Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats

Methylmercury (MeHg) is an organic bioaccumulated mercury derivative that strongly affects the environment and represents a public health problem primarily to riparian communities in South America. Our objective was to investigate the hepatic and neurological effects of MeHg exposure during the phas...

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Autores:
da Rosa-Silva, Helen Tais
Castro Panzenhagen, Alana
Schmidtt, Victória
Alves Teixeira, Alexsander
Espitia-Pérez, Pedro
de Oliveira Franco, Álvaro
Mingori, Moara
Torres-Ávila, José F.
Schnorr, Carlos Eduardo
Silva Hermann, Paolla Rissi
Pompéu Moraes, Diogo
Farina Almeida, Roberto
Fonseca Moreira, José Cláudio
Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad Simón Bolívar
Repositorio:
Repositorio Digital USB
Idioma:
eng
OAI Identifier:
oai:bonga.unisimon.edu.co:20.500.12442/4410
Acceso en línea:
https://hdl.handle.net/20.500.12442/4410
Palabra clave:
Methylmercury
Double exposure
Double exposure
Hepatotoxicity
Neurotoxicity
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License
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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oai_identifier_str oai:bonga.unisimon.edu.co:20.500.12442/4410
network_acronym_str USIMONBOL2
network_name_str Repositorio Digital USB
repository_id_str
dc.title.eng.fl_str_mv Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats
title Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats
spellingShingle Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats
Methylmercury
Double exposure
Double exposure
Hepatotoxicity
Neurotoxicity
title_short Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats
title_full Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats
title_fullStr Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats
title_full_unstemmed Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats
title_sort Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats
dc.creator.fl_str_mv da Rosa-Silva, Helen Tais
Castro Panzenhagen, Alana
Schmidtt, Victória
Alves Teixeira, Alexsander
Espitia-Pérez, Pedro
de Oliveira Franco, Álvaro
Mingori, Moara
Torres-Ávila, José F.
Schnorr, Carlos Eduardo
Silva Hermann, Paolla Rissi
Pompéu Moraes, Diogo
Farina Almeida, Roberto
Fonseca Moreira, José Cláudio
dc.contributor.author.none.fl_str_mv da Rosa-Silva, Helen Tais
Castro Panzenhagen, Alana
Schmidtt, Victória
Alves Teixeira, Alexsander
Espitia-Pérez, Pedro
de Oliveira Franco, Álvaro
Mingori, Moara
Torres-Ávila, José F.
Schnorr, Carlos Eduardo
Silva Hermann, Paolla Rissi
Pompéu Moraes, Diogo
Farina Almeida, Roberto
Fonseca Moreira, José Cláudio
dc.subject.eng.fl_str_mv Methylmercury
Double exposure
Double exposure
Hepatotoxicity
Neurotoxicity
topic Methylmercury
Double exposure
Double exposure
Hepatotoxicity
Neurotoxicity
description Methylmercury (MeHg) is an organic bioaccumulated mercury derivative that strongly affects the environment and represents a public health problem primarily to riparian communities in South America. Our objective was to investigate the hepatic and neurological effects of MeHg exposure during the phases foetal and breast-feeding and adult in Wistar rats. Wistar rats (n = 10) were divided into 3 groups. Control group received mineral oil; The simple exposure (SE) group was exposed only in adulthood (0.5 mg/kg/day); and double exposure (DE) was pre-exposed to MeHg 0.5 mg/kg/day during pregnancy and breastfeeding (±40 days) and re-exposed to MeHg for 45 days from day 100. After, we evaluated possible abnormalities. Behavioral and biochemical parameters in liver and occipital cortex (CO), markers of liver injury, redox and AKT/GSK3b/mTOR signaling pathway. Our results showed that both groups treated with MeHg presented significant alterations, such as decreased locomotion and exploration and impaired visuospatial perception. The rats exposed to MeHg showed severe liver damage and increased hepatic glycogen concentration. The MeHg groups showed significant impairment in redox balance and oxidative damage to liver macromolecules and CO. MeHg upregulated the AKT/GSK3b/mTOR pathway and the phosphorylated form of the Tau protein. In addition, we found a reduction in NeuN and GFAP immunocontent. These results represent the first approach to the hepatotoxic and neural effects of foetal and adult MeHg exposure.
publishDate 2019
dc.date.accessioned.none.fl_str_mv 2019-12-06T14:38:26Z
dc.date.available.none.fl_str_mv 2019-12-06T14:38:26Z
dc.date.issued.none.fl_str_mv 2019
dc.type.eng.fl_str_mv article
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.identifier.issn.none.fl_str_mv 00456535
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12442/4410
identifier_str_mv 00456535
url https://hdl.handle.net/20.500.12442/4410
dc.language.iso.eng.fl_str_mv eng
language eng
dc.rights.*.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_f1cf
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
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dc.publisher.eng.fl_str_mv ELSevier
dc.source.eng.fl_str_mv Chemosphere
institution Universidad Simón Bolívar
dc.source.uri.eng.fl_str_mv https://doi.org/10.1016/j.chemosphere.2019.125400
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spelling da Rosa-Silva, Helen Tais4c442548-a0b2-43d9-ac03-96017b3d60acCastro Panzenhagen, Alana68e9c798-0c2e-46a2-8f91-3323bc7a72fbSchmidtt, Victória689d7dbf-45c0-4c09-9701-ecb751c9eed4Alves Teixeira, Alexsanderff66c307-0183-4c90-9cc7-15f7effe69d1Espitia-Pérez, Pedro9b91d650-d997-4d18-8816-7533217bbe79de Oliveira Franco, Álvaro412c8981-2dae-4c4d-9cf9-171391e2653bMingori, Moara51974db1-9473-4a4d-842b-7cc075440d63Torres-Ávila, José F.0ecb74af-8314-44ae-aa75-11fbc8748507Schnorr, Carlos Eduardofe2833bc-5c20-47a1-9604-3a95a52fef41Silva Hermann, Paolla Rissi32fd7f06-88e1-4038-ab50-5dfdd509ff3cPompéu Moraes, Diogo26103bc2-11f3-43eb-ada9-db22aa479b2aFarina Almeida, Robertodde9fffa-32db-4fd6-8734-282d8c2dc6d3Fonseca Moreira, José Cláudio577cca00-aad4-4c12-b6ed-1c5c1da452312019-12-06T14:38:26Z2019-12-06T14:38:26Z201900456535https://hdl.handle.net/20.500.12442/4410Methylmercury (MeHg) is an organic bioaccumulated mercury derivative that strongly affects the environment and represents a public health problem primarily to riparian communities in South America. Our objective was to investigate the hepatic and neurological effects of MeHg exposure during the phases foetal and breast-feeding and adult in Wistar rats. Wistar rats (n = 10) were divided into 3 groups. Control group received mineral oil; The simple exposure (SE) group was exposed only in adulthood (0.5 mg/kg/day); and double exposure (DE) was pre-exposed to MeHg 0.5 mg/kg/day during pregnancy and breastfeeding (±40 days) and re-exposed to MeHg for 45 days from day 100. After, we evaluated possible abnormalities. Behavioral and biochemical parameters in liver and occipital cortex (CO), markers of liver injury, redox and AKT/GSK3b/mTOR signaling pathway. Our results showed that both groups treated with MeHg presented significant alterations, such as decreased locomotion and exploration and impaired visuospatial perception. The rats exposed to MeHg showed severe liver damage and increased hepatic glycogen concentration. The MeHg groups showed significant impairment in redox balance and oxidative damage to liver macromolecules and CO. MeHg upregulated the AKT/GSK3b/mTOR pathway and the phosphorylated form of the Tau protein. In addition, we found a reduction in NeuN and GFAP immunocontent. These results represent the first approach to the hepatotoxic and neural effects of foetal and adult MeHg exposure.engELSevierAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_f1cfChemospherehttps://doi.org/10.1016/j.chemosphere.2019.125400MethylmercuryDouble exposureDouble exposureHepatotoxicityNeurotoxicityHepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar ratsarticlehttp://purl.org/coar/resource_type/c_6501Aebi, H., 1984. [13] Catalase in vitro. Oxygen Radicals in Biological Systems, vol. 105. Academic Press, pp. 121e126. https://doi.org/10.1016/S0076-6879(84)05016-3.Almeida, R.F., et al., 2010. Systemic administration of GMP induces anxiolytic-like behavior in rats. Pharmacol. Biochem. Behav. 96 (3), 306e311. https://doi.org/ 10.1016/j.pbb.2010.05.022.Amorim, M.I., Mergler, D., Bahia, M.O., Dubeau, H., Miranda, D., Lebel, J., et al., 2000. Cytogenetic damage related to low levels of methyl mercury contamination in the Brazilian Amazon. An Acad. Bras Ci^encias 72, 497e507.Baraldi, M., Caselgrandi, E., Borella, P., Zeneroli, M.L., 1983. Decrease of brain zinc in experimental hepatic encephalopathy. Brain Res. https://doi.org/10.1016/0006- 8993(83)91246-5.Bellinger, D.C., et al., 2016. Country-specific estimates of the incidence of intellectual disability associated with prenatal exposure to methylmercury. Environ. 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