Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia
Soybean crops grown in the plains of eastern Colombia have been affected by the incidence of rust. This disease, still officially regarded as American soybean rust (caused by Phakopsora meibomiae) by the Colombian Department of Agriculture (ICA), causes serious damage to soybean growing areas. Sympt...
- Autores:
-
Tapiero-Ortiz, Anibal Leonidas
López-Cardona, Nathali
Guevara-Castro, Alejandra
Rodríguez-Triana, Sandra Milena
Chavarro-Mesa, Edisson
Ángel-Diaz, Jorge Evelio
- Tipo de recurso:
- Fecha de publicación:
- 2021
- Institución:
- Universidad Tecnológica de Bolívar
- Repositorio:
- Repositorio Institucional UTB
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.utb.edu.co:20.500.12585/10667
- Palabra clave:
- Glycine max
Phakopsora spp. phenotyping
Phylogenetic analyses
Maximum likelihood method
Nucleotide sequencing
ITS region
LEMB
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/4.0/
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Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia |
| title |
Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia |
| spellingShingle |
Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia Glycine max Phakopsora spp. phenotyping Phylogenetic analyses Maximum likelihood method Nucleotide sequencing ITS region LEMB |
| title_short |
Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia |
| title_full |
Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia |
| title_fullStr |
Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia |
| title_full_unstemmed |
Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia |
| title_sort |
Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia |
| dc.creator.fl_str_mv |
Tapiero-Ortiz, Anibal Leonidas López-Cardona, Nathali Guevara-Castro, Alejandra Rodríguez-Triana, Sandra Milena Chavarro-Mesa, Edisson Ángel-Diaz, Jorge Evelio |
| dc.contributor.author.none.fl_str_mv |
Tapiero-Ortiz, Anibal Leonidas López-Cardona, Nathali Guevara-Castro, Alejandra Rodríguez-Triana, Sandra Milena Chavarro-Mesa, Edisson Ángel-Diaz, Jorge Evelio |
| dc.subject.keywords.es_CO.fl_str_mv |
Glycine max Phakopsora spp. phenotyping Phylogenetic analyses Maximum likelihood method Nucleotide sequencing ITS region |
| topic |
Glycine max Phakopsora spp. phenotyping Phylogenetic analyses Maximum likelihood method Nucleotide sequencing ITS region LEMB |
| dc.subject.armarc.none.fl_str_mv |
LEMB |
| description |
Soybean crops grown in the plains of eastern Colombia have been affected by the incidence of rust. This disease, still officially regarded as American soybean rust (caused by Phakopsora meibomiae) by the Colombian Department of Agriculture (ICA), causes serious damage to soybean growing areas. Symptoms of rust, such as reddish-brown lesions, have been observed since 2004 in the upper half of plants during vegetative stages. In 2005 and 2018, infected leaf tissues and uredinospores were collected from an experimental area and from commercial soybean fields. Once morphological identification and Koch’s postulates confirmed the presence of Phakopsora spp., molecular characterization was performed to identify the pathogen associated with the disease to species level. This was based on standard procedures using internal transcribed spacer (ITS) regions of rDNA and PCR amplification with specific primers for Phakopsora meibomiae and P. pachyrhizi. The obtained sequences were BLASTed against GenBank/ NCBI data bank. Results indicated that P. pachyrhizi Sydow is in fact the causative agent of soybean rust in Colombia, considering the samples collected in 2005 and 2018. The ITS-rDNA sequences of P. pachyrhizi were deposited at GenBank under the accession numbers MK933723 to MK933731. This finding was reported to ICA, so that they could officially update the phytosanitary status of this soybean pathogen in Colombia. |
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2021 |
| dc.date.issued.none.fl_str_mv |
2021-03-26 |
| dc.date.accessioned.none.fl_str_mv |
2022-04-08T21:49:46Z |
| dc.date.available.none.fl_str_mv |
2022-04-08T21:49:46Z |
| dc.date.submitted.none.fl_str_mv |
2022-04-07 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/restrictedAccess |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.identifier.citation.es_CO.fl_str_mv |
Tapiero, Anibal & López Cardona, Nathali & Guevara-Castro, Alejandra & Rodríguez-Triana, Sandra & Mesa, Edisson & Ángel-Diaz, Jorge. (2021). Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia. Summa Phytopathologica. 47. 149-156. 10.1590/0100-5405/245533. |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12585/10667 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1590/0100-5405/245533 |
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Universidad Tecnológica de Bolívar |
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Repositorio Universidad Tecnológica de Bolívar |
| identifier_str_mv |
Tapiero, Anibal & López Cardona, Nathali & Guevara-Castro, Alejandra & Rodríguez-Triana, Sandra & Mesa, Edisson & Ángel-Diaz, Jorge. (2021). Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia. Summa Phytopathologica. 47. 149-156. 10.1590/0100-5405/245533. Universidad Tecnológica de Bolívar Repositorio Universidad Tecnológica de Bolívar |
| url |
https://hdl.handle.net/20.500.12585/10667 https://doi.org/10.1590/0100-5405/245533 |
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eng |
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eng |
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http://purl.org/coar/access_right/c_abf2 |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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8 Páginas |
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application/pdf |
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Colombia |
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Cartagena de Indias |
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Summa Phytopathol., Botucatu, - vol. 47, n° 3 p. 149-156, 2021 |
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Universidad Tecnológica de Bolívar |
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Tapiero-Ortiz, Anibal Leonidase0a638c1-b004-4dd5-a625-a9a7df4fe6afLópez-Cardona, Nathali19e67238-7a3f-41c2-a96c-8f8c39ba13c9Guevara-Castro, Alejandrad07a1e7e-19c8-433d-8e8d-0dc4a75324d4Rodríguez-Triana, Sandra Milenaf97c2059-b27f-4722-83ad-a6e113a4fdd4Chavarro-Mesa, Edisson800ba77d-129a-48f9-bc50-2a8c25be1208Ángel-Diaz, Jorge Evelio079d25da-ca80-40d6-a694-1c0aa5f3214dColombia2022-04-08T21:49:46Z2022-04-08T21:49:46Z2021-03-262022-04-07Tapiero, Anibal & López Cardona, Nathali & Guevara-Castro, Alejandra & Rodríguez-Triana, Sandra & Mesa, Edisson & Ángel-Diaz, Jorge. (2021). Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombia. Summa Phytopathologica. 47. 149-156. 10.1590/0100-5405/245533.https://hdl.handle.net/20.500.12585/10667https://doi.org/10.1590/0100-5405/245533Universidad Tecnológica de BolívarRepositorio Universidad Tecnológica de BolívarSoybean crops grown in the plains of eastern Colombia have been affected by the incidence of rust. This disease, still officially regarded as American soybean rust (caused by Phakopsora meibomiae) by the Colombian Department of Agriculture (ICA), causes serious damage to soybean growing areas. Symptoms of rust, such as reddish-brown lesions, have been observed since 2004 in the upper half of plants during vegetative stages. In 2005 and 2018, infected leaf tissues and uredinospores were collected from an experimental area and from commercial soybean fields. Once morphological identification and Koch’s postulates confirmed the presence of Phakopsora spp., molecular characterization was performed to identify the pathogen associated with the disease to species level. This was based on standard procedures using internal transcribed spacer (ITS) regions of rDNA and PCR amplification with specific primers for Phakopsora meibomiae and P. pachyrhizi. The obtained sequences were BLASTed against GenBank/ NCBI data bank. Results indicated that P. pachyrhizi Sydow is in fact the causative agent of soybean rust in Colombia, considering the samples collected in 2005 and 2018. The ITS-rDNA sequences of P. pachyrhizi were deposited at GenBank under the accession numbers MK933723 to MK933731. This finding was reported to ICA, so that they could officially update the phytosanitary status of this soybean pathogen in Colombia.As culturas de soja produzidas nas planícies do leste da Colômbia vem sendo afetadas pela incidência de ferrugem. A doença, ainda considerada oficialmente, pelo Departamento de Agricultura da Colômbia (ICA) como ferrugem americana (ocasionada pelo fungo Phakopsora meibomiae), causa sérios danos à produção nas áreas de cultivo da soja. Os sintomas têm sido observados desde 2004 na metade superior das plantas durante os estágios vegetativos, como lesões marrom-avermelhadas. Em 2005 e 2018, tecidos foliares infectados e uredinosporos foram coletados em uma área experimental e cultivos comerciais dessa cultura. Uma vez que a identificação morfológica e os postulados de Koch confirmaram a presença de Phakopsora spp., foi realizada uma caracterização molecular para se identificar, a nível de espécie, o patógeno associado à ferrugem da soja. Estes foram baseados em procedimentos padrão com espaçadores de transcrição internos (ITS) do rDNA e amplificação por PCR com primers específicos para P. meibomiae e P. pachyrhizi. As sequências obtidas foram verificados por meio do Blast no site do GenBank/ NCBI. Os resultados indicaram que Phakopsora pachyrhizi Sydow é, de fato, o agente causal da ferrugem da soja na Colômbia, considerando-se as amostras coletadas em 2005 e 2018. As sequências do ITS-rDNA de P. pachyrhizi foram depositadas com os números de acesso do GenBank MK933723 até MK933731. A informação foi repassada ao ICA, para que atualize oficialmente a situação fitossanitária desse patógeno da soja na Colombia.8 Páginasapplication/pdfenghttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://purl.org/coar/access_right/c_abf2Summa Phytopathol., Botucatu, - vol. 47, n° 3 p. 149-156, 2021Phakopsora pachyrhizi is the major pathogen associated with soybean rust in Colombiainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/restrictedAccesshttp://purl.org/coar/resource_type/c_2df8fbb1Glycine maxPhakopsora spp. phenotypingPhylogenetic analysesMaximum likelihood methodNucleotide sequencingITS regionLEMBCartagena de IndiasBarnes, C. W., Szabo, L. J., Bowersox, V. C. Identifying and quantifying Phakopsora pachyrhizi spores in rain. Phytopathology. Minnesota, USA. v. 99, n. 4, p. 328–338. 2009.Buriticá, P., Pardo-Cardona, V. M. Flora uredineana colombiana. Revista de La Academia Colombiana de Ciencias Exactas, Físicas y Naturales. Bogotá, Colombia. v. 20, n. 77, p. 183–236. 1996CORPOICA. Soya [glycine Max (l.) Merril] Alternativa Para Los Sistemas de Produccion de la Orinoquia Colombiana. Bogotá, Colombia. p. 217. 2006.Ferreira Maciel, T. E., Menezes Freire, M. C., de Almeida, Á. M. R., Oliveira, L. O. de. Molecular characterization of beta-tubulin from Phakopsora pachyrhizi, the causal agent of Asian soybean rust. Genetics and Molecular Biology. Rio de Janeiro, Brazil. v. 33, n. 2, p. 354–358. 2010.Frederick, R. D., Snyder, C. L., Peterson, G. L., Bonde, M. R. Polymerase chain reaction assays for the detection and discrimination of the soybean rust pathogens Phakopsora pachyrhizi and P. meibomiae. Phytopathology. Minnesota, USA. v. 92, n. 2, p. 217–227. 2002Godoy, C. V., Seixas, C. D. S., Soares, R. M., Marcelino-Guimarães, F. C., Meyer, M. C., Costamilan, L. M. Asian soybean rust in Brazil: past, present, and future. Pesquisa Agropecuária Brasileira. Brasilia, Brazil. v. 51, p. 407–421. 2016Hepperly, P. R., Victoria, J. Soybean rust on soybean. FAO Plant Protection Bulletin. v. 28, n. 2, p. 77. 1980.Instituto Colombiano Agropecuario. Resolución número 00106 del 13 de septiembre de 2004. Diario Oficial n. 45.677. Santiago de Cali, Colombia. 2004.Kimura, M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution. New York, USA. v. 16, n. 2, p. 111–120. 1980Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Oxford, UK. Molecular Biology and Evolution. v. 35, n. 6, p. 1547. 2018Miles, M. R., Bonde, M. R., Nester, S. E., Berner, D. K., Frederick, R. D., Hartman, G. L. Characterizing resistance to Phakopsora pachyrhizi in soybean. Plant Disease. Idaho, USA. v. 95, n. 5, p. 577–581. 2011.Ministerio de Agricultura y Desarrollo Rural. Soya. Dirección de Cadenas Agrícolas y Forestales. Bogotá, Colombia. 2019. https://sioc.minagricultura.gov.co/AlimentosBalanceados/Documentos/2019-03-30 Cifras Sectoriales Soya.pdfMorel, P. W. Anais II Congresso Brasilero de Soja e Mercosoja. Foz de Iguazu, Brazil. 2002.Murillo-Williams, A., Esker, P., Allen, T., Stone, C., Frederick, R. First report of Phakopsora pachyrhizi on soybean in Costa Rica. Plant Disease. Idaho, USA. v. 99, n. 3, p. 418. 2015.Ono, Y., Buriticá, P., Hennen, J. F. Delimitation of Phakopsora, Physopella and Cerotelium and their species on Leguminosae. Mycological Research. UK. v. 96, n. 10, p. 825–850. 1992Pasiecznik, N. M., Smith, I. M., Watson, G. W., Brunt, A. A., Ritchie, B., Charles, L. M. F. CABI/EPPO distribution maps of plant pests and plant diseases and their important role in plant quarantine. Eppo Bulletin. UK. v. 35, n. 1, p. 1–7. 2005.Rossi, R. L. First report of Phakopsora pachyrhizi, the causal organism of soybean rust in the province of Misiones, Argentina. Plant Disease. Idaho, USA. v. 87, n. 1, p. 102. 2003. https://doi.org/10.1094/PDIS.2003.87.1.102ARupe, J., Sconyers, L. Soybean rust. The plant health instructor. American Phytopathology Society. Minnesota, USA. 2008. https://doi.org/10.1094/ PHI-I-2008-0401-01Sousa, P. F. C., Alves, E., Castro, H. A. de, Souza, P. E. de, Almeida, Á. M. R. de, Hochenbach, S. R. Distribuição e identificação de Phakopsora pachyrhizi, agente causal da ferrugem da soja no Estado de Minas Gerais. Ciência e Agrotecnologia. Londrina, Brazil. v. 31, p. 672–677. 2007. https://doi.org/10.1590/S1413-70542007000300011Udayanga, D., Castlebury, L. A., Rossman, A. Y., Chukeatirote, E., Hyde, K. D. The Diaporthe sojae species complex: Phylogenetic re-assessment of pathogens associated with soybean, cucurbits and other field crops. Fungal Biology. UK. v. 119, n. 5, p. 383–407. 2015.Villavicencio, A., Fanaro, G. B., Araújo, M. M., Aquino, S., Silva, P. V, Mancini-Filho, J. Detection of Phakopsora pachyrhizi by polymerase chain reaction (PCR) and use of germination test and DNA comet assay after e-beam processing in soybean. Radiation Physics and Chemistry. UK. v. 76, n. 11–12, p. 1878–1881. 2007.Yamanaka, N., Yamaoka, Y., Kato, M., Lemos, N. G., Passianotto, A. L. de L., dos Santos, J. V. M., Benitez, E. R., Abdelnoor, R. V, Soares, R. M., Suenaga, K. Development of classification criteria for resistance to soybean rust and differences in virulence among Japanese and Brazilian rust populations. Tropical Plant Pathology. Switzerland. v. 35, p. 153–162. 2010Zhang, X. C., Freire, M. C. M., Le, M. H., De Oliveira, L. O., Pitkin, J. W., Segers, G., Concibido, V. C., Baley, G. J., Hartman, G. L., Upchurch, G. Genetic diversity and origins of Phakopsora pachyrhizi isolates in the United States. Asian Journal of Plant Pathology. 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