Cytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approaches
Cancer is a major cause of death and an impediment to increasing life expectancy worldwide. With the aim of finding new molecules for chemotherapeutic treatment of epidemiological relevance, ten alkaloid fractions from Amaryllidaceae species were tested against six cancer cell lines (AGS, BT-549, HE...
- Autores:
-
Trujillo, Lina
Bedoya, Janeth
Cortés, Natalie
Osorio, Edison H.
Gallego, Juan-Carlos
Leiva, Hawer
Castro, Dagoberto
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2023
- Institución:
- Universidad de Ibagué
- Repositorio:
- Repositorio Universidad de Ibagué
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unibague.edu.co:20.500.12313/3839
- Acceso en línea:
- https://hdl.handle.net/20.500.12313/3839
- Palabra clave:
- Amaryllidaceae alkaloids
Cancer
Cytotoxic activity
In silico assays
- Rights
- openAccess
- License
- http://purl.org/coar/access_right/c_abf2
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Cytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approaches |
| title |
Cytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approaches |
| spellingShingle |
Cytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approaches Amaryllidaceae alkaloids Cancer Cytotoxic activity In silico assays |
| title_short |
Cytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approaches |
| title_full |
Cytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approaches |
| title_fullStr |
Cytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approaches |
| title_full_unstemmed |
Cytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approaches |
| title_sort |
Cytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approaches |
| dc.creator.fl_str_mv |
Trujillo, Lina Bedoya, Janeth Cortés, Natalie Osorio, Edison H. Gallego, Juan-Carlos Leiva, Hawer Castro, Dagoberto |
| dc.contributor.author.none.fl_str_mv |
Trujillo, Lina Bedoya, Janeth Cortés, Natalie Osorio, Edison H. Gallego, Juan-Carlos Leiva, Hawer Castro, Dagoberto |
| dc.subject.proposal.eng.fl_str_mv |
Amaryllidaceae alkaloids Cancer Cytotoxic activity In silico assays |
| topic |
Amaryllidaceae alkaloids Cancer Cytotoxic activity In silico assays |
| description |
Cancer is a major cause of death and an impediment to increasing life expectancy worldwide. With the aim of finding new molecules for chemotherapeutic treatment of epidemiological relevance, ten alkaloid fractions from Amaryllidaceae species were tested against six cancer cell lines (AGS, BT-549, HEC-1B, MCF-7, MDA-MB 231, and PC3) with HaCat as a control cell line. Some species determined as critically endangered with minimal availability were propagated using in vitro plant tissue culture techniques. Molecular docking studies were carried out to illustrate binding orientations of the 30 Amaryllidaceae alkaloids identified in the active site of some molecular targets involved with anti-cancer activity for potential anti-cancer drugs. In gastric cancer cell line AGS, the best results (lower cell viability percentages) were obtained for Crinum jagus (48.06 ± 3.35%) and Eucharis bonplandii (45.79 ± 3.05%) at 30 µg/mL. The research focused on evaluating the identified alkaloids on the Bcl-2 protein family (Mcl-1 and Bcl-xL) and HK2, where the in vitro, in silico and statistical results suggest that powelline and buphanidrine alkaloids could present cytotoxic activity. Finally, combining experimental and theoretical assays allowed us to identify and characterize potentially useful alkaloids for cancer treatment |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-10-17T20:51:21Z |
| dc.date.available.none.fl_str_mv |
2023-10-17T20:51:21Z |
| dc.date.issued.none.fl_str_mv |
2023-03-13 |
| dc.type.none.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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Trujillo, L.; Bedoya, J.; Cortés, N.; Osorio, E.H.; Gallego, J.-C.; Leiva, H.; Castro, D.; Osorio, E. Cytotoxic Activity of Amaryllidaceae Plants against Cancer Cells: Biotechnological, In Vitro, and In Silico Approaches. Molecules 2023, 28, 2601. https://doi.org/10.3390/ molecules28062601 |
| dc.identifier.issn.none.fl_str_mv |
14203049 |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12313/3839 |
| identifier_str_mv |
Trujillo, L.; Bedoya, J.; Cortés, N.; Osorio, E.H.; Gallego, J.-C.; Leiva, H.; Castro, D.; Osorio, E. Cytotoxic Activity of Amaryllidaceae Plants against Cancer Cells: Biotechnological, In Vitro, and In Silico Approaches. Molecules 2023, 28, 2601. https://doi.org/10.3390/ molecules28062601 14203049 |
| url |
https://hdl.handle.net/20.500.12313/3839 |
| dc.language.iso.none.fl_str_mv |
eng |
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eng |
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18 |
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2601 |
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1 |
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28 |
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Molecules |
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Trujillo, Linadb35c764-d73e-4393-a97f-e865a3d81421-1Bedoya, Janeth82bfd55c-e000-4b86-b5e0-e6d35cc01c8a-1Cortés, Natalie16e73ae2-5c91-425c-9ce1-5026766280f8-1Osorio, Edison H.087e0c0b-d49f-4915-b7fa-272d785c30af-1Gallego, Juan-Carlos0683bcdc-78df-419a-ba22-20fdc9838eeb-1Leiva, Hawer5f850761-9909-4bb3-8838-980981259973-1Castro, Dagoberto58722899-215a-4344-a1ba-8b28813c3ec1-12023-10-17T20:51:21Z2023-10-17T20:51:21Z2023-03-13Cancer is a major cause of death and an impediment to increasing life expectancy worldwide. With the aim of finding new molecules for chemotherapeutic treatment of epidemiological relevance, ten alkaloid fractions from Amaryllidaceae species were tested against six cancer cell lines (AGS, BT-549, HEC-1B, MCF-7, MDA-MB 231, and PC3) with HaCat as a control cell line. Some species determined as critically endangered with minimal availability were propagated using in vitro plant tissue culture techniques. Molecular docking studies were carried out to illustrate binding orientations of the 30 Amaryllidaceae alkaloids identified in the active site of some molecular targets involved with anti-cancer activity for potential anti-cancer drugs. In gastric cancer cell line AGS, the best results (lower cell viability percentages) were obtained for Crinum jagus (48.06 ± 3.35%) and Eucharis bonplandii (45.79 ± 3.05%) at 30 µg/mL. The research focused on evaluating the identified alkaloids on the Bcl-2 protein family (Mcl-1 and Bcl-xL) and HK2, where the in vitro, in silico and statistical results suggest that powelline and buphanidrine alkaloids could present cytotoxic activity. Finally, combining experimental and theoretical assays allowed us to identify and characterize potentially useful alkaloids for cancer treatmentapplication/pdfTrujillo, L.; Bedoya, J.; Cortés, N.; Osorio, E.H.; Gallego, J.-C.; Leiva, H.; Castro, D.; Osorio, E. 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Chem. 1998, 19, 1639–1662This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacional (CC BY 4.0)https://creativecommons.org/licenses/by-nc-nd/4.0/https://www.mdpi.com/1420-3049/28/6/2601Amaryllidaceae alkaloidsCancerCytotoxic activityIn silico assaysCytotoxic activity of Amaryllidaceae plants against cancer cells : biotechnological, in vitro, and in silico approachesArtículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Textinfo:eu-repo/semantics/articlehttp://purl.org/redcol/resource_type/ARTinfo:eu-repo/semantics/publishedVersionPublicationTEXTCytotoxic Activity of Amaryllidaceae Plants against Cancer Cells Biotechnological, In Vitro, and In Silico Approaches - molecules-28-02601.pdf.txtCytotoxic Activity of Amaryllidaceae Plants against Cancer Cells Biotechnological, In Vitro, and In Silico Approaches - molecules-28-02601.pdf.txtExtracted texttext/plain4540https://repositorio.unibague.edu.co/bitstreams/160a92db-b76a-4464-bd13-e2b135b48f08/download0ca4ac20a2361b3f2961c3e5ba016eeeMD53THUMBNAILCytotoxic Activity of Amaryllidaceae Plants against Cancer Cells Biotechnological, In Vitro, and In Silico Approaches - molecules-28-02601.pdf.jpgCytotoxic Activity of Amaryllidaceae Plants against Cancer Cells Biotechnological, In Vitro, and In Silico Approaches - molecules-28-02601.pdf.jpgGenerated Thumbnailimage/jpeg12280https://repositorio.unibague.edu.co/bitstreams/2ff33373-a50d-4635-8c82-dd5f7372d51a/download8eb025e584b2738ead2f7efff1f37852MD54ORIGINALCytotoxic Activity of Amaryllidaceae Plants against Cancer Cells Biotechnological, In Vitro, and In Silico Approaches - molecules-28-02601.pdfCytotoxic Activity of Amaryllidaceae Plants against Cancer Cells Biotechnological, In Vitro, and In Silico Approaches - molecules-28-02601.pdfapplication/pdf86599https://repositorio.unibague.edu.co/bitstreams/88b430cb-defe-42bf-8c45-c46cdb7a745f/downloadd3f123285b9b2ba52633cf6aec2439edMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-8134https://repositorio.unibague.edu.co/bitstreams/9562c557-dc6f-4638-9b1e-0aa8ae9d5bb1/download2fa3e590786b9c0f3ceba1b9656b7ac3MD5220.500.12313/3839oai:repositorio.unibague.edu.co:20.500.12313/38392023-10-18 03:00:46.96https://creativecommons.org/licenses/by-nc-nd/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).https://repositorio.unibague.edu.coRepositorio Institucional Universidad de Ibaguébdigital@metabiblioteca.comQ3JlYXRpdmUgQ29tbW9ucyBBdHRyaWJ1dGlvbi1Ob25Db21tZXJjaWFsLU5vRGVyaXZhdGl2ZXMgNC4wIEludGVybmF0aW9uYWwgTGljZW5zZQ0KaHR0cHM6Ly9jcmVhdGl2ZWNvbW1vbnMub3JnL2xpY2Vuc2VzL2J5LW5jLW5kLzQuMC8= |