Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries
It is essential to understand bacterial succession during the development of dental caries and determine the microorganisms related to this disease. This work aimed to identify bacteria associated with cariogenic pieces and carry out an antibiogram assay. First, we isolated bacterial isolates using...
- Autores:
-
Jimenez-Ramirez, Verónica Alejandra
Chavarro Mesa, Edisson
Acevedo Barrios, Rosa Leonor
Ariza Rúa, Danilo Lusbin
Martínez Santos, Juan Carlos
- Tipo de recurso:
- http://purl.org/coar/resource_type/c_c94f
- Fecha de publicación:
- 2023
- Institución:
- Universidad Tecnológica de Bolívar
- Repositorio:
- Repositorio Institucional UTB
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.utb.edu.co:20.500.12585/12586
- Acceso en línea:
- https://hdl.handle.net/20.500.12585/12586
https://dx.doi.org/10.18687/LACCEI2023.1.1.955
- Palabra clave:
- Dental caries
Oral microbiology
Antibiogram test
Mass spectrophotometry
- Rights
- openAccess
- License
- http://purl.org/coar/access_right/c_abf2
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Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries |
| title |
Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries |
| spellingShingle |
Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries Dental caries Oral microbiology Antibiogram test Mass spectrophotometry |
| title_short |
Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries |
| title_full |
Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries |
| title_fullStr |
Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries |
| title_full_unstemmed |
Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries |
| title_sort |
Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries |
| dc.creator.fl_str_mv |
Jimenez-Ramirez, Verónica Alejandra Chavarro Mesa, Edisson Acevedo Barrios, Rosa Leonor Ariza Rúa, Danilo Lusbin Martínez Santos, Juan Carlos |
| dc.contributor.author.none.fl_str_mv |
Jimenez-Ramirez, Verónica Alejandra Chavarro Mesa, Edisson Acevedo Barrios, Rosa Leonor Ariza Rúa, Danilo Lusbin Martínez Santos, Juan Carlos |
| dc.contributor.other.none.fl_str_mv |
Parra-Giraldo, Claudia Marcela Sánchez-Quitian, Zilpa Adriana |
| dc.subject.keywords.spa.fl_str_mv |
Dental caries Oral microbiology Antibiogram test Mass spectrophotometry |
| topic |
Dental caries Oral microbiology Antibiogram test Mass spectrophotometry |
| description |
It is essential to understand bacterial succession during the development of dental caries and determine the microorganisms related to this disease. This work aimed to identify bacteria associated with cariogenic pieces and carry out an antibiogram assay. First, we isolated bacterial isolates using MALDI TOF-MS and species-specific PCR techniques. Next, we implemented an antibiogram test to evaluate antimicrobial susceptibility events in Streptococcus oralis, Staphylococcus epidermidis, and Bacillus cereus. Our results showed that the bacterial species frequently associated with cariogenic processes was S. oralis. The antibiogram analysis determined that the most effective antibiotic was streptomycin, with a minimal significant difference of 0.52. Furthermore, the phylogenetic reconstruction allowed confirming the identity of the Colombian isolates that were sequenced, corresponding to the species S. oralis. Finally, we describe the complex etiology of dental caries where not only one bacterial agent is related to the development of this pathology. It is a complex of species. |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-12-11T12:21:34Z |
| dc.date.available.none.fl_str_mv |
2023-12-11T12:21:34Z |
| dc.date.issued.none.fl_str_mv |
2023-12-09 |
| dc.date.submitted.none.fl_str_mv |
2023-12-09 |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_c94f |
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http://purl.org/coar/resource_type/c_c94f |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Jimenez-Ramirez, V. A., Sánchez-Quitian, Z. A., Acevedo-Barrios, R., Ariza-Rua, D. L., Martinez-Santos, J. C., Parra-Giraldo, C. M., & Chavarro-Mesa, E. Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries. |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12585/12586 |
| dc.identifier.doi.none.fl_str_mv |
https://dx.doi.org/10.18687/LACCEI2023.1.1.955 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Tecnológica de Bolívar |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Universidad Tecnológica de Bolívar |
| identifier_str_mv |
Jimenez-Ramirez, V. A., Sánchez-Quitian, Z. A., Acevedo-Barrios, R., Ariza-Rua, D. L., Martinez-Santos, J. C., Parra-Giraldo, C. M., & Chavarro-Mesa, E. Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries. Universidad Tecnológica de Bolívar Repositorio Universidad Tecnológica de Bolívar |
| url |
https://hdl.handle.net/20.500.12585/12586 https://dx.doi.org/10.18687/LACCEI2023.1.1.955 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Cartagena de Indias |
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Ingeniería de Sistemas y Computación |
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Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology |
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Universidad Tecnológica de Bolívar |
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Jimenez-Ramirez, Verónica Alejandra195e2529-c0f3-44e9-a4aa-09bd2020644fChavarro Mesa, Edissonvirtual::693-1Acevedo Barrios, Rosa Leonorvirtual::759-1Ariza Rúa, Danilo Lusbinvirtual::3216-1Martínez Santos, Juan Carlosvirtual::3217-1Parra-Giraldo, Claudia MarcelaSánchez-Quitian, Zilpa Adriana2023-12-11T12:21:34Z2023-12-11T12:21:34Z2023-12-092023-12-09Jimenez-Ramirez, V. A., Sánchez-Quitian, Z. A., Acevedo-Barrios, R., Ariza-Rua, D. L., Martinez-Santos, J. C., Parra-Giraldo, C. M., & Chavarro-Mesa, E. Identification by MALDI-TOF MS of bacterial microorganisms associated with dental caries.https://hdl.handle.net/20.500.12585/12586https://dx.doi.org/10.18687/LACCEI2023.1.1.955Universidad Tecnológica de BolívarRepositorio Universidad Tecnológica de BolívarIt is essential to understand bacterial succession during the development of dental caries and determine the microorganisms related to this disease. This work aimed to identify bacteria associated with cariogenic pieces and carry out an antibiogram assay. First, we isolated bacterial isolates using MALDI TOF-MS and species-specific PCR techniques. Next, we implemented an antibiogram test to evaluate antimicrobial susceptibility events in Streptococcus oralis, Staphylococcus epidermidis, and Bacillus cereus. Our results showed that the bacterial species frequently associated with cariogenic processes was S. oralis. The antibiogram analysis determined that the most effective antibiotic was streptomycin, with a minimal significant difference of 0.52. Furthermore, the phylogenetic reconstruction allowed confirming the identity of the Colombian isolates that were sequenced, corresponding to the species S. oralis. Finally, we describe the complex etiology of dental caries where not only one bacterial agent is related to the development of this pathology. It is a complex of species.application/pdfengProceedings of the LACCEI international Multi-conference for Engineering, Education and TechnologyIdentification by MALDI-TOF MS of bacterial microorganisms associated with dental cariesArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_c94fhttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Dental cariesOral microbiologyAntibiogram testMass spectrophotometryinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Cartagena de IndiasIngeniería de Sistemas y ComputaciónPúblico generalX. Cerón-Bastidas, “El sistema ICDAS como método complementario para el diagnóstico de caries dental,” CES odontologia, vol. 28, no. 2. pp. 100–109, 2015.S. Suárez, C. Basantes-Acurio, J. Alvarez-Lopéz, “Microbiologia de las placas bacterianas dentales.” 2013.M. Negroni, “Microbiologia Estomatologica.” Panamericana, Buenos Aires, 2009. [Online]. Available: https://books.google.com.co/books?id=Gxmui vjZBgC&printsec=frontcover&hl=es&source=gbs_ge _summary_r&cad=0#v=onepage&q&f=falseS. K. Filoche, S. A. Anderson, C. H. Sissons, “Biofilm growth of Lactobacillus species is promoted by Actinomyces species and Streptococcus mutans,” Oral Microbiol. Immunol., vol. 19, no. 5, pp. 322–326, 2004.J. Liébana Ureña, “Microbiología oral,” 2002.M. R. Becker et al., “Molecular analysis of bacterial species associated with childhood caries,” J. Clin. Microbiol., vol. 40, no. 3, pp. 1001–1009, 2002.R. Palencia Díaz, “Farmacologia y terapéutica aplicada a la odontologia.” Buenos Aires, 2016.E. Santambrosio, M. Ortega, P. A. Garibaldi, “Preparacion de medios de cultivo.” 2009. [Online]. Available: https://www.frro.utn.edu.ar/repositorio/catedras/quimi ca/5_anio/biotecnologia/practicoI.pdfL. Sánchez, E. Sáenz, “Antisépticos y desinfectantes,” Dermatología Peru., vol. 15, no. 2, pp. 89–90, 2005.R. Sacsaquispe Contreras, J. Velásquez Pomar, “Manual de procedimientos para la prueba de sensibilidad antimicrobiana por el método de disco difusión,” 2008M. Fennix-Agudelo et al., “MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band.” LACCEI, 2022.J. Pol et al., “Trial Watch—Oncolytic viruses and cancer therapy,” Oncoimmunology, vol. 5, no. 2, p. e1117740, 2016K. Oliwa-Stasiak, C. I. Molnar, K. Arshak, M. Bartoszcze, C. C. Adley, “Development of a PCR assay for identification of the Bacillus cereus group species,” J. Appl. Microbiol., 2010, doi: 10.1111/j.1365- 2672.2009.04419.x.T. G. da Silva Paim, K. C. Reiter, C. de Oliveira d’Azevedo, “MALDI-TOF MS performance to identify gram-positive cocci clinical isolates in Porto Alegre,” Brazil J. Infect. Control, vol. 2, pp. 112–116, 2013.H. A. Serrano-Coll, M. Sánchez-Jiménez, N. Cardona Castro, “Conocimiento de la microbiota de la cavidad oral a través de la metagenómica,” CES Odontol., vol. 28, no. 2, pp. 112–118, 2015.J. Deglovic, N. Majtanova, J. Majtan, “Antibacterial and Antibiofilm Effect of Honey in the Prevention of Dental Caries: A Recent Perspective,” Foods, vol. 11, no. 17, p. 2670, 2022W. Qiu et al., “Application of antibiotics/antimicrobial agents on dental caries,” Biomed Res. Int., vol. 2020, 2020.M. 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