Single-cell transcriptomic atlas of primate cardiopulmonary aging

Aging is a major risk factor for many diseases, especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Disease 2019 (COVID-19). Resolving cellular and molecular mechanisms associated with aging in higher mammals is therefore urgently needed. Here,...

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Autores:
Tipo de recurso:
Article of journal
Fecha de publicación:
2020
Institución:
Universidad de Bogotá Jorge Tadeo Lozano
Repositorio:
Expeditio: repositorio UTadeo
Idioma:
eng
OAI Identifier:
oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/13458
Acceso en línea:
https://www.nature.com/articles/s41422-020-00412-6
http://hdl.handle.net/20.500.12010/13458
https://doi.org/10.1038/s41422-020-00412-6
Palabra clave:
Single-cell transcriptomic atlas
primate cardiopulmonary aging
cardiopulmonary
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
Rights
License
Acceso restringido
id UTADEO2_4b519210947a00502b521c80c1ba9c31
oai_identifier_str oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/13458
network_acronym_str UTADEO2
network_name_str Expeditio: repositorio UTadeo
repository_id_str
dc.title.spa.fl_str_mv Single-cell transcriptomic atlas of primate cardiopulmonary aging
title Single-cell transcriptomic atlas of primate cardiopulmonary aging
spellingShingle Single-cell transcriptomic atlas of primate cardiopulmonary aging
Single-cell transcriptomic atlas
primate cardiopulmonary aging
cardiopulmonary
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
title_short Single-cell transcriptomic atlas of primate cardiopulmonary aging
title_full Single-cell transcriptomic atlas of primate cardiopulmonary aging
title_fullStr Single-cell transcriptomic atlas of primate cardiopulmonary aging
title_full_unstemmed Single-cell transcriptomic atlas of primate cardiopulmonary aging
title_sort Single-cell transcriptomic atlas of primate cardiopulmonary aging
dc.subject.spa.fl_str_mv Single-cell transcriptomic atlas
primate cardiopulmonary aging
cardiopulmonary
topic Single-cell transcriptomic atlas
primate cardiopulmonary aging
cardiopulmonary
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
dc.subject.lemb.spa.fl_str_mv Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
description Aging is a major risk factor for many diseases, especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Disease 2019 (COVID-19). Resolving cellular and molecular mechanisms associated with aging in higher mammals is therefore urgently needed. Here, we created young and old non-human primate single-nucleus/cell transcriptomic atlases of lung, heart and artery, the top tissues targeted by SARS-CoV-2. Analysis of cell type-specific aging-associated transcriptional changes revealed increased systemic inflammation and compromised virus defense as a hallmark of cardiopulmonary aging. With age, expression of the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) was increased in the pulmonary alveolar epithelial barrier, cardiomyocytes, and vascular endothelial cells. We found that interleukin 7 (IL7) accumulated in aged cardiopulmonary tissues and induced ACE2 expression in human vascular endothelial cells in an NF-κB-dependent manner. Furthermore, treatment with vitamin C blocked IL7-induced ACE2 expression. Altogether, our findings depict the first transcriptomic atlas of the aged primate cardiopulmonary system and provide vital insights into age-linked susceptibility to SARS-CoV-2, suggesting that geroprotective strategies may reduce COVID-19 severity in the elderly.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-09-18T16:28:52Z
dc.date.available.none.fl_str_mv 2020-09-18T16:28:52Z
dc.date.created.none.fl_str_mv 2020-06-29
dc.type.local.spa.fl_str_mv Artículo
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
format http://purl.org/coar/resource_type/c_6501
dc.identifier.issn.spa.fl_str_mv 1748-7838
dc.identifier.other.spa.fl_str_mv https://www.nature.com/articles/s41422-020-00412-6
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12010/13458
dc.identifier.doi.spa.fl_str_mv https://doi.org/10.1038/s41422-020-00412-6
identifier_str_mv 1748-7838
url https://www.nature.com/articles/s41422-020-00412-6
http://hdl.handle.net/20.500.12010/13458
https://doi.org/10.1038/s41422-020-00412-6
dc.language.iso.spa.fl_str_mv eng
language eng
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.local.spa.fl_str_mv Acceso restringido
rights_invalid_str_mv Acceso restringido
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dc.format.extent.spa.fl_str_mv 18 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Cell Research
dc.source.spa.fl_str_mv reponame:Expeditio Repositorio Institucional UJTL
instname:Universidad de Bogotá Jorge Tadeo Lozano
instname_str Universidad de Bogotá Jorge Tadeo Lozano
institution Universidad de Bogotá Jorge Tadeo Lozano
reponame_str Expeditio Repositorio Institucional UJTL
collection Expeditio Repositorio Institucional UJTL
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https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/13458/3/Single-cell%20transcriptomic%20atlas%20of%20primate%20cardiopulmonary%20aging.pdf
https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/13458/2/license.txt
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spelling 2020-09-18T16:28:52Z2020-09-18T16:28:52Z2020-06-291748-7838https://www.nature.com/articles/s41422-020-00412-6http://hdl.handle.net/20.500.12010/13458https://doi.org/10.1038/s41422-020-00412-618 páginasapplication/pdfengCell Researchreponame:Expeditio Repositorio Institucional UJTLinstname:Universidad de Bogotá Jorge Tadeo LozanoSingle-cell transcriptomic atlasprimate cardiopulmonary agingcardiopulmonarySíndrome respiratorio agudo graveCOVID-19SARS-CoV-2CoronavirusSingle-cell transcriptomic atlas of primate cardiopulmonary agingArtículohttp://purl.org/coar/resource_type/c_6501Acceso restringidohttp://purl.org/coar/access_right/c_16ecAging is a major risk factor for many diseases, especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Disease 2019 (COVID-19). Resolving cellular and molecular mechanisms associated with aging in higher mammals is therefore urgently needed. Here, we created young and old non-human primate single-nucleus/cell transcriptomic atlases of lung, heart and artery, the top tissues targeted by SARS-CoV-2. Analysis of cell type-specific aging-associated transcriptional changes revealed increased systemic inflammation and compromised virus defense as a hallmark of cardiopulmonary aging. With age, expression of the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) was increased in the pulmonary alveolar epithelial barrier, cardiomyocytes, and vascular endothelial cells. We found that interleukin 7 (IL7) accumulated in aged cardiopulmonary tissues and induced ACE2 expression in human vascular endothelial cells in an NF-κB-dependent manner. Furthermore, treatment with vitamin C blocked IL7-induced ACE2 expression. Altogether, our findings depict the first transcriptomic atlas of the aged primate cardiopulmonary system and provide vital insights into age-linked susceptibility to SARS-CoV-2, suggesting that geroprotective strategies may reduce COVID-19 severity in the elderly.Ma, ShuaiSun, ShuhuiLi, JiamingFan, YanlingQu, JingSun, LiangWang, SiZhang, YiyuanYang, ShanshanLiu, ZunpengWu, ZemingZhang, ShengWang, QiaoranZheng, AihuaDuo, ShuguangYu, YangIzpisua Belmonte, Juan CarlosChan, PiuZhou, QiSong, MoshiZhang, WeiqiLiu, Guang-HuiTHUMBNAILSingle-cell transcriptomic atlas of primate cardiopulmonary aging.pngSingle-cell transcriptomic atlas of primate cardiopulmonary aging.pngimage/png98453https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/13458/4/Single-cell%20transcriptomic%20atlas%20of%20primate%20cardiopulmonary%20aging.png1e0afdeb3eb5761b0f492e0888733bd8MD54open accessSingle-cell transcriptomic atlas of primate cardiopulmonary aging.pdf.jpgSingle-cell transcriptomic atlas of primate cardiopulmonary aging.pdf.jpgIM Thumbnailimage/jpeg23488https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/13458/5/Single-cell%20transcriptomic%20atlas%20of%20primate%20cardiopulmonary%20aging.pdf.jpg112a146c4e0e3fb574818d7ee02e3182MD55open accessORIGINALSingle-cell transcriptomic atlas of primate cardiopulmonary aging.pdfSingle-cell transcriptomic atlas of primate cardiopulmonary aging.pdfDocumento Reservadoapplication/pdf7015024https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/13458/3/Single-cell%20transcriptomic%20atlas%20of%20primate%20cardiopulmonary%20aging.pdfcdc904e7d5cd68d83e386d7b40936e28MD53embargoed access|||2420-09-18LICENSElicense.txtlicense.txttext/plain; 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