Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle
Para comprender los cambios en la actividad enzimática y la expresión génica como biomarcadores de la exposición al metilmercurio, expusimos eritrocitos (GR) de tortuga boba a concentraciones de 0, 1 y 5 mg de MeHg. 5 mg L1 de MeHg y se ensamblaron transcriptomas de novo mediante RNA-seq. El análisi...
- Autores:
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2021
- Institución:
- Universidad de Bogotá Jorge Tadeo Lozano
- Repositorio:
- Expeditio: repositorio UTadeo
- Idioma:
- spa
- OAI Identifier:
- oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/32275
- Palabra clave:
- Tortugas marinas
Biología marina
Metilmercurio
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Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle |
| title |
Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle |
| spellingShingle |
Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle Tortugas marinas Biología marina Metilmercurio |
| title_short |
Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle |
| title_full |
Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle |
| title_fullStr |
Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle |
| title_full_unstemmed |
Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle |
| title_sort |
Transcriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea Turtle |
| dc.subject.spa.fl_str_mv |
Tortugas marinas Biología marina Metilmercurio |
| topic |
Tortugas marinas Biología marina Metilmercurio |
| description |
Para comprender los cambios en la actividad enzimática y la expresión génica como biomarcadores de la exposición al metilmercurio, expusimos eritrocitos (GR) de tortuga boba a concentraciones de 0, 1 y 5 mg de MeHg. 5 mg L1 de MeHg y se ensamblaron transcriptomas de novo mediante RNA-seq. El análisis de genes expresados diferencialmente (DEG) indicó que 79 genes únicos estaban desregulados (39 regulados al alza y 44 regulados a la baja). (39 al alza y 44 a la baja). Los resultados mostraron que el MeHg alteró los patrones de expresión génica como respuesta al estrés celular producido, reflejado en la regulación del ciclo celular, la actividad lisosomal autofagia, regulación del calcio, regulación mitocondrial, apoptosis y regulación de la transcripción y la traducción. y la traducción. El análisis de DEGs mostró una baja respuesta de la maquinaria antioxidante al MeHg, evidenciado por el hecho de que los genes de respuesta temprana al estrés oxidativo no estaban desregulados. Los RBCs mantuvieron una expresión constitutiva de proteínas que representaban una buena parte de la defensa contra las especies reactivas del oxígeno (ROS) inducidas por el MeHg. |
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2021 |
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2021 |
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2023-10-31T05:42:34Z |
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2023-10-31T05:42:34Z |
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https://www.mdpi.com/2305-6304/9/4/70 |
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http://hdl.handle.net/20.500.12010/32275 |
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https://www.mdpi.com/2305-6304/9/4/70 http://hdl.handle.net/20.500.12010/32275 |
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spa |
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spa |
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Facemire, C.; Augspurger, T.; Bateman, D.; Brim, M.; Conzelmann, P.; Delchamps, S.; Masson, G. Impacts of mercury contamination in the southeastern United States. In Mercury as a Global Pollutant; Springer: Dordrech, The Netherlands, 1995; pp. 923–926. Ajsuvakova, O.P.; Tinkov, A.A.; Aschner, M.; Rocha, J.B.; Michalke, B.; Skalnaya, M.G.; Aaseth, J. Sulfhydryl groups as targets of mercury toxicity. Coord. Chem. Rev. 2020, 417, 213–343. [CrossRef] [PubMed Fernández, A.; Singh, A.; Jaffé, R. A literature review on trace metals and organic compounds of anthropogenic origin in the Wider Caribbean Region. Mar. Pollute. Bull. 2007, 54, 1681–1691. [CrossRef] dos Santos, A.; Ferrer, B.; Marques Gonçalves, F.; Tsatsakis, A.M.; Renieri, E.; Skalny, A.; Aschner, M.A. Oxidative stress in methylmercury-induced cell toxicity. Toxics 2018, 6, 47. [CrossRef] [PubMed] Burger, J.; Gochfeld, M. Mercury in canned tuna: White versus light and temporal variation. Environ. Res. 2004, 96, 239–249. [CrossRef] Henny, C.J.; Hill, E.F.; Hoffman, D.J.; Spalding, M.G.; Grove, R.A. Nineteenth century mercury: Hazard to wading birds and cormorants of the Carson River, Nevada. Ecotoxicology 2002, 11, 213–231. [CrossRef] [PubMed] Bridges, C.C.; Zalups, R.K. Mechanisms involved in the transport of mercuric ions in target tissues. Arch. Toxicol. 2017, 91, 63–81. [CrossRef] Oliveira, C.S.; Piccoli, B.C.; Aschner, M.; Rocha, J.B.T. Chemical speciation of selenium and mercury as determinant of their neurotoxicity in Neurotoxicity of Metals. In Neurotoxicity of Metals. Advances in Neurobiology, 1st ed.; Springer: Cham, Switzerland, 2017; pp. 53–83. Wiener, J.G.; Krabbenhoft, D.P.; Heinz, G.H.; Scheuhammer, A.M. Ecotoxicology of Mercury. In Handbook of Ecotoxicology, 2nd ed.; Hoffman, D.J., Rattner, B.A., Burton, G.A., Cairns, J., Jr., Eds.; CRC Press: Boca Raton, FL, USA, 2003; pp. 409–463. Storelli, M.M.; Storelli, A.; D’Addabbo, R.; Marano, C.; Bruno, R.; Marcotrigiano, G.O. Trace elements in loggerhead turtles (Caretta caretta) from the eastern Mediterranean Sea: Overview and evaluation. Environ. Pollut. 2005, 135, 163–170. [CrossRef] |
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29 páginas |
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Toxics |
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2023-10-31T05:42:34Z2023-10-31T05:42:34Z2021https://www.mdpi.com/2305-6304/9/4/70http://hdl.handle.net/20.500.12010/32275Para comprender los cambios en la actividad enzimática y la expresión génica como biomarcadores de la exposición al metilmercurio, expusimos eritrocitos (GR) de tortuga boba a concentraciones de 0, 1 y 5 mg de MeHg. 5 mg L1 de MeHg y se ensamblaron transcriptomas de novo mediante RNA-seq. El análisis de genes expresados diferencialmente (DEG) indicó que 79 genes únicos estaban desregulados (39 regulados al alza y 44 regulados a la baja). (39 al alza y 44 a la baja). Los resultados mostraron que el MeHg alteró los patrones de expresión génica como respuesta al estrés celular producido, reflejado en la regulación del ciclo celular, la actividad lisosomal autofagia, regulación del calcio, regulación mitocondrial, apoptosis y regulación de la transcripción y la traducción. y la traducción. El análisis de DEGs mostró una baja respuesta de la maquinaria antioxidante al MeHg, evidenciado por el hecho de que los genes de respuesta temprana al estrés oxidativo no estaban desregulados. Los RBCs mantuvieron una expresión constitutiva de proteínas que representaban una buena parte de la defensa contra las especies reactivas del oxígeno (ROS) inducidas por el MeHg.#CarettaCarettaTo understand changes in enzyme activity and gene expression as biomarkers of exposure to methylmercury, we exposed loggerhead turtle erythrocytes (RBCs) to concentrations of 0, 1, and 5 mg L1 of MeHg and de novo transcriptome were assembled using RNA-seq. The analysis of differentially expressed genes (DEGs) indicated that 79 unique genes were dysregulated (39 upregulated and 44 downregulated genes). The results showed that MeHg altered gene expression patterns as a response to the cellular stress produced, reflected in cell cycle regulation, lysosomal activity, autophagy, calcium regulation, mitochondrial regulation, apoptosis, and regulation of transcription and translation. The analysis of DEGs showed a low response of the antioxidant machinery to MeHg, evidenced by the fact that genes of early response to oxidative stress were not dysregulated. The RBCs maintained a constitutive expression of proteins that represented a good part of the defense against reactive oxygen species (ROS) induced by MeHg.29 páginasapplication/pdfspaToxicsTortugas marinasBiología marinaMetilmercurioTranscriptional Analyses of Acute Exposure to Methylmercury on Erythrocytes of Loggerhead Sea TurtleAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Facemire, C.; Augspurger, T.; Bateman, D.; Brim, M.; Conzelmann, P.; Delchamps, S.; Masson, G. Impacts of mercury contamination in the southeastern United States. In Mercury as a Global Pollutant; Springer: Dordrech, The Netherlands, 1995; pp. 923–926.Ajsuvakova, O.P.; Tinkov, A.A.; Aschner, M.; Rocha, J.B.; Michalke, B.; Skalnaya, M.G.; Aaseth, J. Sulfhydryl groups as targets of mercury toxicity. Coord. Chem. Rev. 2020, 417, 213–343. [CrossRef] [PubMedFernández, A.; Singh, A.; Jaffé, R. A literature review on trace metals and organic compounds of anthropogenic origin in the Wider Caribbean Region. Mar. Pollute. Bull. 2007, 54, 1681–1691. [CrossRef]dos Santos, A.; Ferrer, B.; Marques Gonçalves, F.; Tsatsakis, A.M.; Renieri, E.; Skalny, A.; Aschner, M.A. Oxidative stress in methylmercury-induced cell toxicity. Toxics 2018, 6, 47. [CrossRef] [PubMed]Burger, J.; Gochfeld, M. Mercury in canned tuna: White versus light and temporal variation. Environ. Res. 2004, 96, 239–249. [CrossRef]Henny, C.J.; Hill, E.F.; Hoffman, D.J.; Spalding, M.G.; Grove, R.A. Nineteenth century mercury: Hazard to wading birds and cormorants of the Carson River, Nevada. Ecotoxicology 2002, 11, 213–231. [CrossRef] [PubMed]Bridges, C.C.; Zalups, R.K. Mechanisms involved in the transport of mercuric ions in target tissues. Arch. Toxicol. 2017, 91, 63–81. [CrossRef]Oliveira, C.S.; Piccoli, B.C.; Aschner, M.; Rocha, J.B.T. Chemical speciation of selenium and mercury as determinant of their neurotoxicity in Neurotoxicity of Metals. In Neurotoxicity of Metals. Advances in Neurobiology, 1st ed.; Springer: Cham, Switzerland, 2017; pp. 53–83.Wiener, J.G.; Krabbenhoft, D.P.; Heinz, G.H.; Scheuhammer, A.M. Ecotoxicology of Mercury. In Handbook of Ecotoxicology, 2nd ed.; Hoffman, D.J., Rattner, B.A., Burton, G.A., Cairns, J., Jr., Eds.; CRC Press: Boca Raton, FL, USA, 2003; pp. 409–463.Storelli, M.M.; Storelli, A.; D’Addabbo, R.; Marano, C.; Bruno, R.; Marcotrigiano, G.O. Trace elements in loggerhead turtles (Caretta caretta) from the eastern Mediterranean Sea: Overview and evaluation. Environ. Pollut. 2005, 135, 163–170. [CrossRef]http://purl.org/coar/resource_type/c_6501Hernández-Fernández, JavierPinzón-Velasco, AndrésAnne López, EllieRodríguez-Becerra, PilarMariño-Ramírez, LeonardoTHUMBNAILtoxics-09-00070-v5.pdf.jpgtoxics-09-00070-v5.pdf.jpgIM Thumbnailimage/jpeg21400https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/32275/3/toxics-09-00070-v5.pdf.jpg00b435857489c02785447380184bd4d0MD53open accessORIGINALtoxics-09-00070-v5.pdftoxics-09-00070-v5.pdfapplication/pdf8507267https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/32275/1/toxics-09-00070-v5.pdff8a369bf933ef8f3095bafa9ea613e04MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-82938https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/32275/2/license.txtbaba314677a6b940f072575a13bb6906MD52open access20.500.12010/32275oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/322752023-11-10 10:08:21.153open accessRepositorio Institucional - Universidad Jorge Tadeo Lozanoexpeditiorepositorio@utadeo.edu.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 |