Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú

El objetivo de este estudio fue determinar la expresión de proteínas del fluido folicular (FF) y su relación con la calidad del oocito. Se evaluaron 52 ovarios de planta de faenado de vacas Cebú comercial, mediante la técnica de disección y aspiración folicular se obtuvo FF y oocitos. Las evaluacion...

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Autores:
Neira-Rivera, Eliana
Gutiérrez, Sonia L.
Castillo-Barón, Lidy V.
Velásquez-Penagos, José G.
Góngora-Orjuela, Agustín
Cardozo-Cerquera, Jaime A.
Tipo de recurso:
Article of journal
Fecha de publicación:
2020
Institución:
Universidad de los Llanos
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Repositorio Digital Universidad de los LLanos
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spa
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oai:repositorio.unillanos.edu.co:001/3980
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https://repositorio.unillanos.edu.co/handle/001/3980
https://doi.org/10.22579/20112629.598
Palabra clave:
bovine follicle
protein band
oocyte
protein profile
Folículo bovino
bandas de proteínas
oocito
perfiles proteícos
Folículo bovino
fluido folicular
oócito
bandas de proteína
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openAccess
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Orinoquia - 2020
id Unillanos2_cb854764c73f6f1984d2f9529ee82f08
oai_identifier_str oai:repositorio.unillanos.edu.co:001/3980
network_acronym_str Unillanos2
network_name_str Repositorio Digital Universidad de los LLanos
repository_id_str
dc.title.spa.fl_str_mv Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
dc.title.translated.eng.fl_str_mv Follicular fluid (FF) protein expression associated with oocyte quality in Zebu cattle
title Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
spellingShingle Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
bovine follicle
protein band
oocyte
protein profile
Folículo bovino
bandas de proteínas
oocito
perfiles proteícos
Folículo bovino
fluido folicular
oócito
bandas de proteína
title_short Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
title_full Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
title_fullStr Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
title_full_unstemmed Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
title_sort Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
dc.creator.fl_str_mv Neira-Rivera, Eliana
Gutiérrez, Sonia L.
Castillo-Barón, Lidy V.
Velásquez-Penagos, José G.
Góngora-Orjuela, Agustín
Cardozo-Cerquera, Jaime A.
dc.contributor.author.spa.fl_str_mv Neira-Rivera, Eliana
Gutiérrez, Sonia L.
Castillo-Barón, Lidy V.
Velásquez-Penagos, José G.
Góngora-Orjuela, Agustín
Cardozo-Cerquera, Jaime A.
dc.subject.eng.fl_str_mv bovine follicle
protein band
oocyte
protein profile
topic bovine follicle
protein band
oocyte
protein profile
Folículo bovino
bandas de proteínas
oocito
perfiles proteícos
Folículo bovino
fluido folicular
oócito
bandas de proteína
dc.subject.spa.fl_str_mv Folículo bovino
bandas de proteínas
oocito
perfiles proteícos
Folículo bovino
fluido folicular
oócito
bandas de proteína
description El objetivo de este estudio fue determinar la expresión de proteínas del fluido folicular (FF) y su relación con la calidad del oocito. Se evaluaron 52 ovarios de planta de faenado de vacas Cebú comercial, mediante la técnica de disección y aspiración folicular se obtuvo FF y oocitos. Las evaluaciones realizadas fueron: calidad del oocito por aspecto citoplasmático y células del cúmulos y perfil de proteínas del FF mediante SDS-PAGE. Se realizó el análisis descriptivo, a través del procedimiento MEANS, análisis de varianza (PROC. ANOVA) y para las diferencias estadísticas significativas se usó la prueba de comparación de Bonferroni con un nivel de significancia del 5%, mediante el paquete estadístico SAS®. El 52% de los oocitos se categorizaron con calidad I-II. El análisis unidimensional de las proteínas del FF evidenció la presencia de 25 bandas de proteína entre 9 y 240 kDa. En folículos <3 mm se expresaron 23 bandas, en folículos de 3 y 6 mm 19 bandas y en folículos >6mm 20 bandas. Las bandas de peso molecular (PM) de 26kDa, 57kDa y 68kDa representan la mayor concentración en el FF; 4 bandas de PM 14 KDa, 34 KDa, 76 y 79 KDa, solo en folículos de <3mm, 2 bandas de PM 9 y 91 KDa solo en folículos de >3 mm. La banda de 32 KDa no se observó en folículos > de 6mm. Las bandas de mayor frecuencia de presentación fueron las de 26, 40, 42, 57, 68, 240 KDa. Las bandas de proteína que se asociaron con la calidad del oocito en forma significativa (p<0,05) fueron las de PM 24, 57, 68 y 164 KDa para FF de folículos <3mm y las bandas de PM 13, 26 y 38 kDa entre 3 y 6mm, y la de 26 kDa a folículos > de 6mm. Los resultados nos indican asociaciones de la calidad del oocito con algunas bandas de proteína.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-05-11T00:00:00Z
2024-07-25T18:15:02Z
dc.date.available.none.fl_str_mv 2020-05-11T00:00:00Z
2024-07-25T18:15:02Z
dc.date.issued.none.fl_str_mv 2020-05-11
dc.type.spa.fl_str_mv Artículo de revista
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dc.type.local.eng.fl_str_mv Journal article
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dc.type.content.spa.fl_str_mv Text
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dc.identifier.doi.none.fl_str_mv 10.22579/20112629.598
dc.identifier.eissn.none.fl_str_mv 2011-2629
dc.identifier.url.none.fl_str_mv https://doi.org/10.22579/20112629.598
identifier_str_mv 0121-3709
10.22579/20112629.598
2011-2629
url https://repositorio.unillanos.edu.co/handle/001/3980
https://doi.org/10.22579/20112629.598
dc.language.iso.spa.fl_str_mv spa
language spa
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Aller JF, Callejas SS, Alberio RH. Biochemical and steroid concentrations in follicular fluid and blood plasma in different follicular waves of the estrous cycle from normal and superovulated beef cows. Anim Reprod Sci, 2013;142:113-120
Ambekar A, Nirujogi R, Srikanth S, Chavan S, Kelkar D, Hinduja I, et al. Proteomic analysis of human follicular fluid: A new perspective towards understanding folliculogenesis. J Proteomics. 2013;87:68-77
Andersen M, Kroll J, Byskov A, Faber M. Protein composition in the fluid of individual bovine follicles. J Reprod Fertil. 1976;48(1):109-118
Angelucci S, Ciavardelli D, Di Giuseppe F, Eleuterio E, Sulpizio M, Tiboni GM, et al. Proteome analysis of human follicular fluid. Biochim Biophys Acta - Proteins Proteomics. 2006;1764(11):1775-1785
Armstrong D, Hogg C, Campbell B, Webb R. Insulin-like growth factor (IGF)-binding protein production by primary cultures of ovine granulosa and theca cells. The effects of IGF-I, gonadotropin, and follicle size. Biol Reprod. 1996;55(5):1163-1171
Austin EJ, Mihm M, Evans ACO, et al. Alterations in Intrafollicular Regulatory Factors and Apoptosis During Selection of Follicles in the First Follicular Wave of the Bovine Estrous Cycle. Biol Reprod. 2001;64:839-848
Bianchi L, Gagliardi A, Campanella G, Landi C, Capaldo A, Carleo A, et al. A methodological and functional proteomic approach of human follicular fluid en route for oocyte. J Proteom. 2013;90:61-76
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Colazo MG, Mapletoft RJ. Fisiologia del ciclo estral bovino. Conference Paper. De Jong G, van Dijk JP, van Eijk HG. 1990. The biology of transferrin. Clin Chim Acta. 2014;190(1-2):1-46
Dekel N. Cellular Biochemical and molecular mechanisms regulating oocyte maturation. Mol cell endocrinol. 2005;235:19-25
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spelling Neira-Rivera, ElianaGutiérrez, Sonia L.Castillo-Barón, Lidy V.Velásquez-Penagos, José G.Góngora-Orjuela, AgustínCardozo-Cerquera, Jaime A.2020-05-11T00:00:00Z2024-07-25T18:15:02Z2020-05-11T00:00:00Z2024-07-25T18:15:02Z2020-05-110121-3709https://repositorio.unillanos.edu.co/handle/001/398010.22579/20112629.5982011-2629https://doi.org/10.22579/20112629.598El objetivo de este estudio fue determinar la expresión de proteínas del fluido folicular (FF) y su relación con la calidad del oocito. Se evaluaron 52 ovarios de planta de faenado de vacas Cebú comercial, mediante la técnica de disección y aspiración folicular se obtuvo FF y oocitos. Las evaluaciones realizadas fueron: calidad del oocito por aspecto citoplasmático y células del cúmulos y perfil de proteínas del FF mediante SDS-PAGE. Se realizó el análisis descriptivo, a través del procedimiento MEANS, análisis de varianza (PROC. ANOVA) y para las diferencias estadísticas significativas se usó la prueba de comparación de Bonferroni con un nivel de significancia del 5%, mediante el paquete estadístico SAS®. El 52% de los oocitos se categorizaron con calidad I-II. El análisis unidimensional de las proteínas del FF evidenció la presencia de 25 bandas de proteína entre 9 y 240 kDa. En folículos <3 mm se expresaron 23 bandas, en folículos de 3 y 6 mm 19 bandas y en folículos >6mm 20 bandas. Las bandas de peso molecular (PM) de 26kDa, 57kDa y 68kDa representan la mayor concentración en el FF; 4 bandas de PM 14 KDa, 34 KDa, 76 y 79 KDa, solo en folículos de <3mm, 2 bandas de PM 9 y 91 KDa solo en folículos de >3 mm. La banda de 32 KDa no se observó en folículos > de 6mm. Las bandas de mayor frecuencia de presentación fueron las de 26, 40, 42, 57, 68, 240 KDa. Las bandas de proteína que se asociaron con la calidad del oocito en forma significativa (p<0,05) fueron las de PM 24, 57, 68 y 164 KDa para FF de folículos <3mm y las bandas de PM 13, 26 y 38 kDa entre 3 y 6mm, y la de 26 kDa a folículos > de 6mm. Los resultados nos indican asociaciones de la calidad del oocito con algunas bandas de proteína.This study was aimed at determining follicular fluid (FF) protein expression and its relationship with oocyte quality. FF and oocytes were obtained by dissection and follicular aspiration of the ovaries from fifty-two commercial Zebu from a slaughterhouse. Oocyte quality was measured by cytoplasmic aspect and cumulus cells and FF protein profile by SDS-PAGE. The SAS statistical package’s PROC MEANS and analysis of variance (ANOVA) were used for descriptive analysis and the Bonferroni comparison test for assessing significant statistical differences (5% significance level); 52% of the oocytes were categorised as having I-II quality. One-dimensional SDS-PAGE analysis of FF proteins revealed 25 protein bands having 9 kDa to 240 kDa molecular weight (MW); 23 bands were expressed in <3 mm follicles, 19 bands in 3 and 6 mm follicles and 20 bands in >6 mm follicles. The 26kDa, 57kDa and 68kDa bands’ MW represented the highest FF concentration whereas only four bands (14 kDa, 34 kDa, 76 and 79 kDa MW) were found in <3mm follicles and only 2 bands (9kDa and 91 kDa MW) in >3 mm follicles. The 32 kDa band was not observed in >6mm follicles. The 26, 40, 42, 57, 68 and 240 KDa bands occurred with the greatest frequency. The protein bands which were significantly associated with oocyte quality (p<0.05) were 24, 57, 68 and 164 kDa MW for <3mm follicles, 13, 26 and 38 kDa MW bands for 3 and 6mm follicles and 26 kDa for >6mm follicles. The results indicated oocyte quality association with some protein bands.application/pdfspaUniversidad de los LlanosOrinoquia - 2020https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/598bovine follicleprotein bandoocyteprotein profileFolículo bovinobandas de proteínasoocitoperfiles proteícosFolículo bovinofluido folicularoócitobandas de proteínaExpresión proteica del fluido folicular asociado a la calidad del oocito de vacas CebúFollicular fluid (FF) protein expression associated with oocyte quality in Zebu cattleArtículo de revistainfo:eu-repo/semantics/articleJournal articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Texthttp://purl.org/coar/version/c_970fb48d4fbd8a85Alavi-Shoushtari S, Asri-Rezai S, Abshenas J. A study of the uterine protein variations during the estrus cycle in the cow: A comparison with the serum proteins. Anim Reprod Sci, 2006;96(1-2):10-20Aller JF, Callejas SS, Alberio RH. Biochemical and steroid concentrations in follicular fluid and blood plasma in different follicular waves of the estrous cycle from normal and superovulated beef cows. Anim Reprod Sci, 2013;142:113-120Ambekar A, Nirujogi R, Srikanth S, Chavan S, Kelkar D, Hinduja I, et al. Proteomic analysis of human follicular fluid: A new perspective towards understanding folliculogenesis. J Proteomics. 2013;87:68-77Andersen M, Kroll J, Byskov A, Faber M. Protein composition in the fluid of individual bovine follicles. J Reprod Fertil. 1976;48(1):109-118Angelucci S, Ciavardelli D, Di Giuseppe F, Eleuterio E, Sulpizio M, Tiboni GM, et al. Proteome analysis of human follicular fluid. Biochim Biophys Acta - Proteins Proteomics. 2006;1764(11):1775-1785Armstrong D, Hogg C, Campbell B, Webb R. 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J Proteomics. 2016;139:122-129https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/598/pdf4113224OrinoquiaPublicationOREORE.xmltext/xml2816https://repositorio.unillanos.edu.co/bitstreams/8e1f4bd2-d56a-4fa2-8014-c1b0a7888021/downloadf7f9908460c0a470503208cb030ef507MD51001/3980oai:repositorio.unillanos.edu.co:001/39802024-07-25 13:15:02.128https://creativecommons.org/licenses/by-nc-sa/4.0/Orinoquia - 2020metadata.onlyhttps://repositorio.unillanos.edu.coRepositorio Universidad de Los Llanosrepositorio@unillanos.edu.co