MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease
White Band Disease (WBD) is one of the most harmful coral syndromes in the Caribbean, deteriorating the structure of many corals during the eighties and nineties. Since its etiology is not entirely known, the use of techniques aimed a t identifying the associated agents could provide an approach to...
- Autores:
-
Fennix Agudelo, Mary Andrea
Zarza González, Esteban
Sánchez Quitian, Zilpa Adriana
Castellanos, Nuri
Martínez Santos, Juan Carlos
Parra Giraldo, Claudia Marcela
Chavarro Mesa, Edisson
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Tecnológica de Bolívar
- Repositorio:
- Repositorio Institucional UTB
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.utb.edu.co:20.500.12585/12380
- Acceso en línea:
- https://hdl.handle.net/20.500.12585/12380
- Palabra clave:
- Anthozoa;
Acropora;
Microorganisms
LEMB
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
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MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease |
| title |
MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease |
| spellingShingle |
MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease Anthozoa; Acropora; Microorganisms LEMB |
| title_short |
MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease |
| title_full |
MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease |
| title_fullStr |
MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease |
| title_full_unstemmed |
MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease |
| title_sort |
MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease |
| dc.creator.fl_str_mv |
Fennix Agudelo, Mary Andrea Zarza González, Esteban Sánchez Quitian, Zilpa Adriana Castellanos, Nuri Martínez Santos, Juan Carlos Parra Giraldo, Claudia Marcela Chavarro Mesa, Edisson |
| dc.contributor.author.none.fl_str_mv |
Fennix Agudelo, Mary Andrea Zarza González, Esteban Sánchez Quitian, Zilpa Adriana Castellanos, Nuri Martínez Santos, Juan Carlos Parra Giraldo, Claudia Marcela Chavarro Mesa, Edisson |
| dc.subject.keywords.spa.fl_str_mv |
Anthozoa; Acropora; Microorganisms |
| topic |
Anthozoa; Acropora; Microorganisms LEMB |
| dc.subject.armarc.none.fl_str_mv |
LEMB |
| description |
White Band Disease (WBD) is one of the most harmful coral syndromes in the Caribbean, deteriorating the structure of many corals during the eighties and nineties. Since its etiology is not entirely known, the use of techniques aimed a t identifying the associated agents could provide an approach to the composition of the populations involved in the deterioration of A. cervicornis. Therefore, this research's objective was to isolate and characterize bacteria associated with WBD in A. cervicornis from hanging nurseries of Rosario and San Bernardo Corals National Natural Park, Colombia, using MALDI-TO F MS and PCR. The results showed the presence of Bacillus cereus bacteria in individuals with bleaching signs from nurseries and transplant areas on Isla Tesoro and Isla Ceiner. One of these strains sequenced and phylogenetically analyzed was closely related t o Bacillus thuringiensis, with similarities of up to 99%. The presence of B. cereus could indicate a special relationship between this microorganism and WBD. However, it is imperative to carry out more studies on identifying the bacteria associated with this disease to support these findings. © 2022 Latin American and Caribbean Consortium of Engineering Institutions. All rights reserved. |
| publishDate |
2022 |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2023-07-21T20:48:29Z |
| dc.date.available.none.fl_str_mv |
2023-07-21T20:48:29Z |
| dc.date.submitted.none.fl_str_mv |
2023 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/draft |
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http://purl.org/coar/resource_type/c_6501 |
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draft |
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Fennix-Agudelo, M. A., Zarza-González, E., Sánchez-Quitian, Z. A., Merchán-Castellanos, N., Martinez-Santos, J. C., Parra-Giraldo, C. M., & Chavarro-Mesa, E. MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band. |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12585/12380 |
| dc.identifier.doi.none.fl_str_mv |
10.18687/LACCEI2022.1.1.714 |
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Universidad Tecnológica de Bolívar |
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Repositorio Universidad Tecnológica de Bolívar |
| identifier_str_mv |
Fennix-Agudelo, M. A., Zarza-González, E., Sánchez-Quitian, Z. A., Merchán-Castellanos, N., Martinez-Santos, J. C., Parra-Giraldo, C. M., & Chavarro-Mesa, E. MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band. 10.18687/LACCEI2022.1.1.714 Universidad Tecnológica de Bolívar Repositorio Universidad Tecnológica de Bolívar |
| url |
https://hdl.handle.net/20.500.12585/12380 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
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openAccess |
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7 páginas |
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Cartagena de Indias |
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Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology |
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Fennix Agudelo, Mary Andrea1588a0ca-009d-4aca-8575-f9ce95f54615Zarza González, Esteban3e1d7a4b-51f9-4603-b44a-850b1d95d1e9Sánchez Quitian, Zilpa Adrianac6b988d9-38fb-4e79-9979-0a949652f1a0Castellanos, Nuric039f767-ebbe-4c94-97f8-c708ea1ff549Martínez Santos, Juan Carlos480cc438-b02b-45d2-bfc9-7bfa96f0271bParra Giraldo, Claudia Marcela70133c96-c400-49f2-9a70-df59fee3c0c7Chavarro Mesa, Edisson800ba77d-129a-48f9-bc50-2a8c25be12082023-07-21T20:48:29Z2023-07-21T20:48:29Z20222023Fennix-Agudelo, M. A., Zarza-González, E., Sánchez-Quitian, Z. A., Merchán-Castellanos, N., Martinez-Santos, J. C., Parra-Giraldo, C. M., & Chavarro-Mesa, E. MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band.https://hdl.handle.net/20.500.12585/1238010.18687/LACCEI2022.1.1.714Universidad Tecnológica de BolívarRepositorio Universidad Tecnológica de BolívarWhite Band Disease (WBD) is one of the most harmful coral syndromes in the Caribbean, deteriorating the structure of many corals during the eighties and nineties. Since its etiology is not entirely known, the use of techniques aimed a t identifying the associated agents could provide an approach to the composition of the populations involved in the deterioration of A. cervicornis. Therefore, this research's objective was to isolate and characterize bacteria associated with WBD in A. cervicornis from hanging nurseries of Rosario and San Bernardo Corals National Natural Park, Colombia, using MALDI-TO F MS and PCR. The results showed the presence of Bacillus cereus bacteria in individuals with bleaching signs from nurseries and transplant areas on Isla Tesoro and Isla Ceiner. One of these strains sequenced and phylogenetically analyzed was closely related t o Bacillus thuringiensis, with similarities of up to 99%. The presence of B. cereus could indicate a special relationship between this microorganism and WBD. However, it is imperative to carry out more studies on identifying the bacteria associated with this disease to support these findings. © 2022 Latin American and Caribbean Consortium of Engineering Institutions. All rights reserved.7 páginasapplication/pdfenghttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://purl.org/coar/access_right/c_abf2Proceedings of the LACCEI international Multi-conference for Engineering, Education and TechnologyMALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Diseaseinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/drafthttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/version/c_b1a7d7d4d402bccehttp://purl.org/coar/resource_type/c_2df8fbb1Anthozoa;Acropora;MicroorganismsLEMBCartagena de IndiasShinzato, C., Mungpakdee, S., Satoh, N., Shoguchi, E. A genomic approach to coral-dinoflagellate symbiosis: Studies of Acropora digitifera and Symbiodinium minutum (2014) Frontiers in Microbiology, 5 (JULY), art. no. 336. 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Cited 38 times. www.elsevier.com/inca/publications/store/6/0/0/3/1/8 doi: 10.1016/j.seares.2015.06.008Popović, N.T., Kazazić, S.P., Strunjak-Perović, I., Čož-Rakovac, R. Differentiation of environmental aquatic bacterial isolates by MALDI-TOF MS (Open Access) (2017) Environmental Research, 152, pp. 7-16. Cited 44 times. http://www.elsevier.com/inca/publications/store/6/2/2/8/2/1/index.htt doi: 10.1016/j.envres.2016.09.020Singhal, N., Kumar, M., Kanaujia, P.K., Virdi, J.S. MALDI-TOF mass spectrometry: An emerging technology for microbial identification and diagnosis (2015) Frontiers in Microbiology, 6 (AUG), art. no. 791. Cited 848 times. http://journal.frontiersin.org/article/10.3389/fmicb.2015.00791/full doi: 10.3389/fmicb.2015.00791Vidal, L.M.R., Venas, T.M., Gonçalves, A.R.P., Mattsson, H.K., Silva, R.V.P., Nóbrega, M.S., Azevedo, G.P.R., (...), Thompson, C.C. Rapid screening of marine bacterial symbionts using MALDI-TOF MS (2020) Archives of Microbiology, 202 (8), pp. 2329-2336. 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Cited 31 times. doi: 10.1111/j.1574-6941.2012.01319.xChelliah, R., Wei, S., Park, B.-J., Rubab, M., Banan-Mwine Dalirii, E., Barathikannan, K., Jin, Y.-G., (...), Oh, D.-H. Whole genome sequence of Bacillus thuringiensis ATCC 10792 and improved discrimination of Bacillus thuringiensis from Bacillus cereus group based on novel biomarkers (Open Access) (2019) Microbial Pathogenesis, 129, pp. 284-297. 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