Optogenetic Tools in the Molecular Spotlight

The rise of optogenetics as a standard technique to non-invasively probe and monitor biological function created an immense interest in the molecular function of photosensory proteins. These photoreceptors are usually protein/pigment complexes that translate light into biological information and hav...

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Autores:
Tipo de recurso:
Book
Fecha de publicación:
2016
Institución:
Universidad de Bogotá Jorge Tadeo Lozano
Repositorio:
Expeditio: repositorio UTadeo
Idioma:
eng
OAI Identifier:
oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/14337
Acceso en línea:
https://www.frontiersin.org/research-topics/3172/optogenetic-tools-in-the-molecular-spotlight
http://hdl.handle.net/20.500.12010/14337
Palabra clave:
Science (General)
Photoreceptors
Flavins
Phytochrome
Photochemistry and photophysics
Protein engineering
Computational modeling
Optogenetics
Opsins
Spectroscopy
Rights
License
Abierto (Texto Completo)
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oai_identifier_str oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/14337
network_acronym_str UTADEO2
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repository_id_str
dc.title.spa.fl_str_mv Optogenetic Tools in the Molecular Spotlight
title Optogenetic Tools in the Molecular Spotlight
spellingShingle Optogenetic Tools in the Molecular Spotlight
Science (General)
Photoreceptors
Flavins
Phytochrome
Photochemistry and photophysics
Protein engineering
Computational modeling
Optogenetics
Opsins
Spectroscopy
title_short Optogenetic Tools in the Molecular Spotlight
title_full Optogenetic Tools in the Molecular Spotlight
title_fullStr Optogenetic Tools in the Molecular Spotlight
title_full_unstemmed Optogenetic Tools in the Molecular Spotlight
title_sort Optogenetic Tools in the Molecular Spotlight
dc.subject.spa.fl_str_mv Science (General)
Photoreceptors
Flavins
Phytochrome
topic Science (General)
Photoreceptors
Flavins
Phytochrome
Photochemistry and photophysics
Protein engineering
Computational modeling
Optogenetics
Opsins
Spectroscopy
dc.subject.lemb.spa.fl_str_mv Photochemistry and photophysics
Protein engineering
Computational modeling
dc.subject.keyword.spa.fl_str_mv Optogenetics
Opsins
Spectroscopy
description The rise of optogenetics as a standard technique to non-invasively probe and monitor biological function created an immense interest in the molecular function of photosensory proteins. These photoreceptors are usually protein/pigment complexes that translate light into biological information and have become essential tools in cell biology and neurobiology as their function is genetically encoded and can be conveniently delivered into a given cell. Like for fluorescent proteins that quickly became invaluable as genetically encodable reporters in microscopy and imaging, variants of photosensory proteins with customized sensitivity and functionality are nowadays in high demand. In this ebook we feature reviews and original research on molecular approaches from synthetic biology and molecular spectroscopy to computational molecular modelling that all aspire to elucidate the molecular prerequisites for the photosensory function of the given proteins. The principle property of changing activity of biological function simply by application of light is not only very attractive for cell biology, it also offers unique opportunities for molecular studies as excitation can be controlled with high time precision. Especially in spectroscopy the usually fully reversible photoactivation of photosensory proteins allows researchers to to perform time resolved studies with up to femtosecond resolution. In addition, functional variants can be investigated and quickly screened in common biochemical experiments. The insights that are obtained by the here presented various yet complementary methods will ultimately allow us write the script for a molecular movie from excitation of the protein by a photon to activation of its biological function. Such deep understanding does not only provide unique insights into the dynamics of protein function, it will also ultimately enable us to rationally design novel optogenetic tools to be used in cell biology and therapy.
publishDate 2016
dc.date.created.none.fl_str_mv 2016-01-19
dc.date.accessioned.none.fl_str_mv 2020-10-09T22:55:13Z
dc.date.available.none.fl_str_mv 2020-10-09T22:55:13Z
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2f33
format http://purl.org/coar/resource_type/c_2f33
dc.identifier.isbn.none.fl_str_mv 978-2-889-19899-3
dc.identifier.issn.none.fl_str_mv 1664-8714
dc.identifier.other.none.fl_str_mv https://www.frontiersin.org/research-topics/3172/optogenetic-tools-in-the-molecular-spotlight
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12010/14337
dc.identifier.doi.none.fl_str_mv 10.3389/978-2-88919-899-3
identifier_str_mv 978-2-889-19899-3
1664-8714
10.3389/978-2-88919-899-3
url https://www.frontiersin.org/research-topics/3172/optogenetic-tools-in-the-molecular-spotlight
http://hdl.handle.net/20.500.12010/14337
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.references.none.fl_str_mv Mathes, T., Kennis, J. T. M., eds. (2016). Optogenetic Tools in the Molecular Spotlight. Lausanne: Frontiers Media. doi: 10.3389/978-2-88919-899-3
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.local.spa.fl_str_mv Abierto (Texto Completo)
dc.rights.creativecommons.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv Abierto (Texto Completo)
https://creativecommons.org/licenses/by/4.0/
http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 181 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Frontiers Media SA
institution Universidad de Bogotá Jorge Tadeo Lozano
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https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/14337/3/license.txt
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spelling 2020-10-09T22:55:13Z2020-10-09T22:55:13Z2016-01-19978-2-889-19899-31664-8714https://www.frontiersin.org/research-topics/3172/optogenetic-tools-in-the-molecular-spotlighthttp://hdl.handle.net/20.500.12010/1433710.3389/978-2-88919-899-3181 páginasapplication/pdfengFrontiers Media SAScience (General)PhotoreceptorsFlavinsPhytochromePhotochemistry and photophysicsProtein engineeringComputational modelingOptogeneticsOpsinsSpectroscopyOptogenetic Tools in the Molecular SpotlightAbierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2Mathes, T., Kennis, J. T. M., eds. (2016). Optogenetic Tools in the Molecular Spotlight. Lausanne: Frontiers Media. doi: 10.3389/978-2-88919-899-3The rise of optogenetics as a standard technique to non-invasively probe and monitor biological function created an immense interest in the molecular function of photosensory proteins. These photoreceptors are usually protein/pigment complexes that translate light into biological information and have become essential tools in cell biology and neurobiology as their function is genetically encoded and can be conveniently delivered into a given cell. Like for fluorescent proteins that quickly became invaluable as genetically encodable reporters in microscopy and imaging, variants of photosensory proteins with customized sensitivity and functionality are nowadays in high demand. In this ebook we feature reviews and original research on molecular approaches from synthetic biology and molecular spectroscopy to computational molecular modelling that all aspire to elucidate the molecular prerequisites for the photosensory function of the given proteins. The principle property of changing activity of biological function simply by application of light is not only very attractive for cell biology, it also offers unique opportunities for molecular studies as excitation can be controlled with high time precision. Especially in spectroscopy the usually fully reversible photoactivation of photosensory proteins allows researchers to to perform time resolved studies with up to femtosecond resolution. In addition, functional variants can be investigated and quickly screened in common biochemical experiments. The insights that are obtained by the here presented various yet complementary methods will ultimately allow us write the script for a molecular movie from excitation of the protein by a photon to activation of its biological function. Such deep understanding does not only provide unique insights into the dynamics of protein function, it will also ultimately enable us to rationally design novel optogenetic tools to be used in cell biology and therapy.http://purl.org/coar/resource_type/c_2f33Mathes, TiloKennis, John T. 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