Análisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximación
Las diferentes condiciones que experimenta Caretta caretta durante su ciclo de vida, dada su longevidad y capacidad de migrar grandes distancias, se refleja en cambios cualitativos y cuantitativos en la expresión de genes en las diferentes etapas de vida de la especie que pueden revelarse a través d...
- Autores:
- Tipo de recurso:
- Tesis
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Bogotá Jorge Tadeo Lozano
- Repositorio:
- Expeditio: repositorio UTadeo
- Idioma:
- spa
- OAI Identifier:
- oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/31047
- Acceso en línea:
- http://hdl.handle.net/20.500.12010/31047
http://expeditio.utadeo.edu.co
- Palabra clave:
- Tortugas marinas -- Sangre -- Análisis -- Santa Marta (Magdalena, Colombia)
Tortugas marinas -- Especies -- Santa Marta (Magdalena, Colombia)
Caretta caretta -- Análisis
Caretta caretta -- Tesis y disertaciones académicas
Tortugas marinas -- Especies -- Tesis y disertaciones académicas
Marine turtles -- Reproductive aspects
Marine turtles -- Research
- Rights
- License
- Acceso restringido
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| dc.title.spa.fl_str_mv |
Análisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximación |
| dc.title.alternative.none.fl_str_mv |
In silico analysis and identification of genes expressed differentially between juveniles and adults of the loggerhead turtle (caretta caretta) related to hypoxia and inmune system: first approach |
| title |
Análisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximación |
| spellingShingle |
Análisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximación Tortugas marinas -- Sangre -- Análisis -- Santa Marta (Magdalena, Colombia) Tortugas marinas -- Especies -- Santa Marta (Magdalena, Colombia) Caretta caretta -- Análisis Caretta caretta -- Tesis y disertaciones académicas Tortugas marinas -- Especies -- Tesis y disertaciones académicas Marine turtles -- Reproductive aspects Marine turtles -- Research |
| title_short |
Análisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximación |
| title_full |
Análisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximación |
| title_fullStr |
Análisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximación |
| title_full_unstemmed |
Análisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximación |
| title_sort |
Análisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximación |
| dc.contributor.advisor.none.fl_str_mv |
Hernández Fernández, Javier Adolfo |
| dc.subject.spa.fl_str_mv |
Tortugas marinas -- Sangre -- Análisis -- Santa Marta (Magdalena, Colombia) Tortugas marinas -- Especies -- Santa Marta (Magdalena, Colombia) Caretta caretta -- Análisis |
| topic |
Tortugas marinas -- Sangre -- Análisis -- Santa Marta (Magdalena, Colombia) Tortugas marinas -- Especies -- Santa Marta (Magdalena, Colombia) Caretta caretta -- Análisis Caretta caretta -- Tesis y disertaciones académicas Tortugas marinas -- Especies -- Tesis y disertaciones académicas Marine turtles -- Reproductive aspects Marine turtles -- Research |
| dc.subject.lemb.spa.fl_str_mv |
Caretta caretta -- Tesis y disertaciones académicas Tortugas marinas -- Especies -- Tesis y disertaciones académicas |
| dc.subject.keyword.spa.fl_str_mv |
Marine turtles -- Reproductive aspects Marine turtles -- Research |
| description |
Las diferentes condiciones que experimenta Caretta caretta durante su ciclo de vida, dada su longevidad y capacidad de migrar grandes distancias, se refleja en cambios cualitativos y cuantitativos en la expresión de genes en las diferentes etapas de vida de la especie que pueden revelarse a través del análisis del transcriptoma. En este estudio se realizó un análisis de expresión diferencial de genes, comparando transcriptomas de tortugas adultas (Adul) y tortugas juveniles (Juv), y se relacionaron con hipoxia y respuesta inmune. Las secuencias usadas corresponden a tortugas en cautiverio anidantes del Caribe colombiano, disponibles en GenBank, las cuales se filtraron (Trimmomatic), alinearon, mapearon (HISAT2) y ensamblaron (StringTie) contra un transcriptoma de referencia. El nivel de expresión de las lecturas de cada transcriptoma se cuantificó (featureCounts) para el análisis de expresión diferencial (DESeq2) y los genes se anotaron funcionalmente (Blast2GO-OmicsBox). Se mapearon correctamente el 84 % de las lecturas, y de la comparación Adul versus Juv, se identificaron 1401 genes expresados diferencialmente (DEG) (p-aj < 0,05), 507 regulados al alza y 894 a la baja (log₂ fold-change). Se logró anotar funcionalmente el 40 % de los DEG, identificando 8252 términos GO y 583 rutas de referencia de la ontología KEGG, en dónde sobresale la respuesta inmunológica, la respuesta al estrés oxidativo, y el metabolismo de carbohidratos. Se proponen posibles mecanismos y rutas metabólicas implicadas con la expresión de estos genes, según su función y el nivel de expresión en cada estadío. Lo planteado en las hipótesis sugeridas debe ser sometido a investigación desde enfoques más específicos que consideren la medición de variables no evaluadas en la presente investigación. |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-03-08T20:23:05Z |
| dc.date.available.none.fl_str_mv |
2023-03-08T20:23:05Z |
| dc.date.created.none.fl_str_mv |
2023 |
| dc.type.local.spa.fl_str_mv |
Trabajo de grado de pregrado |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_46ec |
| format |
http://purl.org/coar/resource_type/c_46ec |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12010/31047 |
| dc.identifier.repourl.spa.fl_str_mv |
http://expeditio.utadeo.edu.co |
| url |
http://hdl.handle.net/20.500.12010/31047 http://expeditio.utadeo.edu.co |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma. PARGRAFO: En caso de presentarse cualquier reclamación o acción por parte de un tercero en cuanto a los derechos de autor sobre la obra en cuestión, EL AUTOR, asumirá toda la responsabilidad, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos la universidad actúa como un tercero de buena fe. EL AUTOR, autoriza a LA UNIVERSIDAD DE BOGOTA JORGE TADEO LOZANO, para que en los términos establecidos en la Ley 23 de 1982, Ley 44 de 1993, Decisión andina 351 de 1993, Decreto 460 de 1995 y demás normas generales sobre la materia, utilice y use la obra objeto de la presente autorización. POLITICA DE TRATAMIENTO DE DATOS PERSONALES. Declaro que autorizo previa y de forma informada el tratamiento de mis datos personales por parte de LA UNIVERSIDAD DE BOGOTÁ JORGE TADEO LOZANO para fines académicos y en aplicación de convenios con terceros o servicios conexos con actividades propias de la academia, con estricto cumplimiento de los principios de ley. Para el correcto ejercicio de mi derecho de habeas data cuento con la cuenta de correo protecciondatos@utadeo.edu.co, donde previa identificación podré solicitar la consulta, corrección y supresión de mis datosAcceso restringidohttp://purl.org/coar/access_right/c_f1cfHernández Fernández, Javier AdolfoRivera Forero, CatalinaBiólogo(s) marinoColombia2023-03-08T20:23:05Z2023-03-08T20:23:05Z2023http://hdl.handle.net/20.500.12010/31047http://expeditio.utadeo.edu.coLas diferentes condiciones que experimenta Caretta caretta durante su ciclo de vida, dada su longevidad y capacidad de migrar grandes distancias, se refleja en cambios cualitativos y cuantitativos en la expresión de genes en las diferentes etapas de vida de la especie que pueden revelarse a través del análisis del transcriptoma. En este estudio se realizó un análisis de expresión diferencial de genes, comparando transcriptomas de tortugas adultas (Adul) y tortugas juveniles (Juv), y se relacionaron con hipoxia y respuesta inmune. Las secuencias usadas corresponden a tortugas en cautiverio anidantes del Caribe colombiano, disponibles en GenBank, las cuales se filtraron (Trimmomatic), alinearon, mapearon (HISAT2) y ensamblaron (StringTie) contra un transcriptoma de referencia. El nivel de expresión de las lecturas de cada transcriptoma se cuantificó (featureCounts) para el análisis de expresión diferencial (DESeq2) y los genes se anotaron funcionalmente (Blast2GO-OmicsBox). Se mapearon correctamente el 84 % de las lecturas, y de la comparación Adul versus Juv, se identificaron 1401 genes expresados diferencialmente (DEG) (p-aj < 0,05), 507 regulados al alza y 894 a la baja (log₂ fold-change). Se logró anotar funcionalmente el 40 % de los DEG, identificando 8252 términos GO y 583 rutas de referencia de la ontología KEGG, en dónde sobresale la respuesta inmunológica, la respuesta al estrés oxidativo, y el metabolismo de carbohidratos. Se proponen posibles mecanismos y rutas metabólicas implicadas con la expresión de estos genes, según su función y el nivel de expresión en cada estadío. Lo planteado en las hipótesis sugeridas debe ser sometido a investigación desde enfoques más específicos que consideren la medición de variables no evaluadas en la presente investigación.#BiologíaMarinaRequerimientos de sistema: Adobe Acrobat ReaderThe different conditions experienced by Caretta caretta during their life cycle, due to its longevity and the ability to migrate long distances, are reflected in qualitative and quantitative changes in gene expression at different life stages of the species that can be revealed through transcriptome analysis. In this study, a differential gene expression analysis was performed, comparing transcriptomes of adult turtles (Adul) and juvenile turtles (Juv), and related to hypoxia and immune response. The sequences used correspond to captive nesting turtles from the Colombian Caribbean, available in GenBank, these sequences were filtered (Trimmomatic), aligned, mapped (HISAT2) and assembled (StringTie) against a reference transcriptome. The expression level of reads from each transcriptome were quantified (featureCounts) for differential expression analysis (DESeq2) and genes were functionally annotated (Blast2GO-OmicsBox). 84% of reads were correctly mapped, and from the Adul versus Juv comparison, 1401 differentially expressed genes (DEGs) were identified (p-aj < 0.05), 507 up-regulated and 894 down-regulated (log₂ fold-change). 40 % of the DEGs were functionally annotated, identifying 8252 GO terms and 583 reference pathways of the KEGG ontology, where the immune response, oxidative stress response, and carbohydrate metabolism stand out. Possible mechanisms and metabolic pathways involved with the expression of these genes are proposed, according to their function and level of expression in each stage. The suggested hypotheses should be subjected to research from more specific approaches that consider the measurement of variables not evaluated in the present investigation.31 páginasapplication/pdf1 recurso en línea (archivo de texto)spaUniversidad de Bogotá Jorge Tadeo LozanoBiología marinaFacultad de Ciencias Naturales e IngenieríaUniversidad de Bogotá Jorge Tadeo LozanoExpeditio Repositorio Institucional UJTLTortugas marinas -- Sangre -- Análisis -- Santa Marta (Magdalena, Colombia)Tortugas marinas -- Especies -- Santa Marta (Magdalena, Colombia)Caretta caretta -- AnálisisCaretta caretta -- Tesis y disertaciones académicasTortugas marinas -- Especies -- Tesis y disertaciones académicasMarine turtles -- Reproductive aspectsMarine turtles -- ResearchAnálisis e identificación in silico de los genes expresados diferencialmente entre juveniles y adultos de la tortuga Caguama (Caretta caretta) relacionados con hipoxia y sistema inmune: primera aproximaciónIn silico analysis and identification of genes expressed differentially between juveniles and adults of the loggerhead turtle (caretta caretta) related to hypoxia and inmune system: first approachTrabajo de grado de pregradohttp://purl.org/coar/resource_type/c_46ecAfgan, E., D. 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