Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica

Exploration of the e ect of soil bacteria on growth and metabolism of beneficial root endophytic fungi is relevant to promote favorable associations between microorganisms of the plant rhizosphere. Hence, the interaction between the plant-growth-promoting fungus Piriformospora indica and di erent so...

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Autores:: Leyva-Rojas, Jorge A.
Coy-Barrera, Ericsson
Hampp, Rüdiger

Tipo de recurso:

Fecha de publicación:: 2020

Institución:: Universidad Simón Bolívar

Repositorio:: Repositorio Digital USB

Idioma:: eng

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dc.title.eng.fl_str_mv	Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica
title	Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica
spellingShingle	Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica Piriformospora indica Endophytic fungi Mycorrhiza helper bacteria Amino acid content
title_short	Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica
title_full	Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica
title_fullStr	Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica
title_full_unstemmed	Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica
title_sort	Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica
dc.creator.fl_str_mv	Leyva-Rojas, Jorge A. Coy-Barrera, Ericsson Hampp, Rüdiger
dc.contributor.author.none.fl_str_mv	Leyva-Rojas, Jorge A. Coy-Barrera, Ericsson Hampp, Rüdiger
dc.subject.eng.fl_str_mv	Piriformospora indica Endophytic fungi Mycorrhiza helper bacteria Amino acid content
topic	Piriformospora indica Endophytic fungi Mycorrhiza helper bacteria Amino acid content
description	Exploration of the e ect of soil bacteria on growth and metabolism of beneficial root endophytic fungi is relevant to promote favorable associations between microorganisms of the plant rhizosphere. Hence, the interaction between the plant-growth-promoting fungus Piriformospora indica and di erent soil bacteria was investigated. The parameters studied were fungal growth and its amino acid composition during the interaction. Fungus and bacteria were confronted in dual cultures in Petri dishes, either through agar or separated by a Perspex wall that only allowed the bacterial volatiles to be e ective. Fungal growth was stimulated by Azotobacter chroococcum, whereas Streptomyces anulatus AcH 1003 inhibited it and Streptomyces sp. Nov AcH 505 had no e ect. To analyze amino acid concentration data, targeted metabolomics was implemented under supervised analysis according to fungal-bacteria interaction and time. Orthogonal partial least squares-discriminant analysis (OPLS-DA) model clearly discriminated P. indica–A. chroococcum and P. indica–S. anulatus interactions, according to the respective score plot in comparison to the control. The most observable responses were in the glutamine and alanine size groups: While Streptomyces AcH 1003 increased the amount of glutamine, A. chroococcum decreased it. The fungal growth and the increase of alanine content might be associated with the assimilation of nitrogen in the presence of glucose as a carbon source. The N-fixing bacterium A. chroococcum should stimulate fungal amino acid metabolism via glutamine synthetase-glutamate synthase (GS-GOGAT). The data pointed to a stimulated glycolytic activity in the fungus observed by the accumulation of alanine, possibly via alanine aminotransferase. The responses toward the growth-inhibiting Streptomyces AcH 1003 suggest an (oxidative) stress response of the fungus.
publishDate	2020
dc.date.accessioned.none.fl_str_mv	2020-02-10T23:00:13Z
dc.date.available.none.fl_str_mv	2020-02-10T23:00:13Z
dc.date.issued.none.fl_str_mv	2020-01
dc.type.eng.fl_str_mv	article
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dc.type.driver.eng.fl_str_mv	article
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12442/4715
url	https://hdl.handle.net/20.500.12442/4715
dc.language.iso.eng.fl_str_mv	eng
language	eng
dc.rights.*.fl_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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dc.format.mimetype.spa.fl_str_mv	pdf
dc.publisher.eng.fl_str_mv	MDPI
dc.source.eng.fl_str_mv	Molecules
dc.source.spa.fl_str_mv	Vol. 25, No. 3 (2020)
institution	Universidad Simón Bolívar
dc.source.uri.eng.fl_str_mv	https://doi.org/10.3390/molecules25030572
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spelling	Leyva-Rojas, Jorge A.6c62ccf2-5642-4dc8-b32a-1a042526f75eCoy-Barrera, Ericsson81b6d0b7-0ff3-4df7-833f-7f955508f90dHampp, Rüdigeread459f0-0624-4885-8fef-dae93fa887682020-02-10T23:00:13Z2020-02-10T23:00:13Z2020-01https://hdl.handle.net/20.500.12442/4715Exploration of the e ect of soil bacteria on growth and metabolism of beneficial root endophytic fungi is relevant to promote favorable associations between microorganisms of the plant rhizosphere. Hence, the interaction between the plant-growth-promoting fungus Piriformospora indica and di erent soil bacteria was investigated. The parameters studied were fungal growth and its amino acid composition during the interaction. Fungus and bacteria were confronted in dual cultures in Petri dishes, either through agar or separated by a Perspex wall that only allowed the bacterial volatiles to be e ective. Fungal growth was stimulated by Azotobacter chroococcum, whereas Streptomyces anulatus AcH 1003 inhibited it and Streptomyces sp. Nov AcH 505 had no e ect. To analyze amino acid concentration data, targeted metabolomics was implemented under supervised analysis according to fungal-bacteria interaction and time. Orthogonal partial least squares-discriminant analysis (OPLS-DA) model clearly discriminated P. indica–A. chroococcum and P. indica–S. anulatus interactions, according to the respective score plot in comparison to the control. The most observable responses were in the glutamine and alanine size groups: While Streptomyces AcH 1003 increased the amount of glutamine, A. chroococcum decreased it. The fungal growth and the increase of alanine content might be associated with the assimilation of nitrogen in the presence of glucose as a carbon source. The N-fixing bacterium A. chroococcum should stimulate fungal amino acid metabolism via glutamine synthetase-glutamate synthase (GS-GOGAT). The data pointed to a stimulated glycolytic activity in the fungus observed by the accumulation of alanine, possibly via alanine aminotransferase. The responses toward the growth-inhibiting Streptomyces AcH 1003 suggest an (oxidative) stress response of the fungus.pdfengMDPIAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2MoleculesVol. 25, No. 3 (2020)https://doi.org/10.3390/molecules25030572Piriformospora indicaEndophytic fungiMycorrhiza helper bacteriaAmino acid contentInteraction with soil bacteria affects the growth and amino acid content of piriformospora indicaarticlearticlehttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Deveau, A.; Bonito, G.; Uehling, J.; Paoletti, M.; Becker, M.; Bindschedler, S.; Hacquard, S.; Hervé, V.; Labbé, J.; Lastovetsky, O.A.; et al. Bacterial-fungal interactions: Ecology, mechanisms and challenges. FEMS Microbiol. Rev. 2018, 42, 335–352.Churchland, C.; Grayston, S.J. Specificity of plant-microbe interactions in the tree mycorrhizosphere biome and consequences for soil cycling. Front. Microbiol. 2014, 5, 1–20.Bonfante, P.; Anca, I.-A. Plants,mycorrhizal fungi, and bacteria: a network of interactions. Annu. Rev. Microbiol. 2009, 63, 363–383.Kim, M.; Yoon, H.; Kim, Y.E.; Kim, Y.J.; Kong, W.S.; Kim, J.G. Comparative analysis of bacterial diversity and communities inhabiting the fairy ring of Tricholoma matsutake by barcoded pyrosequencing. J. Appl. Microbiol. 2014, 117, 699–710.Schrey, S.D.; Tarkka, M.T. Friends and foes: Streptomycetes as modulators of plant disease and symbiosis. Antonie van Leeuwenhoek, Int. J. Gen. Mol. Microbiol. 2008, 94, 11–19.Xing, R.; Yan, H.Y.; Gao, Q.B.; Zhang, F.Q.; Wang, J.L.; Chen, S.L. Microbial communities inhabiting the fairy ring of Floccularia luteovirens and isolation of potential mycorrhiza helper bacteria. J. Basic Microbiol. 2018, 58, 554–563.Garbaye, J. Helper bacteria: A new dimension to the mycorrhizal symbiosis. New Phytol. 1994, 128, 197–210.Takács, T.; Cseresnyés, I.; Kovács, R.; Parádi, I.; Kelemen, B.; Szili-Kovács, T.; Füzy, A. Symbiotic e ectivity of dual and tripartite associations on soybean (Glycine max L. Merr.) cultivars inoculated with Bradyrhizobium japonicum and AM Fungi. Front. Plant Sci. 2018, 9, 1–14.Turrini, A.; Avio, L.; Giovannetti, M.; Agnolucci, M. Functional complementarity of arbuscular mycorrhizal fungi and associated microbiota: The challenge of translational research. Front. Plant Sci. 2018, 9, 10–13.Maier, A.; Riedlinger, J.; Fiedler, H.-P.; Hampp, R. Actinomycetales bacteria from a spruce stand: Characterization and effects on growth of root symbiotic and plant parasitic soil fungi in dual culture. Mycol. Prog. 2004, 3, 129–136.Riedlinger, J.; Schrey, S.D.; Tarkka, M.T.; Hampp, R.; Kapur, M.; Fiedler, H.-P. 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In Advances in Microbial Physiology; Academic Press: Cambridge, MA, USA, 2004; Volume 49, pp. 1–76. ISBN 0120277492.Ono, B.; Shirahige, Y.; Nanjoh, A.; Andou, N.; Ohue, H.; Ishino-Arao, Y. Cysteine biosynthesis in Saccharomyces cerevisiae: Mutation that confers cystathionine beta-synthase deficiency. J. Bacteriol. 1988, 170, 5883–5889.Savin, M.A.; Flavin, M. Cystationine synthesis in yeast: An alternative pathway for homocysteine biosynthesis. J. Bacteriol. 1972, 112, 299–303.Riedlinger, J. Die stoffichen Grundlagen der Modulation des Myzelwachstums von symbiotischen und pathogenen Pilzen durch Streptomyceten; Eberhard-Karls University of Tübingen: Tübingen, Germany, 2006.Ogita, A.; Matsumoto, K.; Fujita, K.; Usuki, Y.; Hatanaka, Y.; Tanaka, T. Synergistic fungicidal activities of amphotericin B andN-methyl-N”-dodecylguanidine: Aconstituent of polyol macrolide antibiotic niphimycin. J. Antibiot. (Tokyo) 2007, 60, 27–35.Käfer, E. 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Interaction with soil bacteria affects the growth and amino acid content of piriformospora indica

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