Common-path interferometric label-free protein sensing with resonant dielectric nanostructures

Research toward photonic biosensors for point-of-care applications and personalized medicine is driven by the need for high-sensitivity, low-cost, and reliable technology. Among the most sensitive modalities, interferometry offers particularly high performance, but typically lacks the required opera...

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Autores:
Tipo de recurso:
Article of journal
Fecha de publicación:
2020
Institución:
Universidad de Bogotá Jorge Tadeo Lozano
Repositorio:
Expeditio: repositorio UTadeo
Idioma:
eng
OAI Identifier:
oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/12891
Acceso en línea:
https://www.nature.com/articles/s41377-020-0336-6
http://hdl.handle.net/20.500.12010/12891
https://doi.org/10.1038/s41377-020-0336-6
Palabra clave:
resonant dielectric nanostructures
Common-path interferometric label-free protein
protein
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
Rights
License
Acceso restringido
id UTADEO2_2bd94d731f17e5df43adf6253458735c
oai_identifier_str oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/12891
network_acronym_str UTADEO2
network_name_str Expeditio: repositorio UTadeo
repository_id_str
dc.title.spa.fl_str_mv Common-path interferometric label-free protein sensing with resonant dielectric nanostructures
title Common-path interferometric label-free protein sensing with resonant dielectric nanostructures
spellingShingle Common-path interferometric label-free protein sensing with resonant dielectric nanostructures
resonant dielectric nanostructures
Common-path interferometric label-free protein
protein
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
title_short Common-path interferometric label-free protein sensing with resonant dielectric nanostructures
title_full Common-path interferometric label-free protein sensing with resonant dielectric nanostructures
title_fullStr Common-path interferometric label-free protein sensing with resonant dielectric nanostructures
title_full_unstemmed Common-path interferometric label-free protein sensing with resonant dielectric nanostructures
title_sort Common-path interferometric label-free protein sensing with resonant dielectric nanostructures
dc.subject.spa.fl_str_mv resonant dielectric nanostructures
Common-path interferometric label-free protein
protein
topic resonant dielectric nanostructures
Common-path interferometric label-free protein
protein
Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
dc.subject.lemb.spa.fl_str_mv Síndrome respiratorio agudo grave
COVID-19
SARS-CoV-2
Coronavirus
description Research toward photonic biosensors for point-of-care applications and personalized medicine is driven by the need for high-sensitivity, low-cost, and reliable technology. Among the most sensitive modalities, interferometry offers particularly high performance, but typically lacks the required operational simplicity and robustness. Here, we introduce a common-path interferometric sensor based on guided-mode resonances to combine high performance with inherent stability. The sensor exploits the simultaneous excitation of two orthogonally polarized modes, and detects the relative phase change caused by biomolecular binding on the sensor surface. The wide dynamic range of the sensor, which is essential for fabrication and angle tolerance, as well as versatility, is controlled by integrating multiple, tuned structures in the field of view. This approach circumvents the trade-off between sensitivity and dynamic range, typical of other phase-sensitive modalities, without increasing complexity. Our sensor enables the challenging label-free detection of procalcitonin, a small protein (13 kDa) and biomarker for infection, at the clinically relevant concentration of 1 pg mL−1, with a signal-to-noise ratio of 35. This result indicates the utility for an exemplary application in antibiotic guidance, and opens possibilities for detecting further clinically or environmentally relevant small molecules with an intrinsically simple and robust sensing modality.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-09-08T13:26:49Z
dc.date.available.none.fl_str_mv 2020-09-08T13:26:49Z
dc.date.created.none.fl_str_mv 2020-06-02
dc.type.local.spa.fl_str_mv Artículo
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
format http://purl.org/coar/resource_type/c_6501
dc.identifier.issn.spa.fl_str_mv 2047-7538
dc.identifier.other.spa.fl_str_mv https://www.nature.com/articles/s41377-020-0336-6
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/20.500.12010/12891
dc.identifier.doi.spa.fl_str_mv https://doi.org/10.1038/s41377-020-0336-6
identifier_str_mv 2047-7538
url https://www.nature.com/articles/s41377-020-0336-6
http://hdl.handle.net/20.500.12010/12891
https://doi.org/10.1038/s41377-020-0336-6
dc.language.iso.spa.fl_str_mv eng
language eng
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.local.spa.fl_str_mv Acceso restringido
rights_invalid_str_mv Acceso restringido
http://purl.org/coar/access_right/c_16ec
dc.format.extent.spa.fl_str_mv 9 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv light: science & applications
dc.source.spa.fl_str_mv reponame:Expeditio Repositorio Institucional UJTL
instname:Universidad de Bogotá Jorge Tadeo Lozano
instname_str Universidad de Bogotá Jorge Tadeo Lozano
institution Universidad de Bogotá Jorge Tadeo Lozano
reponame_str Expeditio Repositorio Institucional UJTL
collection Expeditio Repositorio Institucional UJTL
bitstream.url.fl_str_mv https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12891/3/Common-path%20interferometric%20label-free%20protein%20sensing%20with%20resonant%20dielectric%20nanostructures.pdf
https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12891/2/license.txt
https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12891/4/Common-path%20interferometric%20label-free%20protein%20sensing%20with%20resonant%20dielectric%20nanostructures.png
https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12891/5/Common-path%20interferometric%20label-free%20protein%20sensing%20with%20resonant%20dielectric%20nanostructures.pdf.jpg
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spelling 2020-09-08T13:26:49Z2020-09-08T13:26:49Z2020-06-022047-7538https://www.nature.com/articles/s41377-020-0336-6http://hdl.handle.net/20.500.12010/12891https://doi.org/10.1038/s41377-020-0336-69 páginasapplication/pdfenglight: science & applicationsreponame:Expeditio Repositorio Institucional UJTLinstname:Universidad de Bogotá Jorge Tadeo Lozanoresonant dielectric nanostructuresCommon-path interferometric label-free proteinproteinSíndrome respiratorio agudo graveCOVID-19SARS-CoV-2CoronavirusCommon-path interferometric label-free protein sensing with resonant dielectric nanostructuresArtículohttp://purl.org/coar/resource_type/c_6501Acceso restringidohttp://purl.org/coar/access_right/c_16ecResearch toward photonic biosensors for point-of-care applications and personalized medicine is driven by the need for high-sensitivity, low-cost, and reliable technology. Among the most sensitive modalities, interferometry offers particularly high performance, but typically lacks the required operational simplicity and robustness. Here, we introduce a common-path interferometric sensor based on guided-mode resonances to combine high performance with inherent stability. The sensor exploits the simultaneous excitation of two orthogonally polarized modes, and detects the relative phase change caused by biomolecular binding on the sensor surface. The wide dynamic range of the sensor, which is essential for fabrication and angle tolerance, as well as versatility, is controlled by integrating multiple, tuned structures in the field of view. This approach circumvents the trade-off between sensitivity and dynamic range, typical of other phase-sensitive modalities, without increasing complexity. Our sensor enables the challenging label-free detection of procalcitonin, a small protein (13 kDa) and biomarker for infection, at the clinically relevant concentration of 1 pg mL−1, with a signal-to-noise ratio of 35. This result indicates the utility for an exemplary application in antibiotic guidance, and opens possibilities for detecting further clinically or environmentally relevant small molecules with an intrinsically simple and robust sensing modality.Barth, IsabelConteduca, DonatoReardon, ChristopherJohnson, StevenKrauss, Thomas F.ORIGINALCommon-path interferometric label-free protein sensing with resonant dielectric nanostructures.pdfCommon-path interferometric label-free protein sensing with resonant dielectric nanostructures.pdfDocumento Reservadoapplication/pdf1203084https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12891/3/Common-path%20interferometric%20label-free%20protein%20sensing%20with%20resonant%20dielectric%20nanostructures.pdfd81de79a1e27f085bd2431ad85fe96ffMD53embargoed access|||2420-09-08LICENSElicense.txtlicense.txttext/plain; charset=utf-82938https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12891/2/license.txtabceeb1c943c50d3343516f9dbfc110fMD52open accessTHUMBNAILCommon-path interferometric label-free protein sensing with resonant dielectric nanostructures.pngCommon-path interferometric label-free protein sensing with resonant dielectric nanostructures.pngimage/png92406https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12891/4/Common-path%20interferometric%20label-free%20protein%20sensing%20with%20resonant%20dielectric%20nanostructures.png007939829a19cb45b382b43a498e698fMD54open accessCommon-path interferometric label-free protein sensing with resonant dielectric nanostructures.pdf.jpgCommon-path interferometric label-free protein sensing with resonant dielectric nanostructures.pdf.jpgIM Thumbnailimage/jpeg22949https://expeditiorepositorio.utadeo.edu.co/bitstream/20.500.12010/12891/5/Common-path%20interferometric%20label-free%20protein%20sensing%20with%20resonant%20dielectric%20nanostructures.pdf.jpg058d1e836ec2866cdc3c21da210d1f2dMD55open access20.500.12010/12891oai:expeditiorepositorio.utadeo.edu.co:20.500.12010/128912020-09-08 08:30:12.042embargoed access|||2420-09-08Repositorio Institucional - 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