Bioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia

Three extremophile bacterial strains (BBCOL-009, BBCOL-014 and BBCOL-015), capable of degrading high concentrations of perchlorate at a range of pH (6.5 to 10.0), were isolated from Colombian Caribbean Coast sediments. Morphological features included Gram negative strain bacilli with sizes averaged...

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Autores:
Acevedo-Barrios, Rosa
Tirado‑Ballestas, Irina
Bertel‑Sevilla, Angela
Cervantes‑Ceballos, Leonor
Gallego, Jorge L.
Leal, María Angélica
Tovar, David
Olivero‑Verbel, Jesús
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Tecnológica de Bolívar
Repositorio:
Repositorio Institucional UTB
Idioma:
eng
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oai:repositorio.utb.edu.co:20.500.12585/12666
Acceso en línea:
https://hdl.handle.net/20.500.12585/12666
Palabra clave:
Bioremediation
Halophytes
Marine sediment
RNA 16S
Scopus
LEMB
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openAccess
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http://creativecommons.org/licenses/by-nc-nd/4.0/
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network_name_str Repositorio Institucional UTB
repository_id_str
dc.title.spa.fl_str_mv Bioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia
title Bioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia
spellingShingle Bioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia
Bioremediation
Halophytes
Marine sediment
RNA 16S
Scopus
LEMB
title_short Bioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia
title_full Bioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia
title_fullStr Bioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia
title_full_unstemmed Bioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia
title_sort Bioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia
dc.creator.fl_str_mv Acevedo-Barrios, Rosa
Tirado‑Ballestas, Irina
Bertel‑Sevilla, Angela
Cervantes‑Ceballos, Leonor
Gallego, Jorge L.
Leal, María Angélica
Tovar, David
Olivero‑Verbel, Jesús
dc.contributor.author.none.fl_str_mv Acevedo-Barrios, Rosa
Tirado‑Ballestas, Irina
Bertel‑Sevilla, Angela
Cervantes‑Ceballos, Leonor
Gallego, Jorge L.
Leal, María Angélica
Tovar, David
Olivero‑Verbel, Jesús
dc.subject.keywords.spa.fl_str_mv Bioremediation
Halophytes
Marine sediment
RNA 16S
topic Bioremediation
Halophytes
Marine sediment
RNA 16S
Scopus
LEMB
dc.subject.armarc.none.fl_str_mv Scopus
LEMB
description Three extremophile bacterial strains (BBCOL-009, BBCOL-014 and BBCOL-015), capable of degrading high concentrations of perchlorate at a range of pH (6.5 to 10.0), were isolated from Colombian Caribbean Coast sediments. Morphological features included Gram negative strain bacilli with sizes averaged of 1.75 × 0.95, 2.32 × 0.65 and3.08 × 0.70 μm, respectively. The reported strains tolerate a wide range of pH (6.5 to 10.0); concentrations of NaCl (3.5 to 7.5% w/v) and KClO4− (250 to 10000 mg/L), reduction of KClO4 − from 10 to 25%. LB broth with NaCl (3.5–30% w/v) and KClO4ˉ(250-10000 mg/L) were used in independent trialsto evaluate susceptibility to salinity and perchlorate,respectively. Isolates increased their biomass at 7.5 % (w/v) NaCl with optimal development at 3.5 % NaCl. Subsequently, ClO reduction was assessed using LB medium with 3.5% NaCl and 10000 mg/L ClO4 BBCOL-009, BBCOL-014 and BBCOL-015 achieved 10%, 17%, and 25% reduction of ClO4 respectively. The 16 S rRNA gene sequence grouped them as Bacillus flexus T6186-2, Bacillus marisflavi TF-11 (T), and Bacillus vietnamensis 15 − 1 (T) respectively, with < 97.5% homology. In addition, antimicrobial resistance to ertapenem, vancomycine, amoxicillin clavulanate, penicillin, and erythromycin was present in all the isolates, indicating their high adaptability to stressful environments. The isolated strains from marine sediments in Cartagena Bay, Colombia are suitable candidates to reduce perchlorate contamination in different environments. Although the primary focus of the study of perchlorate-reducing and resistant bacteria is in the ecological and agricultural realms, from an astrobiological perspective, perchlorate-resistant bacteria serve as models for astrobiological investigations.
publishDate 2023
dc.date.issued.none.fl_str_mv 2023-12-12
dc.date.accessioned.none.fl_str_mv 2024-04-22T16:49:51Z
dc.date.available.none.fl_str_mv 2024-04-22T16:49:51Z
dc.date.submitted.none.fl_str_mv 2024-04-22
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dc.identifier.citation.spa.fl_str_mv Acevedo-Barrios, R., Tirado-Ballestas, I., Bertel-Sevilla, A. et al. Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia. Biodegradation (2024). https://doi.org/10.1007/s10532-024-10079-0
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12585/12666
dc.identifier.doi.none.fl_str_mv 10.1007/s10532-024-10079-0
dc.identifier.instname.spa.fl_str_mv Universidad Tecnológica de Bolívar
dc.identifier.reponame.spa.fl_str_mv Repositorio Universidad Tecnológica de Bolívar
identifier_str_mv Acevedo-Barrios, R., Tirado-Ballestas, I., Bertel-Sevilla, A. et al. Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia. Biodegradation (2024). https://doi.org/10.1007/s10532-024-10079-0
10.1007/s10532-024-10079-0
Universidad Tecnológica de Bolívar
Repositorio Universidad Tecnológica de Bolívar
url https://hdl.handle.net/20.500.12585/12666
dc.language.iso.spa.fl_str_mv eng
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dc.coverage.spatial.none.fl_str_mv Colombia, Bolívar, Cartagena
dc.publisher.place.spa.fl_str_mv Cartagena de Indias
dc.publisher.sede.spa.fl_str_mv Campus Tecnológico
dc.publisher.discipline.spa.fl_str_mv Ingeniería Ambiental
dc.source.spa.fl_str_mv Biodegradation
institution Universidad Tecnológica de Bolívar
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spelling Acevedo-Barrios, Rosabcae3c53-60bb-47b1-bf6b-2a314645eb75Tirado‑Ballestas, Irinaa64c07a4-58a6-4d8b-86f8-0230ba696d16Bertel‑Sevilla, Angela3f5b1994-4d28-43b5-9881-3dfa2491bb0bCervantes‑Ceballos, Leonor98a5dde8-a21b-4b8d-b2d1-83736201a813Gallego, Jorge L.1a4d56d1-cf5b-4a37-bf7f-063c6c452b4dLeal, María Angélicaffea7f08-0512-42e9-ba95-d2f54f6dc19bTovar, Davidc16d4a5b-15da-45f1-917d-258a97e1a7e2Olivero‑Verbel, Jesús13f634ce-4c2a-44b0-90ab-6bcfb029c715Colombia, Bolívar, Cartagena2024-04-22T16:49:51Z2024-04-22T16:49:51Z2023-12-122024-04-22Acevedo-Barrios, R., Tirado-Ballestas, I., Bertel-Sevilla, A. et al. Bioprospecting of extremophilic perchlorate-reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombia. Biodegradation (2024). https://doi.org/10.1007/s10532-024-10079-0https://hdl.handle.net/20.500.12585/1266610.1007/s10532-024-10079-0Universidad Tecnológica de BolívarRepositorio Universidad Tecnológica de BolívarThree extremophile bacterial strains (BBCOL-009, BBCOL-014 and BBCOL-015), capable of degrading high concentrations of perchlorate at a range of pH (6.5 to 10.0), were isolated from Colombian Caribbean Coast sediments. Morphological features included Gram negative strain bacilli with sizes averaged of 1.75 × 0.95, 2.32 × 0.65 and3.08 × 0.70 μm, respectively. The reported strains tolerate a wide range of pH (6.5 to 10.0); concentrations of NaCl (3.5 to 7.5% w/v) and KClO4− (250 to 10000 mg/L), reduction of KClO4 − from 10 to 25%. LB broth with NaCl (3.5–30% w/v) and KClO4ˉ(250-10000 mg/L) were used in independent trialsto evaluate susceptibility to salinity and perchlorate,respectively. Isolates increased their biomass at 7.5 % (w/v) NaCl with optimal development at 3.5 % NaCl. Subsequently, ClO reduction was assessed using LB medium with 3.5% NaCl and 10000 mg/L ClO4 BBCOL-009, BBCOL-014 and BBCOL-015 achieved 10%, 17%, and 25% reduction of ClO4 respectively. The 16 S rRNA gene sequence grouped them as Bacillus flexus T6186-2, Bacillus marisflavi TF-11 (T), and Bacillus vietnamensis 15 − 1 (T) respectively, with < 97.5% homology. In addition, antimicrobial resistance to ertapenem, vancomycine, amoxicillin clavulanate, penicillin, and erythromycin was present in all the isolates, indicating their high adaptability to stressful environments. The isolated strains from marine sediments in Cartagena Bay, Colombia are suitable candidates to reduce perchlorate contamination in different environments. Although the primary focus of the study of perchlorate-reducing and resistant bacteria is in the ecological and agricultural realms, from an astrobiological perspective, perchlorate-resistant bacteria serve as models for astrobiological investigations.Universidad Tecnológica de Bolívar20 páginasapplication/pdfenghttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://purl.org/coar/access_right/c_abf2BiodegradationBioprospecting of extremophilic perchlorate‑reducing bacteria: report of promising Bacillus spp. isolated from sediments of the bay of Cartagena, Colombiainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1BioremediationHalophytesMarine sedimentRNA 16SScopusLEMBCartagena de IndiasCampus TecnológicoIngeniería AmbientalPúblico generalAcevedo Barrios RL, Hernández Rocha I, Puentes Martinez D et al (2023) Psychrobacter sp: perchlorate reducing bacteria, isolated from marine sediments from Margarita Bay, Antarctica. In: Proceedings of the 21th LACCEI International Multi-Conference for Engineering, and Technology (LACCEI 2023): Leadership in Education and Innovation in Engineering in the Framework of Global Transformations: Integration and Alliances for Integral Development. Latin American and Caribbean Consortium of Engineering Institutions. https:// doi. org/ 10. 18687/ LACCE I2023.1. 1. 995Acevedo-Barrios R, Olivero-Verbel J (2021) Perchlorate contamination: sources, effects, and technologies for Remediation. In: de Voogt P (ed) Reviews of environmental contamination and toxicology volume 256. Springer International Publishing, Cham, pp 103–120Acevedo-Barrios R, Bertel-Sevilla A, Alonso-Molina J, Olivero- Verbel J (2016) Perchlorate tolerant bacteria from saline environments at the Caribbean region of Colombia. Toxicol Lett 259:S103. https:// doi. org/ 10. 1016/J. 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