Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando

El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en hum...

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Autores:
Vejarano-Orjuela, Álvaro
Hernández-Vásquez, Aureliano
Rondón-Barragán, Iang S.
Suárez-Mesa, Rafael A.
Tipo de recurso:
Article of journal
Fecha de publicación:
2020
Institución:
Universidad de los Llanos
Repositorio:
Repositorio Digital Universidad de los LLanos
Idioma:
spa
OAI Identifier:
oai:repositorio.unillanos.edu.co:001/2743
Acceso en línea:
https://repositorio.unillanos.edu.co/handle/001/2743
https://doi.org/10.22579/20112629.590
Palabra clave:
Inbreeding
heterosis
crossing
additive
dominant
combining ability
Endogamia
heterosis
cruzamiento
aditivo
dominante
habilidad combinatoria
Rights
openAccess
License
Orinoquia - 2020
id Unillanos2_252364c99253f246758316637a7e447c
oai_identifier_str oai:repositorio.unillanos.edu.co:001/2743
network_acronym_str Unillanos2
network_name_str Repositorio Digital Universidad de los LLanos
repository_id_str
dc.title.spa.fl_str_mv Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
dc.title.translated.eng.fl_str_mv Luteinizing hormone choriogonadotropin receptor (LHGCR) gene polymorphism in ovarian follicles from cycling Romosinuano and Brahman cows
title Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
spellingShingle Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
Inbreeding
heterosis
crossing
additive
dominant
combining ability
Endogamia
heterosis
cruzamiento
aditivo
dominante
habilidad combinatoria
title_short Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
title_full Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
title_fullStr Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
title_full_unstemmed Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
title_sort Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
dc.creator.fl_str_mv Vejarano-Orjuela, Álvaro
Hernández-Vásquez, Aureliano
Rondón-Barragán, Iang S.
Suárez-Mesa, Rafael A.
dc.contributor.author.spa.fl_str_mv Vejarano-Orjuela, Álvaro
Hernández-Vásquez, Aureliano
Rondón-Barragán, Iang S.
Suárez-Mesa, Rafael A.
dc.subject.eng.fl_str_mv Inbreeding
heterosis
crossing
additive
dominant
combining ability
topic Inbreeding
heterosis
crossing
additive
dominant
combining ability
Endogamia
heterosis
cruzamiento
aditivo
dominante
habilidad combinatoria
dc.subject.spa.fl_str_mv Endogamia
heterosis
cruzamiento
aditivo
dominante
habilidad combinatoria
description El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ovario que contenía un folículo preovulatorio, se retiró la pared folicular, se extrajo el RNA total y se sintetizó, amplificó, secuenció y comparó el ADNc contra secuencias ya reportadas, presentando una alta similitud. Se concluye que en estas razas la ausencia de variantes en la expresión del gen, estaría asociada a buen desempeño reproductivo.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-05-11 00:00:00
2022-06-13T17:42:37Z
dc.date.available.none.fl_str_mv 2020-05-11 00:00:00
2022-06-13T17:42:37Z
dc.date.issued.none.fl_str_mv 2020-05-11
dc.type.spa.fl_str_mv Artículo de revista
dc.type.eng.fl_str_mv Journal Article
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.local.spa.fl_str_mv Sección Ciencias agrarias
dc.type.local.eng.fl_str_mv Sección Agricultural sciences
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dc.identifier.doi.none.fl_str_mv 10.22579/20112629.590
dc.identifier.eissn.none.fl_str_mv 2011-2629
dc.identifier.url.none.fl_str_mv https://doi.org/10.22579/20112629.590
identifier_str_mv 0121-3709
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url https://repositorio.unillanos.edu.co/handle/001/2743
https://doi.org/10.22579/20112629.590
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.references.spa.fl_str_mv Cadagan D, Towlson C. Mechanisms of Luteinising Hormone Regulation in Female Steroidogenesis. Am J Med, 2017;5:65-68.
Casarini L, Pignatti E, Simoni M. Effects of polymorphisms in gonadotropin and gonadotropin receptor genes on reproductive function. Rev Endocr Metab Dis, 2011;12:303.
Clelland E, Peng C. Endocrine/paracrine control of zebrafish ovarian development. Mol Cell Endocrinol, 2009;312:42-52.
Delemi A. Identificaion of the expression level to LH-r Gene in dominant and cystic ovarian follicles cells of the cow.
Al-Delemi DHJ, Al-Gewary AKA, Ali Jeddoa Z. Identification of the expression level to LH-r gene in dominant and cystic ovarian follicles cells of the cows. J Dairy Vet Anim Res, 2014;1(3):80-86. DOI: 10.15406/jdvar.2014.01.00017
Dickinson RE, Stewart AJ, Myers M, Millar RP Duncan WC. Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells: implications for luteolysis. Endocrinology, 2009;150:2873-2881.
Gay V, Midgley Jr A, Niswender G. (1970). Patterns of gonadotrophin secretion associated with ovulation. Federation proceedings, p. 1880.
Ginther O. Selection of the dominant follicle in cattle and horses. Anim Reprod Sci, 2000;60:61-79.
Hastings N, Donn S, Derecka K, Flint A, Woolliams J. Polymorphisms within the coding region of the bovine luteinizing hormone receptor gene and their association with fertility traits. Anim Genet, 2006;37:583-585.
Holdridge LR. 1967. Life zone ecology. Life zone ecology.
Johnson A, Bridgham J, Wagner B. Characterization of a chicken luteinizing hormone receptor (cLH-R) complementary deoxyribonucleic acid, and expression of cLH-R messenger ribonucleic acid in the ovary. Biol Reprod, 1996;55: 304-309.
Kawate N. Studies on the regulation of expression of luteinizing hormone receptor in the ovary and the mechanism of follicular cyst formation in ruminants. J Reprod Develop, 2004;50:1-8.
Larsson B. Determination of Ovulation by Ultrasound Examination and its Relation to the LH‐Peak in Heifers. J Vet Med A, 1987;34:749-754.
Liu KC, Lin SW, Ge W. Differential regulation of gonadotropin receptors (fshr and lhcgr) by estradiol in the zebrafish ovary involves nuclear estrogen receptors that are likely located on the plasma membrane. Endocrinology, 2011;152:4418-4430.
Ma T, Xiong Q, Yuan B, Jiang H, Gao Y, Xu J, Liu S, et al. Luteinizing hormone receptor splicing variants in bovine Leydig cells. Genet Mol Res, 2012;11:1721-1730.
Mamluk R, Wolfenson D, Meidan R. LH receptor mRNA and cytochrome P450 side-chain cleavage expression in bovine theca and granulosa cells luteinized by LH or forskolin. Domest Anim Endocrinol, 1998;15:103-114.
McArdle C, Roberson M. Gonadotropes and gonadotropin-releasing hormone signaling. Knobil and Neill’s Physiology of Reproduction, 2015;4:335-397.
Nejad SZ, Tehrani FR, Zadeh-Vakili A. The Role of Kisspeptin in Female Reproduction. Int J Endocrinol Metab, 2017;15(3):e44337. doi: 10.5812/ijem.44337.
Nogueira M, Buratini J, Price C, Castilho A, Pinto M, Barros C. Expression of LH receptor mRNA splice variants in bovine granulosa cells: changes with follicle size and regulation by FSH in vitro. Mol Reprod Dev, 2007;74: 680-686.
Peiró JR, Nogueira GM, Nogueira GP, Perri SH, Cardoso D. Ovariectomy by left flank approach in prepubertal Nelore (Bos indicus) heifers. Can J Vet Res, 2009;73:237-240.
Pierson R, Ginther O. Ultrasonic imaging of the ovaries and uterus in cattle. Theriogenology, 1988;29:21-37.
Rawan A, Yoshioka S, Abe H, Acosta T. Insulin‐Like Growth Factor‐1 Regulates the Expression of Luteinizing Hormone Receptor and Steroid Production in Bovine Granulosa Cells. Reprod Domest Anim, 2015;50:283-291.
Richards J, Hedin L. Molecular aspects of hormone action in ovarian follicular development, ovulation, and luteinization. Annu Rev Physiol, 1988;50: 441-463.
Robert C, Gagne D, Lussier J, Bousquet D, Barnes F, Sirard M. Presence of LH receptor mRNA in granulosa cells as a potential marker of oocyte developmental competence and characterization of the bovine splicing isoforms. Reproduction, 2003;125:437-446.
Senger (2012). Pathways to pregnancy and parturition. 3th ed. Redmond: Current Conceptions. Inc., p.102.
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dc.relation.citationedition.spa.fl_str_mv Núm. 1 , Año 2020
dc.relation.citationendpage.none.fl_str_mv 51
dc.relation.citationissue.spa.fl_str_mv 1
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dc.relation.citationvolume.spa.fl_str_mv 24
dc.relation.ispartofjournal.spa.fl_str_mv Orinoquia
dc.rights.spa.fl_str_mv Orinoquia - 2020
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spelling Vejarano-Orjuela, Álvaro9a9e325cd7e04b3fb05bd865fbb7bd91300Hernández-Vásquez, Aureliano097e588c2335b9f32db6848155a8c109300Rondón-Barragán, Iang S.704e48f83992cb35519df6684f046011Suárez-Mesa, Rafael A.d71b1ba8ebc300bde2e34bc33199cfa53002020-05-11 00:00:002022-06-13T17:42:37Z2020-05-11 00:00:002022-06-13T17:42:37Z2020-05-110121-3709https://repositorio.unillanos.edu.co/handle/001/274310.22579/20112629.5902011-2629https://doi.org/10.22579/20112629.590El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ovario que contenía un folículo preovulatorio, se retiró la pared folicular, se extrajo el RNA total y se sintetizó, amplificó, secuenció y comparó el ADNc contra secuencias ya reportadas, presentando una alta similitud. Se concluye que en estas razas la ausencia de variantes en la expresión del gen, estaría asociada a buen desempeño reproductivo.The luteinizing hormone (LH) choriogonadotropin receptor (LHCGR) gene is highly conserved and its mRNA causes variants by alternative splicing. Variants have been reported in cattle that translate into protein; however, this does not cross the cell membrane. Exon 10 deletion in humans does not trigger a response to the action of LH; it is therefore reasonable to assume that differences regarding response to LH are related to LHR expression. This work was aimed at comparing the Colombian creole Romosinuano breed to the Brahman breed regarding possible HCGR gene mRNA polymorphisms as potential fertility indicators. Five cycling cows were taken from each breed and the ovaries containing pre-ovulatory follicles were excised, the follicular wall was removed and total RNA extracted. The cDNA was synthesised, amplified, sequenced and compared to already reported sequences, great similarity being observed. It was concluded that the lack of variants regarding gene expression in these breeds would be associated with good reproductive performance.application/pdfspaUniversidad de los LlanosOrinoquia - 2020https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/590Inbreedingheterosiscrossingadditivedominantcombining abilityEndogamiaheterosiscruzamientoaditivodominantehabilidad combinatoriaPolimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclandoLuteinizing hormone choriogonadotropin receptor (LHGCR) gene polymorphism in ovarian follicles from cycling Romosinuano and Brahman cowsArtículo de revistaJournal Articleinfo:eu-repo/semantics/articleSección Ciencias agrariasSección Agricultural sciencesinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Texthttp://purl.org/coar/version/c_970fb48d4fbd8a85Cadagan D, Towlson C. Mechanisms of Luteinising Hormone Regulation in Female Steroidogenesis. Am J Med, 2017;5:65-68.Casarini L, Pignatti E, Simoni M. Effects of polymorphisms in gonadotropin and gonadotropin receptor genes on reproductive function. Rev Endocr Metab Dis, 2011;12:303.Clelland E, Peng C. Endocrine/paracrine control of zebrafish ovarian development. Mol Cell Endocrinol, 2009;312:42-52.Delemi A. Identificaion of the expression level to LH-r Gene in dominant and cystic ovarian follicles cells of the cow.Al-Delemi DHJ, Al-Gewary AKA, Ali Jeddoa Z. Identification of the expression level to LH-r gene in dominant and cystic ovarian follicles cells of the cows. J Dairy Vet Anim Res, 2014;1(3):80-86. DOI: 10.15406/jdvar.2014.01.00017Dickinson RE, Stewart AJ, Myers M, Millar RP Duncan WC. Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells: implications for luteolysis. Endocrinology, 2009;150:2873-2881.Gay V, Midgley Jr A, Niswender G. (1970). Patterns of gonadotrophin secretion associated with ovulation. Federation proceedings, p. 1880.Ginther O. Selection of the dominant follicle in cattle and horses. Anim Reprod Sci, 2000;60:61-79.Hastings N, Donn S, Derecka K, Flint A, Woolliams J. Polymorphisms within the coding region of the bovine luteinizing hormone receptor gene and their association with fertility traits. Anim Genet, 2006;37:583-585.Holdridge LR. 1967. Life zone ecology. Life zone ecology.Johnson A, Bridgham J, Wagner B. Characterization of a chicken luteinizing hormone receptor (cLH-R) complementary deoxyribonucleic acid, and expression of cLH-R messenger ribonucleic acid in the ovary. Biol Reprod, 1996;55: 304-309.Kawate N. Studies on the regulation of expression of luteinizing hormone receptor in the ovary and the mechanism of follicular cyst formation in ruminants. J Reprod Develop, 2004;50:1-8.Larsson B. Determination of Ovulation by Ultrasound Examination and its Relation to the LH‐Peak in Heifers. J Vet Med A, 1987;34:749-754.Liu KC, Lin SW, Ge W. Differential regulation of gonadotropin receptors (fshr and lhcgr) by estradiol in the zebrafish ovary involves nuclear estrogen receptors that are likely located on the plasma membrane. Endocrinology, 2011;152:4418-4430.Ma T, Xiong Q, Yuan B, Jiang H, Gao Y, Xu J, Liu S, et al. Luteinizing hormone receptor splicing variants in bovine Leydig cells. Genet Mol Res, 2012;11:1721-1730.Mamluk R, Wolfenson D, Meidan R. LH receptor mRNA and cytochrome P450 side-chain cleavage expression in bovine theca and granulosa cells luteinized by LH or forskolin. Domest Anim Endocrinol, 1998;15:103-114.McArdle C, Roberson M. Gonadotropes and gonadotropin-releasing hormone signaling. Knobil and Neill’s Physiology of Reproduction, 2015;4:335-397.Nejad SZ, Tehrani FR, Zadeh-Vakili A. The Role of Kisspeptin in Female Reproduction. Int J Endocrinol Metab, 2017;15(3):e44337. doi: 10.5812/ijem.44337.Nogueira M, Buratini J, Price C, Castilho A, Pinto M, Barros C. Expression of LH receptor mRNA splice variants in bovine granulosa cells: changes with follicle size and regulation by FSH in vitro. Mol Reprod Dev, 2007;74: 680-686.Peiró JR, Nogueira GM, Nogueira GP, Perri SH, Cardoso D. Ovariectomy by left flank approach in prepubertal Nelore (Bos indicus) heifers. Can J Vet Res, 2009;73:237-240.Pierson R, Ginther O. Ultrasonic imaging of the ovaries and uterus in cattle. Theriogenology, 1988;29:21-37.Rawan A, Yoshioka S, Abe H, Acosta T. Insulin‐Like Growth Factor‐1 Regulates the Expression of Luteinizing Hormone Receptor and Steroid Production in Bovine Granulosa Cells. Reprod Domest Anim, 2015;50:283-291.Richards J, Hedin L. Molecular aspects of hormone action in ovarian follicular development, ovulation, and luteinization. Annu Rev Physiol, 1988;50: 441-463.Robert C, Gagne D, Lussier J, Bousquet D, Barnes F, Sirard M. Presence of LH receptor mRNA in granulosa cells as a potential marker of oocyte developmental competence and characterization of the bovine splicing isoforms. Reproduction, 2003;125:437-446.Senger (2012). Pathways to pregnancy and parturition. 3th ed. Redmond: Current Conceptions. Inc., p.102.https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/590/pdfNúm. 1 , Año 20205114224OrinoquiaPublicationOREORE.xmltext/xml2673https://dspace7-unillanos.metacatalogo.org/bitstreams/faa773dd-6c3c-4eb9-b0f1-690695cdd31c/download24c067f05b27d2979f31d81c9ad5d8ceMD51001/2743oai:dspace7-unillanos.metacatalogo.org:001/27432024-04-17 16:36:54.43https://creativecommons.org/licenses/by/4.0/Orinoquia - 2020metadata.onlyhttps://dspace7-unillanos.metacatalogo.orgRepositorio Universidad de Los Llanosrepositorio@unillanos.edu.co

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