Sample pooling on the Cepheid Xpert R Xpress SARS-CoV-2 assay
The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples...
- Autores:
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2020
- Institución:
- Universidad de Bogotá Jorge Tadeo Lozano
- Repositorio:
- Expeditio: repositorio UTadeo
- Idioma:
- eng
- OAI Identifier:
- oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/14514
- Acceso en línea:
- https://doi.org/10.1016/j.diagmicrobio.2020.115238
http://hdl.handle.net/20.500.12010/14514
- Palabra clave:
- Pooling
GeneXpert
Cepheid
SARS-CoV-2
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
- Rights
- License
- Abierto (Texto Completo)
| Summary: | The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of four and six. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had ‘neat’ Xpert® E gene cycle threshold (Ct) values ranging between 20 and 28 and all were detected qualitatively when contained in pools of four or six samples. For these samples, pooling had a median change in Ct value of 2.0 in pools of four, and of 2.9 in pools of six. With the use of Cepheid Xpert® Xpress SARSCoV-2 RT-PCR assay, pooling of four or six samples may be an effective strategy to increase testing capacity. |
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