Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random library

Chagas disease, also known as American trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite Trypanosoma cruzi, which is transmitted by insects of the family Reduviidae. Since conventional treatments with nitroheterocyclic drugs show serious adverse reactions an...

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Autores:
Sáenz Garcia, José L.
Yamanaka, Isabel B.
Pacheco Lugo, Lisandro A.
Miranda, Juliana S.
Córneo, Emily S.
Machado de Ávila, Ricardo A.
De Moura, Juliana F.
DaRocha, Wanderson D.
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad Simón Bolívar
Repositorio:
Repositorio Digital USB
Idioma:
eng
OAI Identifier:
oai:bonga.unisimon.edu.co:20.500.12442/4528
Acceso en línea:
https://hdl.handle.net/20.500.12442/4528
Palabra clave:
Phage display
Trypanosoma cruzi
Surface
Epimastigotes
EPI18
Rights
License
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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network_name_str Repositorio Digital USB
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dc.title.eng.fl_str_mv Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random library
title Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random library
spellingShingle Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random library
Phage display
Trypanosoma cruzi
Surface
Epimastigotes
EPI18
title_short Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random library
title_full Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random library
title_fullStr Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random library
title_full_unstemmed Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random library
title_sort Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random library
dc.creator.fl_str_mv Sáenz Garcia, José L.
Yamanaka, Isabel B.
Pacheco Lugo, Lisandro A.
Miranda, Juliana S.
Córneo, Emily S.
Machado de Ávila, Ricardo A.
De Moura, Juliana F.
DaRocha, Wanderson D.
dc.contributor.author.none.fl_str_mv Sáenz Garcia, José L.
Yamanaka, Isabel B.
Pacheco Lugo, Lisandro A.
Miranda, Juliana S.
Córneo, Emily S.
Machado de Ávila, Ricardo A.
De Moura, Juliana F.
DaRocha, Wanderson D.
dc.subject.eng.fl_str_mv Phage display
Trypanosoma cruzi
Surface
Epimastigotes
EPI18
topic Phage display
Trypanosoma cruzi
Surface
Epimastigotes
EPI18
description Chagas disease, also known as American trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite Trypanosoma cruzi, which is transmitted by insects of the family Reduviidae. Since conventional treatments with nitroheterocyclic drugs show serious adverse reactions and have questionable efficiency, different research groups have investigated polypeptide-based approaches to interfere with the parasite cell cycle in other Trypanosomatids. These strategies are supported by the fact that surface players are candidates to develop surface ligands that impair function since they may act as virulence factors. In this study, we used a phage display approach to identify peptides from one library-LX8CX8 (17 aa) (where X corresponds to any amino acid). After testing different biopanning conditions using live or fixed epimastigotes, 10 clones were sequenced that encoded the same peptide, named here as EPI18. The bacteriophage expressing EPI18 binds to epimastigotes from distinct strains of T. cruzi. To confirm these results, this peptide was synthetized, biotinylated, and assayed using flow cytometry and confocal microscopy analyses. These assays confirmed the specificity of the binding capacity of EPI18 toward epimastigote surfaces. Our findings suggest that EPI18 may have potential biotechnological applications that include peptide-based strategies to control parasite transmission.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-01-16T19:35:00Z
dc.date.available.none.fl_str_mv 2020-01-16T19:35:00Z
dc.date.issued.none.fl_str_mv 2020-01
dc.type.eng.fl_str_mv article
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.identifier.issn.none.fl_str_mv 00144894
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12442/4528
identifier_str_mv 00144894
url https://hdl.handle.net/20.500.12442/4528
dc.language.iso.eng.fl_str_mv eng
language eng
dc.rights.eng.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.uri.eng.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.spa.fl_str_mv pdf
dc.publisher.spa.fl_str_mv Elsevier
dc.source.eng.fl_str_mv Experimental Parasitology
dc.source.none.fl_str_mv (2020)
institution Universidad Simón Bolívar
dc.source.uri.none.fl_str_mv https://doi.org/10.1016/j.exppara.2020.107830
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repository.name.fl_str_mv Repositorio Digital Universidad Simón Bolívar
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spelling Sáenz Garcia, José L.52e070f9-7c62-4873-bffa-f4a5971d8934Yamanaka, Isabel B.ed7d2053-ed46-4599-9841-54032a7001d5Pacheco Lugo, Lisandro A.b33a693b-0c82-4787-97aa-77ba97ed7da2Miranda, Juliana S.6039158e-5574-4d9e-9737-d2dc025ee2faCórneo, Emily S.fd1906ae-2d75-4ab6-878c-2d9496c00a0eMachado de Ávila, Ricardo A.5056728d-beb2-4492-aeab-1647273770d4De Moura, Juliana F.09933af4-b340-4e77-ac1e-505184fa638bDaRocha, Wanderson D.a9ad1c3a-be1c-40d9-b611-9474f6cb54b42020-01-16T19:35:00Z2020-01-16T19:35:00Z2020-0100144894https://hdl.handle.net/20.500.12442/4528Chagas disease, also known as American trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite Trypanosoma cruzi, which is transmitted by insects of the family Reduviidae. Since conventional treatments with nitroheterocyclic drugs show serious adverse reactions and have questionable efficiency, different research groups have investigated polypeptide-based approaches to interfere with the parasite cell cycle in other Trypanosomatids. These strategies are supported by the fact that surface players are candidates to develop surface ligands that impair function since they may act as virulence factors. In this study, we used a phage display approach to identify peptides from one library-LX8CX8 (17 aa) (where X corresponds to any amino acid). After testing different biopanning conditions using live or fixed epimastigotes, 10 clones were sequenced that encoded the same peptide, named here as EPI18. The bacteriophage expressing EPI18 binds to epimastigotes from distinct strains of T. cruzi. To confirm these results, this peptide was synthetized, biotinylated, and assayed using flow cytometry and confocal microscopy analyses. These assays confirmed the specificity of the binding capacity of EPI18 toward epimastigote surfaces. Our findings suggest that EPI18 may have potential biotechnological applications that include peptide-based strategies to control parasite transmission.pdfengElsevierAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_16ecExperimental Parasitology(2020)https://doi.org/10.1016/j.exppara.2020.107830Phage displayTrypanosoma cruziSurfaceEpimastigotesEPI18Targeting epimastigotes of trypanosoma cruzi with a peptide isolated from a phage display random libraryarticlehttp://purl.org/coar/resource_type/c_6501Adade et al., 2013 C.M. Adade, I.R.S. Oliveira, J.A.R. Pais, T. Souto-Padrón Melittin peptide kills Trypanosoma cruzi parasites by inducing different cell death pathways Toxicon, 69 (2013), pp. 227-239, 10.1016/j.toxicon.2013.03.011Altschul et al., 1990 S.F. Altschul, W. Gish, W. Miller, E.W. 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Biol., 250 (2017), pp. 115-122, 10.1007/s00232-016-9940-zCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805https://bonga.unisimon.edu.co/bitstreams/8c9d5d77-624b-4789-b3b8-df4d5950f748/download4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-8381https://bonga.unisimon.edu.co/bitstreams/1ff179fd-ea4e-48a2-8f9b-01acec270985/download733bec43a0bf5ade4d97db708e29b185MD5320.500.12442/4528oai:bonga.unisimon.edu.co:20.500.12442/45282024-08-14 21:52:41.124http://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internacionalmetadata.onlyhttps://bonga.unisimon.edu.coRepositorio Digital Universidad Simón Bolívarrepositorio.digital@unisimon.edu.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