados. -- 4.1. Relación entre la concentración de AMH ng/ml entre terneras y novillas. -- 4.2. Relación entre AMH y población de folículos antrales. -- 4.3. Relación entre AMH y blastocistos. -- 4.4. Correlación de Pearson entre las variables concentración sérica de AMH y número de folículos, oocitos fertilizados y blastocistos. -- 5. Discusión. -- 6. Conclusión. -- 7. Bibliografía. -- 8. Anexos. --PregradoMedico(a) Veterinario(a) ZootecnistaMedicina Veterinaria y Zootecnia46 páginasapplication/pdfspaUniversidad de los LlanosFacultad de Ciencias Agropecuarias y Recursos NaturalesVillavicencioDerechos Reservados - Universidad de los Llanos, año 2022https://creativecommons.org/licenses/by-nc-nd/4.0/Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Hormona anti-mülleriana como marcador endocrino predictor de fertilidad en terneras y novillas brahmanTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_7a1fTexthttps://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85Abbas, A. K.; Lichtman, A. H.; Pillai, S. (2012). Técnicas de laboratorio usadas con frecuencia en inmunología. https://scholar.google.com/scholar?hl=es&as_sdt=0%2C5&q=Abbas%2C+A.+K.%3B+Lichtman%2C+A.+H.%3B+Pillai%2C+S.+%282012%29.+Técnicas+d e+laboratorio+usadas+con+frecuencia+&btnG=Al -Chalabi, S. S., Al-Wattar, Y. T., & Algalili, I. M. (2012). Anti-Mullerian Hormone Is a Significant Marker for Male Infertility. In Tikrit Journal of Pharmaceutical Sciences (Vol. 8, Issue 1). https://www.iasj.net/iasj/download/6c40eab0268cfb9dBaruselli, P., Batista, E., & Ferreira, R. (2016). Niveles plasmáticos de hormona anti-mülleriana permite la selección de donadoras con alto potencial de producción de embriones. SPERMOVA, 1(6), 1–13. https://doi.org/10.18548/aspe/0003.01Batista, E. O. S., Macedo, G. G., Sala, R. V., Ortolan, M. D. D. V., Sá Filho, M. F., Del Valle, T. A., Jesus, E. F., Lopes, R. N. V. R., Rennó, F. P., & Baruselli, P. S. (2014). Plasma antimullerian hormone as a predictor of ovarian antral follicular population in bos indicus (nelore) and bos taurus (holstein) heifers. Reproduction in Domestic Animals, 49(3), 448–452. https://doi.org/10.1111/rda.12304Batista, Vieira, L. M., Sá Filho, M. F., Dias, E. A. R., Bayeux, B. M., Accorsi, M. F., Monteiro, F. M., Souza, A. H., Baruselli, P. S., & D’Occhio, M. J. (2016). Ovarian follicular growth suppression by long-term treatment with a GnRH agonist and impact on small follicle number, oocyte yield, and in vitro embryo production in Zebu beef cows. Theriogenology, 85(9), 1680–1687. https://doi.org/10.1016/j.theriogenology.2016.01.023Batista, EB; Guerreiro, BG; Freitas, JC; Silva, LM; Vieira, RM; Ferreira, RG; Resende, AC; Basso, RN; Lopes, FP; Rennó, AH; Souza; Baruselli, PS. 2015. Plasma anti-Mullerian hormone as predictive endocrine marker for selection of Bos taurus (Holstein) and Bos indicus (Nelore) calves to in vitro embryo production. Submitted for publication.Bezard, J., Vigier, B., Tran, D., Mauleon, P., & Josso, N. (1987). Immunocytochemical study of anti-Mullerian hormone in sheep ovarian follicles during fetal and post-natal development. Reproduction, 80(2), 509–516. https://doi.org/10.1530/jrf.0.0800509Block, E. (1952). Quantitative morphological investigations of the follicular system in women. Cells Tissues Organs, 14(1–2), 108–123. https://doi.org/10.1159/000140595Borges, Á. M., Torres, C. A. A., Ruas, J. R. M., Rocha, V. R., & Carvalho, G. R. (2001). Ovarian follicular dynamics in crossbred Holstein-Zebu heifers. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 53(5), 595–604. https://doi.org/10.1590/s0102-09352001000500015Bustillo, J., & Melo, J. (2020). Parámetros reproductivos y eficiencia reproductiva en ganado bovino reproductive parameters and reproductive efficiency in cattle.Casas, E., & Tewolde, A. (2001). Evaluación de características relacionadas con la eficiencia reproductiva de genotipos criollos de carne en el trópico húmedo.Climent Peris, S. (2013). Embriología y anatomía veterinaria. In Embriología y anatomía veterinaria. http://www.sidalc.net/cgibin/wxis.exe/?IsisScript=librosnl.xis&method=post&formato=2&cantidad=1&expresion=mfn=002490Crowe, M. A., Goulding, D., Baguisi, A., Boland, M. P., & Roche, J. F. (1993). Induced ovulation of the first postpartum dominant follicle in beef suckler cows using a GnRH analogue. Journal of Reproduction and Fertility, 99(2), 551–555. https://doi.org/10.1530/jrf.0.0990551Church, D. 1993. The Ruminant Animal: Digestive Physiology and Nutrition Corvallis, Zaragoza (España). pp. 525-537.Diskin, M. G., Austin, E. J., & Roche, J. F. (2002). Exogenous hormonal manipulation of ovarian activity in cattle. In Domestic Animal Endocrinology (Vol. 23). https://www.sciencedirect.com/science/article/pii/S0739724002001583Durlinger, A., Visser, J., & Themmen, A. (2002). Regulation of ovarian function: the role of anti-Mullerian hormone. In Reproduction.Erickson, B. H. (1966). DEVELOPMENT AND RADIO-RESPONSE OF THE PRENATAL BOVINE OVARY. In Reproducción. https://rep.bioscientifica.com/view/journals/rep/11/1/jrf_11_1_011.xmlFilipiak Y, Viqueira M, B. A. (2016). Desarrollo y dinámica de los folículos ováricos desde la etapa fetal hasta la prepuberal en bovinos. Desarrollo y Dinámica de Los Folículos Ováricos Desde La Etapa Fetal Hasta La Prepuberal En Bovinos, 14–22.FJ, G.-G., AS, S.-R., & J., A.-B. (2004). El factor de crecimiento transformante beta como blanco terapéutico. In Salud Publica Mex (Vol. 46). http://www.insp.mx/salud/index.htmlFortune, J. (2003). The early stages of follicular development: activation of primordial follicles and growth of preantral follicles. Animal Reproduction, 78((3-4)), 135–163. https://www.sciencedirect.com/science/article/pii/S0378432003000885Gan, S. D., & Patel, K. R. (2013). Enzyme Immunoassay and Enzyme-Linked Immunosorbent Assay. Article in Journal of Investigative Dermatology, 12. https://doi.org/10.1038/jid.2013.287García, G. A., Maldonado-Estrada, J. G., & López, J. G. (2003). Caracterización productiva y reproductiva de las explotaciones ganaderas del bajo cauca y el litoral atlántico antioqueños. II. Comportamiento de cuatro grupos. Revista Colombiana de Ciencias Pecuarias, 16(2), 117–125. https://www.redalyc.org/pdf/2950/295026126002.pdfGonzalez, K. (2017). La Raza de Ganado Brahman.Guerreiro, B., Batista, E., … L. V.-D. animal, & 2014, U. (2014). Plasma anti-mullerian hormone: an endocrine marker for in vitro embryo production from Bos taurus and Bos indicus donors. Elsevier. https://www.sciencedirect.com/science/article/pii/S0739724014000678Hasler J.F. 2013. Forty years of embryo transfer in cattle: A review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces. Theriogenology. Vol;81(1,1):152–169.ICA. (2020). Censo pecuario nacional del ICA 2020. https://www.ica.gov.co/areas/pecuaria/servicios/epidemiologia-veterinaria/censos2016/censo-2018Ireland, J, Scheetz, D., Jimenez-Krassel, F., Themmen, A. P. N., Ward, F., Lonergan, P., Smith, G. W., Perez, G. I., Evans, A. C. O., & Ireland, J. J. (2008). Antral Follicle Count Reliably Predicts Number of Morphologically Healthy Oocytes and Follicles in Ovaries of Young Adult Cattle1. Biology of Reproduction, 79(6), 1219–1225. https://doi.org/10.1095/biolreprod.108.071670Ireland, J, Smith, G., Scheetz, D., Jimenez-Krassel, F., Folger A, B, J. K., Ireland, J. L. H., Mossa, F., Lonergan, P., & Evans, A. (2011). Does size matter in females? An overview of the impact of the high variation in the ovarian reserve on ovarian function and fertility, utility of anti-Mü llerian hormone as a diagnostic marker for fertility and causes of variation in the ovarian reserve i. Reprod. Fertil. Dev., 23(1), 1–14. https://doi.org/10.1071/RD10226Ireland, JL, Scheetz, D., Jimenez-Krassel, F., Themmen, A., Ward, F., Lonergan, P., Smith, G., Perez, G., Evans, A., & Ireland, J. (2008). Antral Follicle Count Reliably Predicts Number of Morphologically Healthy Oocytes and Follicles in Ovaries of Young Adult Cattle1. Biology of Reproduction, 79(6), 1219–1225. https://doi.org/10.1095/biolreprod.108.071670Karkanaki, A., Vosnakis, C., & Panidis, D. (2011). The clinical significance of antimüllerian hormone evaluation in gynecological endocrinology. In Hormones (Vol. 10, Issue 2, pp. 95–103). Hellenic Endocrine Society. https://doi.org/10.14310/horm.2002.1299La Marca, A., & Volpe, A. (2006). Anti-Mullerian hormone (AMH) in female reproduction: is measurement of circulating AMH a useful tool? Clinical Endocrinology, 64(6), 603–610. https://doi.org/10.1111/j.13652265.2006.02533.xLucy, M. C., Savio, J. D., Badinga, L., De La Sota, R. L., & Thatcher, W. W. (1992). Factors that affect ovarian follicular dynamics in cattle. In Journal of animal science (Vol. 70, Issue 11, pp. 3615–3626). Oxford Academic. https://doi.org/10.2527/1992.70113615xMaldonado, J., Agudelo, B., & Vasquez, A. (2016). Dinamica folicular en novillas y vacas Bos indicus y Bos taurus. Revision de literatura. Revista Colombiana de Ciencias Pecuarias, 10, 65–75. https://revistas.udea.edu.co/index.php/rccp/article/view/27007Monniaux, D., Barbey, S., Rico, C., Fabre, S., Gallard, Y., & Larroque, H. (2010). Anti-Müllerian hormone: a predictive marker of embryo production in cattle? Reproduction, Fertility and Development, 22(7), 1083. https://doi.org/10.1071/RD09279Mossa, F., Walsh, S. W., Butler, S. T., Berry, D. P., Carter, F., Lonergan, P., Smith, G. W., Ireland, J. J., & Evans, A. C. O. (2012). Low numbers of ovarian follicles ≥3mm in diameter are associated with low fertility in dairy cows. Journal of Dairy Science, 95(5), 2355–2361. https://doi.org/10.3168/jds.20114325Motta, P. A., Ramos, N., González, C., & Rojas, E. (2011). Dinámica folicular en la vida reproductiva de la hembra bovina Follicular dynamics in the reproductive life of female livestock. Vet.Zootec., 5(2), 88–99. https://www.researchgate.net/profile/Pablo_Motta_Delgado/publication/311487 357_Dinamica_folicular_en_la_vida_reproductiva_de_la_hembra_bovina_Folli cular_dynamics_in_the_reproductive_life_of_female_livestock/links/58488da9 08aeda696825e819/Dinamica-foliculaOchoa J.C., Ramírez R.R.A., Piccardi M.B., Bó G.A., Tríbulo R. J. 2009. Influencia de la estación en la producción de embriones en donantes de embriones de raza para carne. VIII Simposio Internacional de Reproducción Bovina, Córdoba, Argentina, 26, 27 y 28 de Septiembre. CD.Pfeiffer, K., Jury, L., Endocrinology, J. L.-D. animal, & 2014, U. (2014). Determination of anti-Müllerian hormone at estrus during a synchronized and a natural bovine estrous cycle. Elsevier. https://www.sciencedirect.com/science/article/pii/S0739724013001331Rey, R., Bedecarrási, P., Olmedo3, S. B., De Vincentiis, S., Calamera, P., Blanco, A. M., Grinspon, R., Natía Freirei, A., & Ffoné, M. G. B. (2013). Actualizaciones Hormona antimülleriana (AMH) como herramienta diagnóstica en la mujer Antimüllerian hormone as a diagnostic too! in the woman. Revista SAEGRE, XX(11), 12–25. https://ri.conicet.gov.ar/handle/11336/7572Ribeiro, E. S., Bisinotto, R. S., Lima, F. S., Greco, L. F., Morrison, A., Kumar, A., Thatcher, W. W., & Santos, J. E. P. (2014). Plasma anti-Müllerian hormone in adult dairy cows and associations with fertility. Journal of Dairy Science, 97(11), 6888–6900. https://doi.org/10.3168/jds.2014-7908Rico, C., Fabre, S., Médigue, C., … N. C.-B. of, & 2009, undefined. (2009). AntiMüllerian hormone is an endocrine marker of ovarian gonadotropin-responsive follicles and can help to predict superovulatory responses in the cow. Academic.Oup.Com. https://academic.oup.com/biolreprod/articleabstract/80/1/50/2557561Rocha Araújo, V., Bezerra Lima-Verde, I., Patrícia, K., Name, O., Báo, S. N., Cabral Campello, C., Roberto, J., Silva, V., Paula, A., Rodrigues, R., & Ricardo De Figueiredo, J. (2010). Bone Morphogenetic Protein-6 (BMP-6) induces atresia in goat primordial follicles cultured in vitro 1. SciELO Brasil, 30(9), 770–776. https://www.scielo.br/scielo.php?pid=S0100736X2010000900010&script=sci_abstract&tlng=esRojas, J. J., Jubiz, W., & Isaza, C. (2005). Gen SRY y ausencia de tejido testicular en una mujer 47XYY con disgenesia gonadal. In redalyc.org (Vol. 36). EneroMarzo. https://www.redalyc.org/pdf/283/28336107.pdfSartori S., Sartor-Bergfelt R., Mertens S.A., Guenther J.N., Parrish J.J., Wiltbank M.C. 2002. Fertilization and early embryonic development in heifers and lactating cows in summer and lactating and dry cows in Winter. J Dairy Sci, 85:2803- 2812.Scaramuzzi, R. J., Baird, D. T., Campbell, B. K., Driancourt, M. E., Dupont, J. A., Fortune F, J. E., Gilchrist G, R. B., Martin H, G. B., McNatty I, K. P., McNeilly J, A. S., Monget, P. A., Monniaux, D. A., Viñ oles, C. H., & Webb, R. L. (2011). Regulation of folliculogenesis and the determination of ovulation rate in ruminants. Reproduction, Fertility and Development, 23, 444–467. https://doi.org/10.1071/RD09161Sunderland, S. J., Crowe, M. A., Boland, M. P., Roche, J. F., & Ireland, J. J. (1994). Selection, dominance and atresia of follicles during the oestrous cycle of heifers. Rep.Bioscientifica.Com, 101(3), 547–555. https://rep.bioscientifica.com/view/journals/rep/101/3/jrf_101_3_006.xmlUribe, F., Zuluaga, A., Valencia, L., & Murgueitio, E. (2011). Buenas prácticas ganaderas. http://up-rid2.up.ac.pa:8080/xmlui/handle/123456789/2120Vernnuft, A., Schwerhoff, M., Viergutz, T., Diederich, M., & Kuwer, A. (2015). AntiMuellerian hormone levels in plasma of Holstein-Friesian heifers as a predictive parameter for ovum pick-up and embryo production outcomes. Journal of Reproduction and Development, 61(1), 74–79. https://doi.org/10.1262/jrd.2014-091Vigier, B., Picard, J.-Y., Tran, D., Legeai, L., & Josso, N. (1984). Production of AntiMüllerian Hormone: Another Homology between Sertoli and Granulosa Cells. Endocrinology, 114(4), 1315–1320. https://doi.org/10.1210/endo-114-4-1315Webb’, R., Nicholas1, B., Gong, J. G., Campbe113, B. K., Gutierrez’, C. G., Garverick5, H. A., & Armstrong’, D. G. (2003). Mechanisms regulating follicular development andd selection of the dominant follicle. In Reproduction Supplement (Vol. 61). http://www.biosciproceedings.org/bp/0005/pdf/bp0005rdr7.pdfWoods, D. C., White, Y. A. R., & Tilly, J. L. (2013). Purification of oogonial stem cells from adult mouse and human ovaries: An assessment of the literature and a view toward the future. In Reproductive Sciences (Vol. 20, Issue 1, pp. 7–15). https://doi.org/10.1177/1933719112462632Zylbersztein. (2008). La hormona anti Mülleriana.... - Google Académico. Rev Soc Argentina de Endocrinología y Reproducción, 15, 13–28. https://scholar.google.com/scholar?hl=es&as_sdt=0%2C5&q=Zylbersztein%2 C+C.+%282008%29.+La+hormona+anti+Mülleriana.+Su+influencia+en+la+sa lud+reproductiva+de+la+mujer.+Rev+Soc+Argentina+de+Endocrinología+y+R eproducción%2C+15%2C+13-28.&btnG=Biotecnologíabovinoscebúmarcador endocrinoreproducciónBiotechnologycattlezebuendocrine markerreproductionPublicationORIGINALINFORME FINAL TESIS ZULLY SIERRA Y VIVIANA BAQUERO 2022.pdfINFORME FINAL TESIS ZULLY SIERRA Y VIVIANA BAQUERO 2022.pdftrabajo para publicar sin firmasapplication/pdf1708739https://dspace7-unillanos.metacatalogo.org/bitstreams/d534cc83-960a-4060-8938-ed3337206fbe/downloadefe557465f0ce8330ec2e4f417e34cadMD51FO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 viviana.pdfFO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 viviana.pdfformato autorizacion Drch vapplication/pdf386873https://dspace7-unillanos.metacatalogo.org/bitstreams/fde9af5d-0f5f-4503-89fd-0b3d2a6f9732/download984f7190a929d1ebf401c58bf7d8e1aeMD52FO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 Zully S.pdfFO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 Zully S.pdfformato autorizacion Drch zapplication/pdf396490https://dspace7-unillanos.metacatalogo.org/bitstreams/340a4cc4-0a4a-4496-abb5-f6188f065131/download5e878960e9156670a556bc82ba4258aeMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-814775https://dspace7-unillanos.metacatalogo.org/bitstreams/d262647e-6b2f-45c8-afc6-cc4199758437/downloadf661acf14bedbf9f5d13897a0387e751MD54TEXTINFORME FINAL TESIS ZULLY SIERRA Y VIVIANA BAQUERO 2022.pdf.txtINFORME FINAL TESIS ZULLY SIERRA Y VIVIANA BAQUERO 2022.pdf.txtExtracted texttext/plain72117https://dspace7-unillanos.metacatalogo.org/bitstreams/1871192e-45e6-4896-a7df-57a92d5e62bd/downloade081f980142f698e1b7292c763cd2322MD55FO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 viviana.pdf.txtFO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 viviana.pdf.txtExtracted texttext/plain2180https://dspace7-unillanos.metacatalogo.org/bitstreams/efd94944-1027-4ca1-9d68-63f76b0d1fd6/download63436010ebec0f209da11a3221eca485MD57FO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 Zully S.pdf.txtFO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 Zully S.pdf.txtExtracted texttext/plain2179https://dspace7-unillanos.metacatalogo.org/bitstreams/a7af251a-2a5d-43c4-b9fd-f1f9f09230d6/download58a37b873928b69040fb8539130c0649MD59THUMBNAILINFORME FINAL TESIS ZULLY SIERRA Y VIVIANA BAQUERO 2022.pdf.jpgINFORME FINAL TESIS ZULLY SIERRA Y VIVIANA BAQUERO 2022.pdf.jpgGenerated Thumbnailimage/jpeg10121https://dspace7-unillanos.metacatalogo.org/bitstreams/589dbb94-abf6-41ba-9a7f-700a5bfb0794/download63333932ba785e37433946a80b54b9baMD56FO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 viviana.pdf.jpgFO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 viviana.pdf.jpgGenerated Thumbnailimage/jpeg13785https://dspace7-unillanos.metacatalogo.org/bitstreams/600cc6c9-90a1-4354-bee3-9b5c5939aafe/download89bd078e951081531bcc30ed05000c5aMD58FO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 Zully S.pdf.jpgFO-DOC-97 FORMATO AUTORIZACION DERECHOS -2019 Zully S.pdf.jpgGenerated Thumbnailimage/jpeg13852https://dspace7-unillanos.metacatalogo.org/bitstreams/ec4ee6d6-ac22-49a5-8ea6-230d28c00b9e/downloade0eed43be9a81a78d039c690ec67e67fMD510001/3107oai:dspace7-unillanos.metacatalogo.org:001/31072024-04-17 16:38:03.483https://creativecommons.org/licenses/by-nc-nd/4.0/Derechos Reservados - Universidad de los Llanos, año 2022open.accesshttps://dspace7-unillanos.metacatalogo.orgRepositorio Universidad de Los Llanosrepositorio@unillanos.edu.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 |