Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental

Devido ao seu rápido crescimento, o feto é particularmente vulnerável a insultos e modificações no millieu hormonal. Este fato sugere que situações adversas experimentadas pela mãe grávida podem alterar o desenvolvimento e a saúde da prole, explicado principalmente pela permeabilidade da barreira pl...

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Autores:: Gonçalves Jr, Vagner
Ligeiro de Oliveira, Ana Paula
Kieling, Karin

Tipo de recurso:: Article of journal

Fecha de publicación:: 2016

Institución:: Universidad de los Llanos

Repositorio:: Repositorio Digital Universidad de los LLanos

Idioma:: spa

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network_name_str	Repositorio Digital Universidad de los LLanos
repository_id_str
dc.title.spa.fl_str_mv	Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental
dc.title.translated.eng.fl_str_mv	Effect of prenatal stress in regulating pulmonary allergic inflammation in a murine model of experimental asthma
title	Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental
spellingShingle	Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental neuroimmunomodulation prenatal stress asthma
title_short	Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental
title_full	Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental
title_fullStr	Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental
title_full_unstemmed	Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental
title_sort	Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental
dc.creator.fl_str_mv	Gonçalves Jr, Vagner Ligeiro de Oliveira, Ana Paula Kieling, Karin
dc.contributor.author.spa.fl_str_mv	Gonçalves Jr, Vagner Ligeiro de Oliveira, Ana Paula Kieling, Karin
dc.subject.eng.fl_str_mv	neuroimmunomodulation prenatal stress asthma
topic	neuroimmunomodulation prenatal stress asthma
description	Devido ao seu rápido crescimento, o feto é particularmente vulnerável a insultos e modificações no millieu hormonal. Este fato sugere que situações adversas experimentadas pela mãe grávida podem alterar o desenvolvimento e a saúde da prole, explicado principalmente pela permeabilidade da barreira placentária a diversos hormônios e substâncias. O objetivo deste trabalho foi estudar o efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar, empregando o modelo murino de asma experimental. Para este propósito foram utilizadas camundongas virgens da linhagem Swiss, com 50 dias de idade. Foi empregado o modelo de choque nas patas para promover o estresse pré-natal e o modelo do “metrô de Nova Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental 65 Iorque” para o estresse pós-natal. As fêmeas foram distribuídas em 4 grupos experimentais: CC: fêmeas não estressadas;CE: fêmeas estressadas pós-natalmente aos 60 dias de idade (PND60), EC: fêmeas nascidas de mães estressadas entre o dia 15 (GD15) e 18 de gestação (GD18); EE: fêmeas nascidas de mães estressadas entre o GD15 e GD18 e estressadas pós-natalmente aos PND60. A indução da inflamação alérgica pulmonar foi realizada através da sensibilização dos animais com solução de ovalbumina (OVA) 0,1 mg.Kg-1 sc para avaliação do leucograma, lavado broncoalveolar (BAL), celularidade hematopoiética medular e neuroquímica. Os experimentos foram realizados 24h após a última sessão de nebulização.O número de células do BAL foi significantemente maior nos animais do grupo EE, em relação àqueles dos grupos CC (P&lt;0.01), CE (P&lt;0.01) e EC (P&lt;0.001). Na contagem diferencial do BAL os linfócitos e macrófagos do grupo EE foram significantemente maiores que em os outros grupos avaliados (P&lt;0.05). Na contagem diferencial de células no sangue não foram observadas alterações (P&gt;0.05) para os linfócitos, neutrófilos, eosinófilos e monócitos; porém, observou-se diferenças significativas (P&lt;0.05) entre o número de bastonetes dos grupos, sendo maior nos animais do grupo CC em relação àquelesdo grupo EC. O número de células hematopoiéticas da medula óssea foi significantemente (P&lt;0.05) menor nos animais do grupo EE, em relação àqueles do grupo CC. No córtex pré-frontal, há diferenças significantes na relação Ácido Homovanílico/Dopamina (HVA/DA) (P&lt;0.05), sendo maior nos animais do grupo EC, em relação àqueles do grupo CE. Em conclusão,o estresse pré-natal levou a modulação de células do sistema imune (SI) dos neonatos, evidenciado após a exposição a estresse agudo pós-natal, amplificando a resposta alérgica pulmonar. Sugere-se que a maior susceptibilidade dos animais do grupo EE seja consequência de alterações induzidas pelo estresse pré-natal no eixo hipotálamo-pituitária-adrenal (HPA).
publishDate	2016
dc.date.accessioned.none.fl_str_mv	2016-07-01T00:00:00Z 2024-07-25T18:14:25Z
dc.date.available.none.fl_str_mv	2016-07-01T00:00:00Z 2024-07-25T18:14:25Z
dc.date.issued.none.fl_str_mv	2016-07-01
dc.type.spa.fl_str_mv	Artículo de revista
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dc.type.local.eng.fl_str_mv	Journal article
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/publishedVersion
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dc.identifier.issn.none.fl_str_mv	0121-3709
dc.identifier.uri.none.fl_str_mv	https://repositorio.unillanos.edu.co/handle/001/3862
dc.identifier.doi.none.fl_str_mv	10.22579/20112629.364
dc.identifier.eissn.none.fl_str_mv	2011-2629
dc.identifier.url.none.fl_str_mv	https://doi.org/10.22579/20112629.364
identifier_str_mv	0121-3709 10.22579/20112629.364 2011-2629
url	https://repositorio.unillanos.edu.co/handle/001/3862 https://doi.org/10.22579/20112629.364
dc.language.iso.spa.fl_str_mv	spa
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Del Prete G. Human Th1 and Th2 lymphocytes: their role in the pathophysiology of atopy. Allergy. 1992;47(5):450–455. Riffo-Vasquez Y, A. P. Ligeiro de Oliveira AP, Page CP, Spina D, Tavares- de-Lima W. Role of sex hormones in allergic inflammation in mice. Clinical and Experimental Allergy. 2007;37(3):459–470. Saal FS, Even MD, Quadagno DM. Effects of maternal stress on puberty, fertility and aggressive behavior of female mice from different intrauterine positions. Physiology & Behavior. 1991;49(6):1073–1078. Segerstrom SC. Psychological Stress and the Human Immune System: A Meta- Analytic Study of 30 Years of Inquiry. Psychol ogy Bulletin. 2004;130(4):601–630. Semple BD, Blomgren K, Gimlin K, Ferriero DM, Noble-Haeusslein LJ. Brain development in rodents and humans: Identifying benchmarks of maturation and vulnerability to injury across species. Progress in Neurobiology. 2013;106(107):1–16. Sternberg EM. Eurosterone meeting. 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spelling	Gonçalves Jr, VagnerLigeiro de Oliveira, Ana PaulaKieling, Karin2016-07-01T00:00:00Z2024-07-25T18:14:25Z2016-07-01T00:00:00Z2024-07-25T18:14:25Z2016-07-010121-3709https://repositorio.unillanos.edu.co/handle/001/386210.22579/20112629.3642011-2629https://doi.org/10.22579/20112629.364Devido ao seu rápido crescimento, o feto é particularmente vulnerável a insultos e modificações no millieu hormonal. Este fato sugere que situações adversas experimentadas pela mãe grávida podem alterar o desenvolvimento e a saúde da prole, explicado principalmente pela permeabilidade da barreira placentária a diversos hormônios e substâncias. O objetivo deste trabalho foi estudar o efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar, empregando o modelo murino de asma experimental. Para este propósito foram utilizadas camundongas virgens da linhagem Swiss, com 50 dias de idade. Foi empregado o modelo de choque nas patas para promover o estresse pré-natal e o modelo do “metrô de Nova Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental 65 Iorque” para o estresse pós-natal. As fêmeas foram distribuídas em 4 grupos experimentais: CC: fêmeas não estressadas;CE: fêmeas estressadas pós-natalmente aos 60 dias de idade (PND60), EC: fêmeas nascidas de mães estressadas entre o dia 15 (GD15) e 18 de gestação (GD18); EE: fêmeas nascidas de mães estressadas entre o GD15 e GD18 e estressadas pós-natalmente aos PND60. A indução da inflamação alérgica pulmonar foi realizada através da sensibilização dos animais com solução de ovalbumina (OVA) 0,1 mg.Kg-1 sc para avaliação do leucograma, lavado broncoalveolar (BAL), celularidade hematopoiética medular e neuroquímica. Os experimentos foram realizados 24h após a última sessão de nebulização.O número de células do BAL foi significantemente maior nos animais do grupo EE, em relação àqueles dos grupos CC (P&lt;0.01), CE (P&lt;0.01) e EC (P&lt;0.001). Na contagem diferencial do BAL os linfócitos e macrófagos do grupo EE foram significantemente maiores que em os outros grupos avaliados (P&lt;0.05). Na contagem diferencial de células no sangue não foram observadas alterações (P&gt;0.05) para os linfócitos, neutrófilos, eosinófilos e monócitos; porém, observou-se diferenças significativas (P&lt;0.05) entre o número de bastonetes dos grupos, sendo maior nos animais do grupo CC em relação àquelesdo grupo EC. O número de células hematopoiéticas da medula óssea foi significantemente (P&lt;0.05) menor nos animais do grupo EE, em relação àqueles do grupo CC. No córtex pré-frontal, há diferenças significantes na relação Ácido Homovanílico/Dopamina (HVA/DA) (P&lt;0.05), sendo maior nos animais do grupo EC, em relação àqueles do grupo CE. Em conclusão,o estresse pré-natal levou a modulação de células do sistema imune (SI) dos neonatos, evidenciado após a exposição a estresse agudo pós-natal, amplificando a resposta alérgica pulmonar. Sugere-se que a maior susceptibilidade dos animais do grupo EE seja consequência de alterações induzidas pelo estresse pré-natal no eixo hipotálamo-pituitária-adrenal (HPA).Due to the rapid growth of the fetus it is particularly vulnerable to insults and changes in hormonal milieu. Therefore, is suggested that adverse situations experienced by the pregnant mother can alter the development and health of offspring, mainly due to the permeability of the placental barrier to various hormones and substances. The aim of the present investigation was to study the effects of prenatal stress in the regulation of pulmonary allergic inflammation, employing the murine model of experimental asthma. For this purpose, were used virgin female mice, Swiss lineage, of 50 days old. The models used were foot shock to induce prenatally stress, and "New York subway" stress to induce postnatally stress. Females were divided into 4 groups: CC group: not stressed females; CE group: postnatally stressed females (PND60); EC: females born from stressed mothers (GD15 to GD18); EE Group: females born from stressed mothers (GD15 to GD18) (footshock) and postnatally stressed (PND60). The induction of allergic pulmonary inflammation was done through sensitization of animals with 0,1 mg.Kg-1 sc of ovalbumin (OVA) solution, to further evaluate leukogram, bronchoalveolar lavage (BAL) hematopoietic marrow cellularity and neurochemistry. The experiments were performed 24 hours after the last session of nebulization. The number of BAL cells was significantly higher in EE group animals compared with the CC group (P&lt;0.01), CE (P&lt;0.01) and CE (P&lt;0.001). In the differential count of the BAL, lymphocytes and macrophages of EE group were significantly higher than the other groups studied (P&lt;0.05). In the blood differential cell count were not observed changes (P&gt;0.05) for lymphocytes, neutrophils, eosinophils and monocytes; however, there were significant differences (P&lt;0.05) observed in the number of rods cells between groups, being higher in animals the CC group compared to EC group. The number of hematopoietic cells of the bone marrow was significantly lower (P&lt;0.05) in animals of Group EE, compared with CC group. In the prefrontal cortex, there were significant differences in homovanillic acid /dopamine (HVA/DA) (P&lt;0.05) rate, being higher in the EC group, compared to EC group. In conclusion, prenatal stress modulated the immune system (SI) cells of neonates, evidenced after exposure to a post-natal acute stress by amplification of pulmonary allergic response. It is suggested that the increased susceptibility of animals EE group is a result of changes induced by prenatal stress on hypothalamus pituitary-adrenal (HPA) axis.application/pdftext/htmlspaUniversidad de los LlanosOrinoquia - 2017https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/364neuroimmunomodulationprenatal stressasthmaEfeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimentalEffect of prenatal stress in regulating pulmonary allergic inflammation in a murine model of experimental asthmaArtículo de revistainfo:eu-repo/semantics/articleJournal articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Texthttp://purl.org/coar/version/c_970fb48d4fbd8a85Ader R, Cohen N, Felten D. Psychoneuroimmunology: interactions system and the immune system. The Lancet. 1995;345: 99–103.Berkenbosh F, Wolvers D, Derijk R. Stress exposure and immunization Radioimmunoassays (RIAs) CRF antiserum protocol and. Journal of Steroidal Biochemistry and Molecular Biology. 1991;40(4): 639–647.Biondi M, Zannino LG. Psychological Stress, Neuroimmunomodulation, and Susceptibility to Infectious Diseases in Animals and Man: A Review. Psychotherapy and Psychosomatics. 1997;66(1):3–26.Boulet LP, FitzGerald JM, Reddel HK. The revised 2014 GINA strategy report: opportunities for change. Current opinion in pulmonary medicine. 2015;21(1): 1–7.Chen E, Miller GE. Stress and inflammation in exacerbations of asthma. Brain, Behavior, and Immunity. 2007;21(8):993–999.Choy DF, Choy DF, Hart KM, Borthwick LA, Shikotra A, Nagarkar DR, et al. TH2 and TH17 inflammatory pathways are reciprocally regulated in asthma. 2015;7(301):301-.Dhabhar FS, McEwen BS. 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Brain, Behavior and Immunity. 2005;19(4): 296–308.https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/364/pdf_53https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/364/html_207726420OrinoquiaPublicationOREORE.xmltext/xml2715https://repositorio.unillanos.edu.co/bitstreams/0a167f3d-16dd-4d91-9674-bee69d50be3b/downloade04bd06adbfd81e9a9910a5b1d9c0077MD51001/3862oai:repositorio.unillanos.edu.co:001/38622024-07-25 13:14:25.538https://creativecommons.org/licenses/by-nc-sa/4.0/Orinoquia - 2017metadata.onlyhttps://repositorio.unillanos.edu.coRepositorio Universidad de Los Llanosrepositorio@unillanos.edu.co

Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental

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