Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life

In this study, active coatings based on carboxymethylcellulose (CMC) were prepared using liposomes filled with an aqueous extract of Melissa officinalis retained in high acyl gellan gum (HAG), low acyl gellan gum (LAG), and their mixture (HAG/LAG). The objective was to investigate the effect of thes...

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Autores:
González-Cuello, Rafael
Fuentes, Luis Gabriel
Castellanos, Heliana Milena
Hernández-Fernández, Joaquín
Ortega-Toro, Rodrigo
Tipo de recurso:
Fecha de publicación:
2024
Institución:
Universidad Tecnológica de Bolívar
Repositorio:
Repositorio Institucional UTB
Idioma:
eng
OAI Identifier:
oai:repositorio.utb.edu.co:20.500.12585/12708
Acceso en línea:
https://hdl.handle.net/20.500.12585/12708
Palabra clave:
Aqueous extract
Composite coatings
Gellan gum
Liposomes
Quality postharvest
Tomatoes
Rights
openAccess
License
http://creativecommons.org/publicdomain/zero/1.0/
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dc.title.spa.fl_str_mv Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life
title Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life
spellingShingle Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life
Aqueous extract
Composite coatings
Gellan gum
Liposomes
Quality postharvest
Tomatoes
title_short Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life
title_full Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life
title_fullStr Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life
title_full_unstemmed Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life
title_sort Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life
dc.creator.fl_str_mv González-Cuello, Rafael
Fuentes, Luis Gabriel
Castellanos, Heliana Milena
Hernández-Fernández, Joaquín
Ortega-Toro, Rodrigo
dc.contributor.author.none.fl_str_mv González-Cuello, Rafael
Fuentes, Luis Gabriel
Castellanos, Heliana Milena
Hernández-Fernández, Joaquín
Ortega-Toro, Rodrigo
dc.subject.keywords.spa.fl_str_mv Aqueous extract
Composite coatings
Gellan gum
Liposomes
Quality postharvest
Tomatoes
topic Aqueous extract
Composite coatings
Gellan gum
Liposomes
Quality postharvest
Tomatoes
description In this study, active coatings based on carboxymethylcellulose (CMC) were prepared using liposomes filled with an aqueous extract of Melissa officinalis retained in high acyl gellan gum (HAG), low acyl gellan gum (LAG), and their mixture (HAG/LAG). The objective was to investigate the effect of these coatings on postharvest preservation of tomato (Solanum lycopersicum) fruits. The tomato fruits were divided into four groups: (i) coating with HAG-based liposomes (WL-HAG), (ii) coating with LAG-based liposomes (WL-LAG), (iii) coating with HAG/LAG-based liposomes (WL-HAG/LAG), and (iv) control group treated with sterile water. Over a period of 10 days, various quality attributes, such as respiration rate, soluble solids, titratable acidity, luminosity, weight loss, malondialdehyde (MDA) content, hydrogen peroxide, total phenols, and DPPH scavenging ability, were studied. The results indicated that the WL-HAG coatings significantly (p < 0.05) decreased the respiration rate, hydrogen peroxide, and MDA content compared to the control fruits and other coatings. Therefore, WL-HAG could be considered a promising option to enhance postharvest preservation of tomato fruits in the Colombian fruit and vegetable industry.
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-08-14T12:15:14Z
dc.date.available.none.fl_str_mv 2024-08-14T12:15:14Z
dc.date.issued.none.fl_str_mv 2024-07-22
dc.date.submitted.none.fl_str_mv 2024-08-13
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dc.identifier.citation.spa.fl_str_mv González-Cuello, R.; Fuentes, L.G.; Castellanos, H.M.; Hernández-Fernández, J.; Ortega-Toro, R. Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life. J. Compos. Sci. 2024, 8, 283. https://doi.org/10.3390/jcs8070283
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12585/12708
dc.identifier.doi.none.fl_str_mv 10.3390/jcs8070283
dc.identifier.instname.spa.fl_str_mv Universidad Tecnológica de Bolívar
dc.identifier.reponame.spa.fl_str_mv Repositorio Universidad Tecnológica de Bolívar
identifier_str_mv González-Cuello, R.; Fuentes, L.G.; Castellanos, H.M.; Hernández-Fernández, J.; Ortega-Toro, R. Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life. J. Compos. Sci. 2024, 8, 283. https://doi.org/10.3390/jcs8070283
10.3390/jcs8070283
Universidad Tecnológica de Bolívar
Repositorio Universidad Tecnológica de Bolívar
url https://hdl.handle.net/20.500.12585/12708
dc.language.iso.spa.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
dc.format.extent.none.fl_str_mv 14 paginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.place.spa.fl_str_mv Cartagena de Indias
dc.publisher.sede.spa.fl_str_mv Campus Tecnológico
dc.source.spa.fl_str_mv Journal of Composites Sciences
institution Universidad Tecnológica de Bolívar
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spelling González-Cuello, Rafael095663a6-3ad0-4976-b75b-0805d4de1e3bFuentes, Luis Gabriel9be2b902-1332-458d-a35c-c873e0e29f89Castellanos, Heliana Milena698cd9c2-a44d-4e34-a2f2-b3985cae5e7bHernández-Fernández, Joaquínbc85d77e-b89b-40f6-a090-a475dc6dc160Ortega-Toro, Rodrigod594d4c1-6ec9-4782-a84b-cee2853ea3592024-08-14T12:15:14Z2024-08-14T12:15:14Z2024-07-222024-08-13González-Cuello, R.; Fuentes, L.G.; Castellanos, H.M.; Hernández-Fernández, J.; Ortega-Toro, R. Composite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Life. J. Compos. Sci. 2024, 8, 283. https://doi.org/10.3390/jcs8070283https://hdl.handle.net/20.500.12585/1270810.3390/jcs8070283Universidad Tecnológica de BolívarRepositorio Universidad Tecnológica de BolívarIn this study, active coatings based on carboxymethylcellulose (CMC) were prepared using liposomes filled with an aqueous extract of Melissa officinalis retained in high acyl gellan gum (HAG), low acyl gellan gum (LAG), and their mixture (HAG/LAG). The objective was to investigate the effect of these coatings on postharvest preservation of tomato (Solanum lycopersicum) fruits. The tomato fruits were divided into four groups: (i) coating with HAG-based liposomes (WL-HAG), (ii) coating with LAG-based liposomes (WL-LAG), (iii) coating with HAG/LAG-based liposomes (WL-HAG/LAG), and (iv) control group treated with sterile water. Over a period of 10 days, various quality attributes, such as respiration rate, soluble solids, titratable acidity, luminosity, weight loss, malondialdehyde (MDA) content, hydrogen peroxide, total phenols, and DPPH scavenging ability, were studied. The results indicated that the WL-HAG coatings significantly (p < 0.05) decreased the respiration rate, hydrogen peroxide, and MDA content compared to the control fruits and other coatings. Therefore, WL-HAG could be considered a promising option to enhance postharvest preservation of tomato fruits in the Colombian fruit and vegetable industry.14 paginasapplication/pdfenghttp://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccessCC0 1.0 Universalhttp://purl.org/coar/access_right/c_abf2Journal of Composites SciencesComposite Coatings with Liposomes of Melissa officinalis Extract for Extending Tomato Shelf Lifeinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Aqueous extractComposite coatingsGellan gumLiposomesQuality postharvestTomatoesCartagena de IndiasCampus TecnológicoInvestigadoresSalehi, B.; Sharifi-Rad, R.; Sharopov, F.; Namiesnik, J.; Roointan, A.; Kamle, M.; Kumar, P.M.; Martins, N.; Sharifi-Rad, J. Beneficial effects and potential risks of tomato consumption for human health: An overview. Nutrition 2019, 62, 201–208. [CrossRef] [PubMed]Baswal, A.K.; Dhaliwal, H.S.; Singh, Z.; Mahajan, B.; Kalia, A.; Gill, K. Influence of carboxy methylcellulose, chitosan and beeswax coatings on cold storage life and quality of Kinnow mandarin fruit. Sci. Hortic. 2020, 260, 108887.Ahmed, L.; Martin-Diana, A.B.; Rico, D.; Barry-Ryan, C. Effect of delactosed whey permeate treatment on physico-chemical, sensorial, nutritional and microbial properties of whole tomatoes during postharvest storage. LWT Food Sci. Technol. 2013, 51, 367–374.Ministerio de Agricultura y Desarrollo Rural de Colombia. 2019. Reporte: Área, Producción y Rendimiento Nacional por Cultivo (tomate) Agronet. Available online: https://www.agronet.gov.co/estadistica/Paginas/home.aspx?cod=1# (accessed on 25 July 2023).Mabrouki, H.; Duarte, C.M.; Akretche, D.E. Estimation of total phenolic contents and in vitro antioxidant and antimicrobial activities of various solvent extracts of Melissa officinalis L. Arab. J. Sci. Eng. 2018, 43, 3349–3357.Shakeri, A.; Khakdan, F.; Soheili, V.; Sahebkar, A.; Rassam, G.; Asili, J. Chemical composition, antibacterial activity, and cytotoxicity of essential oil from Nepeta ucrainica L. spp. kopetdaghensis. Ind. Crops Prod. 2014, 58, 315–321.Mencherini, T.; Picerno, P.; Scesa, C.; Aquino, R. Triterpene, antioxidant, and antimicrobial compounds from Melissa officinalis. J. Nat. Prod. 2007, 70, 1889–1894.González, R.E.; Restrepo, S.; Anaya, Y.; Zapateiro, L. Efecto de los recubrimientos binarios conteniendo extracto acuoso de laurel sobre la calidad pos cosecha de la fresa (Fragaria × ananassa). Inf. Tecnológica 2022, 33, 213–222Cazón, P.; Velázquez, G.; Ramírez, J.A.; Vázquez, M. Polysaccharide-based films and coatings for food packaging: A review. Food Hydrocoll. 2017, 68, 136–148.Michelin, M.; Marqués, M.; Pastrana, L.M.; Teixeira, J.A.; Cerqueira, M.A. Carboxymethyl cellulose-based films: Effect of organosolv lignin incorporation on physicochemical and antioxidant properties. J. Food Eng. 2020, 285, 110107.Singh, A.P.; Biswas, A.; Shukla, A.; Maiti, P. Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles. Signal Transduct. Target. Ther. 2019, 4, 33.Eduardo, P.; Villanueva, F. Encapsulation of food active ingredients in liposomes. J. Nutr. Health Food Eng. 2018, 8, 238–239Liu, X.; Bourvellec, C.L.; Renard, C.M. Interactions between cell wall polysaccharides and polyphenols: Effect of molecular internal structure. Compr. Rev. Food Sci. Food Saf. 2020, 19, 3574–3617.Guo, D.; Liu, J.; Fan, Y.; Cheng, J.; Shi, Y.; Zou, J.; Zhang, X. Optimization, characterization and evaluation of liposomes from Malus hupehensis (Pamp.) Rehd. Extracts. J. Liposome Res. 2020, 30, 366–376Shakir, M.; Ejaz, S.; Hussain, S.; Ali, S.; Sardar, H.; Azam, M.; Ullah, S.; Khaliq, G.; Saleem, M.; Nawaz, A.; et al. Synergistic effect of gum Arabic and carboxymethyl cellulose as biocomposite coating delays senescence in stored tomatoes by regulating antioxidants and cell wall degradation. Int. J. Biol. Macromol. 2022, 201, 641–652Li, P.; Yin, F.; Song, L.; Zheng, X. Alleviation of chilling injury in tomato fruit by exogenous application of oxalic acid. Food Chem. 2016, 202, 125–132.Velikova, V.; Loreto, F. On the relationship between isoprene emission and thermotolerance in Phragmites australis leaves exposed to high temperatures and during the recovery from a heat stress. Plant Cell Environ. 2005, 28, 318–327Karina, R.L.; Olga, M.B.; Robert, S.F. Effect of pulsed light treatments on quality and antioxidant properties of fresh-cut strawberries. Food Chem. 2018, 264, 393–400Brand-Williams, W.; Cuvelier, M.E.; Berset, C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci. Technol. 1995, 28, 25–30.Iñiguez-Moreno, M.C.; Ragazzo-Sánchez, J.A.; Barros-Castillo, J.C.; Sandoval-Contreras, T.; Calderón-Santoyo, M. Sodium alginate coatings added with Meyerozyma caribbica: Postharvest biocontrol of Colletotrichum gloeosporioides in avocado (Persea americana Mill. cv. Hass). Postharvest Biol. Technol. 2020, 163, 111123Gurjar, P.S.; Killadi, B.; Lenka, J.; Shukla, D.K. Effect of gum arabic coatings on physico-chemical and sensory qualities of guava (Psidium guajava L) cv. Shweta. Int. J. Curr. Microbiol. Appl. Sci. 2018, 7, 3769–3775.Ali, A.; Maqbool, M.; Ramachandran, S.; Alderson, P. Gum arabic as a novel edible coating for enhancing shelf-life and improving postharvest quality of tomato (Solanum lycopersicum L.) fruit. Postharvest Biol. Technol. 2010, 58, 42–47.Zhang, X.; Liu, X.; Du, M.; Tian, Y. Effect of polysaccharide derived from Osmunda japonica Thunb-incorporated carboxymethyl cellulose coatings on preservation of tomatoes. J. Food Process. Preserv. 2019, 43, 18.Ahmed, M.; Saini, P.; Iqbal, U. Bio cellulose-based edible composite coating for shelf-life extension of tomatoes. Food Humanit. 2023, 1, 973–984.Etemadipoor, R.; Dastjerdi, M.; Ramezanian, A.; Ehteshami, S. Ameliorative effect of gum arabic, oleic acid and/or cinnamon essential oil on chilling injury and quality loss of guava fruit. Sci. Hortic. 2020, 266, 109255Jhanani, G.K.; AlSalhi, M.; Naveena, T.; Shanmuganathan, R. As assessment of shelf life increasing competence of pectin (Zucchini) based edible coating on tomatoes. Environ. Res. 2024, 119368Anthon, G.E.; Lestrange, M.; Barrett, D.M. Changes in pH, acids, sugars and other quality parameters during extended vine holding of ripe processing tomatoes. J. Sci. Food Agric. 2011, 91, 1175–1181.Kumar, N.; Pratibha, N.; Neeraj, N.; Ojha, A.; Upadhyay, A.; Singh, R.; Kumar, S. Effect of active chitosan-pullulan composite edible coating enrich with pomegranate peel extract on the storage quality of green bell pepper. LWT Food Sci. Technol. 2021, 138, 110435Wardak, M.; Nkede, F.; Van, T.; Meng, F.; Tanaka, F.; Tanaka, F. Development of edible films and partial coating, a novel coating technique for tomato fruits, using citric acid-crosslinked starch and cellulose nanofiber. Prog. Org. Coat. 2024, 187, 108127Carrillo-Lomelí, D.A.; Cerqueira, M.A.; Moo-Huchin, V.; Bourbon, A.; Souza, V.; Lestido-Cardama, A.; Pastrana, L.; Ochoa-Fuentes, Y.; Hernández-Castillo, D.; Villarreal-Quintanilla, J.; et al. Influence of edible multilayer coatings with Opuntia stenopetala polysaccharides and Flourensia microphylla extract on the shelf-life of cherry tomato (Solanum lycopersicum L.). Sci. Hortic. 2024, 332, 113224Rives-Castillo, S.; Ventura-Aguilar, R.; Hernández-López, M.; Bautista-Baños, S. Evaluación de recubrimientos biodegradables para la conservación en fresco de jitomate Kenton. Acta Agrícola Pecu. 2018, 4, 80–91.da Silva, A.C.; Rodrigues Barbosa, J.; da Silva Araújo, C.; Sousa Batista, J.; Xavier Neves, E.; Pereira Cardoso, D.; Peixoto Joele, M.; Henriques Lourenço, L. A new edible coating of fish gelatin incorporated into açaí oil to increase the post-harvest shelf life of tomatoes. Food Chem. 2024, 438, 138047Pasquariello, M.; Di Patre, D.; Mastrobuoni, F.; Zampella, L.; Scortichini, M.; Petriccione, M. Influence of postharvest chitosan treatment on enzymatic browning and antioxidant enzyme activity in sweet cherry fruit. Postharvest Biol. Technol. 2015, 109, 45–56Ali, S.; Anjum, M.A.; Nawaz, A.; Naz, S.; Ejaz, S.; Sardar, H.; Saddiq, B. Tragacanth gum coating modulates oxidative stress and maintains quality of harvested apricot fruits. Int. J. Biol. Macromol. 2020, 163, 2439–2447.Chaple, S.; Vishwasrao, C.; Ananthanarayan, L. Edible composite coating of methyl cellulose for postharvest extension of shelf-life of finger hot indian pepper (Pusa jwala). J. Food Process. Preserv. 2017, 41, e12807.Ali, S.; Anjum, M.A.; Ejaz, S.; Hussain, S.; Ercisli, S.; Saleem, M.; Sardar, H. Carboxymethyl cellulose coating delays chilling injury development and maintains eating quality of ‘Kinnow’ mandarin fruits during low temperature storage. Int. J. Biol. Macromol. 2021, 168, 77–85.Khaliq, G.; Mohamed, M.T.; Ghazali, H.M.; Ding, P.; Ali, A. Influence of gum arabic coating enriched with calcium chloride on physiological, biochemical and quality responses of mango (Mangifera indica L.) fruit stored under low temperature stress. Postharvest Biol. Technol. 2016, 111, 362–369Tahir, H.E.; Zhihua, L.; Mahunu, G.; Xiaobo, Z.; Arslan, M.; Xiaowei, H.; Yang, Z.; Mariod, A. Effect of gum arabic edible coating incorporated with African baobab pulp extract on postharvest quality of cold stored blueberries. Food Sci. 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