Mini Review: Recent progress in RT-LAMP enabled COVID-19 detection
The coronavirus disease 2019 (COVID-19) pandemic has infected millions of people around the globe. The outbreak caused by the novel coronavirus (SARS-CoV-2) poses a great health risk to the public. Therefore, rapid and accurate diagnosis of the virus plays a crucial role in treatment of the disease...
- Autores:
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2020
- Institución:
- Universidad de Bogotá Jorge Tadeo Lozano
- Repositorio:
- Expeditio: repositorio UTadeo
- Idioma:
- eng
- OAI Identifier:
- oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/12296
- Palabra clave:
- RT-LAMP
COVID-19
Molecular diagnosis
Isothermal amplification
Rapid
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
- Rights
- License
- Abierto (Texto Completo)
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UTADEO2 |
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|
dc.title.spa.fl_str_mv |
Mini Review: Recent progress in RT-LAMP enabled COVID-19 detection |
title |
Mini Review: Recent progress in RT-LAMP enabled COVID-19 detection |
spellingShingle |
Mini Review: Recent progress in RT-LAMP enabled COVID-19 detection RT-LAMP COVID-19 Molecular diagnosis Isothermal amplification Rapid Síndrome respiratorio agudo grave COVID-19 SARS-CoV-2 Coronavirus |
title_short |
Mini Review: Recent progress in RT-LAMP enabled COVID-19 detection |
title_full |
Mini Review: Recent progress in RT-LAMP enabled COVID-19 detection |
title_fullStr |
Mini Review: Recent progress in RT-LAMP enabled COVID-19 detection |
title_full_unstemmed |
Mini Review: Recent progress in RT-LAMP enabled COVID-19 detection |
title_sort |
Mini Review: Recent progress in RT-LAMP enabled COVID-19 detection |
dc.subject.spa.fl_str_mv |
RT-LAMP COVID-19 Molecular diagnosis Isothermal amplification Rapid |
topic |
RT-LAMP COVID-19 Molecular diagnosis Isothermal amplification Rapid Síndrome respiratorio agudo grave COVID-19 SARS-CoV-2 Coronavirus |
dc.subject.lemb.spa.fl_str_mv |
Síndrome respiratorio agudo grave COVID-19 SARS-CoV-2 Coronavirus |
description |
The coronavirus disease 2019 (COVID-19) pandemic has infected millions of people around the globe. The outbreak caused by the novel coronavirus (SARS-CoV-2) poses a great health risk to the public. Therefore, rapid and accurate diagnosis of the virus plays a crucial role in treatment of the disease and saving lives. The current standard method for coronavirus detection is the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. However, laboratorybased RT-PCR test for SARS-COV-2 requires complex facilities and elaborate training of operators, thus suffering from limit testing capacity and delayed results. Consequently, isothermal PCR such as Loop-mediated isothermal amplification (LAMP) has been emerging as a great alternative to the RT-PCR method. LAMP possesses some fundamental advantages such as amplification at a constant temperature, no need of a thermal cycler, a faster test result, and potentially a larger diagnostic capacity, while maintaining similar sensitivity and specificity, thus making it more suitable than the RT-PCR for monitoring a pandemic. Starting with a brief introduction of the working principle of LAMP method, this review summarizes recent progress in LAMP-enabled SARS-CoV-2 viral RNA detection. Lastly, future research directions are discussed. This critical review will motivate biosensor community in furthering the present research, which may pave the road for rapid and large-scale screening of SARS-CoV-2. |
publishDate |
2020 |
dc.date.accessioned.none.fl_str_mv |
2020-08-26T14:10:57Z |
dc.date.available.none.fl_str_mv |
2020-08-26T14:10:57Z |
dc.date.created.none.fl_str_mv |
2020 |
dc.type.local.spa.fl_str_mv |
Artículo |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.identifier.other.spa.fl_str_mv |
https://doi.org/10.1016/j.snr.2020.100017 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/20.500.12010/12296 |
dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.1016/j.snr.2020.100017 |
url |
https://doi.org/10.1016/j.snr.2020.100017 http://hdl.handle.net/20.500.12010/12296 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.local.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.extent.spa.fl_str_mv |
32 páginas |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Sensors and Actuators Reports |
dc.source.spa.fl_str_mv |
reponame:Expeditio Repositorio Institucional UJTL instname:Universidad de Bogotá Jorge Tadeo Lozano |
instname_str |
Universidad de Bogotá Jorge Tadeo Lozano |
institution |
Universidad de Bogotá Jorge Tadeo Lozano |
reponame_str |
Expeditio Repositorio Institucional UJTL |
collection |
Expeditio Repositorio Institucional UJTL |
bitstream.url.fl_str_mv |
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bitstream.checksum.fl_str_mv |
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bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional - Universidad Jorge Tadeo Lozano |
repository.mail.fl_str_mv |
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1818152904806105088 |
spelling |
2020-08-26T14:10:57Z2020-08-26T14:10:57Z2020https://doi.org/10.1016/j.snr.2020.100017http://hdl.handle.net/20.500.12010/12296https://doi.org/10.1016/j.snr.2020.100017The coronavirus disease 2019 (COVID-19) pandemic has infected millions of people around the globe. The outbreak caused by the novel coronavirus (SARS-CoV-2) poses a great health risk to the public. Therefore, rapid and accurate diagnosis of the virus plays a crucial role in treatment of the disease and saving lives. The current standard method for coronavirus detection is the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. However, laboratorybased RT-PCR test for SARS-COV-2 requires complex facilities and elaborate training of operators, thus suffering from limit testing capacity and delayed results. Consequently, isothermal PCR such as Loop-mediated isothermal amplification (LAMP) has been emerging as a great alternative to the RT-PCR method. LAMP possesses some fundamental advantages such as amplification at a constant temperature, no need of a thermal cycler, a faster test result, and potentially a larger diagnostic capacity, while maintaining similar sensitivity and specificity, thus making it more suitable than the RT-PCR for monitoring a pandemic. Starting with a brief introduction of the working principle of LAMP method, this review summarizes recent progress in LAMP-enabled SARS-CoV-2 viral RNA detection. Lastly, future research directions are discussed. This critical review will motivate biosensor community in furthering the present research, which may pave the road for rapid and large-scale screening of SARS-CoV-2.32 páginasapplication/pdfengSensors and Actuators Reportsreponame:Expeditio Repositorio Institucional UJTLinstname:Universidad de Bogotá Jorge Tadeo LozanoRT-LAMPCOVID-19Molecular diagnosisIsothermal amplificationRapidSíndrome respiratorio agudo graveCOVID-19SARS-CoV-2CoronavirusMini Review: Recent progress in RT-LAMP enabled COVID-19 detectionArtículohttp://purl.org/coar/resource_type/c_2df8fbb1Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Thompson, DorianLei, YuORIGINAL1-s2.0-S266605392030014X-main.pdf1-s2.0-S266605392030014X-main.pdfVer artículoapplication/pdf1329688https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12296/1/1-s2.0-S266605392030014X-main.pdf7147a01d52624649552b70e7bbe4e38eMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-82938https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12296/2/license.txtabceeb1c943c50d3343516f9dbfc110fMD52open accessTHUMBNAIL1-s2.0-S266605392030014X-main.pdf.jpg1-s2.0-S266605392030014X-main.pdf.jpgIM Thumbnailimage/jpeg9852https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12296/3/1-s2.0-S266605392030014X-main.pdf.jpg0c4c4ef68cf091c460139606a5b60578MD53open access20.500.12010/12296oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/122962020-08-26 09:10:58.093open accessRepositorio Institucional - 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