Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax

El gorgojo andino de la papa, Premnotrypes vorax, un insecto del orden Coleoptera, es una de las principales causas de daño a los cultivos de papa en las regiones andinas de América del Sur. Las proteínas Cry insecticidas de Bacillus thuringiensis son pesticidas biológicos útiles, y algunas son tóxi...

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Autores:
Tipo de recurso:
Article of journal
Fecha de publicación:
2000
Institución:
Universidad de Bogotá Jorge Tadeo Lozano
Repositorio:
Expeditio: repositorio UTadeo
Idioma:
spa
OAI Identifier:
oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/34173
Acceso en línea:
https://www.researchgate.net/publication/12210874_Recombinant_Cry3Aa_Has_Insecticidal_Activity_against_the_Andean_Potato_Weevil_Premnotrypes_vorax
http://hdl.handle.net/20.500.12010/34173
Palabra clave:
Cry3Aa
Cry3A
Premnotrypes vorax
Gorgojo andino de la papa
Bacillus thuringiensis
Gorgojo andino de la papa -- Premnotrypes vorax -- Control biológico
Proteínas Cry -- Pesticidas biológicos
Manejo integrado de plagas -- Métodos de contol
Cultivo de papa -- Biotecnología agrícola
Cry3Aa
Cry3A
Premnotrypes vorax
Andean potato weevil
Bacillus thuringiensis
Rights
License
Abierto (Texto Completo)
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network_name_str Expeditio: repositorio UTadeo
repository_id_str
dc.title.spa.fl_str_mv Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax
title Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax
spellingShingle Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax
Cry3Aa
Cry3A
Premnotrypes vorax
Gorgojo andino de la papa
Bacillus thuringiensis
Gorgojo andino de la papa -- Premnotrypes vorax -- Control biológico
Proteínas Cry -- Pesticidas biológicos
Manejo integrado de plagas -- Métodos de contol
Cultivo de papa -- Biotecnología agrícola
Cry3Aa
Cry3A
Premnotrypes vorax
Andean potato weevil
Bacillus thuringiensis
title_short Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax
title_full Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax
title_fullStr Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax
title_full_unstemmed Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax
title_sort Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax
dc.subject.spa.fl_str_mv Cry3Aa
Cry3A
Premnotrypes vorax
Gorgojo andino de la papa
Bacillus thuringiensis
topic Cry3Aa
Cry3A
Premnotrypes vorax
Gorgojo andino de la papa
Bacillus thuringiensis
Gorgojo andino de la papa -- Premnotrypes vorax -- Control biológico
Proteínas Cry -- Pesticidas biológicos
Manejo integrado de plagas -- Métodos de contol
Cultivo de papa -- Biotecnología agrícola
Cry3Aa
Cry3A
Premnotrypes vorax
Andean potato weevil
Bacillus thuringiensis
dc.subject.lemb.spa.fl_str_mv Gorgojo andino de la papa -- Premnotrypes vorax -- Control biológico
Proteínas Cry -- Pesticidas biológicos
Manejo integrado de plagas -- Métodos de contol
Cultivo de papa -- Biotecnología agrícola
dc.subject.keyword.spa.fl_str_mv Cry3Aa
Cry3A
Premnotrypes vorax
Andean potato weevil
Bacillus thuringiensis
description El gorgojo andino de la papa, Premnotrypes vorax, un insecto del orden Coleoptera, es una de las principales causas de daño a los cultivos de papa en las regiones andinas de América del Sur. Las proteínas Cry insecticidas de Bacillus thuringiensis son pesticidas biológicos útiles, y algunas son tóxicas para los insectos coleópteros. Se sobreexpresó la proteína Cry3Aa recombinante etiquetada con histidina en células hospedadoras de Escherichia coli. La proteína recombinante se solubilizó a un pH alto con urea, se purificó utilizando resina de afinidad de nitrilotriacetato de Ni^21^ y se dializó para bajar el pH y eliminar la urea. Se realizaron bioensayos con un medio para insectos cuya superficie estaba impregnada con 70 mg/mL de toxinas nativas o recombinantes purificadas. Las larvas de primer estadio expuestas al medio tratado con toxina durante 5 días exhibieron mortalidades del 57% (Cry3Aa nativa) al 52% (Cry3Aa recombinante). Las proteínas Cry3Aa nativas y recombinantes purificadas parecieron ser igualmente tóxicas para el gorgojo andino de la papa.
publishDate 2000
dc.date.created.none.fl_str_mv 2000-11
dc.date.accessioned.none.fl_str_mv 2024-04-03T10:02:20Z
dc.date.available.none.fl_str_mv 2024-04-03T10:02:20Z
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
format http://purl.org/coar/resource_type/c_6501
dc.identifier.other.spa.fl_str_mv https://www.researchgate.net/publication/12210874_Recombinant_Cry3Aa_Has_Insecticidal_Activity_against_the_Andean_Potato_Weevil_Premnotrypes_vorax
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12010/34173
url https://www.researchgate.net/publication/12210874_Recombinant_Cry3Aa_Has_Insecticidal_Activity_against_the_Andean_Potato_Weevil_Premnotrypes_vorax
http://hdl.handle.net/20.500.12010/34173
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.references.spa.fl_str_mv 1. Alcazar, J., and Cisneros, F. (posted 1996) Integrated Management for Andean potato weevils in pilot units. The International Potato Center (CIP). Available at http://www.cipotato.org/new/ WebProRep96/program4/prog43.htm.
2. Bravo, A. (1997) Phylogenetic relationships of Bacillus thuringiensis delta-endotoxin family proteins and their functional domains. J. Bacteriol. 179, 2793–2801
3. Hofte, H., and Whiteley, H. R. (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53, 242–255.
4. Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R., and Dean, D. H. (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62, 775–806.
5. Crickmore, N. 21 (posted July 1999) Bt Toxin Nomenclature. Available at http://www.biols.susx.ac.uk/Home/Neil_Crickmore/ Bt/index.hml.
7. Hofte, H., Seurinck, J., Van Houtven, A., and Vaeck, M. (1987) Nucleotide sequence of a gene encoding an insecticidal protein of Bacillus thuringiensis var. tenebrionis toxic against Coleoptera. Nucleic. Acids. Res. 15, 7183.
8. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
9. Koller, C. N., Bauer, L. S., and Hollingworth, R. M. (1992) Characterization of the pH-mediated solubility of Bacillus thuringiensis var. san diego native delta endotoxin crystal. Biochim. Biophys. Res. Comm. 184, 692–699.
10. Grayson, D. R., Lee, L., and Evans, D. R. (1985) Immunochemical analysis of the domain structure of CAD, the multifunctional protein that initiates pyrimidine biosynthesis in mammalian cells. J. Biol. Chem. 260, 15840–15849.
11. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.local.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 653–656 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Biochemical and Biophysical Research Communications Volumen 279
institution Universidad de Bogotá Jorge Tadeo Lozano
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https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/34173/2/license.txt
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spelling 2024-04-03T10:02:20Z2024-04-03T10:02:20Z2000-11https://www.researchgate.net/publication/12210874_Recombinant_Cry3Aa_Has_Insecticidal_Activity_against_the_Andean_Potato_Weevil_Premnotrypes_voraxhttp://hdl.handle.net/20.500.12010/34173El gorgojo andino de la papa, Premnotrypes vorax, un insecto del orden Coleoptera, es una de las principales causas de daño a los cultivos de papa en las regiones andinas de América del Sur. Las proteínas Cry insecticidas de Bacillus thuringiensis son pesticidas biológicos útiles, y algunas son tóxicas para los insectos coleópteros. Se sobreexpresó la proteína Cry3Aa recombinante etiquetada con histidina en células hospedadoras de Escherichia coli. La proteína recombinante se solubilizó a un pH alto con urea, se purificó utilizando resina de afinidad de nitrilotriacetato de Ni^21^ y se dializó para bajar el pH y eliminar la urea. Se realizaron bioensayos con un medio para insectos cuya superficie estaba impregnada con 70 mg/mL de toxinas nativas o recombinantes purificadas. Las larvas de primer estadio expuestas al medio tratado con toxina durante 5 días exhibieron mortalidades del 57% (Cry3Aa nativa) al 52% (Cry3Aa recombinante). Las proteínas Cry3Aa nativas y recombinantes purificadas parecieron ser igualmente tóxicas para el gorgojo andino de la papa.#Cry3Aa#Recombinante#Insecticida#Gorgojoandino#Papa#PremnotrypesvoraxThe Andean potato weevil, Premnotrypes vorax, an insect of the order Coleoptera, is a major cause of damage to potato crops in the Andean regions of South America. The insecticidal Cry proteins from Bacillus thuringiensis are useful biological pesticides, and some are toxic to Coleopteran insects. We overexpressed recombinant, histidine-tagged Cry3Aa protein in Escherichia coli host cells. The recombinant protein was solubilized at high pH with urea, purified using Ni21-nitrilo-triacetic acid affinity resin, and dialysed to lower pH and remove urea. Bioassays were performed with an insect media whose surface was spread with 70 mg/mL purified native or recombinant toxins. First instar larvae exposed to toxin treated media for 5 days exhibited mortalities from 57% (native Cry3Aa) to 52% (recombinant Cry3Aa). Purified native and recombinant Cry3Aa proteins appeared to be equally toxic to the Andean potato weevil.653–656 páginasapplication/pdfspaBiochemical and Biophysical Research Communications Volumen 279Cry3AaCry3APremnotrypes voraxGorgojo andino de la papaBacillus thuringiensisGorgojo andino de la papa -- Premnotrypes vorax -- Control biológicoProteínas Cry -- Pesticidas biológicosManejo integrado de plagas -- Métodos de contolCultivo de papa -- Biotecnología agrícolaCry3AaCry3APremnotrypes voraxAndean potato weevilBacillus thuringiensisRecombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes VoraxAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf21. Alcazar, J., and Cisneros, F. (posted 1996) Integrated Management for Andean potato weevils in pilot units. The International Potato Center (CIP). Available at http://www.cipotato.org/new/ WebProRep96/program4/prog43.htm.2. Bravo, A. (1997) Phylogenetic relationships of Bacillus thuringiensis delta-endotoxin family proteins and their functional domains. J. Bacteriol. 179, 2793–28013. Hofte, H., and Whiteley, H. R. (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53, 242–255.4. Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R., and Dean, D. H. (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62, 775–806.5. Crickmore, N. 21 (posted July 1999) Bt Toxin Nomenclature. Available at http://www.biols.susx.ac.uk/Home/Neil_Crickmore/ Bt/index.hml.7. Hofte, H., Seurinck, J., Van Houtven, A., and Vaeck, M. (1987) Nucleotide sequence of a gene encoding an insecticidal protein of Bacillus thuringiensis var. tenebrionis toxic against Coleoptera. Nucleic. Acids. Res. 15, 7183.8. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.9. Koller, C. N., Bauer, L. S., and Hollingworth, R. M. (1992) Characterization of the pH-mediated solubility of Bacillus thuringiensis var. san diego native delta endotoxin crystal. Biochim. Biophys. Res. Comm. 184, 692–699.10. Grayson, D. R., Lee, L., and Evans, D. R. (1985) Immunochemical analysis of the domain structure of CAD, the multifunctional protein that initiates pyrimidine biosynthesis in mammalian cells. J. Biol. Chem. 260, 15840–15849.11. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.http://purl.org/coar/resource_type/c_6501Gómez, SylviaMateus, Ana ConstanzaHernández-Fernández, Javier AdolfoZimmermann, Barbara H.ORIGINALRecombinant.pdfRecombinant.pdfArchivo abierto / Open archiveapplication/pdf126966https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/34173/1/Recombinant.pdfc68d5c7bef8aa9c02f1a8c6f2aa6cab7MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-82938https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/34173/2/license.txtbaba314677a6b940f072575a13bb6906MD52open accessTHUMBNAILRecombinant.pdf.jpgRecombinant.pdf.jpgIM Thumbnailimage/jpeg6500https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/34173/3/Recombinant.pdf.jpgede15824bece1cbff6d466fef268b8a5MD53open access20.500.12010/34173oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/341732024-04-04 03:01:56.621open accessRepositorio Institucional - Universidad Jorge Tadeo Lozanoexpeditiorepositorio@utadeo.edu.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