Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes
Stem canker and black scurf are potato diseases caused by the fungus Rhizoctonia solani, anastomosisgroup 3 (AG-3PT). This fungus affects potato roots, stems, and tubers, reducing crop yields. This study aimsto determine the genetic diversity of R. solaniAG-3PT obtained from the Colombian department...
- Autores:
-
Chavarro Mesa, Edisson
Herrera-Blanco, Néstor Andrés
Beltrán-Acosta, Camilo Rubén
Cotes-Prado, Alba Marina
Ángel-Díaz, Jorge Evelio
- Tipo de recurso:
- Fecha de publicación:
- 2021
- Institución:
- Universidad Tecnológica de Bolívar
- Repositorio:
- Repositorio Institucional UTB
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.utb.edu.co:20.500.12585/10632
- Acceso en línea:
- https://hdl.handle.net/20.500.12585/10632
https://doi.org/10.21930/rcta.vol22_num3_art:1888
- Palabra clave:
- Random amplified microsatellites (RAMs)
Ribosomal DNA
Solanum phureja
Solanum tuberosum
Thanatephorus cucumeris
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
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| dc.title.spa.fl_str_mv |
Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes |
| title |
Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes |
| spellingShingle |
Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes Random amplified microsatellites (RAMs) Ribosomal DNA Solanum phureja Solanum tuberosum Thanatephorus cucumeris |
| title_short |
Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes |
| title_full |
Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes |
| title_fullStr |
Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes |
| title_full_unstemmed |
Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes |
| title_sort |
Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes |
| dc.creator.fl_str_mv |
Chavarro Mesa, Edisson Herrera-Blanco, Néstor Andrés Beltrán-Acosta, Camilo Rubén Cotes-Prado, Alba Marina Ángel-Díaz, Jorge Evelio |
| dc.contributor.author.none.fl_str_mv |
Chavarro Mesa, Edisson Herrera-Blanco, Néstor Andrés Beltrán-Acosta, Camilo Rubén Cotes-Prado, Alba Marina Ángel-Díaz, Jorge Evelio |
| dc.subject.keywords.spa.fl_str_mv |
Random amplified microsatellites (RAMs) Ribosomal DNA Solanum phureja Solanum tuberosum Thanatephorus cucumeris |
| topic |
Random amplified microsatellites (RAMs) Ribosomal DNA Solanum phureja Solanum tuberosum Thanatephorus cucumeris |
| description |
Stem canker and black scurf are potato diseases caused by the fungus Rhizoctonia solani, anastomosisgroup 3 (AG-3PT). This fungus affects potato roots, stems, and tubers, reducing crop yields. This study aimsto determine the genetic diversity of R. solaniAG-3PT obtained from the Colombian departments of Antioquia, Boyacá, and Cundinamarca. Restrictionfragment lengthpolymorphism(RFLP) analysis of the ITS-5.8S ribosomal DNA region allowed the differentiation and specific identification of AG-3PT and AG2-1 anastomosis groups. These results confirm that AG-3PT is the primary causative agent and etiologicalorigin of stem canker and black scurf in Colombia. Two distinct subgroups within R. solaniAG-3PT were identified using random amplified microsatellite markers (RAMs); AG-3PT (A) shares a similarity index of 78%, and AG-3PT (B) shares a similarity of 79% among its isolates. These subgroups were not linked to a geographical origin but to the AG group to which they belong. Nei’s genetic diversity [D] of 0.25 confirmed the high genetic diversity for AG-3PT through RAMsanalysis, related to a high evolutive potential within the AG-3PT group in Colombia. Finally, the R. solaniAG-3PT fungus obtained from Cundinamarca had the adaptative potential to emerge as a pathogen of Solanum phurejain Colombia, possibly due tothe similarity between pathosystems. |
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2021 |
| dc.date.issued.none.fl_str_mv |
2021-10-18 |
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2022-03-18T18:46:30Z |
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2022-03-18T18:46:30Z |
| dc.date.submitted.none.fl_str_mv |
2022-03-18 |
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info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Chavarro-Mesa, E., Herrera-Blanco, N. E., Beltrán-Acosta, C. R., Cotes-Prado, A. M., & Ángel-Díaz, J. E. (2021). Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes. Ciencia y TecnologíaAgropecuaria, 22(3), e1888. https://doi.org/10.21930/rcta.vol22_num3_art:1888 |
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https://hdl.handle.net/20.500.12585/10632 |
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https://doi.org/10.21930/rcta.vol22_num3_art:1888 |
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Universidad Tecnológica de Bolívar |
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Repositorio Universidad Tecnológica de Bolívar |
| identifier_str_mv |
Chavarro-Mesa, E., Herrera-Blanco, N. E., Beltrán-Acosta, C. R., Cotes-Prado, A. M., & Ángel-Díaz, J. E. (2021). Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes. Ciencia y TecnologíaAgropecuaria, 22(3), e1888. https://doi.org/10.21930/rcta.vol22_num3_art:1888 Universidad Tecnológica de Bolívar Repositorio Universidad Tecnológica de Bolívar |
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https://hdl.handle.net/20.500.12585/10632 https://doi.org/10.21930/rcta.vol22_num3_art:1888 |
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eng |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
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24 Páginas |
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Chavarro Mesa, Edisson800ba77d-129a-48f9-bc50-2a8c25be1208Herrera-Blanco, Néstor Andrés311ee86a-fa90-4d93-b70a-9f0c08b94277Beltrán-Acosta, Camilo Rubén1f6463b8-c8a7-4074-b6de-3b3823154eb8Cotes-Prado, Alba Marina59abbf12-95ef-4b9e-a703-89cb15ef5bd4Ángel-Díaz, Jorge Evelio1ddbfb9b-542f-4e38-99b4-3cb2c41a1d132022-03-18T18:46:30Z2022-03-18T18:46:30Z2021-10-182022-03-18Chavarro-Mesa, E., Herrera-Blanco, N. E., Beltrán-Acosta, C. R., Cotes-Prado, A. M., & Ángel-Díaz, J. E. (2021). Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoes. Ciencia y TecnologíaAgropecuaria, 22(3), e1888. https://doi.org/10.21930/rcta.vol22_num3_art:1888https://hdl.handle.net/20.500.12585/10632https://doi.org/10.21930/rcta.vol22_num3_art:1888Universidad Tecnológica de BolívarRepositorio Universidad Tecnológica de BolívarStem canker and black scurf are potato diseases caused by the fungus Rhizoctonia solani, anastomosisgroup 3 (AG-3PT). This fungus affects potato roots, stems, and tubers, reducing crop yields. This study aimsto determine the genetic diversity of R. solaniAG-3PT obtained from the Colombian departments of Antioquia, Boyacá, and Cundinamarca. Restrictionfragment lengthpolymorphism(RFLP) analysis of the ITS-5.8S ribosomal DNA region allowed the differentiation and specific identification of AG-3PT and AG2-1 anastomosis groups. These results confirm that AG-3PT is the primary causative agent and etiologicalorigin of stem canker and black scurf in Colombia. Two distinct subgroups within R. solaniAG-3PT were identified using random amplified microsatellite markers (RAMs); AG-3PT (A) shares a similarity index of 78%, and AG-3PT (B) shares a similarity of 79% among its isolates. These subgroups were not linked to a geographical origin but to the AG group to which they belong. Nei’s genetic diversity [D] of 0.25 confirmed the high genetic diversity for AG-3PT through RAMsanalysis, related to a high evolutive potential within the AG-3PT group in Colombia. Finally, the R. solaniAG-3PT fungus obtained from Cundinamarca had the adaptative potential to emerge as a pathogen of Solanum phurejain Colombia, possibly due tothe similarity between pathosystems.24 Páginasapplication/pdfenghttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://purl.org/coar/access_right/c_abf2Cienc. Tecnol. Agropecuaria, 22(3): e1888Genetic diversity of Rhizoctonia solaniAG-3PT, the etiological cause of stem canker and black scurf in Colombian potatoesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/restrictedAccesshttp://purl.org/coar/resource_type/c_2df8fbb1Random amplified microsatellites (RAMs)Ribosomal DNASolanum phurejaSolanum tuberosumThanatephorus cucumerisCartagena de IndiasInvestigadoresAjayi-Oyetunde, O. O., & Bradley, C. A. (2017). Identification and Characterization of Rhizoctonia Species Associated with Soybean Seedling Disease. Plant Disease, 101(4), 520-533.Aliferis, K. A., & Jabaji, S. (2012). FT-ICR/MS and GC-EI/MS metabolomics networking unravels global potato sprout’s responses to Rhizoctonia solani infection. PLoS ONE, 7(8).Anderson, N. A. (1982). The Genetics and Pathology of Rhizoctonia Solani. Annual Review of Phytopathology, 20, 329-347Anguiz, R., & Martin, C. (1989). Anastomosis groups, pathogenicity, and other characteristics of Rhizoctonia solani isolated from potatoes in Peru. Plant Disease, 73(3), 199-201.Balali, G. R., Whisson, D. L., Scott, E. S., & Neate, S. M. (1996). DNA fingerprinting probe specific to isolates of Rhizoctonia solani AG-3. Mycological Research, 100(4), 467-470.Horsfall, J. G., & Barratt, R. W. (1945). An improved grading system for measuring plant disease. Phytopathology, 35, 655.Beltrán, C., Cotes, A. M., & Becerra, A. P. (2007). Selection of isolates of Trichoderma spp. with biocontrol activity over Rhizoctonia solani in potato. IOBC WPRS Bulletin, 30(6/2), 55-58.Beltrán, C., Velandia, C., & Cotes, A. (2011). Trichoderma koningiopsis Th003, a biological alternative in the control of Rhizoctonia solani in potato cultivars. CORPOICA.Campion, C., Chatot, C., Perraton, B., & Andrivon, D. (2003). Anastomosis groups, pathogenicity and sensitivity to fungicides of Rhizoctonia solani isolates collected on potato crops in France. European Journal of Plant Pathology, 109(9), 983-992.Carling, D. E., Brainard, K. A., Virgen-Calleros, G., & Olalde-Portugal, V. (1998). First Report of Rhizoctonia solani AG-7 on Potato in Mexico. Plant Disease, 82(1), 127.Carling, D. E., Kuninaga, S., & Brainard, K. A. (2002). Hyphal anastomosis reactions, rDNA-internal transcribed spacer sequences, and virulence levels among subsets of Rhizoctonia solani anastomosis group-2 (AG-2) and AG-BI. Phytopathology, 92(1), 43-50.Cedeño, L., Carrero, C., Quintero, K., Araujo, Y., Pino, H., & García, R. (2001). Identificación y virulencia de grupos de anastomosis de Rhizoctonia solani Kühn asociados con papa en Mérida, Venezuela. Interciencia, 26(7), 296-300.Ceresini, P. C., Shew, H. D., & Cubeta, M. A. (1999). RFLP analysis of the PCR amplified ribosomal DNA regions ITS and IGS indicated that isolates of Rhizoctonia solani from potato and tobacco represent distinct groups within the anastomosis group 3. Phytopathology, 89, S12.Ceresini, P. C., Shew, H. D., Vilgalys, R. J., & Cubeta, M. A. (2002). Genetic diversity of Rhizoctonia solani AG-3 from potato and tobacco in North Carolina. Mycologia, 94(3), 437-449Ceresini, P. C., Shew, H. D., Vilgalys, R. J., Rosewich, U. L., & Cubeta, M. A. (2002). Genetic structure of populations of Rhizoctonia solani AG-3 on potato in eastern North Carolina. Mycologia, 94(3), 450-460.Chavarro-Mesa, E., Ceresini, P. C., Ramos Molina, L. M., Pereira, D. A. S., Schurt, D. A., Vieira, J. R., & McDonald, B. A. (2015). The Urochloa foliar blight and collar rot pathogen Rhizoctonia solani AG-1 IA emerged in South America via a host shift from rice. 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(2002). rDNA-ITS nucleotide sequences analysis of Thanatephorus cucumeris AG-1 associated with web blight on common beans in Central America and Caribbean. Japanese Journal of Phytopathology, 68, 3-20.Lees, A. K., Cullen, D. W., Sullivan, L., & Nicolson, M. J. (2002). Development of conventional and quantitative real-time PCR assays for the detection and identification of Rhizoctonia solani AG-3 in potato and soil. Plant Pathology, 51(3), 293-302.Mandujano, M. C., Montaña, C., Franco, M., Golubov, J., & Flores-Martínez, A. (2001). Integration of demographic annual variability in a clonal desert cactus. Ecology, 82(2), 344-359.Mandujano, M. del C., Montaña, C., Méndez, I., & Golubov, J. (1998). The relative contributions of sexual reproduction and clonal propagation in Opuntia rastrera from two habitats in the Chihuahuan Desert. Journal of Ecology, 86(6), 911-921.McDonald, B. A. (1997). The population genetics of fungi: tools and techniques. Phytopathology, 87(4), 448-453.McDonald, B. A., & Linde, C. (2002). Pathogen population genetics, evolutionary potential, and durable resistance. Annual Review of Phytopathology, 40(1), 349-379.Mogie, M., & Hutchings, M. (1990). Phylogeny, ontogeny and clonal growth in vascular plants. In J. van Groenendael & H. de Kroon (Eds.), Clonal Growth in Plants: Regulation and Function (pp. 3-22). SPB Academic Publishing. Muzhinji, N., Truter, M., Woodhall, J. W., & Van der Waals, J. E. (2015). Anastomosis groups and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia from potato in South Africa. Plant Disease, 99(12), 1790-1802.Muzhinji, N., Woodhall, J. W., Truter, M., & Van der Waals, J. E. (2017). Relative contribution of seed tuber- and soilborne inoculum to potato disease development and changes in the population genetic structure of Rhizoctonia solani AG 3-PT under Field Conditions in South Africa. Plant Disease, 102(1), 60-66.Nei, M. (1973). Analysis of Gene Diversity in Subdivided Populations. 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