Oxidative Stress Biomarkers in Erythrocytes of Captive Pre-Juvenile Loggerhead Turtles Following Acute Exposure to Methylmercury

This study describes the use of erythrocytes (RBCs) of loggerhead turtles as in vitro models for evaluating their toxicity to methylmercury. Blood samples of loggerhead turtles that were born in the Colombian Caribbean were used. The LC50 of RBCs to methylmercury was determined at 96 h using methylm...

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Autores:
Tipo de recurso:
Article of journal
Fecha de publicación:
2020
Institución:
Universidad de Bogotá Jorge Tadeo Lozano
Repositorio:
Expeditio: repositorio UTadeo
Idioma:
spa
OAI Identifier:
oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/27502
Acceso en línea:
https://www.researchgate.net/publication/341615546_Oxidative_Stress_Biomarkers_in_Erythrocytes_of_Captive_Pre-Juvenile_Loggerhead_Turtles_Following_Acute_Exposure_to_Methylmercury
http://hdl.handle.net/20.500.12010/27502
http://expeditiorepositorio.utadeo.edu.co
Palabra clave:
Cytotoxicity
Erythrocytes
Methylmercury
Genética animal
Tortugas
Marcadores bioquímicos
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Summary:This study describes the use of erythrocytes (RBCs) of loggerhead turtles as in vitro models for evaluating their toxicity to methylmercury. Blood samples of loggerhead turtles that were born in the Colombian Caribbean were used. The LC50 of RBCs to methylmercury was determined at 96 h using methylmercury concentrations of 0.5-100 mg L −1. Next, the viability of the RBCs and the activity of the enzymes superoxide dismutase (SOD), glutathione S-transferase (GST), and lipid peroxidation by malondialdehyde (MDA) at 6 and 12 h of exposure to acute concentrations of 0, 1, and 5 mg L −1 were evaluated. The LC50 for loggerhead turtle RBCs was 8.32 mg L −1. The cell viability bioassay of RBCs exposed for 12 h only showed 100% cell viability. Increasing in vitro MeHg concentrations caused a corresponding increase in MDA concentration as well as decreases in the activities of SOD and GST. The RBCs represent an excellent model for ecotoxicological studies and SOD, GST, and MDA are biomarkers of environmental pollution and oxidative stress in loggerhead turtles. This was the first study conducted on loggerhead turtle where the response of RBCs to MeHg-induced oxidative stress is evaluated.