Clinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19

Background We evaluated the clinical performance of an immunochromatographic (IC) IgM/IgG antibody assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) and chest computed tomography (CT) for the diagnosis of Coronavirus disease 2019 (COVID-19). Methods We examined 139 serum specimen...

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Tipo de recurso:
Article of journal
Fecha de publicación:
2020
Institución:
Universidad de Bogotá Jorge Tadeo Lozano
Repositorio:
Expeditio: repositorio UTadeo
Idioma:
eng
OAI Identifier:
oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/12268
Acceso en línea:
https://www.sciencedirect.com/science/article/pii/S1386653220301359?via%3Dihub
http://hdl.handle.net/20.500.12010/12268
https://doi.org/10.1016/j.jcv.2020.104393
Palabra clave:
COVID-19
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
Immunochromatographic assay
Chest CT scan
Rights
License
Acceso restringido
id UTADEO2_4a1f86cd3013517d35c5128569620ad2
oai_identifier_str oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/12268
network_acronym_str UTADEO2
network_name_str Expeditio: repositorio UTadeo
repository_id_str
dc.title.spa.fl_str_mv Clinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19
title Clinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19
spellingShingle Clinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19
COVID-19
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
Immunochromatographic assay
Chest CT scan
title_short Clinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19
title_full Clinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19
title_fullStr Clinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19
title_full_unstemmed Clinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19
title_sort Clinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19
dc.subject.spa.fl_str_mv COVID-19
topic COVID-19
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
Immunochromatographic assay
Chest CT scan
dc.subject.lemb.spa.fl_str_mv Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
dc.subject.keyword.spa.fl_str_mv Immunochromatographic assay
Chest CT scan
description Background We evaluated the clinical performance of an immunochromatographic (IC) IgM/IgG antibody assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) and chest computed tomography (CT) for the diagnosis of Coronavirus disease 2019 (COVID-19). Methods We examined 139 serum specimens collected from 112 patients with COVID-19 and 48 serum specimens collected from 48 non-COVID-19 patients. The presence of IgM/IgG antibody for SARS-COV2 was determined using the One Step Novel Coronavirus (COVID-19) IgM/IgG Antibody Test. Chest CT was performed in COVID-19 patients on admission. Findings Of the139 COVID-19 serum specimens, IgM was detected in 27.8 %, 48.0 %, and 95.8 % of the specimens collected within 1 week, 1–2 weeks, and >2 weeks after symptom onset and IgG was detected in 3.3 %, 8.0 %, and 62.5 %, respectively. Among the 48 non-COVID-19 serum specimens, 1 generated a false-positive result for IgM. Thirty-eight of the 112 COVID-19 patients were asymptomatic, of whom 15 were positive for IgM, and 74 were symptomatic, of whom 22 were positive for IgM and 7 were positive for IgG. The diagnostic sensitivity of CT scan alone and in combination with the IC assay was 57.9 % (22/38) and 68.4 % (26/38) for the asymptomatic patients and 74.3 % (55/74) and 82.4 % (61/74) for the symptomatic patients, respectively. Conclusion The IC assay had low sensitivity during the early phase of infection, and thus IC assay alone is not recommended for initial diagnostic testing for COVID-19. If RT-qPCR is not available, the combination of chest CT and IC assay may be useful for diagnosing COVID-19.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-08-25T22:43:29Z
dc.date.available.none.fl_str_mv 2020-08-25T22:43:29Z
dc.date.created.none.fl_str_mv 2020-07
dc.type.local.spa.fl_str_mv Artículo
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
format http://purl.org/coar/resource_type/c_6501
dc.identifier.issn.spa.fl_str_mv 1386-6532
dc.identifier.other.spa.fl_str_mv https://www.sciencedirect.com/science/article/pii/S1386653220301359?via%3Dihub
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12010/12268
dc.identifier.doi.spa.fl_str_mv https://doi.org/10.1016/j.jcv.2020.104393
identifier_str_mv 1386-6532
url https://www.sciencedirect.com/science/article/pii/S1386653220301359?via%3Dihub
http://hdl.handle.net/20.500.12010/12268
https://doi.org/10.1016/j.jcv.2020.104393
dc.language.iso.spa.fl_str_mv eng
language eng
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_f1cf
dc.rights.local.spa.fl_str_mv Acceso restringido
rights_invalid_str_mv Acceso restringido
http://purl.org/coar/access_right/c_f1cf
dc.format.extent.spa.fl_str_mv 4 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Journal of Clinical Virology
dc.source.spa.fl_str_mv reponame:Expeditio Repositorio Institucional UJTL
instname:Universidad de Bogotá Jorge Tadeo Lozano
instname_str Universidad de Bogotá Jorge Tadeo Lozano
institution Universidad de Bogotá Jorge Tadeo Lozano
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https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12268/2/license.txt
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spelling 2020-08-25T22:43:29Z2020-08-25T22:43:29Z2020-071386-6532https://www.sciencedirect.com/science/article/pii/S1386653220301359?via%3Dihubhttp://hdl.handle.net/20.500.12010/12268https://doi.org/10.1016/j.jcv.2020.1043934 páginasapplication/pdfengJournal of Clinical Virologyreponame:Expeditio Repositorio Institucional UJTLinstname:Universidad de Bogotá Jorge Tadeo LozanoCOVID-19Síndrome respiratorio agudo graveCOVID-19SARS-CoV-2CoronavirusImmunochromatographic assayChest CT scanClinical evaluation of an immunochromatographic IgM/IgG antibody assay and chest computed tomography for the diagnosis of COVID-19Artículohttp://purl.org/coar/resource_type/c_6501Acceso restringidohttp://purl.org/coar/access_right/c_f1cfBackground We evaluated the clinical performance of an immunochromatographic (IC) IgM/IgG antibody assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) and chest computed tomography (CT) for the diagnosis of Coronavirus disease 2019 (COVID-19). Methods We examined 139 serum specimens collected from 112 patients with COVID-19 and 48 serum specimens collected from 48 non-COVID-19 patients. The presence of IgM/IgG antibody for SARS-COV2 was determined using the One Step Novel Coronavirus (COVID-19) IgM/IgG Antibody Test. Chest CT was performed in COVID-19 patients on admission. Findings Of the139 COVID-19 serum specimens, IgM was detected in 27.8 %, 48.0 %, and 95.8 % of the specimens collected within 1 week, 1–2 weeks, and >2 weeks after symptom onset and IgG was detected in 3.3 %, 8.0 %, and 62.5 %, respectively. Among the 48 non-COVID-19 serum specimens, 1 generated a false-positive result for IgM. Thirty-eight of the 112 COVID-19 patients were asymptomatic, of whom 15 were positive for IgM, and 74 were symptomatic, of whom 22 were positive for IgM and 7 were positive for IgG. The diagnostic sensitivity of CT scan alone and in combination with the IC assay was 57.9 % (22/38) and 68.4 % (26/38) for the asymptomatic patients and 74.3 % (55/74) and 82.4 % (61/74) for the symptomatic patients, respectively. Conclusion The IC assay had low sensitivity during the early phase of infection, and thus IC assay alone is not recommended for initial diagnostic testing for COVID-19. If RT-qPCR is not available, the combination of chest CT and IC assay may be useful for diagnosing COVID-19.Imai, KazuoTabata, SakikoIkeda, MayuNoguchi, SakikoKitagawa, YutaroMatuoka, MasaruMiyoshi, KazuyasuTarumoto, NorihitoSakai, JunIto, ToshimitsuMaesaki, ShigefumiTamura, KakuMaeda, TakuyaTHUMBNAILCaptura.JPGCaptura.JPGimage/jpeg68152https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12268/4/Captura.JPGf5ebba8ddafdcdf3296b647e5afbc083MD54open accessClinical-evaluation-of-an-immunochromatographic-IgM-IgG-an_2020_Journal-of-C.pdf.jpgClinical-evaluation-of-an-immunochromatographic-IgM-IgG-an_2020_Journal-of-C.pdf.jpgIM Thumbnailimage/jpeg21085https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12268/5/Clinical-evaluation-of-an-immunochromatographic-IgM-IgG-an_2020_Journal-of-C.pdf.jpg55f57b64ab6362f411a2c8ed93113082MD55open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-82938https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12268/2/license.txtabceeb1c943c50d3343516f9dbfc110fMD52open accessORIGINALClinical-evaluation-of-an-immunochromatographic-IgM-IgG-an_2020_Journal-of-C.pdfClinical-evaluation-of-an-immunochromatographic-IgM-IgG-an_2020_Journal-of-C.pdfDocumento reservadoapplication/pdf489025https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12268/3/Clinical-evaluation-of-an-immunochromatographic-IgM-IgG-an_2020_Journal-of-C.pdfa5ce698440b179d52d04441e0829bf38MD53embargoed access|||2220-08-2520.500.12010/12268oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/122682020-08-25 17:48:35.392embargoed access|||2220-08-25Repositorio Institucional - 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