Alternativas de secado de vacas sin antibiótico

La rutina a nivel mundial en el momento del secado de las vacas es el uso de antibióticos de larga duración como profiláctico para que las vacas tengan las lactancias siguientes con la ubre sana. Esta práctica ha funcionado muy bien logrando animales con un conteo de células somáticas muy bajo. Sin...

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Autores:
Rodríguez Monsalve, Laura Isabel
Vargas Mejía, Manuela
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2022
Institución:
Universidad Tecnológica de Pereira
Repositorio:
Repositorio Institucional UTP
Idioma:
spa
OAI Identifier:
oai:repositorio.utp.edu.co:11059/14412
Acceso en línea:
https://hdl.handle.net/11059/14412
https://repositorio.utp.edu.co/home
Palabra clave:
630 - Agricultura y tecnologías relacionadas::636 - Producción animal
Ganado vacuno
Antibioticos en veterinaria
Salud animal
Celulas somáticas
Lactancia
Salud pública
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
id UTP2_709459ca9ae6f3be42cd625c0593e6e4
oai_identifier_str oai:repositorio.utp.edu.co:11059/14412
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network_name_str Repositorio Institucional UTP
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dc.title.spa.fl_str_mv Alternativas de secado de vacas sin antibiótico
dc.title.eng.fl_str_mv Antibiotic-free cow drying alternatives
title Alternativas de secado de vacas sin antibiótico
spellingShingle Alternativas de secado de vacas sin antibiótico
630 - Agricultura y tecnologías relacionadas::636 - Producción animal
Ganado vacuno
Antibioticos en veterinaria
Salud animal
Celulas somáticas
Lactancia
Salud pública
title_short Alternativas de secado de vacas sin antibiótico
title_full Alternativas de secado de vacas sin antibiótico
title_fullStr Alternativas de secado de vacas sin antibiótico
title_full_unstemmed Alternativas de secado de vacas sin antibiótico
title_sort Alternativas de secado de vacas sin antibiótico
dc.creator.fl_str_mv Rodríguez Monsalve, Laura Isabel
Vargas Mejía, Manuela
dc.contributor.advisor.none.fl_str_mv Echeverry López , Juan Carlos
dc.contributor.author.none.fl_str_mv Rodríguez Monsalve, Laura Isabel
Vargas Mejía, Manuela
dc.subject.ddc.none.fl_str_mv 630 - Agricultura y tecnologías relacionadas::636 - Producción animal
topic 630 - Agricultura y tecnologías relacionadas::636 - Producción animal
Ganado vacuno
Antibioticos en veterinaria
Salud animal
Celulas somáticas
Lactancia
Salud pública
dc.subject.lemb.none.fl_str_mv Ganado vacuno
Antibioticos en veterinaria
Salud animal
dc.subject.proposal.spa.fl_str_mv Celulas somáticas
Lactancia
Salud pública
description La rutina a nivel mundial en el momento del secado de las vacas es el uso de antibióticos de larga duración como profiláctico para que las vacas tengan las lactancias siguientes con la ubre sana. Esta práctica ha funcionado muy bien logrando animales con un conteo de células somáticas muy bajo. Sin embargo, uno de los problemas que presenta la humanidad es la resistencia por parte de los microorganismos a los antimicrobianos. El uso desmedido de estos ha llevado a que se presente esta situación generando una problemática de salud pública. La Unión Europea a partir de 2022 prohibió el uso de antibióticos en el momento del secado de las vacas. Esto obliga a crear alternativas que garanticen la salud de la ubre de las vacas. El presente trabajo describe opciones que logren suplir el método que se utilizaba en sistemas anteriores. Estas opciones se buscaron en bases de datos electrónicas del 2018 hasta la fecha. Un sistema empleado por muchos países es el secado selectivo, donde se usa antibiótico solo en vacas más susceptibles con niveles de células somáticas superior a 200.000 por ml y vacas de alta producción. Esto disminuye el uso del antibiótico, pero no desaparece del todo. Otras opciones estudiadas son el uso de selladores y la disminución del ordeño. Hay en estudio opciones que podrían ser empleadas como el uso de extractos de plantas, nanopartículas, bacteriófagos y resinas. Se recomienda realizar trabajos de campo con extractos de plantas que permitan prescindir en un futuro del uso de antibióticos para el secado.
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-12-09T22:15:15Z
dc.date.available.none.fl_str_mv 2022-12-09T22:15:15Z
dc.date.issued.none.fl_str_mv 2022
dc.type.none.fl_str_mv Trabajo de grado - Pregrado
dc.type.version.none.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
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dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/11059/14412
dc.identifier.instname.none.fl_str_mv Universidad Tecnológica de Pereira
dc.identifier.reponame.none.fl_str_mv Repositorio Institucional Universidad Tecnológica de Pereira
dc.identifier.repourl.none.fl_str_mv https://repositorio.utp.edu.co/home
url https://hdl.handle.net/11059/14412
https://repositorio.utp.edu.co/home
identifier_str_mv Universidad Tecnológica de Pereira
Repositorio Institucional Universidad Tecnológica de Pereira
dc.language.iso.none.fl_str_mv spa
language spa
dc.relation.references.none.fl_str_mv Hommels NMC, Ferreira FC, van den Borne BHP, Hogeveen H. Antibiotic use and potential economic impact of implementing selective dry cow therapy in large US dairies. J Dairy Sci [Internet]. 2021;104(8):8931–46. Available from: http://dx.doi.org/10.3168/jds.2020-20016
Zheng Z, Li L, Makhalanyane TP, Xu C, Li K, Xue K, et al. The composition of antibiotic resistance genes is not affected by grazing but is determined by microorganisms in grassland soils. Sci Total Environ [Internet]. 2021;761(19). Available from: https://doi.org/10.1016/j.scitotenv.2020.143205
Gosselin VB, Bodmer M, Schüpbach-Regula G, Steiner A, Meylan M. Survey on the disposal of waste milk containing antimicrobial residues on Swiss dairy farms. J Dairy Sci. 2021;1242–54.
Llanos-Soto SG, Vezeau N, Wemette M, Bulut E, Greiner Safi A, Moroni P, et al. Survey of perceptions and attitudes of an international group of veterinarians regarding antibiotic use and resistance on dairy cattle farms. Prev Vet Med [Internet]. 2021;188. Available from: https://doi.org/10.1016/j.prevetmed.2020.105253
Chida S, Sakamoto M, Takino T, Kawamoto S, Hagiwara K. Changes in immune system and intestinal bacteria of cows during the transition period. Vet Anim Sci [Internet]. 2021;14. Available from: https://doi.org/10.1016/j.vas.2021.100222
Redfern EA, Sinclair LA, Robinson PA. Dairy cow health and management in the transition period: The need to understand the human dimension. Res Vet Sci [Internet]. 2021;137:94–101. Available from: https://doi.org/10.1016/j.rvsc.2021.04.029
Exel CE, Halasa T, Koop G, Steeneveld W, Lam TJGM, Benedictus L, et al. A stochastic modelling approach to determine the effect of diverse Staphylococcus aureus strains on the economic and epidemiological outcomes of mastitis intervention strategies in dairy cattle. Prev Vet Med [Internet]. 2021;199. Available from: https://doi.org/10.1016/j.prevetmed.2021.105566
McAloon CI, McCoy F, More SJ. Trends in estimated intramammary antimicrobial usage in the Irish dairy industry from 2003 to 2019. JDS Commun [Internet]. 2021;2(5):271–6. Available from: http://dx.doi.org/10.3168/jdsc.2021- 0081
Krogh MA, Nielsen CL, Sørensen JT. Antimicrobial use in organic and conventional dairy herds. Animal [Internet]. 2020;14(10):2187–93. Available from: http://dx.doi.org/10.1017/S1751731120000920
Cattaneo L, Piccioli-Cappelli F, Lopreiato V, Lovotti G, Arrigoni N, Minuti A, et al. Drying-off cows with low somatic cell count with or without antibiotic therapy: A pilot study addressing the effects on immunometabolism and performance in the subsequent lactation. Livest Sci [Internet]. 2021;254. Available from: https://doi.org/10.1016/j.livsci.2021.104740
Igualada C, Giraldo J, Font G, Yusà V. Validation of a multi-residue UHPLC HRMS method for antibiotics screening in milk, fresh cheese, and whey. J Food Compos Anal. 2022;106.
Bir C, Widmar NO, Thompson NM, Townsend J, Wolf CA. US respondents’ willingness to pay for Cheddar cheese from dairy cattle with different pasture access, antibiotic use, and dehorning practices. J Dairy Sci [Internet]. 2020;103(4):3234–49. Available from: http://dx.doi.org/10.3168/jds.2019-17031
Dankar I, Hassan H, Serhan M. Knowledge, attitudes, and perceptions of dairy farmers regarding antibiotic use: Lessons from a developing country. J Dairy Sci [Internet]. 2022;105(2):1519–32. Available from: http://dx.doi.org/10.3168/jds.2021-20951
Niemi RE, Hovinen M, Vilar MJ, Simojoki H, Rajala-Schultz PJ. Dry cow therapy and early lactation udder health problems—Associations and risk factors. Prev Vet Med [Internet]. 2021;188. Available from: https://doi.org/10.1016/j.prevetmed.2021.105268
Lopes TS, Fontoura PS, Oliveira A, Rizzo FA, Silveira S, Streck AF. Use of plant extracts and essential oils in the control of bovine mastitis. Res Vet Sci [Internet]. 2020;131:186–93. Available from: https://doi.org/10.1016/j.rvsc.2020.04.025
Alhussien MN, Tiwari S, Panda BSK, Pandey Y, Lathwal SS, Dang AK. Supplementation of antioxidant micronutrients reduces stress and improves immune function/response in periparturient dairy cows and their calves. J Trace Elem Med Biol [Internet]. 2021;65. Available from: https://doi.org/10.1016/j.jtemb.2021.126718
Niemi RE, Hovinen M, Rajala-Schultz PJ. Selective dry cow therapy effect on milk yield and somatic cell count: A retrospective cohort study. J Dairy Sci [Internet]. 2022;105(2):1387–401. Available from: http://dx.doi.org/10.3168/jds.2021-20918
Jariyasopit N, Khamsaeng S, Panya A, Vinaisuratern P, Metem P, Asawalertpanich W, et al. Quantitative analysis of nutrient metabolite compositions of retail cow’s milk and milk alternatives in Thailand using GC-MS. J Food Compos Anal. 2021;97.
Britt JH, Cushman RA, Dechow CD, Dobson H, Humblot P, Hutjens MF, et al. Review: Perspective on high-performing dairy cows and herds. Animal [Internet]. 2021;15. Available from: https://doi.org/10.1016/j.animal.2021.100298
Helliwell R, Morris C, Raman S. Antibiotic stewardship and its implications for agricultural animal-human relationships: Insights from an intensive dairy farm in England. J Rural Stud [Internet]. 2020;78:447–56. Available from:https://doi.org/10.1016/j.jrurstud.2020.07.008
Castillo-Aguirre A, Cañas A, Honda L, Richter P. Determination of veterinary antibiotics in cow milk using rotating-disk sorptive extraction and liquid chromatography. Microchem J. 2021;162
Rees GM, Reyher KK, Barrett DC, Buller H. ‘It’s cheaper than a dead cow’: Understanding veterinary medicine use on dairy farms. J Rural Stud. 2021;86:587–98.
Hennessey M, Whatford L, Payne-Gifford S, Johnson KF, Van Winden S, Barling D, et al. Antimicrobial & antiparasitic use and resistance in British sheep and cattle: a systematic review. Prev Vet Med. 2020;185.
Bagheri AR, Ghaedi M. Magnetic metal organic framework for pre-concentration of ampicillin from cow milk samples. J Pharm Anal [Internet]. 2020;10(4):365– 75. Available from: https://doi.org/10.1016/j.jpha.2020.02.006
Carolina Rafanhin Sousa A, Nascimento Makara C, Canniatti Brazaca L, Carrilho E. A colorimetric microfluidic paper-based analytical device for sulfonamides in cow milk using enzymatic inhibition. Food Chem [Internet]. 2021;356. Available from: https://doi.org/10.1016/j.foodchem.2021.129692
Zhang L, Li L, Sha G, Liu C, Wang Z, Wang L. Aerobic composting as an effective cow manure management strategy for reducing the dissemination of antibiotic resistance genes: An integrated meta-omics study. J Hazard Mater [Internet]. 2020;386. Available from: https://doi.org/10.1016/j.jhazmat.2019.121895
McBride SG, Wepking C, Hedin ML, Thompson RC, Barrett JE, Strickland MS. Livestock manure and antibiotics alter extracellular enzyme activity. Appl Soil Ecol [Internet]. 2020;155. Available from: https://doi.org/10.1016/j.apsoil.2020.103667
Ezugworie FN, Igbokwe VC, Onwosi CO. Proliferation of antibiotic-resistant microorganisms and associated genes during composting: An overview of the potential impacts on public health, management and future. Sci Total Environ [Internet]. 2021;784. Available from: https://doi.org/10.1016/j.scitotenv.2021.147191
Paudyal S, Manriquez D, Velasquez A, Shearer JK, Plummer PJ, Melendez P, et al. Efficacy of non-antibiotic treatment options for digital dermatitis on an organic dairy farm. Vet J [Internet]. 2020;255. Available from: https://doi.org/10.1016/j.tvjl.2019.105417
Bonestroo J, Fall N, van der Voort M, Klaas IC, Hogeveen H, Emanuelson U. Diagnostic properties of milk diversion and farmer-reported mastitis to indicate clinical mastitis status in dairy cows using Bayesian latent class analysis. Livest Sci [Internet]. 2021;253. Available from: https://doi.org/10.1016/j.livsci.2021.104698
Vilar MJ, Rajala-Schultz PJ. Dry-off and dairy cow udder health and welfare: Effects of different milk cessation methods. Vet J [Internet]. 2020;262. Available from: https://doi.org/10.1016/j.tvjl.2020.105503
Rowe S, Godden S, Nydam D V., Gorden P, Lago A, Vasquez A, et al. Evaluation of rapid culture, a predictive algorithm, esterase somatic cell count and lactate dehydrogenase to detect intramammary infection in quarters of dairy cows at dry-off. Prev Vet Med [Internet]. 2020;179. Available from: https://doi.org/10.1016/j.prevetmed.2020.104982
Georgakakos CB, Hicks BJ, Walter MT. Farmer perceptions of dairy farm antibiotic use and transport pathways as determinants of contaminant loads to the environment. J Environ Manage [Internet]. 2021;281. Available from: https://doi.org/10.1016/j.jenvman.2020.111880
Wilms JN, Hare KS, Fischer-Tlustos AJ, Vahmani P, Dugan MER, Leal LN, et al. Fatty acid profile characterization in colostrum, transition milk, and mature milk of primi- and multiparous cows during the first week of lactation. J Dairy Sci [Internet]. 2022; Available from: http://dx.doi.org/10.3168/jds.2021-20880
Silva SNS e., Chabrillat T, Kerros S, Guillaume S, Gandra JR, de Carvalho GGP, et al. Effects of plant extract supplementations or monensin on nutrient intake, digestibility, ruminal fermentation and metabolism in dairy cows. Anim Feed Sci Technol [Internet]. 2021;275. Available from: https://doi.org/10.1016/j.anifeedsci.2021.114886
Li XP, Tan ZL, Li ZC, Gao S, Yi KL, Zhou CS, et al. Metabolomic changes in the liver tissues of cows in early lactation supplemented with dietary rumen protected glucose during the transition period. Anim Feed Sci Technol. 2021;281.
Torres RNS, Paschoaloto JR, Ezequiel JMB, da Silva DAV, Almeida MTC. Meta analysis of the effects of essential oil as an alternative to monensin in diets for beef cattle. Vet J [Internet]. 2021;272. Available from: https://doi.org/10.1016/j.tvjl.2021.105659
Hardie LC, Haagen IW, Heins BJ, Dechow CD. Genetic parameters and association of national evaluations with breeding values for health traits in US organic Holstein cows. J Dairy Sci [Internet]. 2022;105(1):495–508. Available from: http://dx.doi.org/10.3168/jds.2021-20588
McClearn B, Delaby L, Gilliland TJ, Guy C, Dineen M, Coughlan F, et al. An assessment of the production, reproduction, and functional traits of Holstein Friesian, Jersey × Holstein-Friesian, and Norwegian Red × (Jersey × Holstein Friesian) cows in pasture-based systems. J Dairy Sci [Internet]. 2020;103(6):5200–14. Available from: http://dx.doi.org/10.3168/jds.2019-17476
Clabby C, Mcparland S, Dillon P, Arkins S, Flynn J, Murphy J, et al. Animal The international journal of animal biosciences Internal teat sealants alone or in combination with antibiotics at dry-off – the effect on udder health in dairy cows in five commercial herds. Animal [Internet]. 2022;16(2). Available from: https://doi.org/10.1016/j.animal.2021.100449
Regan Á, Clifford S, Burrell AMG, Balaine L, Dillon E. Exploring the relationship between mastitis risk perceptions and farmers’ readiness to engage in milk recording. Prev Vet Med. 2021;193.
Vasquez A, Nydam D, Foditsch C, Warnick L, Wolfe C, Doster E, et al. Characterization and comparison of the microbiomes and resistomes of colostrum from selectively treated dry cows. J Dairy Sci [Internet] 2022;105(1):637–53. Available from: http://dx.doi.org/10.3168/jds.2021-20675
Niedziela DA, Murphy MP, Grant J, Keane OM, Leonard FC. Clinical presentation and immune characteristics in first-lactation Holstein-Friesian cows following intramammary infection with genotypically distinct Staphylococcus aureus strains. J Dairy Sci [Internet]. 2020;103(9):8453–66. Available from: http://dx.doi.org/10.3168/jds.2019-17433
Rowe SM, Godden SM, Nydam D V., Lago A, Vasquez AK, Royster E, et al. Randomized equivalence study comparing the efficacy of 2 commercial internal teat sealants in dairy cows. J Dairy Sci [Internet]. 2020;103(6):5398–413. Available from: http://dx.doi.org/10.3168/jds.2019-17884
Zhang H min, Jiang H rui, Chen D jie, Shen Z liang, Mao Y jiang, Liang Y sheng, et al. Evaluation of a povidone-iodine and chitosan-based barrier teat dip in the prevention of mastitis in dairy cows. J Integr Agric [Internet]. 2021;20(6):1615– 25. Available from: http://dx.doi.org/10.1016/S2095-3119(20)63418-9
Kabera F, Dufour S, Keefe G, Cameron M, Roy JP. Evaluation of quarter-based selective dry cow therapy using Petrifilm on-farm milk culture: A randomized controlled trial. J Dairy Sci. 2020;103(8):7276–87.
Swinkels JM, Leach KA, Breen JE, Payne B, White V, Green MJ, et al. Randomized controlled field trial comparing quarter and cow level selective dry cow treatment using the California Mastitis Test. J Dairy Sci [Internet]. 2021;104(8):9063–81. Available from: http://dx.doi.org/10.3168/jds.2020-19258
Mcdougall S. Bacteriological outcomes following random allocation to quarter level selection based on California Mastitis Test score or cow-level allocation based on somatic cell count for dry cow therapy. J Dairy Sci [Internet]. 2022; Available from: http://dx.doi.org/10.3168/jds.2021-21020
Fan P, Ma Z, Partow AJ, Kim M, Shoemaker GM, Tan R, et al. A novel combination therapy for multidrug resistant pathogens using chitosan nanoparticles loaded with β-lactam antibiotics and β-lactamase inhibitors. Int J Biol Macromol [Internet]. 2022;195:506–14. Available from: https://doi.org/10.1016/j.ijbiomac.2021.12.035
Ster C, Allard M, Côté-Gravel J, Boulanger S, Lacasse P, Malouin F. Immune and experimental infection responses of dairy cows vaccinated with the combination of six Staphylococcus aureus proteins that are expressed during bovine intramammary infection and a triple adjuvant. Vet Immunol Immunopathol. 2021;238.
Martin LM, Sauerwein H, Büscher W, Müller U. Automated gradual reduction of milk yield before dry-off: Effects on udder health, involution and inner teat morphology. Livest Sci [Internet]. 2020;233. Available from: https://doi.org/10.1016/j.livsci.2020.103942
Schlabitz C, Lehn DN, Volken de Souza CF. A review of Saccharomyces cerevisiae and the applications of its byproducts in dairy cattle feed: Trends in the use of residual brewer’s yeast. J Clean Prod. 2021;332.
Barreto MO, Soust M, Moore RJ, Olchowy TWJ, Alawneh JI. Systematic review and meta-analysis of probiotic use on inflammatory biomarkers and disease prevention in cattle. Prev Vet Med [Internet]. 2021;194. Available from: https://doi.org/10.1016/j.prevetmed.2021.105433
Zaatout N. An overview on mastitis-associated Escherichia coli: Pathogenicity, host immunity and the use of alternative therapies. Microbiol Res [Internet]. 2022;256. Available from: https://doi.org/10.1016/j.micres.2021.126960
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)Manifiesto (Manifestamos) en este documento la voluntad de autorizar a la Biblioteca Jorge Roa Martínez de la Universidad Tecnológica de Pereira la publicación en el Repositorio institucional (http://biblioteca.utp.edu.co), la versión electrónica de la OBRA titulada: ________________________________________________________________________________________________ ________________________________________________________________________________________________ ________________________________________________________________________________________________ La Universidad Tecnológica de Pereira, entidad académica sin ánimo de lucro, queda por lo tanto facultada para ejercer plenamente la autorización anteriormente descrita en su actividad ordinaria de investigación, docencia y publicación. La autorización otorgada se ajusta a lo que establece la Ley 23 de 1982. Con todo, en mi (nuestra) condición de autor (es) me (nos) reservo (reservamos) los derechos morales de la OBRA antes citada con arreglo al artículo 30 dehttps://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessEcheverry López , Juan CarlosRodríguez Monsalve, Laura IsabelVargas Mejía, Manuela2022-12-09T22:15:15Z2022-12-09T22:15:15Z2022https://hdl.handle.net/11059/14412Universidad Tecnológica de PereiraRepositorio Institucional Universidad Tecnológica de Pereirahttps://repositorio.utp.edu.co/homeLa rutina a nivel mundial en el momento del secado de las vacas es el uso de antibióticos de larga duración como profiláctico para que las vacas tengan las lactancias siguientes con la ubre sana. Esta práctica ha funcionado muy bien logrando animales con un conteo de células somáticas muy bajo. Sin embargo, uno de los problemas que presenta la humanidad es la resistencia por parte de los microorganismos a los antimicrobianos. El uso desmedido de estos ha llevado a que se presente esta situación generando una problemática de salud pública. La Unión Europea a partir de 2022 prohibió el uso de antibióticos en el momento del secado de las vacas. Esto obliga a crear alternativas que garanticen la salud de la ubre de las vacas. El presente trabajo describe opciones que logren suplir el método que se utilizaba en sistemas anteriores. Estas opciones se buscaron en bases de datos electrónicas del 2018 hasta la fecha. Un sistema empleado por muchos países es el secado selectivo, donde se usa antibiótico solo en vacas más susceptibles con niveles de células somáticas superior a 200.000 por ml y vacas de alta producción. Esto disminuye el uso del antibiótico, pero no desaparece del todo. Otras opciones estudiadas son el uso de selladores y la disminución del ordeño. Hay en estudio opciones que podrían ser empleadas como el uso de extractos de plantas, nanopartículas, bacteriófagos y resinas. Se recomienda realizar trabajos de campo con extractos de plantas que permitan prescindir en un futuro del uso de antibióticos para el secado.The worldwide routine at the time of cow dry-off is the use of long term antibiotics as a prophylactic to ensure that cows have healthy udders in the following lactations. This practice has worked very well in achieving animals with very low somatic cell counts. However, one of the problems of mankind is the resistance of microorganisms to antimicrobials. The excessive use of antimicrobials has led to this situation generating a public health problem. As of 2022, the European Union banned the use of antibiotics at the time of drying cows. This makes it necessary to create alternatives that guarantee the health of the cows' udder. The present work describes options to replace the method used in previous systems. These options were searched in electronic databases from 2018 to date. One system employed by many countries is selective drying off, where antibiotic is used only on more susceptible cows with somatic cell levels above 200,000 per ml and high producing cows. This reduces antibiotic use, but does not eliminate it completely. Other options being studied are the use of sealants and decreased milking. There are options under study that could be employed such as the use of plant extracts, nanoparticles, bacteriophages and resins. It is recommended to carry out field work with plant extracts that will make it possible to dispense with the use of antibiotics for drying in the future.PregradoMédico(a) Veterinario(a) y Zootecnista18 Páginasapplication/pdfspaUniversidad Tecnológica de PereiraMedicina Veterinaria y ZootecniaFacultad de Ciencias de la SaludPereira630 - Agricultura y tecnologías relacionadas::636 - Producción animalGanado vacunoAntibioticos en veterinariaSalud animalCelulas somáticasLactanciaSalud públicaAlternativas de secado de vacas sin antibióticoAntibiotic-free cow drying alternativesTrabajo de grado - Pregradoinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_7a1fTextinfo:eu-repo/semantics/bachelorThesisHommels NMC, Ferreira FC, van den Borne BHP, Hogeveen H. Antibiotic use and potential economic impact of implementing selective dry cow therapy in large US dairies. J Dairy Sci [Internet]. 2021;104(8):8931–46. Available from: http://dx.doi.org/10.3168/jds.2020-20016Zheng Z, Li L, Makhalanyane TP, Xu C, Li K, Xue K, et al. The composition of antibiotic resistance genes is not affected by grazing but is determined by microorganisms in grassland soils. Sci Total Environ [Internet]. 2021;761(19). Available from: https://doi.org/10.1016/j.scitotenv.2020.143205Gosselin VB, Bodmer M, Schüpbach-Regula G, Steiner A, Meylan M. Survey on the disposal of waste milk containing antimicrobial residues on Swiss dairy farms. J Dairy Sci. 2021;1242–54.Llanos-Soto SG, Vezeau N, Wemette M, Bulut E, Greiner Safi A, Moroni P, et al. Survey of perceptions and attitudes of an international group of veterinarians regarding antibiotic use and resistance on dairy cattle farms. Prev Vet Med [Internet]. 2021;188. Available from: https://doi.org/10.1016/j.prevetmed.2020.105253Chida S, Sakamoto M, Takino T, Kawamoto S, Hagiwara K. Changes in immune system and intestinal bacteria of cows during the transition period. Vet Anim Sci [Internet]. 2021;14. Available from: https://doi.org/10.1016/j.vas.2021.100222Redfern EA, Sinclair LA, Robinson PA. Dairy cow health and management in the transition period: The need to understand the human dimension. Res Vet Sci [Internet]. 2021;137:94–101. Available from: https://doi.org/10.1016/j.rvsc.2021.04.029Exel CE, Halasa T, Koop G, Steeneveld W, Lam TJGM, Benedictus L, et al. A stochastic modelling approach to determine the effect of diverse Staphylococcus aureus strains on the economic and epidemiological outcomes of mastitis intervention strategies in dairy cattle. Prev Vet Med [Internet]. 2021;199. Available from: https://doi.org/10.1016/j.prevetmed.2021.105566McAloon CI, McCoy F, More SJ. Trends in estimated intramammary antimicrobial usage in the Irish dairy industry from 2003 to 2019. JDS Commun [Internet]. 2021;2(5):271–6. Available from: http://dx.doi.org/10.3168/jdsc.2021- 0081Krogh MA, Nielsen CL, Sørensen JT. Antimicrobial use in organic and conventional dairy herds. Animal [Internet]. 2020;14(10):2187–93. Available from: http://dx.doi.org/10.1017/S1751731120000920Cattaneo L, Piccioli-Cappelli F, Lopreiato V, Lovotti G, Arrigoni N, Minuti A, et al. Drying-off cows with low somatic cell count with or without antibiotic therapy: A pilot study addressing the effects on immunometabolism and performance in the subsequent lactation. Livest Sci [Internet]. 2021;254. Available from: https://doi.org/10.1016/j.livsci.2021.104740Igualada C, Giraldo J, Font G, Yusà V. Validation of a multi-residue UHPLC HRMS method for antibiotics screening in milk, fresh cheese, and whey. J Food Compos Anal. 2022;106.Bir C, Widmar NO, Thompson NM, Townsend J, Wolf CA. US respondents’ willingness to pay for Cheddar cheese from dairy cattle with different pasture access, antibiotic use, and dehorning practices. J Dairy Sci [Internet]. 2020;103(4):3234–49. Available from: http://dx.doi.org/10.3168/jds.2019-17031Dankar I, Hassan H, Serhan M. Knowledge, attitudes, and perceptions of dairy farmers regarding antibiotic use: Lessons from a developing country. J Dairy Sci [Internet]. 2022;105(2):1519–32. Available from: http://dx.doi.org/10.3168/jds.2021-20951Niemi RE, Hovinen M, Vilar MJ, Simojoki H, Rajala-Schultz PJ. Dry cow therapy and early lactation udder health problems—Associations and risk factors. Prev Vet Med [Internet]. 2021;188. Available from: https://doi.org/10.1016/j.prevetmed.2021.105268Lopes TS, Fontoura PS, Oliveira A, Rizzo FA, Silveira S, Streck AF. Use of plant extracts and essential oils in the control of bovine mastitis. Res Vet Sci [Internet]. 2020;131:186–93. Available from: https://doi.org/10.1016/j.rvsc.2020.04.025Alhussien MN, Tiwari S, Panda BSK, Pandey Y, Lathwal SS, Dang AK. Supplementation of antioxidant micronutrients reduces stress and improves immune function/response in periparturient dairy cows and their calves. J Trace Elem Med Biol [Internet]. 2021;65. Available from: https://doi.org/10.1016/j.jtemb.2021.126718Niemi RE, Hovinen M, Rajala-Schultz PJ. Selective dry cow therapy effect on milk yield and somatic cell count: A retrospective cohort study. J Dairy Sci [Internet]. 2022;105(2):1387–401. Available from: http://dx.doi.org/10.3168/jds.2021-20918Jariyasopit N, Khamsaeng S, Panya A, Vinaisuratern P, Metem P, Asawalertpanich W, et al. Quantitative analysis of nutrient metabolite compositions of retail cow’s milk and milk alternatives in Thailand using GC-MS. J Food Compos Anal. 2021;97.Britt JH, Cushman RA, Dechow CD, Dobson H, Humblot P, Hutjens MF, et al. Review: Perspective on high-performing dairy cows and herds. Animal [Internet]. 2021;15. Available from: https://doi.org/10.1016/j.animal.2021.100298Helliwell R, Morris C, Raman S. Antibiotic stewardship and its implications for agricultural animal-human relationships: Insights from an intensive dairy farm in England. J Rural Stud [Internet]. 2020;78:447–56. Available from:https://doi.org/10.1016/j.jrurstud.2020.07.008Castillo-Aguirre A, Cañas A, Honda L, Richter P. Determination of veterinary antibiotics in cow milk using rotating-disk sorptive extraction and liquid chromatography. Microchem J. 2021;162Rees GM, Reyher KK, Barrett DC, Buller H. ‘It’s cheaper than a dead cow’: Understanding veterinary medicine use on dairy farms. J Rural Stud. 2021;86:587–98.Hennessey M, Whatford L, Payne-Gifford S, Johnson KF, Van Winden S, Barling D, et al. Antimicrobial & antiparasitic use and resistance in British sheep and cattle: a systematic review. Prev Vet Med. 2020;185.Bagheri AR, Ghaedi M. Magnetic metal organic framework for pre-concentration of ampicillin from cow milk samples. J Pharm Anal [Internet]. 2020;10(4):365– 75. Available from: https://doi.org/10.1016/j.jpha.2020.02.006Carolina Rafanhin Sousa A, Nascimento Makara C, Canniatti Brazaca L, Carrilho E. A colorimetric microfluidic paper-based analytical device for sulfonamides in cow milk using enzymatic inhibition. Food Chem [Internet]. 2021;356. Available from: https://doi.org/10.1016/j.foodchem.2021.129692Zhang L, Li L, Sha G, Liu C, Wang Z, Wang L. Aerobic composting as an effective cow manure management strategy for reducing the dissemination of antibiotic resistance genes: An integrated meta-omics study. J Hazard Mater [Internet]. 2020;386. Available from: https://doi.org/10.1016/j.jhazmat.2019.121895McBride SG, Wepking C, Hedin ML, Thompson RC, Barrett JE, Strickland MS. Livestock manure and antibiotics alter extracellular enzyme activity. Appl Soil Ecol [Internet]. 2020;155. Available from: https://doi.org/10.1016/j.apsoil.2020.103667Ezugworie FN, Igbokwe VC, Onwosi CO. Proliferation of antibiotic-resistant microorganisms and associated genes during composting: An overview of the potential impacts on public health, management and future. Sci Total Environ [Internet]. 2021;784. Available from: https://doi.org/10.1016/j.scitotenv.2021.147191Paudyal S, Manriquez D, Velasquez A, Shearer JK, Plummer PJ, Melendez P, et al. Efficacy of non-antibiotic treatment options for digital dermatitis on an organic dairy farm. Vet J [Internet]. 2020;255. Available from: https://doi.org/10.1016/j.tvjl.2019.105417Bonestroo J, Fall N, van der Voort M, Klaas IC, Hogeveen H, Emanuelson U. Diagnostic properties of milk diversion and farmer-reported mastitis to indicate clinical mastitis status in dairy cows using Bayesian latent class analysis. Livest Sci [Internet]. 2021;253. Available from: https://doi.org/10.1016/j.livsci.2021.104698Vilar MJ, Rajala-Schultz PJ. Dry-off and dairy cow udder health and welfare: Effects of different milk cessation methods. Vet J [Internet]. 2020;262. Available from: https://doi.org/10.1016/j.tvjl.2020.105503Rowe S, Godden S, Nydam D V., Gorden P, Lago A, Vasquez A, et al. Evaluation of rapid culture, a predictive algorithm, esterase somatic cell count and lactate dehydrogenase to detect intramammary infection in quarters of dairy cows at dry-off. Prev Vet Med [Internet]. 2020;179. Available from: https://doi.org/10.1016/j.prevetmed.2020.104982Georgakakos CB, Hicks BJ, Walter MT. Farmer perceptions of dairy farm antibiotic use and transport pathways as determinants of contaminant loads to the environment. J Environ Manage [Internet]. 2021;281. Available from: https://doi.org/10.1016/j.jenvman.2020.111880Wilms JN, Hare KS, Fischer-Tlustos AJ, Vahmani P, Dugan MER, Leal LN, et al. Fatty acid profile characterization in colostrum, transition milk, and mature milk of primi- and multiparous cows during the first week of lactation. J Dairy Sci [Internet]. 2022; Available from: http://dx.doi.org/10.3168/jds.2021-20880Silva SNS e., Chabrillat T, Kerros S, Guillaume S, Gandra JR, de Carvalho GGP, et al. Effects of plant extract supplementations or monensin on nutrient intake, digestibility, ruminal fermentation and metabolism in dairy cows. Anim Feed Sci Technol [Internet]. 2021;275. Available from: https://doi.org/10.1016/j.anifeedsci.2021.114886Li XP, Tan ZL, Li ZC, Gao S, Yi KL, Zhou CS, et al. Metabolomic changes in the liver tissues of cows in early lactation supplemented with dietary rumen protected glucose during the transition period. Anim Feed Sci Technol. 2021;281.Torres RNS, Paschoaloto JR, Ezequiel JMB, da Silva DAV, Almeida MTC. Meta analysis of the effects of essential oil as an alternative to monensin in diets for beef cattle. Vet J [Internet]. 2021;272. Available from: https://doi.org/10.1016/j.tvjl.2021.105659Hardie LC, Haagen IW, Heins BJ, Dechow CD. Genetic parameters and association of national evaluations with breeding values for health traits in US organic Holstein cows. J Dairy Sci [Internet]. 2022;105(1):495–508. Available from: http://dx.doi.org/10.3168/jds.2021-20588McClearn B, Delaby L, Gilliland TJ, Guy C, Dineen M, Coughlan F, et al. An assessment of the production, reproduction, and functional traits of Holstein Friesian, Jersey × Holstein-Friesian, and Norwegian Red × (Jersey × Holstein Friesian) cows in pasture-based systems. J Dairy Sci [Internet]. 2020;103(6):5200–14. Available from: http://dx.doi.org/10.3168/jds.2019-17476Clabby C, Mcparland S, Dillon P, Arkins S, Flynn J, Murphy J, et al. Animal The international journal of animal biosciences Internal teat sealants alone or in combination with antibiotics at dry-off – the effect on udder health in dairy cows in five commercial herds. Animal [Internet]. 2022;16(2). Available from: https://doi.org/10.1016/j.animal.2021.100449Regan Á, Clifford S, Burrell AMG, Balaine L, Dillon E. Exploring the relationship between mastitis risk perceptions and farmers’ readiness to engage in milk recording. Prev Vet Med. 2021;193.Vasquez A, Nydam D, Foditsch C, Warnick L, Wolfe C, Doster E, et al. Characterization and comparison of the microbiomes and resistomes of colostrum from selectively treated dry cows. J Dairy Sci [Internet] 2022;105(1):637–53. Available from: http://dx.doi.org/10.3168/jds.2021-20675Niedziela DA, Murphy MP, Grant J, Keane OM, Leonard FC. Clinical presentation and immune characteristics in first-lactation Holstein-Friesian cows following intramammary infection with genotypically distinct Staphylococcus aureus strains. J Dairy Sci [Internet]. 2020;103(9):8453–66. Available from: http://dx.doi.org/10.3168/jds.2019-17433Rowe SM, Godden SM, Nydam D V., Lago A, Vasquez AK, Royster E, et al. Randomized equivalence study comparing the efficacy of 2 commercial internal teat sealants in dairy cows. J Dairy Sci [Internet]. 2020;103(6):5398–413. Available from: http://dx.doi.org/10.3168/jds.2019-17884Zhang H min, Jiang H rui, Chen D jie, Shen Z liang, Mao Y jiang, Liang Y sheng, et al. Evaluation of a povidone-iodine and chitosan-based barrier teat dip in the prevention of mastitis in dairy cows. J Integr Agric [Internet]. 2021;20(6):1615– 25. Available from: http://dx.doi.org/10.1016/S2095-3119(20)63418-9Kabera F, Dufour S, Keefe G, Cameron M, Roy JP. Evaluation of quarter-based selective dry cow therapy using Petrifilm on-farm milk culture: A randomized controlled trial. J Dairy Sci. 2020;103(8):7276–87.Swinkels JM, Leach KA, Breen JE, Payne B, White V, Green MJ, et al. Randomized controlled field trial comparing quarter and cow level selective dry cow treatment using the California Mastitis Test. J Dairy Sci [Internet]. 2021;104(8):9063–81. Available from: http://dx.doi.org/10.3168/jds.2020-19258Mcdougall S. Bacteriological outcomes following random allocation to quarter level selection based on California Mastitis Test score or cow-level allocation based on somatic cell count for dry cow therapy. J Dairy Sci [Internet]. 2022; Available from: http://dx.doi.org/10.3168/jds.2021-21020Fan P, Ma Z, Partow AJ, Kim M, Shoemaker GM, Tan R, et al. A novel combination therapy for multidrug resistant pathogens using chitosan nanoparticles loaded with β-lactam antibiotics and β-lactamase inhibitors. Int J Biol Macromol [Internet]. 2022;195:506–14. Available from: https://doi.org/10.1016/j.ijbiomac.2021.12.035Ster C, Allard M, Côté-Gravel J, Boulanger S, Lacasse P, Malouin F. Immune and experimental infection responses of dairy cows vaccinated with the combination of six Staphylococcus aureus proteins that are expressed during bovine intramammary infection and a triple adjuvant. Vet Immunol Immunopathol. 2021;238.Martin LM, Sauerwein H, Büscher W, Müller U. Automated gradual reduction of milk yield before dry-off: Effects on udder health, involution and inner teat morphology. Livest Sci [Internet]. 2020;233. Available from: https://doi.org/10.1016/j.livsci.2020.103942Schlabitz C, Lehn DN, Volken de Souza CF. A review of Saccharomyces cerevisiae and the applications of its byproducts in dairy cattle feed: Trends in the use of residual brewer’s yeast. J Clean Prod. 2021;332.Barreto MO, Soust M, Moore RJ, Olchowy TWJ, Alawneh JI. Systematic review and meta-analysis of probiotic use on inflammatory biomarkers and disease prevention in cattle. Prev Vet Med [Internet]. 2021;194. Available from: https://doi.org/10.1016/j.prevetmed.2021.105433Zaatout N. An overview on mastitis-associated Escherichia coli: Pathogenicity, host immunity and the use of alternative therapies. Microbiol Res [Internet]. 2022;256. Available from: https://doi.org/10.1016/j.micres.2021.126960PublicationORIGINALTRABAJO DE GRADO.pdfTRABAJO DE GRADO.pdfapplication/pdf125643https://dspace7-utp.metabuscador.org/bitstreams/cf681d00-a929-4a01-9e9f-b9bd5a111668/download16b7d892ac306760d04a0d22ae8c209fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-814828https://dspace7-utp.metabuscador.org/bitstreams/c6340ca9-e18d-4d53-aef5-4f7f87a5f5f3/download2f9959eaf5b71fae44bbf9ec84150c7aMD52TEXTTRABAJO DE GRADO.pdf.txtTRABAJO DE GRADO.pdf.txtExtracted texttext/plain39233https://dspace7-utp.metabuscador.org/bitstreams/1345cf25-d00b-480b-8cc6-7fafa0f97d37/download8a2c55fdac7826ea998e5af73f9d5448MD53THUMBNAILTRABAJO DE GRADO.pdf.jpgTRABAJO DE GRADO.pdf.jpgGenerated Thumbnailimage/jpeg13190https://dspace7-utp.metabuscador.org/bitstreams/34e7ae28-e72c-4d01-91e9-0f0031de2da5/download118c1255bca5fb0c983428e5cd92b8ddMD5411059/14412oai:dspace7-utp.metabuscador.org:11059/144122024-09-05 16:45:46.084https://creativecommons.org/licenses/by-nc-nd/4.0/Manifiesto (Manifestamos) en este documento la voluntad de autorizar a la Biblioteca Jorge Roa Martínez de la Universidad Tecnológica de Pereira la publicación en el Repositorio institucional (http://biblioteca.utp.edu.co), la versión electrónica de la OBRA titulada: ________________________________________________________________________________________________ ________________________________________________________________________________________________ ________________________________________________________________________________________________ La Universidad Tecnológica de Pereira, entidad académica sin ánimo de lucro, queda por lo tanto facultada para ejercer plenamente la autorización anteriormente descrita en su actividad ordinaria de investigación, docencia y publicación. La autorización otorgada se ajusta a lo que establece la Ley 23 de 1982. Con todo, en mi (nuestra) condición de autor (es) me (nos) reservo (reservamos) los derechos morales de la OBRA antes citada con arreglo al artículo 30 deopen.accesshttps://dspace7-utp.metabuscador.orgRepositorio de la Universidad Tecnológica de 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