Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú

El objetivo de este estudio fue determinar la expresión de proteínas del fluido folicular (FF) y su relación con la calidad del oocito. Se evaluaron 52 ovarios de planta de faenado de vacas Cebú comercial, mediante la técnica de disección y aspiración folicular se obtuvo FF y oocitos. Las evaluacion...

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Autores:: Neira-Rivera, Eliana
Gutiérrez, Sonia L.
Castillo-Barón, Lidy V.
Velásquez-Penagos, José G.
Góngora-Orjuela, Agustín
Cardozo-Cerquera, Jaime A.

Tipo de recurso:: Article of journal

Fecha de publicación:: 2020

Institución:: Universidad de los Llanos

Repositorio:: Repositorio Digital Universidad de los LLanos

Idioma:: spa

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network_name_str	Repositorio Digital Universidad de los LLanos
repository_id_str
dc.title.spa.fl_str_mv	Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
dc.title.translated.eng.fl_str_mv	Follicular fluid (FF) protein expression associated with oocyte quality in Zebu cattle
title	Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
spellingShingle	Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú Mineralization petroleum spills soil pollution phytoremediation bioremediation mineralización derrames de petróleo contaminación de suelos fitorremediación biorremediación
title_short	Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
title_full	Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
title_fullStr	Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
title_full_unstemmed	Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
title_sort	Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú
dc.creator.fl_str_mv	Neira-Rivera, Eliana Gutiérrez, Sonia L. Castillo-Barón, Lidy V. Velásquez-Penagos, José G. Góngora-Orjuela, Agustín Cardozo-Cerquera, Jaime A.
dc.contributor.author.spa.fl_str_mv	Neira-Rivera, Eliana Gutiérrez, Sonia L. Castillo-Barón, Lidy V. Velásquez-Penagos, José G. Góngora-Orjuela, Agustín Cardozo-Cerquera, Jaime A.
dc.subject.eng.fl_str_mv	Mineralization petroleum spills soil pollution phytoremediation bioremediation
topic	Mineralization petroleum spills soil pollution phytoremediation bioremediation mineralización derrames de petróleo contaminación de suelos fitorremediación biorremediación
dc.subject.spa.fl_str_mv	mineralización derrames de petróleo contaminación de suelos fitorremediación biorremediación
description	El objetivo de este estudio fue determinar la expresión de proteínas del fluido folicular (FF) y su relación con la calidad del oocito. Se evaluaron 52 ovarios de planta de faenado de vacas Cebú comercial, mediante la técnica de disección y aspiración folicular se obtuvo FF y oocitos. Las evaluaciones realizadas fueron: calidad del oocito por aspecto citoplasmático y células del cúmulos y perfil de proteínas del FF mediante SDS-PAGE. Se realizó el análisis descriptivo, a través del procedimiento MEANS, análisis de varianza (PROC. ANOVA) y para las diferencias estadísticas significativas se usó la prueba de comparación de Bonferroni con un nivel de significancia del 5%, mediante el paquete estadístico SAS®. El 52% de los oocitos se categorizaron con calidad I-II. El análisis unidimensional de las proteínas del FF evidenció la presencia de 25 bandas de proteína entre 9 y 240 kDa. En folículos &lt;3 mm se expresaron 23 bandas, en folículos de 3 y 6 mm 19 bandas y en folículos &gt;6mm 20 bandas. Las bandas de peso molecular (PM) de 26kDa, 57kDa y 68kDa representan la mayor concentración en el FF; 4 bandas de PM 14 KDa, 34 KDa, 76 y 79 KDa, solo en folículos de &lt;3mm, 2 bandas de PM 9 y 91 KDa solo en folículos de &gt;3 mm. La banda de 32 KDa no se observó en folículos &gt; de 6mm. Las bandas de mayor frecuencia de presentación fueron las de 26, 40, 42, 57, 68, 240 KDa. Las bandas de proteína que se asociaron con la calidad del oocito en forma significativa (p&lt;0,05) fueron las de PM 24, 57, 68 y 164 KDa para FF de folículos &lt;3mm y las bandas de PM 13, 26 y 38 kDa entre 3 y 6mm, y la de 26 kDa a folículos &gt; de 6mm. Los resultados nos indican asociaciones de la calidad del oocito con algunas bandas de proteína.
publishDate	2020
dc.date.accessioned.none.fl_str_mv	2020-05-11 00:00:00 2022-06-13T17:42:39Z
dc.date.available.none.fl_str_mv	2020-05-11 00:00:00 2022-06-13T17:42:39Z
dc.date.issued.none.fl_str_mv	2020-05-11
dc.type.spa.fl_str_mv	Artículo de revista
dc.type.eng.fl_str_mv	Journal Article
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dc.type.local.spa.fl_str_mv	Sección Ciencias agrarias
dc.type.local.eng.fl_str_mv	Sección Agricultural sciences
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/publishedVersion
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dc.type.content.spa.fl_str_mv	Text
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dc.identifier.issn.none.fl_str_mv	0121-3709
dc.identifier.uri.none.fl_str_mv	https://repositorio.unillanos.edu.co/handle/001/2749
dc.identifier.doi.none.fl_str_mv	10.22579/20112629.598
dc.identifier.eissn.none.fl_str_mv	2011-2629
dc.identifier.url.none.fl_str_mv	https://doi.org/10.22579/20112629.598
identifier_str_mv	0121-3709 10.22579/20112629.598 2011-2629
url	https://repositorio.unillanos.edu.co/handle/001/2749 https://doi.org/10.22579/20112629.598
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.references.spa.fl_str_mv	Alavi-Shoushtari S, Asri-Rezai S, Abshenas J. A study of the uterine protein variations during the estrus cycle in the cow: A comparison with the serum proteins. Anim Reprod Sci, 2006;96(1-2):10-20 Aller JF, Callejas SS, Alberio RH. Biochemical and steroid concentrations in follicular fluid and blood plasma in different follicular waves of the estrous cycle from normal and superovulated beef cows. Anim Reprod Sci, 2013;142:113-120 Ambekar A, Nirujogi R, Srikanth S, Chavan S, Kelkar D, Hinduja I, et al. Proteomic analysis of human follicular fluid: A new perspective towards understanding folliculogenesis. J Proteomics. 2013;87:68-77 Andersen M, Kroll J, Byskov A, Faber M. Protein composition in the fluid of individual bovine follicles. J Reprod Fertil. 1976;48(1):109-118 Angelucci S, Ciavardelli D, Di Giuseppe F, Eleuterio E, Sulpizio M, Tiboni GM, et al. Proteome analysis of human follicular fluid. Biochim Biophys Acta - Proteins Proteomics. 2006;1764(11):1775-1785 Armstrong D, Hogg C, Campbell B, Webb R. Insulin-like growth factor (IGF)-binding protein production by primary cultures of ovine granulosa and theca cells. The effects of IGF-I, gonadotropin, and follicle size. Biol Reprod. 1996;55(5):1163-1171 Austin EJ, Mihm M, Evans ACO, et al. Alterations in Intrafollicular Regulatory Factors and Apoptosis During Selection of Follicles in the First Follicular Wave of the Bovine Estrous Cycle. Biol Reprod. 2001;64:839-848 Bianchi L, Gagliardi A, Campanella G, Landi C, Capaldo A, Carleo A, et al. A methodological and functional proteomic approach of human follicular fluid en route for oocyte. J Proteom. 2013;90:61-76 Bijttebier J, Tilleman K, et al. Comparative proteome analysis of porcine follicular fluid and serum reveals that excessive alpha (2)-macroglobulin in serum hampers successful expansion of cumulus-oocyte complexes. Proteomics, 2009;9:4554- 4565 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254 Briggs D, Sharp D, Miller D, Gosden R. Transferrin in the developing ovarian follicle: evidence for de-novo expression by granulosa cells. Mol Hum Reprod; 1999;5(12):1107-1114 Clarke H, Hope S, Byers S, Rodgers RJ. Formation of ovarian follicular fluid may be due to the osmotic potential of large glycosaminoglycans and proteoglycans. Reprod. 2006;132(1):119-131 Colazo MG, Mapletoft RJ. Fisiologia del ciclo estral bovino. Conference Paper. De Jong G, van Dijk JP, van Eijk HG. 1990. The biology of transferrin. Clin Chim Acta. 2014;190(1-2):1-46 Dekel N. Cellular Biochemical and molecular mechanisms regulating oocyte maturation. Mol cell endocrinol. 2005;235:19-25 Driancourt MA. Regulation of ovarian follicular dynamics in farm animals. Implications for manipulation of reproduction. Theriogenology. 2001;55(6):1211-1239 Dumont J, Umbhauer M, et al. p90Rsk is not involved in cytostatic factor arrest in mouse oocytes. J Cell Biol. 2005;169:227-231 Fahiminiya S, Reynaud K, Labas V, Batard S, Chastant-Maillard S, Gérard N. Steroid hormones content and proteomic analysis of canine follicular fluid during the preovulatory period. Reprod Biol. 2010;8(1):132 Fahiminiya S, Labas V, Roche S, Dacheux J, Gérard N. Proteomic analysis of mare follicular fluid during late follicle development. Proteome Sci. 2011;9:54 Fortune JE, Hansel W. Concentrations of steroids andgonadotroipns in follicular fluid from normal heifers and heifersprimed for superovulation. Biol Reprod. 1985;32:1069-1079 Fu Q, Huang Y, Wang Z, Chen F, Huang D, Lu Y, et al. Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development. Int J Mol Sci. 2016;17(5):618. Doi: 10.3390/ijms17050618 Gérard N, Loiseau S, Duchamp G, Seguin F. Analysis of the variations of follicular fluid composition during follicular growth and maturation in the mare using proton nuclear magnetic resonance (1H NMR). Reprod. 2002;124(2):241-248 Gradela A, Roncoletta M, Morani C, Esper C, Franceschini P. Proteínas ligantes do insulin-like growth factor (IGFBPs) e dominância folicular em vacas Bos taurus indicus puras e cruzadas. Braz J Vet Res Anim Sci. 1998;35(5):218-220 Hill D J, Growth factors and the ircellular actions. J Reprod Fertil. 1989;85:723-734 Jans D, Hassan G, Nuclear targeting by growth factors, cytokines and their receptors: a role insignaling?. Bioessays. 1998;20:400- 411 Knight P, Glister C. Local roles of TGF-β superfamily members in the control of ovarian follicle development. Anim Reprod Sci. 2003;78:165-183 Kubelka M, Motlik J, et al. Butyrolactone I reversibly inhibits meiotic maturation of bovine oocytes, without influencing chromosome condensation activity. Biol Reprod. 2000;62:292-302 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.” Nature. 1970;227(259):680-685 Leal LS, Moya CF, Fernandes CB, Martins LR, Landim FC, Oba E. 2010. Evaluation of recovery, quality and in vitro nuclear maturation of oocytes obtained from Buffalo and Bovine Ovaries. 9no Congreso de búfalo, Argentina. Leroy J, Vanholder T, Delanghe J, Opsomer G, Van Soom A, Bols P, De Kruif A. Metabolite and ionic composition of follicular fluid from different-sized follicles and their relationship to serum concentrations in dairy cows. Anim Reprod Sci. 2004; 80(3-4):201-211 Monget P, Besnard N, Huet C, Pisselet C, Monniaux D. Insulin-like growth factor-binding proteins and ovarian folliculogenesis. Horm Res. 1996;45(3-5):211-217 Mortarino M, Vigo D, et al. Two-dimensional polyacrylamide gel electrophoresis map of bovine ovarian fluid proteins. Electrophoresis. 1999;20:866-869 Nandi S, Girish-Kumar V, Manjunatha BM, Gupta PSP. Biochem-ical composition of ovine follicular fluid in relation to follicle size. Dev.Growth Differ. 2007;49:61-66 Oberst E, Jobim M, Cimarosti H, Souza D, Salbego C, Wald V, Mattos R. Imunoidentificação de Albumina e Osteopontina no Plasma Seminal de Reprodutores Taurinos e Zebuínos. Semina: Ciências Agrárias, 2002;23(1):21-28 Otsuka F. Multiple endocrine regulation by bone morphogenetic protein system. Endocr J. 2010;57(1):3-14 Petrucci BPL, Wolf CA, Arlas TR, Santos GO, Estanislau JF, Fiala S, Mattos RC. Proteomics of mare follicular fluid during follicle development. J Equine Vet Sci. 2014;34(1):115-116 Psilopanagioti A, Papadaki H, Kranioti EF, Alexandrides TK, Varakis JN. Expression of adiponectin and adiponectin receptors in human pituitary gland and brain. Neuroendocrinology; 2009;1:38-47. doi: 10.1159/000151396 Quintana MD, Campos PEC, Herrera P, Gallego C, Padrón E. Comparación de dos métodos de recolección de ovocitos inmaduros para fertilización in vitro FIV obtenidos de hembras bubalus bubalis enviadas a matadero. Rev Salud Anim. 2012;34(1):53-56 Rahman Zia-Ur, Bukhari SA, Ahmad N, Akhtar N, Ijaz A, Yousaf MS, Haq IU. Dynamics of follicular fluid in one-humped camel (Camelus dromedarius). Reprod Domes Anim. 2008;43:664-671 Ribeiro R, Santos A, Castilho C, Giometti J, Guaberto L, Ambiel A, Giometti I. Perfil proteico do líquido folicular coletado de ovários em diferentes fases do ciclo estral de bovinos. Colloquium Agrariae. 2012;8(2):65-74 Rizos D, Burke L, Duffy P, Wade M, Mee JF, Farrel KO, et al. Comparisons between nulliparous heifers and cows as oocyte donors for embryo production in vitro. Theriogenology. 2005;63:939-949 Rodriguez F, Martinez A, Tovar S, Pinilla L, Tena-Sempere M, Dieguez C, et al. Regulation of Pituitary Cell Function by Adiponectin. Endocrinology. 2007;148(1):401-410 Shabankared HK, Kor NM, Hajarian H. The influence of the corpus luteum on metabolites composition of follicular fluid from different sized follicles and their relationship to serum concentrations in dairy cows. Anim Reprod Sci. 2013;140:109-114 Shamay A, Homans R, Fuerman Y, Levin I, Barash H, Silanikove N, et al. Expression of Albumin in Nonhepatic Tissues and its Synthesis by the Bovine Mammary Gland. J Dairy Sci. 2005;88(2):569-576 Schweigert FJ, Gericke B, Wolfram W, Kaisers U, Dudenhausen JW. Peptide and protein profiles in serum and follicular fluid of women undergoing IVF. Human Reproduction. 2006;21(11):2960-2968 Sirard MA, Richard F, et al. Controlling meiotic resumption in bovine oocytes: a review. Theriogenology. 1998;49:483-497 Solís CA, Guerra R, Sandoya G, De Armas R. Efecto de sincronización de la onda folicular y de la frecuencia de aspiración de folículos en novillas de la raza Brahman. REDVET Rev Electrón Vet. 2012;13(10):1-16 Sousa PA, Silva SJM, et al. Neurotrophin signaling in oocyte survival and developmental competence: A paradigm for cellular toti-potency. Cloning Stem Cells. 2004;6:375-385 Stojkovic M, Machado SA, et al. Mitochondrial distribution and adenosine triphosphate content of bovine oocytes before and after in vitro maturation: correlation with morphological criteria and developmental capacity after in vitro fertilization and culture. Biol Reprod. 2001;64:904-909 Sunderland SJ, Knight PG, et al. Alterations in Intrafollicular Levels of Different Molecular-Mass Forms of Inhibin During Development of Follicular-Phase and Luteal-Phase Dominant Follicles in Heifers Biol of Reprod 1996;54:453-462 Tabatabaei S, Mamoei M, Aghaei A. Dynamics of ovarian follicularfluid in cattle. Comp Clin Pathol. 2011;20:591-595 Tao J, Zhao G, Zhao X, Li F, Wu X, Hu J, Zhang Y. Proteomic analysis of the follicular fluid of Tianzhu white yak during diestrus. Int J Mol Sci. 2014;15(3):4481-4491 Ulbrich S, Frohlich T, Schulke K, Englberger E, Waldschmitt N, Arnold G, et al. Evidence for Estrogen-Dependent Uterine Serpin (SERPINA14) Expression During Estrus in the Bovine Endometrial Glandular Epithelium and Lumen. Biol Reprod. 2009;81(4):795-805 Valck SDM, De Bie J, Michiels ED, Goovaerts IG, Punjabi U, Ramos-Ibeas P, Leroy JL. The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality. Reproductive Bio Medicine Online. 2015;30(2): 203-207 Villa N, Pulgarín E, Tabares P, Angarita E, Ceballos A. Medidas corporales y concentración sérica y folicular de lípidos y glucosa en vacas Brahman fértiles y subfértiles. Pesqui Agropecu Bras. 2009;44(9):1198-1204 Waki H, Yamauchi T, Kamon J, Ito Y, Uchida S, Kita S, et al. Impaired multimerization of human adiponectin mutants associated with diabetes. Molecular structure and multimer formation of adiponectin. J Biol Chem. 2003;278(41):40352-40363 Wang W, Day B N, et al., How does polyspermy happen in mammalian oocytes Microsc Res Tech. 2003;61:335-341 Yoo S, Bolbot T, Koulova A, Sneeringer R, Humm K, Dagon Y, et al. Complement factors are secreted in human follicular fluid by granulosa cells and are possible oocyte maturation factors. J Obstet Gynaecol Res. 2013;39(2):522-527 Zachut M, Sood P, Levin Y, Moallem U. Proteomic analysis of preovulatory follicular fluid reveals differentially abundant proteins in less fertile dairy cows. J Proteomics. 2016;139:122-129
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spelling	Neira-Rivera, Elianaed4f2d0f27db1045fe1f3d8e7232c9f6Gutiérrez, Sonia L.0a0795e30b75bccb0e652ea471f5b08c300Castillo-Barón, Lidy V.82d952181b242123f49440ae979913bf300Velásquez-Penagos, José G.5cd7b260c0ce61fc07a0a341c50d01e3500Góngora-Orjuela, Agustín19950225dead911a31cee2c4909e496fCardozo-Cerquera, Jaime A.c2cfb8def8db87e6f12b8faf68108ca13002020-05-11 00:00:002022-06-13T17:42:39Z2020-05-11 00:00:002022-06-13T17:42:39Z2020-05-110121-3709https://repositorio.unillanos.edu.co/handle/001/274910.22579/20112629.5982011-2629https://doi.org/10.22579/20112629.598El objetivo de este estudio fue determinar la expresión de proteínas del fluido folicular (FF) y su relación con la calidad del oocito. Se evaluaron 52 ovarios de planta de faenado de vacas Cebú comercial, mediante la técnica de disección y aspiración folicular se obtuvo FF y oocitos. Las evaluaciones realizadas fueron: calidad del oocito por aspecto citoplasmático y células del cúmulos y perfil de proteínas del FF mediante SDS-PAGE. Se realizó el análisis descriptivo, a través del procedimiento MEANS, análisis de varianza (PROC. ANOVA) y para las diferencias estadísticas significativas se usó la prueba de comparación de Bonferroni con un nivel de significancia del 5%, mediante el paquete estadístico SAS®. El 52% de los oocitos se categorizaron con calidad I-II. El análisis unidimensional de las proteínas del FF evidenció la presencia de 25 bandas de proteína entre 9 y 240 kDa. En folículos &lt;3 mm se expresaron 23 bandas, en folículos de 3 y 6 mm 19 bandas y en folículos &gt;6mm 20 bandas. Las bandas de peso molecular (PM) de 26kDa, 57kDa y 68kDa representan la mayor concentración en el FF; 4 bandas de PM 14 KDa, 34 KDa, 76 y 79 KDa, solo en folículos de &lt;3mm, 2 bandas de PM 9 y 91 KDa solo en folículos de &gt;3 mm. La banda de 32 KDa no se observó en folículos &gt; de 6mm. Las bandas de mayor frecuencia de presentación fueron las de 26, 40, 42, 57, 68, 240 KDa. Las bandas de proteína que se asociaron con la calidad del oocito en forma significativa (p&lt;0,05) fueron las de PM 24, 57, 68 y 164 KDa para FF de folículos &lt;3mm y las bandas de PM 13, 26 y 38 kDa entre 3 y 6mm, y la de 26 kDa a folículos &gt; de 6mm. Los resultados nos indican asociaciones de la calidad del oocito con algunas bandas de proteína.This study was aimed at determining follicular fluid (FF) protein expression and its relationship with oocyte quality. FF and oocytes were obtained by dissection and follicular aspiration of the ovaries from fifty-two commercial Zebu from a slaughterhouse. Oocyte quality was measured by cytoplasmic aspect and cumulus cells and FF protein profile by SDS-PAGE. The SAS statistical package’s PROC MEANS and analysis of variance (ANOVA) were used for descriptive analysis and the Bonferroni comparison test for assessing significant statistical differences (5% significance level); 52% of the oocytes were categorised as having I-II quality. One-dimensional SDS-PAGE analysis of FF proteins revealed 25 protein bands having 9 kDa to 240 kDa molecular weight (MW); 23 bands were expressed in &lt;3 mm follicles, 19 bands in 3 and 6 mm follicles and 20 bands in &gt;6 mm follicles. The 26kDa, 57kDa and 68kDa bands’ MW represented the highest FF concentration whereas only four bands (14 kDa, 34 kDa, 76 and 79 kDa MW) were found in &lt;3mm follicles and only 2 bands (9kDa and 91 kDa MW) in &gt;3 mm follicles. The 32 kDa band was not observed in &gt;6mm follicles. The 26, 40, 42, 57, 68 and 240 KDa bands occurred with the greatest frequency. The protein bands which were significantly associated with oocyte quality (p&lt;0.05) were 24, 57, 68 and 164 kDa MW for &lt;3mm follicles, 13, 26 and 38 kDa MW bands for 3 and 6mm follicles and 26 kDa for &gt;6mm follicles. The results indicated oocyte quality association with some protein bands.application/pdfspaUniversidad de los LlanosOrinoquia - 2020https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/598Mineralizationpetroleum spillssoil pollutionphytoremediationbioremediationmineralizaciónderrames de petróleocontaminación de suelosfitorremediaciónbiorremediaciónExpresión proteica del fluido folicular asociado a la calidad del oocito de vacas CebúFollicular fluid (FF) protein expression associated with oocyte quality in Zebu cattleArtículo de revistaJournal Articleinfo:eu-repo/semantics/articleSección Ciencias agrariasSección Agricultural sciencesinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Texthttp://purl.org/coar/version/c_970fb48d4fbd8a85Alavi-Shoushtari S, Asri-Rezai S, Abshenas J. A study of the uterine protein variations during the estrus cycle in the cow: A comparison with the serum proteins. Anim Reprod Sci, 2006;96(1-2):10-20Aller JF, Callejas SS, Alberio RH. Biochemical and steroid concentrations in follicular fluid and blood plasma in different follicular waves of the estrous cycle from normal and superovulated beef cows. Anim Reprod Sci, 2013;142:113-120Ambekar A, Nirujogi R, Srikanth S, Chavan S, Kelkar D, Hinduja I, et al. Proteomic analysis of human follicular fluid: A new perspective towards understanding folliculogenesis. J Proteomics. 2013;87:68-77Andersen M, Kroll J, Byskov A, Faber M. Protein composition in the fluid of individual bovine follicles. J Reprod Fertil. 1976;48(1):109-118Angelucci S, Ciavardelli D, Di Giuseppe F, Eleuterio E, Sulpizio M, Tiboni GM, et al. Proteome analysis of human follicular fluid. Biochim Biophys Acta - Proteins Proteomics. 2006;1764(11):1775-1785Armstrong D, Hogg C, Campbell B, Webb R. 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J Proteomics. 2016;139:122-129https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/598/pdfNúm. 1 , Año 20204113224OrinoquiaPublicationOREORE.xmltext/xml2562https://dspace7-unillanos.metacatalogo.org/bitstreams/6a543321-7469-4a41-bd54-6a400e2d68c1/download8d8e899ce57e6864c4f8b9ed45fd6133MD51001/2749oai:dspace7-unillanos.metacatalogo.org:001/27492024-04-17 16:40:44.077https://creativecommons.org/licenses/by/4.0/Orinoquia - 2020metadata.onlyhttps://dspace7-unillanos.metacatalogo.orgRepositorio Universidad de Los Llanosrepositorio@unillanos.edu.co

Expresión proteica del fluido folicular asociado a la calidad del oocito de vacas Cebú

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