Involvement of sialic acid in bovine sperm-zona pellucida interaction

ABSTRAC: perm-egg interaction is a complex process mediated by receptors and ligands present  in both gametes, spermatozoon and  oocyte. The important role played by the carbohydrates in the gamete interaction  is well documented. However, the identification of the molecules involved in this process...

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Autores:
Velásquez, J. G
Marcos, J
Gardón, JC
Jiménez-Movilla, M
Ballesta, J
Coy, P
Avilés, M
Tipo de recurso:
Article of journal
Fecha de publicación:
2004
Institución:
Universidad de los Llanos
Repositorio:
Repositorio Digital Universidad de los LLanos
Idioma:
spa
OAI Identifier:
oai:repositorio.unillanos.edu.co:001/3704
Acceso en línea:
https://repositorio.unillanos.edu.co/handle/001/3704
https://doi.org/10.22579/20112629.181
Palabra clave:
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openAccess
License
Orinoquia - 2014
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spelling Velásquez, J. GMarcos, JGardón, JCJiménez-Movilla, MBallesta, JCoy, PAvilés, M2004-01-01T00:00:00Z2024-07-25T18:13:35Z2004-01-01T00:00:00Z2024-07-25T18:13:35Z2004-01-010121-3709https://repositorio.unillanos.edu.co/handle/001/370410.22579/20112629.1812011-2629https://doi.org/10.22579/20112629.181ABSTRAC: perm-egg interaction is a complex process mediated by receptors and ligands present  in both gametes, spermatozoon and  oocyte. The important role played by the carbohydrates in the gamete interaction  is well documented. However, the identification of the molecules involved in this process remains unsolved. A carbohydrate modification in the sialic acid contents of the bovine zona pellucida (ZP) post fertilization has been previously reported. However, no evidence exists on the implication of this carbohydrate in the sperm-ZP  interaction.  In this study, we investigate the role played by the sialic acid residues in the sperm-ZP  binding by means of different sperm binding competition and IVF assays with different sialic acid specific lectins, neuraminidase enzyme and antibodies.application/mswordapplication/pdftext/htmlspaUniversidad de los LlanosOrinoquia - 2014https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/181Involvement of sialic acid in bovine sperm-zona pellucida interactionInvolvement of sialic acid in bovine sperm-zona pellucida interactionArtículo de revistainfo:eu-repo/semantics/articleJournal articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Texthttp://purl.org/coar/version/c_970fb48d4fbd8a85https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/181/331https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/181/332https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/181/642241248OrinoquiaPublicationOREORE.xmltext/xml2766https://repositorio.unillanos.edu.co/bitstreams/e8a07fd8-9d0d-400a-859c-78c5ad4cd7f9/download92219ee93c67aaf3bc1776d12e478785MD51001/3704oai:repositorio.unillanos.edu.co:001/37042024-07-25 13:13:35.19https://creativecommons.org/licenses/by-nc-sa/4.0/Orinoquia - 2014metadata.onlyhttps://repositorio.unillanos.edu.coRepositorio Universidad de Los Llanosrepositorio@unillanos.edu.co
dc.title.spa.fl_str_mv Involvement of sialic acid in bovine sperm-zona pellucida interaction
dc.title.translated.eng.fl_str_mv Involvement of sialic acid in bovine sperm-zona pellucida interaction
title Involvement of sialic acid in bovine sperm-zona pellucida interaction
spellingShingle Involvement of sialic acid in bovine sperm-zona pellucida interaction
title_short Involvement of sialic acid in bovine sperm-zona pellucida interaction
title_full Involvement of sialic acid in bovine sperm-zona pellucida interaction
title_fullStr Involvement of sialic acid in bovine sperm-zona pellucida interaction
title_full_unstemmed Involvement of sialic acid in bovine sperm-zona pellucida interaction
title_sort Involvement of sialic acid in bovine sperm-zona pellucida interaction
dc.creator.fl_str_mv Velásquez, J. G
Marcos, J
Gardón, JC
Jiménez-Movilla, M
Ballesta, J
Coy, P
Avilés, M
dc.contributor.author.spa.fl_str_mv Velásquez, J. G
Marcos, J
Gardón, JC
Jiménez-Movilla, M
Ballesta, J
Coy, P
Avilés, M
description ABSTRAC: perm-egg interaction is a complex process mediated by receptors and ligands present  in both gametes, spermatozoon and  oocyte. The important role played by the carbohydrates in the gamete interaction  is well documented. However, the identification of the molecules involved in this process remains unsolved. A carbohydrate modification in the sialic acid contents of the bovine zona pellucida (ZP) post fertilization has been previously reported. However, no evidence exists on the implication of this carbohydrate in the sperm-ZP  interaction.  In this study, we investigate the role played by the sialic acid residues in the sperm-ZP  binding by means of different sperm binding competition and IVF assays with different sialic acid specific lectins, neuraminidase enzyme and antibodies.
publishDate 2004
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2024-07-25T18:13:35Z
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dc.identifier.eissn.none.fl_str_mv 2011-2629
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dc.relation.ispartofjournal.spa.fl_str_mv Orinoquia
dc.rights.spa.fl_str_mv Orinoquia - 2014
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