Reconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthi

La producción de clavel a nivel mundial sufre grandes pérdidas por el marchitamiento vascular causado por el patógeno Fod (Fusarium oxysporum f. sp. dianthi). Por lo que Los trabajos relacionados con el mecanismo de respuesta de variedades naturalmente resistentes, podría ser el primer paso para la...

Full description

Autores:
Morales Villanueva, Carlos Alberto
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2022
Institución:
Universidad Militar Nueva Granada
Repositorio:
Repositorio UMNG
Idioma:
spa
OAI Identifier:
oai:repository.unimilitar.edu.co:10654/44529
Acceso en línea:
http://hdl.handle.net/10654/44529
Palabra clave:
miRNA
Carnation
Fusarium oxysporum
Dianthus caryophyllus
FUSARIUM OXYSPORUM
DIANTHUS CARYOPHYLLUS
ORGANISMOS PATOGENOS
miARN
Clavel
Fusarium oxysporum
Dianthus caryophyllus
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-nd/4.0/
id UNIMILTAR2_8e16f1b985e8c35e119fa69095958c13
oai_identifier_str oai:repository.unimilitar.edu.co:10654/44529
network_acronym_str UNIMILTAR2
network_name_str Repositorio UMNG
repository_id_str
dc.title.spa.fl_str_mv Reconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthi
dc.title.translated.spa.fl_str_mv Recognition of miRNAs and their relationship with the expression of carnation (Dianthus caryophyllus) genes involved in the response to the pathogen Fusarium oxysporum f. sp. dianthi
title Reconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthi
spellingShingle Reconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthi
miRNA
Carnation
Fusarium oxysporum
Dianthus caryophyllus
FUSARIUM OXYSPORUM
DIANTHUS CARYOPHYLLUS
ORGANISMOS PATOGENOS
miARN
Clavel
Fusarium oxysporum
Dianthus caryophyllus
title_short Reconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthi
title_full Reconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthi
title_fullStr Reconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthi
title_full_unstemmed Reconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthi
title_sort Reconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthi
dc.creator.fl_str_mv Morales Villanueva, Carlos Alberto
dc.contributor.author.none.fl_str_mv Morales Villanueva, Carlos Alberto
dc.subject.keywords.spa.fl_str_mv miRNA
Carnation
Fusarium oxysporum
Dianthus caryophyllus
topic miRNA
Carnation
Fusarium oxysporum
Dianthus caryophyllus
FUSARIUM OXYSPORUM
DIANTHUS CARYOPHYLLUS
ORGANISMOS PATOGENOS
miARN
Clavel
Fusarium oxysporum
Dianthus caryophyllus
dc.subject.agrovoc.spa.fl_str_mv FUSARIUM OXYSPORUM
DIANTHUS CARYOPHYLLUS
ORGANISMOS PATOGENOS
dc.subject.proposal.spa.fl_str_mv miARN
Clavel
Fusarium oxysporum
Dianthus caryophyllus
description La producción de clavel a nivel mundial sufre grandes pérdidas por el marchitamiento vascular causado por el patógeno Fod (Fusarium oxysporum f. sp. dianthi). Por lo que Los trabajos relacionados con el mecanismo de respuesta de variedades naturalmente resistentes, podría ser el primer paso para la producción de variedades comerciales resistentes a Fod. En este estudio se fabricaron librerías de miRNA de dos variedades. 395 resistente y SH2 susceptible a Fod, en dos tratamientos: antes de la elicitación y después de la elicitación. Se encontraron miRNAs putativos que regulan la expresión de alguno de los 35 genes muestra. Estos genes muestran variaciones importantes en sus niveles de expresión durante la elicitación con Fod. Esto se determinó en trabajos anteriores en el laboratorio de Biotecnología vegetal de la Universidad Militar Nueva Granada. Estos miRNAs fueron validados y su perfil de control postranscripcional fue relacionado con el mecanismo de respuesta de ambas variedades. Se encontró que ambas variedades responden mediante el mismo perfil de control postranscripcional y tienen un mecanismo de respuesta similar. Sin embargo, 395 controla postranscripcionalmente los genes adecuados en el momento oportuno para responder de manera efectiva. Mientras que SH2 no tiene esa misma eficiencia, lo que no le permite ser oportuno en la respuesta ante Fod. Se proponen 84 miRNAs putativos en total que están controlando los mecanismos de expresión de 26 de los 35 genes al momento de la elicitación. Realizar experimentos de “Knock out” o “Knock down” sobre estos 84 miRNAs podría mostrar perdida o ganancia de resistencia a Fod, lo que permitiría concluir que estos miRNAs son claves para el mecanismo de respuesta de variedades resistentes de clavel ante Fod.
publishDate 2022
dc.date.issued.none.fl_str_mv 2022-11-21
dc.date.accessioned.none.fl_str_mv 2023-06-13T20:01:03Z
dc.date.available.none.fl_str_mv 2023-06-13T20:01:03Z
dc.type.local.spa.fl_str_mv Tesis/Trabajo de grado - Monografía - Pregrado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/bachelorThesis
dc.type.coar.*.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
format http://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10654/44529
dc.identifier.instname.spa.fl_str_mv instname:Universidad Militar Nueva Granada
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional Universidad Militar Nueva Granada
dc.identifier.repourl.spa.fl_str_mv repourl:https://repository.unimilitar.edu.co
url http://hdl.handle.net/10654/44529
identifier_str_mv instname:Universidad Militar Nueva Granada
reponame:Repositorio Institucional Universidad Militar Nueva Granada
repourl:https://repository.unimilitar.edu.co
dc.language.iso.spa.fl_str_mv spa
language spa
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spelling Morales Villanueva, Carlos AlbertoBiólogo2023-06-13T20:01:03Z2023-06-13T20:01:03Z2022-11-21http://hdl.handle.net/10654/44529instname:Universidad Militar Nueva Granadareponame:Repositorio Institucional Universidad Militar Nueva Granadarepourl:https://repository.unimilitar.edu.coLa producción de clavel a nivel mundial sufre grandes pérdidas por el marchitamiento vascular causado por el patógeno Fod (Fusarium oxysporum f. sp. dianthi). Por lo que Los trabajos relacionados con el mecanismo de respuesta de variedades naturalmente resistentes, podría ser el primer paso para la producción de variedades comerciales resistentes a Fod. En este estudio se fabricaron librerías de miRNA de dos variedades. 395 resistente y SH2 susceptible a Fod, en dos tratamientos: antes de la elicitación y después de la elicitación. Se encontraron miRNAs putativos que regulan la expresión de alguno de los 35 genes muestra. Estos genes muestran variaciones importantes en sus niveles de expresión durante la elicitación con Fod. Esto se determinó en trabajos anteriores en el laboratorio de Biotecnología vegetal de la Universidad Militar Nueva Granada. Estos miRNAs fueron validados y su perfil de control postranscripcional fue relacionado con el mecanismo de respuesta de ambas variedades. Se encontró que ambas variedades responden mediante el mismo perfil de control postranscripcional y tienen un mecanismo de respuesta similar. Sin embargo, 395 controla postranscripcionalmente los genes adecuados en el momento oportuno para responder de manera efectiva. Mientras que SH2 no tiene esa misma eficiencia, lo que no le permite ser oportuno en la respuesta ante Fod. Se proponen 84 miRNAs putativos en total que están controlando los mecanismos de expresión de 26 de los 35 genes al momento de la elicitación. Realizar experimentos de “Knock out” o “Knock down” sobre estos 84 miRNAs podría mostrar perdida o ganancia de resistencia a Fod, lo que permitiría concluir que estos miRNAs son claves para el mecanismo de respuesta de variedades resistentes de clavel ante Fod.Carnation production worldwide suffers great losses due to vascular wilt caused by the pathogen Fod (Fusarium oxysporum f. sp. dianthi). Therefore, the work related to the response mechanism of naturally resistant varieties could be the first step to produce commercial varieties naturally resistant to Fod. In this study, miRNA libraries of two varieties were fabricated. 395 resistant and SH2 susceptible, in two treatments: before elicitation and after elicitation. Putative miRNAs that regulate the expression of some of the 35 sample genes were found. These genes show important variations in their expression levels upon chemical contact with Fod. This was determined in previous works in the phytopathology laboratory of the Universidad Militar Nueva Granada. These miRNAs were validated, and their post-transcriptional control profile was related to the response mechanism of both varieties. Both strains were found to respond through the same post-transcriptional control profile and have a similar response mechanism. However, 395 is more efficient in its post-transcriptional control effort, controlling the appropriate genes to respond effectively. While SH2 does not have the same efficiency, which does not allow it to be timely in response to Fod. A total of 84 putative miRNAs are proposed that are controlling the expression mechanisms of 26 of the 35 genes at the time of elicitation. Performing "Knock out" or "Knock down" experiments on these 84 miRNAs can show loss or gain of resistance to Fod, which would allow us to conclude that these miRNAs are key to the response mechanism of carnation resistant varieties to Fod.Pregradoapplicaction/pdfspahttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 InternationalAcceso abiertoReconocimiento de miRNAs y su relación con la expresión de genes de clavel (Dianthus caryophyllus) involucrados en la respuesta al patógeno Fusarium oxysporum f. sp. dianthiRecognition of miRNAs and their relationship with the expression of carnation (Dianthus caryophyllus) genes involved in the response to the pathogen Fusarium oxysporum f. sp. dianthiTesis/Trabajo de grado - Monografía - Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1fmiRNACarnationFusarium oxysporumDianthus caryophyllusFUSARIUM OXYSPORUMDIANTHUS CARYOPHYLLUSORGANISMOS PATOGENOSmiARNClavelFusarium oxysporumDianthus caryophyllusBiología AplicadaFacultad de Ciencias BásicasUniversidad Militar Nueva Granada1. 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Microrna, 7(1), 4-10.Campus UMNGLICENSElicense.txtlicense.txttext/plain; charset=utf-83420http://repository.unimilitar.edu.co/bitstream/10654/44529/2/license.txta609d7e369577f685ce98c66b903b91bMD52open accessORIGINALMoralesVillanuevaCarlosAlberto2022.pdfMoralesVillanuevaCarlosAlberto2022.pdfTrabajo de gradoapplication/pdf1808588http://repository.unimilitar.edu.co/bitstream/10654/44529/1/MoralesVillanuevaCarlosAlberto2022.pdf75179854a615c0434a5a81e321183854MD51open access10654/44529oai:repository.unimilitar.edu.co:10654/445292023-06-13 15:01:04.905open accessRepositorio Institucional 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