Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia

Aedes aegypti is the main vector of dengue, chikungunya, and Zika viruses, which are of great public health importance in Colombia. Aedes control strategies in Colombia rely heavily on the use of organophosphate and pyrethroid insecticides, providing constant selection pressure and the emergence of...

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Autores:: Pareja-Loaiza, Paula X.
Santacoloma Varon, Liliana
Rey Vega, Gabriela
Gómez-Camargo, Doris
Maestre-Serrano, Ronald
Lenhart, Audrey

Tipo de recurso:

Fecha de publicación:: 2020

Institución:: Universidad Simón Bolívar

Repositorio:: Repositorio Digital USB

Idioma:: eng

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dc.title.eng.fl_str_mv	Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia
title	Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia
spellingShingle	Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia
title_short	Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia
title_full	Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia
title_fullStr	Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia
title_full_unstemmed	Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia
title_sort	Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia
dc.creator.fl_str_mv	Pareja-Loaiza, Paula X. Santacoloma Varon, Liliana Rey Vega, Gabriela Gómez-Camargo, Doris Maestre-Serrano, Ronald Lenhart, Audrey
dc.contributor.author.none.fl_str_mv	Pareja-Loaiza, Paula X. Santacoloma Varon, Liliana Rey Vega, Gabriela Gómez-Camargo, Doris Maestre-Serrano, Ronald Lenhart, Audrey
description	Aedes aegypti is the main vector of dengue, chikungunya, and Zika viruses, which are of great public health importance in Colombia. Aedes control strategies in Colombia rely heavily on the use of organophosphate and pyrethroid insecticides, providing constant selection pressure and the emergence of resistant populations. In recent years, insecticide use has increased due to the increased incidence of dengue and recent introductions of chikungunya and Zika. In the present study, pyrethroid resistance was studied across six populations of Ae. aegypti from the Caribbean coast of Colombia. Susceptibility to λ-cyhalothrin, deltamethrin, and permethrin was assessed, and resistance intensity was determined. Activity levels of enzymes associated with resistance were measured, and the frequencies of three kdr alleles (V1016I, F1534C, V410L) were calculated. Results showed variations in pyrethroid susceptibility across Ae. aegypti populations and altered enzyme activity levels were detected. The kdr alleles were detected in all populations, with high variations in frequencies: V1016I (frequency ranging from 0.15–0.70), F1534C (range 0.94–1.00), and V410L (range 0.05–0.72). In assays of phenotyped individuals, associations were observed between the presence of V1016I, F1534C, and V410L alleles and resistance to the evaluated pyrethroids, as well as between the VI1016/CC1534/VL410 tri-locus genotype and λ-cyhalothrin and permethrin resistance. The results of the present study contribute to the knowledge of the mechanisms underlying the resistance to key pyrethroids used to control Ae. aegypti along the Caribbean coast of Colombia.
publishDate	2020
dc.date.accessioned.none.fl_str_mv	2020-11-12T22:58:32Z
dc.date.available.none.fl_str_mv	2020-11-12T22:58:32Z
dc.date.issued.none.fl_str_mv	2020
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dc.type.spa.spa.fl_str_mv	Artículo científico
dc.identifier.issn.none.fl_str_mv	0228695
dc.identifier.uri.none.fl_str_mv	https://hdl.handle.net/20.500.12442/6794
dc.identifier.doi.none.fl_str_mv	https://doi.org/10.1371/journal.pone.0228695
dc.identifier.url.none.fl_str_mv	https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228695
identifier_str_mv	0228695
url	https://hdl.handle.net/20.500.12442/6794 https://doi.org/10.1371/journal.pone.0228695 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228695
dc.language.iso.eng.fl_str_mv	eng
language	eng
dc.rights.*.fl_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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dc.format.mimetype.spa.fl_str_mv	pdf
dc.publisher.eng.fl_str_mv	Plos
dc.source.eng.fl_str_mv	Plos One
dc.source.none.fl_str_mv	Vol. 15, No 10 (2020)
institution	Universidad Simón Bolívar
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spelling	Pareja-Loaiza, Paula X.fa464c12-d1de-47ad-866d-529b391645d0Santacoloma Varon, Liliana8638a0a5-0098-4a94-8373-7841d5ead721Rey Vega, Gabrielaa1d021f0-4f5b-427d-859f-d19310ee567fGómez-Camargo, Dorise317a713-0a9e-4f39-b62a-7aecc46df56eMaestre-Serrano, Ronald40ba2456-d23d-4a6d-8d98-e4dbf3fefc19Lenhart, Audrey93f946ee-e877-4217-9494-1a9411c9746e2020-11-12T22:58:32Z2020-11-12T22:58:32Z20200228695https://hdl.handle.net/20.500.12442/6794https://doi.org/10.1371/journal.pone.0228695https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228695Aedes aegypti is the main vector of dengue, chikungunya, and Zika viruses, which are of great public health importance in Colombia. Aedes control strategies in Colombia rely heavily on the use of organophosphate and pyrethroid insecticides, providing constant selection pressure and the emergence of resistant populations. In recent years, insecticide use has increased due to the increased incidence of dengue and recent introductions of chikungunya and Zika. In the present study, pyrethroid resistance was studied across six populations of Ae. aegypti from the Caribbean coast of Colombia. Susceptibility to λ-cyhalothrin, deltamethrin, and permethrin was assessed, and resistance intensity was determined. Activity levels of enzymes associated with resistance were measured, and the frequencies of three kdr alleles (V1016I, F1534C, V410L) were calculated. Results showed variations in pyrethroid susceptibility across Ae. aegypti populations and altered enzyme activity levels were detected. The kdr alleles were detected in all populations, with high variations in frequencies: V1016I (frequency ranging from 0.15–0.70), F1534C (range 0.94–1.00), and V410L (range 0.05–0.72). In assays of phenotyped individuals, associations were observed between the presence of V1016I, F1534C, and V410L alleles and resistance to the evaluated pyrethroids, as well as between the VI1016/CC1534/VL410 tri-locus genotype and λ-cyhalothrin and permethrin resistance. The results of the present study contribute to the knowledge of the mechanisms underlying the resistance to key pyrethroids used to control Ae. aegypti along the Caribbean coast of Colombia.pdfengPlosAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Plos OneVol. 15, No 10 (2020)Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombiainfo:eu-repo/semantics/articleArtículo científicohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_2df8fbb1Padilla JC, Lizarazo FE, Murillo OL, Mendigaña FA, Pacho´n E, Vera MJ. Epidemiologı´a de las principales enfermedades transmitidas por vectores en Colombia, 1990–2016. Biomedica. 2017; 37 (Supl.2):27–40.World Health Organization. Dengue y dengue grave [Internet]. [cited 2018 Apr 30]. Available from: https://www.who.int/es/news-room/fact-sheets/detail/dengue-and-severe-dengueInstituto Nacional de Salud. Informe de evento. Dengue: Colombia, 2017 [Internet]. [cited 2018 May 20]. Available from: https://www.ins.gov.co/buscador-eventos/Informesdeevento/DENGUE2017.pdfInstituto Nacional de Salud. Sistema de Vigilancia Epidemiologico Nacional: Enfermedades Transmitidas por Vectores [Internet]. [cited 2018 May 23]. Available from: http://portalsivigila.ins.gov.co/sivigila/ documentos/Docs_1.phpTovar-Sanchez Z, Bolívar_pertuz S, Maestre-Serrano R. Chikungunya: aspectos generales de una enfermedad emergente en Colombia Chikungunya: general aspects of an emerging disease in Colombia. Rev Biociencias. 2015; 10(1):75–88.Instituto Nacional de Salud. Informe de evento: Enfermedad por virus Zika. Colombia, 2017 [Internet]. [cited 2018 May 23]. Available from: http://www.ins.gov.co/buscador-eventos/Informesdeevento/ ZIKA2017.pdfMaestre-Serrano R, Pacheco-Lugo L, Salcedo-Mendoza S.I´ndices de infestación aédica e identificación de conocimientos, actitudes y prácticas sobre dengue en llanterías del Departamento del Atlántico, Colombia. (Spanish). Rev Salud Pu´ blica [Internet]. 2015; 17(5):738–48. Available from: http://10.0.60. 86/rsap.v17n5.3534%5Cnhttp://search.ebscohost.com/login.aspx?direct=true&db=lth&AN= 113247085&lang=es&site=ehost-live https://doi.org/10.15446/rsap.v17n5.35345 PMID: 28453051Ardila-Roldán S, Santacoloma L, Brochero H. Estado de la sensibilidad a los insecticidas de uso en salud pública en poblaciones naturales de Aedes aegypti (Diptera: Culicidae) del departamento de Casanare, Colombia. Biomedica. 2013; 33(3):446–58. https://doi.org/10.7705/biomedica.v33i3.1534 PMID: 24652181Conde M, Orjuela LI, Castellanos CA, Herrera-Varela M, Licastro S, Quiñones ML. Evaluación de la sensibilidad a insecticidas en poblaciones de Aedes aegypti (Diptera: Culicidae) del departamento de Caldas, Colombia, en 2007 y 2011. Biomedica. 2015; 35(1):43–52. https://doi.org/10.1590/S0120- 41572015000100007 PMID: 26148033Bisset LJ a. Uso correcto de insecticidas: control de la resistencia. Rev Cubana Med Trop. 2002; 54 (3):202–19. PMID: 15846946Fonseca-González I BD. Variación temporal en la susceptibilidad a malatión y lambdacialotrina en Aedes aegypti (L) de Quibdó , Colombia. In: Biomedica, editor. Congreso de Parasitologia y Medicina Tropical. Ibague; 2009. p. 216–34.Maestre-Serrano R, Rey Gabriela, De las salas J, Vergara C, Santacoloma L, Goenaga S, et al. Estado de la susceptibilidad de Aedes aegypti a insecticidas en Atlántico (Colombia). Rev Colomb Entomol. 2010; 36(2):242–8.Santacoloma L, Chaves B, Brochero HL. Estado de la susceptibilidad de poblaciones naturales del vector del dengue a insecticidas en trece localidades de Colombia. Biomedica. 2012; 32(3):333–43. https:// doi.org/10.1590/S0120-41572012000300004 PMID: 23715182Instituto Nacional de Salud. Red de vigilancia de la resistencia a insecticidas de uso en salud pública en Colombia, año 2018 [Internet]. [cited 2018 May 20]. Available from: http://www.ins.gov.co/buscadoreventos/ Informacindelaboratorio/Informe-VRI-2018.pdfRodríguez M, BIsset M, Ditter F, Omayda P. Resistencia a insecticidas en larvas y adultos de Aedes aegypti: prevalencia de la esterasa A4 asociada con la resistencia a temefos. Rev Cubana Med Trop. 2004; 56(1):54–60. PMID: 15849910Rodríguez MM, Bisset JA, Ricardo Y, Pérez O, Montada D, Figueredo D, et al. Resistencia a insecticidas organofosforados en Aedes aegypti (Diptera: Culicidae) de Santiago de Cuba, 1997–2009. Rev Cubana Med Trop [Internet]. 2010; 62(3):217–23. Available from: http://scielo.sld.cu/scielo.php?script= sci_arttext&pid=S0375-07602010000300009&lng=es&nrm=iso&tlng=es PMID: 23437552Maestre-Serrano R. Susceptibility status of Aedes aegypti to insecticides in Colombia. In: Insecticides– Pest Engineering [Internet]. 2012. p. 163–200. Available from: http://www.scielo.org.co/scielo.php? script=sci_arttext&pid=S0120-04882010000200012&lng=en&nrm=iso&tlng=esSantacoloma L, Chaves B, Brochero H. Susceptibilidad de Aedes aegypti a DDT, deltametrina y lambdacialotrina en Colombia. Rev Panam Salud Publica/Pan Am J Public Heal. 2010; 27(1):66–73.Fonseca-González I, Quiñones ML, Lenhart A, Brogdon WG. Insecticide resistance status of Aedes aegypti (L.) from Colombia. Pest Manag Sci. 2011; 67(4):430–7. https://doi.org/10.1002/ps.2081 PMID: 21394876Ocampo CB, Salazar-Terreros MJ, Mina NJ, McAllister J, Brogdon W. Insecticide resistance status of Aedes aegypti in 10 localities in Colombia. Acta Trop [Internet]. 2011; 118(1):37–44. Available from: https://doi.org/10.1016/j.actatropica.2011.01.007 PMID: 21300017Maestre-Serrano R, Gomez-Camargo D, Ponce-Garcia G, Flores AE. Susceptibility to insecticides and resistance mechanisms in Aedes aegypti from the Colombian Caribbean Region. Pestic Biochem Physiol [Internet]. 2014; 116:63–73. Available from: https://doi.org/10.1016/j.pestbp.2014.09.014 PMID: 25454522Granada Y, Mar A, Strode C, Triana-chavez O. A Point Mutation V419L in the Sodium Channel Gene from Natural Populations of Aedes aegypti Is Involved in Resistance to λ -Cyhalothrin in Colombia. Insects. 2018; 9:23–35.Du Y, Nomura Y, Zhorov BS, Dong K. Sodium channel mutations and pyrethroid resistance in Aedes aegypti. Insects. 2016; 7(4):60–71.Salazar M, Carvajal A, Cuellar ME, Olaya A, Quiñones J, Velasquez OL, et al. Resistance to insecticides in populations of Aedes aegypti and Anopheles spp. in the departments of Huila, Valle Cauca and Nariño. In: Biomedica, editor. XIII Congreso colombiano de parasitologia y medicina tropical. Bogota; 2007. p. 177.Maestre RS, Rey G V., De Las J, Vergara CS, Santacoloma L V., Goenaga SO, et al. Susceptibilidad de Aedes aegypti (Diptera: Culicidae) a temefos en Atlantico-Colombia. Rev Colomb Entomol. 2009; 35 (2):202–5.Maestre-Serrano R, Florez Z, Cabrera C, Goenaga S, Gomez D GC. Susceptibility status of Aedes aegypti to insecticides in la Guajira (colombia). In: Hygiene TAJ of TM and, editor. The American Journal of Tropical Medicine and Hygiene. Atlanta, Georgia; 2010. p. 99–141.Grisales N, Poupardin R, Gomez S, Fonseca-Gonzalez I, Ranson H, Lenhart A. Temephos Resistance in Aedes aegypti in Colombia Compromises Dengue Vector Control. PLoS Negl Trop Dis. 2013; 7(9).Aguirre-Obando OA, Dalla Bona AC, Duque L JE, Navarro-Silva MA. Insecticide resistance and genetic variability in natural populations of Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Colombia. Zoologia. 2015; 32(1):14–22.Suárez MF, González R MC. Temefos resistance to Aedes aegypti in Cali, Colombia. In: Am J Trop Med Hyg, editor. 45th Annual meeting of the American Society of Tropical Medicine and Hygiene. Baltimore, Maryland; 1996. p. 257.Rojas W, González J, Amud M, Quiñones M VI. Evaluation of the susceptibility of Aedes aegypti of the municipality of Barrancabermeja, Santander, to the insecticides malathion, fenitrothion, temephos, lambda-cyhalothrin, deltamethrin, permethrin, pro. In: Biomedica, editor. Congreso colombiano de medicina tropical. 2003. p. 56.Anaya Y, Cochero S, Rey G S l. Assessment of susceptibility to insecticides of Aedes aegypti caught in Sincelejo. In: Biomedica, editor. XIII Congreso colombiano de parasitologia y medicina tropical. Bogota; 2007. p. 257.Aguirre-Obando OA, Martins AJ, Navarro-Silva MA. First report of the Phe1534Cys kdr mutation in natural populations of Aedes albopictus from Brazil. Parasites and Vectors. 2017; 10(1):160–70. https:// doi.org/10.1186/s13071-017-2089-5 PMID: 28347326Atencia MC, Pérez M de J, Jaramillo MC, Caldera SM, Cochero S, Bejarano EE. First report of the F1534C mutation associated with cross-resistance to DDT and pyrethroids in Aedes aegypti from Colombia. Biomedica. 2016; 36(3):432–7. https://doi.org/10.7705/biomedica.v36i3.2834 PMID: 27869391Maestre-Serrano R, Pareja-Loaiza P, Gomez Camargo D, Ponce-García G, Flores AE. Co-occurrence of V1016I and F1534C mutations in the voltage-gated sodium channel and resistance to pyrethroids in Aedes aegypti (L.) from the Colombian Caribbean region. Pest Manag Sci. 2019; 75(6):1681–8. https:// doi.org/10.1002/ps.5287 PMID: 30520256Brogdon WG, McAllister JC. Insecticide resistance and vector control. Emerg Infect Dis. 1998; 9 (2):605–13.World Health Organization—WHO. Test procedures for insecticide resistance monitoring in malaria vector mosquitoes. Second. Ginebra; 2017. 1–50 p.Valle D, Montella IR. Quantification methodology for enzyme activity related to insecticide resistance in Aedes aegypti. Vol. 1, Brasil. Ministe´ rio da Sau´de. Fundac¸ão Oswaldo Cruz. 2006. 128 p.Brogdon WG. Mosquito protein microassay-I. Protein determinations from small portions of single-mosquito homogenates. Comp Biochem Physiol—Part B Biochem. 1984; 79B(3):457–9.Saavedra-Rodriguez K, Urdaneta-Marquez L, Rajatileka S, Moulton M, Flores AE, Fernandez-Salas I, et al. A mutation in the voltage-gated sodium channel gene associated with pyrethroid resistance in Latin American Aedes aegypti. Insect Mol Biol. 2007; 16(6):785–98. https://doi.org/10.1111/j.1365- 2583.2007.00774.x PMID: 18093007Yanola J, Somboon P, Walton C, Nachaiwieng W, Somwang P, Prapanthadara L aied. Analyses à haut dé bit pour la dé tection de la mutation F1534C dans le gène du canal sodium voltage dépendant d’Aedes aegypti résistant au permé thrine et distribution de cette mutation à travers la Thaïlande. Trop Med Int Heal. 2011; 16(4):501–9.Haddi K, Tomé HVV, Du Y, Valbon WR, Nomura Y, Martins GF, et al. Detection of a new pyrethroid resistance mutation (V410L) in the sodium channel of Aedes aegypti: A potential challenge for mosquito control. Sci Rep [Internet]. 2017; 7(March):1–9. Available from: http://dx.doi.org/10.1038/srep46549Montella IR, Martins AJ, Viana-Medeiros PF, Lima JBP, Braga IA, Valle D. Insecticide resistance mechanisms of Brazilian Aedes aegypti populations from 2001 to 2004. Am J Trop Med Hyg. 2007; 77 (3):467–77. PMID: 17827362Zlotkin E, Devonshire AL, Warmke JW. The pharmacological flexibility of the insect voltage gated sodium channel: Toxicity of AaIT to knockdown resistant (kdr) flies. Insect Biochem Mol Biol. 1999; 29 (10):849–53. https://doi.org/10.1016/s0965-1748(99)00079-x PMID: 10528405Rodríguez MM, Bisset J, Ruiz M, Soca A. Cross-Resistance to Pyrethroid and Organophosphorus Insecticides Induced by Selection with Temephos in Aedes aegypti (Diptera: Culicidae) from Cuba. J Med Entomol. 2002; 39(6):882–8. https://doi.org/10.1603/0022-2585-39.6.882 PMID: 12495187Tikar SN, Kumar A, Prasad GBKS, Prakash S. Temephos-induced resistance in Aedes aegypti and its cross-resistance studies to certain insecticides from India. Parasitol Res. 2009; 105(1):57–63. https:// doi.org/10.1007/s00436-009-1362-8 PMID: 19229558Aïzoun N, Ossè R, Azondekon R, Alia R, Oussou O, Gnanguenon V, et al. Comparison of the standard WHO susceptibility tests and the CDC bottle bioassay for the determination of insecticide susceptibility in malaria vectors and their correlation with biochemical and molecular biology assays in Benin, West Africa. Parasites and Vectors. 2013; 6(1):147–56.Flores AE, Albeldaño-Vázquez W, Salas IF, Badii MH, Becerra HL, Garcia GP, et al. Elevated α-esterase levels associated with permethrin tolerance in Aedes aegypti (L.) from Baja California, Mexico. Pestic Biochem Physiol. 2005; 82(1):66–78.Flores AE, Grajales JS, Salas IF, Garcia GP, Becerra HL, Lozano S, et al. Mechanisms of Insecticide Resistance in Field Populations of Aedes aegypti (L.) From Mechanisms of Insecticide Resistance in Field Populations. 2006; 22(4):672–7. https://doi.org/10.2987/8756-971X(2006)22[672:MOIRIF]2.0. CO;2 PMID: 17304936Harris AF, Rajatileka S, Ranson H. Pyrethroid resistance in Aedes aegypti from Grand Cayman. Am J Trop Med Hyg. 2010; 83(2):277–84. https://doi.org/10.4269/ajtmh.2010.09-0623 PMID: 20682868Polson KA, Brogdon WG, Rawlins SC, Chadee DD. Characterization of insecticide resistance in Trinidadian strains of Aedes aegypti mosquitoes. Acta Trop [Internet]. 2011; 117(1):31–8. Available from: https://doi.org/10.1016/j.actatropica.2010.09.005 PMID: 20858454Alvarez LC, Ponce G, Oviedo M, Lopez B, Flores AE. Resistance to Malathion and Deltamethrin in Aedes aegypti (Diptera: Culicidae) From Western Venezuela. J Med Entomol. 2013; 50(5):1031–9. https://doi.org/10.1603/me12254 PMID: 24180108Saavedra-Rodriguez K, Maloof FV, Campbell CL, Garcia-Rejon J, Lenhart A, Penilla P, et al. Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico. Sci Rep. 2018; 8(1):6749–55. https://doi.org/10.1038/s41598-018-24642-2 PMID: 29712940Liu N. Insecticide Resistance in Mosquitoes: Impact, Mechanisms, and Research Directions. Annu Rev Entomol. 2015; 60(1):537–59.Angélica Aponte, R. Patricia Penilla, Rodríguez Américo D.and CBO. Mechanisms of Pyrethroid Resistance in Aedes (Stegomyia) aegypti from Colombia. 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Mechanisms associated with pyrethroid resistance in populations of Aedes aegypti (Diptera: Culicidae) from the Caribbean coast of Colombia

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