Successful use of buccal swabs to obtain genetic material from loggerhead sea turtles (caretta caretta).
Traditionally, the use of genetic material from sea turtles has been acquired from adult individuals employing invasive techniques like taking blood or skin tissue to obtain DNA. These methods tend to be expensive and time consuming and may harm or stress the individuals sampled, particularly neonat...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Bogotá Jorge Tadeo Lozano
- Repositorio:
- Expeditio: repositorio UTadeo
- Idioma:
- OAI Identifier:
- oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/9044
- Acceso en línea:
- https://www.researchgate.net/publication/318670816_Successful_Use_of_Buccal_Swabs_to_obtain_Genetic_Material_from_Loggerhead_Sea_Turtles_Caretta_caretta
http://hdl.handle.net/20.500.12010/9044
- Palabra clave:
- Genetic material
Sea turtles
Caretta caretta
Tortugas marinas -- Investigaciones
Genética animal -- Investigaciones
- Rights
- License
- Abierto (Texto Completo)
| Summary: | Traditionally, the use of genetic material from sea turtles has been acquired from adult individuals employing invasive techniques like taking blood or skin tissue to obtain DNA. These methods tend to be expensive and time consuming and may harm or stress the individuals sampled, particularly neonates and juveniles because blood is usually drawn from the dorsal cervical sinus in the neck or from the femoral venous plexus in the hind flipper (Wyneken 2001; Wallace & George 2007), while skin is sampled using a razor blade or biopsy punch to collect tissue from the top few layers of the turtle’s epidermis (Dutton & Balazs 1995; Dutton 1996). Both methods, particularly blood sampling, require samplehandling protocols that may not be feasible under challenging field conditions or they may not be permitted by regulatory agencies. |
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