Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda

Edwardsiella tarda es una bacteria Gram-negativa responsable de edwardsiellosis, una enfermedad de peces y mamíferos incluido el humano, la cual se caracteriza por la formación de múltiples abscesos. Distintas cepas de E. tarda poseen un número de genes asociados con virulencia, resistencia a múltip...

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Autores:: Verjan-García, Noel
Carlos, A.
Ikuo Hirono, Iregui

Tipo de recurso:: Article of journal

Fecha de publicación:: 2015

Institución:: Universidad de los Llanos

Repositorio:: Repositorio Digital Universidad de los LLanos

Idioma:: spa

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dc.title.spa.fl_str_mv	Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda
dc.title.translated.eng.fl_str_mv	Novel type 4 secretion system (T4SS)-related genes of Edwardsiella tarda
title	Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda
spellingShingle	Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda Pathogenesis T4SS genes Virulence Patogenesis Sistema secretor tipo IV Virulencia Pathogenesis T4SS genes Virulence
title_short	Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda
title_full	Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda
title_fullStr	Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda
title_full_unstemmed	Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda
title_sort	Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda
dc.creator.fl_str_mv	Verjan-García, Noel Carlos, A. Ikuo Hirono, Iregui
dc.contributor.author.spa.fl_str_mv	Verjan-García, Noel Carlos, A. Ikuo Hirono, Iregui
dc.subject.spa.fl_str_mv	Pathogenesis T4SS genes Virulence Patogenesis Sistema secretor tipo IV Virulencia
topic	Pathogenesis T4SS genes Virulence Patogenesis Sistema secretor tipo IV Virulencia Pathogenesis T4SS genes Virulence
dc.subject.eng.fl_str_mv	Pathogenesis T4SS genes Virulence
description	Edwardsiella tarda es una bacteria Gram-negativa responsable de edwardsiellosis, una enfermedad de peces y mamíferos incluido el humano, la cual se caracteriza por la formación de múltiples abscesos. Distintas cepas de E. tarda poseen un número de genes asociados con virulencia, resistencia a múltiples antibióticos y sistemas secretores de toxinas que explican en cierto grado su capacidad de sobrevivir dentro de células fagocíticas y de infectar a diversos hospederos. En estudios previos, nuestro grupo secuencio parcialmente el genoma de una cepa virulenta de E. tarda (ETSJ54) aislada de lenguado japonés (Paralichthys olivaceus) con edwardsiellosis y reportó un número de genes asociados a su virulencia. En este estudio se ha re-analizado los datos de secuenciación y en este proceso se identificaron varios genes que codifican para la estructura de superficie Pili y el sistema secretor tipo IV, la mayoría de los cuales estuvieron rodeados por genes codificadores de transposasas y otros genes de origen plasmídico. La secuencia de nucleótidos de dichos genes no tuvieron identidad con la de los genes previamente reportados en E. tarda EIB202, una cepa virulenta aislada de turbot (Scophthalmus maximus) en China. Los resultados sugieren diferencias en el contenido genético de cepas de E. tarda de distinto origen geográfico y la necesidad de desarrollar nuevos proyectos de secuenciamiento de genomas de E. tarda.
publishDate	2015
dc.date.accessioned.none.fl_str_mv	2015-07-01T00:00:00Z 2024-07-25T18:14:10Z
dc.date.available.none.fl_str_mv	2015-07-01T00:00:00Z 2024-07-25T18:14:10Z
dc.date.issued.none.fl_str_mv	2015-07-01
dc.type.spa.fl_str_mv	Artículo de revista
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dc.relation.references.spa.fl_str_mv	Miyazaki T, Kaige N. Comparative histopathology of edwardsiellosis in fishes. Fish Pathol. 1985;20:219-227. Iregui CA, Guarin M, Tibata VM, Ferguson HW. Novel brain lesions caused by Edwardsiella tarda in a red tilapia (Oreochromis spp.). J Vet Diagn Invest. 2012;24:446-449. Verjan N, Iregui CA, Hirono I. Edwardsiellosis, common and novel manifestations of the disease: A review. Revista Colombiana de Ciencia Animal, RCCA. 2012;5:73-82. Wang IK, Kuo HL, Chen YM, Lin CL, Chang HY, Chuang FR, et al. Extraintestinal manifestations of Edwardsiella tarda infection. Int J Clin Pract. 2005;59:917-921. Spencer JD, Hastings MC, Rye AK, English BK, Ault BH. Gastroenteritis caused by Edwardsiella tarda in a pediatric renal transplant recipient. Pediatr Transplant. 2008;12:238-241 Wang Q, Yang M, Xiao J, Wu H, Wang X, Lv Y, et al. Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS One. 2009;4:e7646. Hirono I, Tange N, Aoki T. Iron-regulated haemolysin gene from Edwardsiella tarda. Mol Microbiol. 1997;24:851-856. Mathew JA, Tan YP, Srinivasa Rao PS, Lim TM, Leung KY. Edwardsiella tarda mutants defective in siderophore production, motility, serum resistance and catalase activity. Microbiology. 2001;147:449-457. Rao PS, Yamada Y, Tan YP, Leung KY. Use of proteomics to identify novel virulence determinants that are required for Edwardsiella tarda pathogenesis. Mol Microbiol. 2004;53:573-586. Zheng J, Tung SL, Leung KY. Regulation of a type III and a putative secretion system in Edwardsiella tarda by EsrC is under the control of a two-component system, EsrA-EsrB. Infect Immun. 2005;73:4127-4137. Okuda J, Arikawa Y, Takeuchi Y, Mahmoud MM, Suzaki E, Kataoka K, et al. Intracellular replication of Edwardsiella tarda in murine macrophage is dependent on the type III secretion system and induces an up-regulation of anti-apoptotic NF-kappaB target genes protecting the macrophage from staurosporine-induced apoptosis. Microb Pathog. 2006;41:226-240. Ishibe K, Osatomi K, Hara K, Kanai K, Yamaguchi K, Oda T. Comparison of the responses of peritoneal macrophages from Japanese flounder (Paralichthys olivaceus) against high virulent and low virulent strains of Edwardsiella tarda. Fish Shellfish Immunol. 2008;24:243-251. Cheng S, Zhang M, Sun L. The iron-cofactored superoxide dismutase of Edwardsiella tarda inhibits macrophage-mediated innate immune response. Fish Shellfish Immunol. 2010;29:972-978. Dang W, Hu YH, Sun L. HtpG is involved in the pathogenesis of Edwardsiella tarda. Vet Microbiol. 2011;152:394-400. Backert S, Meyer TF. Type IV secretion systems and their effectors in bacterial pathogenesis. Curr Opin Microbiol. 2006;9:207-217. Verjan N, Hirono I, Aoki T. Genetic loci of major antigenic protein genes of Edwardsiella tarda. Appl Environ Microbiol. 2005;71:5654-5658. Verjan N. Virulence-related and antigenic protein coding genes of Edwardsiella tarda. PhD thesis, Tokyo University of Marine Science and Technology, Tokyo, Japan. 2005b. Verjan N, Iregui C, Hirono I. A random genome analysis of Edwardsiella tarda ETSJ54: annotation of putative virulence-related genes. Orinoquia. 2013;17(1):69-83. Hou JH, Zhang WW, Sun L. Immunoprotective analysis of two Edwardsiella tarda antigens. J Gen Appl Microbiol. 2009;55:57-61. Sambrook J, Russell DW. 2001. Molecular cloning. A Laboratory Manual. Third edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Ausubel FH, Brent R, Kingston E, Moore DD, Seidman JG, Smith JA, et al. 1994. Current protocols in Molecular Biology. John Wiley and Son. Zhang Z, Schwartz S, Wagner L,Miller W. A greedy algorithm for aligning DNA sequences. J Comput Biol. 2000;7:203-214. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, and Kumar S. Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol. 2011;28:2731-2739. Tekedar HC, Karsi A, Williams ML, Vamenta S, Banes MM, Duke M, et al. 2013. Genome sequence of the fish pathogen Edwarsiella tarda C07-087. Published only in database. Amano A. Bacterial adhesins to host components in periodontitis. Periodontol 2000 2010;52:12-37. Friedlander RS, Vlamakis H, Kim P, Khan M, Kolter R, Aizenberg J. Bacterial flagella explore microscale hummocks and hollows to increase adhesion. Proc Natl Acad Sci U S A. 2013;110:56245629. Confer AW, Ayalew S. The OmpA family of proteins: roles in bacterial pathogenesis and immunity. Vet Microbiol. 2013;163:207-222. Jin RP, Hu YH, Sun BG, Zhang XH, Sun L. Edwardsiella tarda sialidase: pathogenicity involvement and vaccine potential. Fish Shellfish Immunol. 2012;33:514-521. Yu JE, Cho MY, Kim JW, Kang HY. Large antibiotic-resistance plasmid of Edwardsiella tarda contributes to virulence in fish. Microb Pathog. 2012;52:259-266. Bruggemann H, Cazalet C, Buchrieser C. Adaptation of Legionella pneumophila to the host environment: role of protein secretion, effectors and eukaryotic-like proteins. Curr Opin Microbiol. 2006;9:86-94. Llosa M, Schroder G, Dehio C. New perspectives into bacterial DNA transfer to human cells. Trends Microbiol. 2012;20:355-359. Juhas M, Crook DW, Hood DW. Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence. Cell Microbiol. 2008;10:2377-2386. Alvarez-Martinez CE, Christie PJ. Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 2009;73:775-808. Souza RC, del Rosario Quispe Saji G, Costa MO, Netto DS, Lima NC, Klein CC, et al. AtlasT4SS: a curated database for type IV secretion systems. BMC Microbiol. 2012;12:172. Lawley TD, Klimke WA, Gubbins MJ, Frost LS. F factor conjugation is a true type IV secretion system. FEMS Microbiol Lett. 2003;224:1-15. Frost LS, Ippen-Ihler K, Skurray RA. Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol Rev. 1994;58:162-210. Backert S, Kwok T, Konig W. Conjugative plasmid DNA transfer in Helicobacter pylori mediated by chromosomally encoded relaxase and TraG-like proteins. Microbiology. 2005;151:34933503.
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dc.relation.ispartofjournal.spa.fl_str_mv	Orinoquia
dc.rights.spa.fl_str_mv	Orinoquia - 2016
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spelling	Verjan-García, NoelCarlos, A.Ikuo Hirono, Iregui2015-07-01T00:00:00Z2024-07-25T18:14:10Z2015-07-01T00:00:00Z2024-07-25T18:14:10Z2015-07-010121-3709https://repositorio.unillanos.edu.co/handle/001/382510.22579/20112629.3182011-2629https://doi.org/10.22579/20112629.318Edwardsiella tarda es una bacteria Gram-negativa responsable de edwardsiellosis, una enfermedad de peces y mamíferos incluido el humano, la cual se caracteriza por la formación de múltiples abscesos. Distintas cepas de E. tarda poseen un número de genes asociados con virulencia, resistencia a múltiples antibióticos y sistemas secretores de toxinas que explican en cierto grado su capacidad de sobrevivir dentro de células fagocíticas y de infectar a diversos hospederos. En estudios previos, nuestro grupo secuencio parcialmente el genoma de una cepa virulenta de E. tarda (ETSJ54) aislada de lenguado japonés (Paralichthys olivaceus) con edwardsiellosis y reportó un número de genes asociados a su virulencia. En este estudio se ha re-analizado los datos de secuenciación y en este proceso se identificaron varios genes que codifican para la estructura de superficie Pili y el sistema secretor tipo IV, la mayoría de los cuales estuvieron rodeados por genes codificadores de transposasas y otros genes de origen plasmídico. La secuencia de nucleótidos de dichos genes no tuvieron identidad con la de los genes previamente reportados en E. tarda EIB202, una cepa virulenta aislada de turbot (Scophthalmus maximus) en China. Los resultados sugieren diferencias en el contenido genético de cepas de E. tarda de distinto origen geográfico y la necesidad de desarrollar nuevos proyectos de secuenciamiento de genomas de E. tarda.Edwardsiella tarda is a Gram-negative bacterium that causes edwardsiellosis, a disease of fish and mammals including humans and characterized by multiple abscesses. Different strains of E. tarda possess a number of virulence, antibioticresistance, and toxin secretion system-related genes that explain in some extent its capacity to survive within phagocytic cells and to infect a variety of hosts. Previously we introduced a virulent E. tardastrain (ETSJ54) isolated from Japanese flounder (Paralichthys olivaceus) with edwardsiellosis and reported a number of virulence-related genes. In this study we have re-analyzed the sequencing data of ETSJ54 and identified novel type IV secretion system-related genes, most of them were flanked by transposase and plasmid encoding genes. Interestingly, their nucleotide sequence had no identity to those of the genes published in the E. tarda EIB202 genome, a virulent strain isolated from turbot (Scophthalmus maximus) in China. The results suggest differences in gene content between geographically distinct E. tarda strains that may encourage additional E. tarda genome sequencing projects.Keywords: Pathogenesis; T4SS genes; Virulence.application/pdftext/htmlspaUniversidad de los LlanosOrinoquia - 2016https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/318PathogenesisT4SS genesVirulencePatogenesisSistema secretor tipo IVVirulenciaPathogenesisT4SS genesVirulenceNuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tardaNovel type 4 secretion system (T4SS)-related genes of Edwardsiella tardaArtículo de revistainfo:eu-repo/semantics/articleJournal articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Texthttp://purl.org/coar/version/c_970fb48d4fbd8a85Miyazaki T, Kaige N. Comparative histopathology of edwardsiellosis in fishes. Fish Pathol. 1985;20:219-227.Iregui CA, Guarin M, Tibata VM, Ferguson HW. Novel brain lesions caused by Edwardsiella tarda in a red tilapia (Oreochromis spp.). J Vet Diagn Invest. 2012;24:446-449.Verjan N, Iregui CA, Hirono I. Edwardsiellosis, common and novel manifestations of the disease: A review. Revista Colombiana de Ciencia Animal, RCCA. 2012;5:73-82.Wang IK, Kuo HL, Chen YM, Lin CL, Chang HY, Chuang FR, et al. Extraintestinal manifestations of Edwardsiella tarda infection. Int J Clin Pract. 2005;59:917-921.Spencer JD, Hastings MC, Rye AK, English BK, Ault BH. Gastroenteritis caused by Edwardsiella tarda in a pediatric renal transplant recipient. Pediatr Transplant. 2008;12:238-241Wang Q, Yang M, Xiao J, Wu H, Wang X, Lv Y, et al. Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS One. 2009;4:e7646.Hirono I, Tange N, Aoki T. Iron-regulated haemolysin gene from Edwardsiella tarda. Mol Microbiol. 1997;24:851-856.Mathew JA, Tan YP, Srinivasa Rao PS, Lim TM, Leung KY. Edwardsiella tarda mutants defective in siderophore production, motility, serum resistance and catalase activity. Microbiology. 2001;147:449-457.Rao PS, Yamada Y, Tan YP, Leung KY. Use of proteomics to identify novel virulence determinants that are required for Edwardsiella tarda pathogenesis. Mol Microbiol. 2004;53:573-586.Zheng J, Tung SL, Leung KY. Regulation of a type III and a putative secretion system in Edwardsiella tarda by EsrC is under the control of a two-component system, EsrA-EsrB. Infect Immun. 2005;73:4127-4137.Okuda J, Arikawa Y, Takeuchi Y, Mahmoud MM, Suzaki E, Kataoka K, et al. Intracellular replication of Edwardsiella tarda in murine macrophage is dependent on the type III secretion system and induces an up-regulation of anti-apoptotic NF-kappaB target genes protecting the macrophage from staurosporine-induced apoptosis. Microb Pathog. 2006;41:226-240.Ishibe K, Osatomi K, Hara K, Kanai K, Yamaguchi K, Oda T. Comparison of the responses of peritoneal macrophages from Japanese flounder (Paralichthys olivaceus) against high virulent and low virulent strains of Edwardsiella tarda. Fish Shellfish Immunol. 2008;24:243-251.Cheng S, Zhang M, Sun L. The iron-cofactored superoxide dismutase of Edwardsiella tarda inhibits macrophage-mediated innate immune response. Fish Shellfish Immunol. 2010;29:972-978.Dang W, Hu YH, Sun L. HtpG is involved in the pathogenesis of Edwardsiella tarda. Vet Microbiol. 2011;152:394-400.Backert S, Meyer TF. Type IV secretion systems and their effectors in bacterial pathogenesis. Curr Opin Microbiol. 2006;9:207-217.Verjan N, Hirono I, Aoki T. Genetic loci of major antigenic protein genes of Edwardsiella tarda. Appl Environ Microbiol. 2005;71:5654-5658.Verjan N. Virulence-related and antigenic protein coding genes of Edwardsiella tarda. PhD thesis, Tokyo University of Marine Science and Technology, Tokyo, Japan. 2005b. Verjan N, Iregui C, Hirono I. A random genome analysis of Edwardsiella tarda ETSJ54: annotation of putative virulence-related genes. Orinoquia. 2013;17(1):69-83.Hou JH, Zhang WW, Sun L. Immunoprotective analysis of two Edwardsiella tarda antigens. J Gen Appl Microbiol. 2009;55:57-61.Sambrook J, Russell DW. 2001. Molecular cloning. A Laboratory Manual. Third edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Ausubel FH, Brent R, Kingston E, Moore DD, Seidman JG, Smith JA, et al. 1994. Current protocols in Molecular Biology. John Wiley and Son. Zhang Z, Schwartz S, Wagner L,Miller W. A greedy algorithm for aligning DNA sequences. J Comput Biol. 2000;7:203-214.Tamura K, Peterson D, Peterson N, Stecher G, Nei M, and Kumar S. Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol. 2011;28:2731-2739.Tekedar HC, Karsi A, Williams ML, Vamenta S, Banes MM, Duke M, et al. 2013. Genome sequence of the fish pathogen Edwarsiella tarda C07-087. Published only in database. Amano A. Bacterial adhesins to host components in periodontitis. Periodontol 2000 2010;52:12-37.Friedlander RS, Vlamakis H, Kim P, Khan M, Kolter R, Aizenberg J. Bacterial flagella explore microscale hummocks and hollows to increase adhesion. Proc Natl Acad Sci U S A. 2013;110:56245629.Confer AW, Ayalew S. The OmpA family of proteins: roles in bacterial pathogenesis and immunity. Vet Microbiol. 2013;163:207-222.Jin RP, Hu YH, Sun BG, Zhang XH, Sun L. Edwardsiella tarda sialidase: pathogenicity involvement and vaccine potential. Fish Shellfish Immunol. 2012;33:514-521.Yu JE, Cho MY, Kim JW, Kang HY. Large antibiotic-resistance plasmid of Edwardsiella tarda contributes to virulence in fish. Microb Pathog. 2012;52:259-266.Bruggemann H, Cazalet C, Buchrieser C. Adaptation of Legionella pneumophila to the host environment: role of protein secretion, effectors and eukaryotic-like proteins. Curr Opin Microbiol. 2006;9:86-94.Llosa M, Schroder G, Dehio C. New perspectives into bacterial DNA transfer to human cells. Trends Microbiol. 2012;20:355-359.Juhas M, Crook DW, Hood DW. Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence. Cell Microbiol. 2008;10:2377-2386.Alvarez-Martinez CE, Christie PJ. Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 2009;73:775-808.Souza RC, del Rosario Quispe Saji G, Costa MO, Netto DS, Lima NC, Klein CC, et al. AtlasT4SS: a curated database for type IV secretion systems. BMC Microbiol. 2012;12:172.Lawley TD, Klimke WA, Gubbins MJ, Frost LS. F factor conjugation is a true type IV secretion system. FEMS Microbiol Lett. 2003;224:1-15.Frost LS, Ippen-Ihler K, Skurray RA. Analysis of the sequence and gene products of the transfer region of the F sex factor. Microbiol Rev. 1994;58:162-210.Backert S, Kwok T, Konig W. Conjugative plasmid DNA transfer in Helicobacter pylori mediated by chromosomally encoded relaxase and TraG-like proteins. Microbiology. 2005;151:34933503.https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/318/884https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/318/html_10194218619OrinoquiaPublicationOREORE.xmltext/xml2615https://repositorio.unillanos.edu.co/bitstreams/b7e09ceb-15d1-4a07-a644-87c9ebe115fb/download96c0afde3a76bcea7ef33cf69d3cb5efMD51001/3825oai:repositorio.unillanos.edu.co:001/38252024-07-25 13:14:10.658https://creativecommons.org/licenses/by-nc-sa/4.0/Orinoquia - 2016metadata.onlyhttps://repositorio.unillanos.edu.coRepositorio Universidad de Los Llanosrepositorio@unillanos.edu.co

Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda

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