Optogenetic Tools in the Molecular Spotlight
The rise of optogenetics as a standard technique to non-invasively probe and monitor biological function created an immense interest in the molecular function of photosensory proteins. These photoreceptors are usually protein/pigment complexes that translate light into biological information and hav...
- Autores:
- Tipo de recurso:
- Book
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Bogotá Jorge Tadeo Lozano
- Repositorio:
- Expeditio: repositorio UTadeo
- Idioma:
- eng
- OAI Identifier:
- oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/14337
- Acceso en línea:
- https://www.frontiersin.org/research-topics/3172/optogenetic-tools-in-the-molecular-spotlight
http://hdl.handle.net/20.500.12010/14337
- Palabra clave:
- Science (General)
Photoreceptors
Flavins
Phytochrome
Photochemistry and photophysics
Protein engineering
Computational modeling
Optogenetics
Opsins
Spectroscopy
- Rights
- License
- Abierto (Texto Completo)
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dc.title.spa.fl_str_mv |
Optogenetic Tools in the Molecular Spotlight |
title |
Optogenetic Tools in the Molecular Spotlight |
spellingShingle |
Optogenetic Tools in the Molecular Spotlight Science (General) Photoreceptors Flavins Phytochrome Photochemistry and photophysics Protein engineering Computational modeling Optogenetics Opsins Spectroscopy |
title_short |
Optogenetic Tools in the Molecular Spotlight |
title_full |
Optogenetic Tools in the Molecular Spotlight |
title_fullStr |
Optogenetic Tools in the Molecular Spotlight |
title_full_unstemmed |
Optogenetic Tools in the Molecular Spotlight |
title_sort |
Optogenetic Tools in the Molecular Spotlight |
dc.subject.spa.fl_str_mv |
Science (General) Photoreceptors Flavins Phytochrome |
topic |
Science (General) Photoreceptors Flavins Phytochrome Photochemistry and photophysics Protein engineering Computational modeling Optogenetics Opsins Spectroscopy |
dc.subject.lemb.spa.fl_str_mv |
Photochemistry and photophysics Protein engineering Computational modeling |
dc.subject.keyword.spa.fl_str_mv |
Optogenetics Opsins Spectroscopy |
description |
The rise of optogenetics as a standard technique to non-invasively probe and monitor biological function created an immense interest in the molecular function of photosensory proteins. These photoreceptors are usually protein/pigment complexes that translate light into biological information and have become essential tools in cell biology and neurobiology as their function is genetically encoded and can be conveniently delivered into a given cell. Like for fluorescent proteins that quickly became invaluable as genetically encodable reporters in microscopy and imaging, variants of photosensory proteins with customized sensitivity and functionality are nowadays in high demand. In this ebook we feature reviews and original research on molecular approaches from synthetic biology and molecular spectroscopy to computational molecular modelling that all aspire to elucidate the molecular prerequisites for the photosensory function of the given proteins. The principle property of changing activity of biological function simply by application of light is not only very attractive for cell biology, it also offers unique opportunities for molecular studies as excitation can be controlled with high time precision. Especially in spectroscopy the usually fully reversible photoactivation of photosensory proteins allows researchers to to perform time resolved studies with up to femtosecond resolution. In addition, functional variants can be investigated and quickly screened in common biochemical experiments. The insights that are obtained by the here presented various yet complementary methods will ultimately allow us write the script for a molecular movie from excitation of the protein by a photon to activation of its biological function. Such deep understanding does not only provide unique insights into the dynamics of protein function, it will also ultimately enable us to rationally design novel optogenetic tools to be used in cell biology and therapy. |
publishDate |
2016 |
dc.date.created.none.fl_str_mv |
2016-01-19 |
dc.date.accessioned.none.fl_str_mv |
2020-10-09T22:55:13Z |
dc.date.available.none.fl_str_mv |
2020-10-09T22:55:13Z |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2f33 |
format |
http://purl.org/coar/resource_type/c_2f33 |
dc.identifier.isbn.none.fl_str_mv |
978-2-889-19899-3 |
dc.identifier.issn.none.fl_str_mv |
1664-8714 |
dc.identifier.other.none.fl_str_mv |
https://www.frontiersin.org/research-topics/3172/optogenetic-tools-in-the-molecular-spotlight |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12010/14337 |
dc.identifier.doi.none.fl_str_mv |
10.3389/978-2-88919-899-3 |
identifier_str_mv |
978-2-889-19899-3 1664-8714 10.3389/978-2-88919-899-3 |
url |
https://www.frontiersin.org/research-topics/3172/optogenetic-tools-in-the-molecular-spotlight http://hdl.handle.net/20.500.12010/14337 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.references.none.fl_str_mv |
Mathes, T., Kennis, J. T. M., eds. (2016). Optogenetic Tools in the Molecular Spotlight. Lausanne: Frontiers Media. doi: 10.3389/978-2-88919-899-3 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.local.spa.fl_str_mv |
Abierto (Texto Completo) |
dc.rights.creativecommons.none.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
rights_invalid_str_mv |
Abierto (Texto Completo) https://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2 |
dc.format.extent.spa.fl_str_mv |
181 páginas |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Frontiers Media SA |
institution |
Universidad de Bogotá Jorge Tadeo Lozano |
bitstream.url.fl_str_mv |
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2020-10-09T22:55:13Z2020-10-09T22:55:13Z2016-01-19978-2-889-19899-31664-8714https://www.frontiersin.org/research-topics/3172/optogenetic-tools-in-the-molecular-spotlighthttp://hdl.handle.net/20.500.12010/1433710.3389/978-2-88919-899-3181 páginasapplication/pdfengFrontiers Media SAScience (General)PhotoreceptorsFlavinsPhytochromePhotochemistry and photophysicsProtein engineeringComputational modelingOptogeneticsOpsinsSpectroscopyOptogenetic Tools in the Molecular SpotlightAbierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2Mathes, T., Kennis, J. T. M., eds. (2016). Optogenetic Tools in the Molecular Spotlight. Lausanne: Frontiers Media. doi: 10.3389/978-2-88919-899-3The rise of optogenetics as a standard technique to non-invasively probe and monitor biological function created an immense interest in the molecular function of photosensory proteins. These photoreceptors are usually protein/pigment complexes that translate light into biological information and have become essential tools in cell biology and neurobiology as their function is genetically encoded and can be conveniently delivered into a given cell. Like for fluorescent proteins that quickly became invaluable as genetically encodable reporters in microscopy and imaging, variants of photosensory proteins with customized sensitivity and functionality are nowadays in high demand. In this ebook we feature reviews and original research on molecular approaches from synthetic biology and molecular spectroscopy to computational molecular modelling that all aspire to elucidate the molecular prerequisites for the photosensory function of the given proteins. The principle property of changing activity of biological function simply by application of light is not only very attractive for cell biology, it also offers unique opportunities for molecular studies as excitation can be controlled with high time precision. Especially in spectroscopy the usually fully reversible photoactivation of photosensory proteins allows researchers to to perform time resolved studies with up to femtosecond resolution. In addition, functional variants can be investigated and quickly screened in common biochemical experiments. The insights that are obtained by the here presented various yet complementary methods will ultimately allow us write the script for a molecular movie from excitation of the protein by a photon to activation of its biological function. Such deep understanding does not only provide unique insights into the dynamics of protein function, it will also ultimately enable us to rationally design novel optogenetic tools to be used in cell biology and therapy.http://purl.org/coar/resource_type/c_2f33Mathes, TiloKennis, John T. 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